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Actinomycetales (14):
Recent article
Actinomycetales
Journal of Antibiotics (63)12 2010
Abstract
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Journal of Antibiotics (63)11 2010
Abstract
We screened actinomycetes capable of converting AS1387392 to AS1429716 and identified those strains capable of hydroxylation. Amycolatopsis azurea JCM 3275 was found to be a particularly efficient strain, capable of converting AS1387392 to AS1429716, with a yield of 44% after 9 h. This strain can...
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PMID: 20924384
PDF is available here.
PDF is available here.
Journal of Antibiotics (63)11 2010
Abstract
We cloned an entire novel P450 gene (1209 bp) and named it P450Um-1. Its deduced amino acid sequence was homologous with that of the CYP105 subfamily. Further cloning of the upstream region, which may contain the native promoter site, was followed by insertion of the open reading frame with the up...
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PMID: 20924385
PDF is available here.
PDF is available here.
Abstract
I (ID03-0561(T)) showed low relatedness to the other strains studied. The three strains in group II (ID03-0784(T), ID03-0808 and ID03-0809) formed a group with high relatedness (98-100 %) and showed low relatedness to the other strains studied. The two strains in group III (ID03-0810(T) and ID03-0...
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PMID: 19933586
PDF is available here.
PDF is available here.
Abstract
A polyphasic taxonomic study of a halotolerant bacterium, isolated from sandy rhizospheric soil in Sarbandar, Persian Gulf, Iran, revealed that strain HM6(T) represents a novel species within the genus Nocardiopsis. Results of the 16S rRNA gene sequence comparison revealed that strain HM6(T) cluster...
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PMID: 19933587
PDF is available here.
PDF is available here.
Abstract
These results suggested that strain VN05A0561(T) represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia babensis sp. nov. is proposed. The type strain is VN05A0561(T) (=VTCC-A-1757(T)=NBRC 105793(T))....
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PMID: 19933589
PDF is available here.
PDF is available here.
Abstract
A Gram-stain-positive, aerobic bacterium, designated strain BZ41(T), was isolated from hydrocarbon-contaminated soil. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ41(T) was related to members of the genus Agromyces and showed highest similarity with the type strain of...
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PMID: 19933590
PDF is available here.
PDF is available here.
Abstract
A short coccoid- to rod-shaped, motile, mesophilic actinobacterium, strain MSL-22(T), was isolated from soil on Bigeum Island, Korea. A polyphasic study was undertaken to establish the taxonomic position of this strain. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MSL-...
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PMID: 19915109
PDF is available here.
PDF is available here.
Abstract
An actinobacterial strain was isolated from subarctic forest soil, and subjected to polyphasic taxonomic characterization. The cell-wall peptidoglycan comprised meso-diaminopimelic acid, alanine and glutamic acid. MK-9(H₄) was the predominant isoprenoid quinone, and iso-C₁₇:₀, iso-C₁₅:...
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PMID: 19946054
PDF is available here.
PDF is available here.
Abstract
Estrogen receptor ERalpha and ERbeta heterodimerization has been implicated in cancer chemoprevention. The discovery, structural elucidation, and total synthesis of a new natural product, actinopolymorphol A (1), from Actinopolymorpha rutilus (YIM45725) that preferentially induces ERalpha/beta heter...
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PMID: 20593804
PDF is available here.
PDF is available here.
Antonie Van Leeuwenhoek (98)2 2010
Abstract
We reconstruct the phylogenetic relationships within the bacterial genus Pseudonocardia to evaluate two models explaining how and why Pseudonocardia bacteria colonize the microbial communities on the integument of fungus-gardening ant species (Attini, Formicidae). The traditional Coevolution-Codiver...
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PMID: 20333466
PDF is available here.
PDF is available here.
Abstract
We assessed the effect of novel immunotherapeutic heat-killed bacterial (Actinomycetales) preparations on the development of myointimal hyperplasia (MIH) in a rat carotid balloon trauma model and the effect on the immune response by measuring the expression of interferon gamma (IFN-gamma; (Th1) and...
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PMID: 20498142
PDF is available here.
PDF is available here.
Abstract
The aerobic metabolism of monofluorophenols (mono-FPs) by the actinomycete, Pseudonocardia benzenivorans, was studied. This strain was able to grow on 4-fluorophenol (4-FP) and readily transform 2- and 3-fluorophenol to the corresponding metabolites. The detailed mechanism of mono-FPs degradation by...
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PMID: 20473664
PDF is available here.
PDF is available here.
Journal of Antibiotics (63)7 2010
Abstract
Abstract
Three new antibiotics were isolated from the fermentation broth of Saccharothrix algeriensis NRRL B-24137 and characterized as the dithiolopyrrolone derivatives valerylpyrrothine (1), isovalerylpyrrothine (2), and formylpyrrothine (3) as well as the known antibiotic aureothricin. The production of t...
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PMID: 20507156
PDF is available here.
PDF is available here.
Antonie Van Leeuwenhoek (98)1 2010
Abstract
A Gram-positive, moderately halophilic, facultatively alkaliphilic, catalase- and oxidase-positive, obligately aerobic, filamentous actinomycete strain, designated YIM 90022(T), was isolated from saline soil collected from the Qaidam Basin, north-west China. Phylogenetic analysis based on 16S rRNA g...
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PMID: 20232249
PDF is available here.
PDF is available here.
Antonie Van Leeuwenhoek (98)1 2010
Abstract
During the course of our research on new actinobacterial sources, a novel actinomycete strain YIM 67013(T) was isolated from rhizosphere soil of Tripterygium wilfordii Hook. f. collected from Yunnan province, south-west China. The strain formed well-differentiated aerial and substrate mycelia, grew...
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PMID: 20354787
PDF is available here.
PDF is available here.
Journal of Antibiotics (63)6 2010
Abstract
The time-kill studies using pargamicin A against Staphylococcus aureus and Enterococcus faecalis were performed. The effects of the incorporation of radioactive precursors into macromolecules, membrane potential and function using fluorescent dyes were also examined. These studies revealed that rapi...
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PMID: 20339400
PDF is available here.
PDF is available here.
Abstract
Rhodococcus equi and Dietzia spp. are closely related actinomycetes that show similar phenotypic properties. In humans, R. equi is an opportunistic pathogen associated with severe immunodeficiency. Dietzia spp. are environmental bacteria that have been isolated recently from clinical material and ar...
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PMID: 20220156
PDF is available here.
PDF is available here.
Proteomics (10)7 2010
Abstract
A differential proteomic analysis, based on 2-DE and MS procedures, was performed on Amycolatopsis balhimycina DSM5908, the actinomycete producing the vancomycin-like antibiotic balhimycin. A comparison of proteomic profiles before and during balhimycin production characterized diffe...
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PMID: 20049855
PDF is available here.
PDF is available here.
Biotechnology Letters (32)4 2010
Abstract
A single-compartmented microbial fuel cell composed of a graphite felt anode modified with Neutral Red (NR-anode) and a porous Fe(II)-carbon cathode (FeC-cathode) were compared for electricity generation from Microbacterium sp. and Pseudomonas sp. under identical conditions. Pseudomonas sp. was more...
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PMID: 20013300
PDF is available here.
PDF is available here.
Abstract
Frequent "contaminants" detected during mycobacterial culture of decontaminated samples are bacteria of the order Actinomycetales. These are usually bacteria classified as the family Corynebacterineae, genera Corynebacterium, Dietzia, Gordonia, Nocardia, Rhodococcus and Tsukamurella. These bacteria...
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PMID: 20503156
PDF is available here.
PDF is available here.
Abstract
A novel Gram-stain-positive bacterium, designated R19-04(T), was isolated from a soil sample from Rishiri Island, Japan, and its taxonomic position was investigated by a polyphasic approach. Cells of strain R19-04( T) exhibited a rod-coccus cycle. The peptidoglycan type of the isolat...
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PMID: 19656927
PDF is available here.
PDF is available here.
Abstract
Tsukamurella exhibits laboratory similarities to mycobacteria and should be considered in the differential of atypical infection of the ocular surface....
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PMID: 20098312
PDF is available here.
PDF is available here.
Abstract
Abstract
The arsenic resistance gene cluster of Microbacterium sp. A33 contains a novel pair of genes (arsTX) encoding a thioredoxin system that are cotranscribed with an unusual arsRC2 fusion gene, ACR3, and arsC1 in an operon divergent from arsC3. The whole ars gene cluster is required to complement an Esc...
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PMID: 19966021
PDF is available here.
PDF is available here.
Abstract
Dermatophilosis caused by Dermatophilus congolensis causes exudative dermatitis in a variety of species. The infection is generally limited to the cutaneous tissues, where infection is acquired from the environment and bacteria penetrate into keratinized epithelium through epithelial disruption. A 3...
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PMID: 20093705
PDF is available here.
PDF is available here.
Abstract
The effects of different structural features on the thermostability of Thermopolyspora flexuosa xylanase XYN10A were investigated. A C-terminal carbohydrate binding module had only a slight effect, whereas a polyhistidine tag increased the thermostability of XYN10A xylanase. In contrast, glycosylati...
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PMID: 19854928
PDF is available here.
PDF is available here.
Abstract
6-Beta-halogenopenicillanates are powerful, irreversible inhibitors of various beta-lactamases and penicillin-binding proteins. Upon acylation of these enzymes, the inhibitors are thought to undergo a structural rearrangement associated with the departure of the iodide and formation of a dihydrothia...
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PMID: 19919161
PDF is available here.
PDF is available here.
Abstract
We report aromatic boronic acid inhibitors of the D,D-carboxypeptidase R39 from Actinomadura sp. strain. Analogues of an initially identified inhibitor [3-(dihydroxyboryl)benzoic acid 1, IC(50) 400 microM] were prepared via routes involving pinacol boronate esters, which were deprotected via a two-s...
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PMID: 19731939
PDF is available here.
PDF is available here.
Journal of Antibiotics (62)10 2009
Abstract
A novel isoquinoline alkaloid of molecular formula C10H9NO4, labeled JS-1, was isolated from the culture broth of Streptomyces sp. 8812. It was purified by acetone protein precipitation from the culture supernatant, followed by anion exchange and C18 RP HPLC columns. JS-1 is an inhibitor of exocellu...
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PMID: 19713994
PDF is available here.
PDF is available here.
Abstract
Strain RuGl7(T) was isolated from a soil sample collected at the periphery of the glacial Lake Roopkund in the Himalayan mountain range, India. Cells of RuGl7(T) were Gram-positive, aerobic, rod-shaped, motile and grew optimally between 15 and 18 degrees C. Cells of RuGl7(T) containe...
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PMID: 19661491
PDF is available here.
PDF is available here.
Abstract
Three Gram-positive, rod-shaped bacteria (strains CC-SBCK-209( T), CC-12309(T) and CC-5209(T)) were isolated from the stalk of the edible mushroom Agaricus blazei grown in the laboratory. 16S rRNA gene sequence analysis indicated that all three isolates clearly belonged to the genus...
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PMID: 19661494
PDF is available here.
PDF is available here.
Abstract
A Gram-positive-staining, non-motile, rod- or coccoid-shaped bacterium, strain CLG(T), was isolated from beach sand from Lianyungang, Jiangsu Province, PR China. The organism grew optimally at 30 degrees C and pH 7.0. Strain CLG(T) contained L-Lys-D-Asp as the peptidoglycan type and...
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PMID: 19661504
PDF is available here.
PDF is available here.
Abstract
A novel actinomycete strain, designated MS498(T), was isolated from an ocean sediment sample collected from the South China Sea. It was subjected to a polyphasic analysis to determine its taxonomic position. The phylogenetic tree based on 16S rRNA gene sequences showed that strain MS...
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PMID: 19666793
PDF is available here.
PDF is available here.
Abstract
We describe the first report of construction and characterization of a transposon (Tn) mutant library of C. michiganensis subsp. sepedonicus sp. strain R10. Since virulence of R10 in potato has been shown previously to be associated with elicitation of a nonhost hypersensitive response (HR), the mut...
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PMID: 19522563
PDF is available here.
PDF is available here.
Abstract
A new isofuranonaphthoquinone, 7,8-dihydroxy-1-methylnaphtho[2,3-c]furan-4,9-dione, was isolated from cultures of an Actinoplanes isolate obtained using an in situ diffusion technology that facilitates the isolation of soil microorganisms. This compound was demonstrated to have the ability to comple...
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PMID: 19449881
PDF is available here.
PDF is available here.
Abstract
We found the best isolation approach involved Dispersion and Differential Centrifugation (DDC) pretreatment and GTV medium supplemented with cycloheximide, nalidixic acid nystatin (each at 50 mg/L). 369 isolates were obtained, and were assigned to 10 color groups. 6.6% of the isolates were strictly...
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PMID: 19673405
PDF is available here.
PDF is available here.
Abstract
Thiazolyl peptides are a class of highly rigid trimacrocyclic compounds consisting of varying but large numbers of thiazole rings. The need for new antibacterial agents to treat infections caused by resistant bacteria prompted a reinvestigation of this class, leading to the previous isolation of thi...
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PMID: 19334707
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (293)1 2009
Abstract
An actinomycete strain, Amycolatopsis sp. CsO-2, produces a 27-kDa chitosanase. To reveal the molecular characteristics of the enzyme, its corresponding gene ctoA was cloned by a reverse genetic technique, based on the N-terminal amino acid sequence of the protein. The encoded CtoA protein was deduc...
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PMID: 19236484
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)12 2009
Abstract
XPB, the largest subunit of the eukaryotic transcription factor TFIIH, is essential for both initiation of transcription by RNA polymerase II and nucleotide excision repair (NER). XPB belongs to the SF2 superfamily of monomeric helicases. XPB helicase is thought to have evolved in eukaryotes; howeve...
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PMID: 19199647
PDF is available here.
PDF is available here.
Journal of Antibiotics (62)4 2009
Abstract
Journal of Antibiotics (62)3 2009
Abstract
Ammocidins B, C and D were isolated from the culture broth of Saccharothrix sp. AJ9571, an ammocidin A-producing strain. Their structures were determined by a detailed spectroscopic analysis and by a comparison of their NMR data with those of ammocidin A. Ammocidins A and B showed potent anti-prolif...
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PMID: 19198629
PDF is available here.
PDF is available here.
Journal of Antibiotics (62)2 2009
Abstract
We applied a DNA hybridization assay (dot blot method) for screening. Using this method, we screened microbial products and found the polyether compound CP-44161 from the culture broth of an actinomycete strain. CP-44161 was previously reported as an anticoccidal agent, but there has been no claim o...
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PMID: 19198636
PDF is available here.
PDF is available here.
Abstract
The genus Dietzia has only been established fairly recently. The Gram morphology and colony appearance of the species of this genus is remarkably similar to Rhodococcus equi. In the absence of simple, accurate methods for their identification, Dietzia spp. might have been misidentified as a Rhodococc...
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PMID: 19159434
PDF is available here.
PDF is available here.
Annals of Internal Medicine (150)2 2009
Abstract
Abstract
A 45-year-old male presented with history of multiple swellings over the foot with sinuses discharging seropurulent pus. Actinomadura madurae was demonstrated and identified by microbiological culture from the pus obtained directly of the lesion. This case is reported to emphasize th...
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PMID: 19749452
PDF is available here.
PDF is available here.
Abstract
It was established, that the main cause of bacterial tomato wilting in the fields and greenhouses is Clavibacter michiganensis subsp. michiganensis, the agent of tomato bacterial canker. It is especially dangerous in the greenhouse soil, where it causes the mass plant diseases in the fruiting period...
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PMID: 20458935
PDF is available here.
PDF is available here.
Abstract
Thirteen poly (L-lactide)-degrading microorganisms were isolated and selected based on their ability of clear zone formation on an emulsified PLA agar plate and the enzyme activity in culture broth. According to phenotypic properties and 16S rRNA gene sequence, these strains were classified to vario...
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PMID: 20118610
PDF is available here.
PDF is available here.
Abstract
An actinobacterial strain, 2SbT, isolated from lichen was characterized taxonomically using a polyphasic approach. Strain 2SbT was Gram-positive, strictly aerobic, rod-shaped and non-motile. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain 2SbT was located in the genus Leif...
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PMID: 19940379
PDF is available here.
PDF is available here.
Abstract
Molecular docking and molecular dynamics (MD) simulations were used to investigate the binding of a cellodextrin chain in a crystal-like conformation to the carbohydrate-binding module (CBM) of Cel9A from Thermobifida fusca. The fiber was found to bind to the CBM in a single and well-defined configu...
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PMID: 18853469
PDF is available here.
PDF is available here.
Abstract
We constructed a heterologous expression system for AhDOs in Rhodococcus species using a constitutive expression promoter, P(dfdB), and a shuttle vector, pRK401, and analyzed the ability of these enzymes to degrade dibenzofuran and deplete several chlorinated dioxins. Three active AhDOs expressed in...
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PMID: 19352040
PDF is available here.
PDF is available here.
Mikrobiologiia (78)6 2009
Abstract
Phosphobacteria are able to enhance phosphorus availability in soil and improve crop yields. To develop such biofertilizers, 14 predominant phosphobacteria were isolated from eutrophic aquatic ecosystems. Molecular identification and phylogenetic analysis revealed three groups among the nine isolate...
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PMID: 20170019
PDF is available here.
PDF is available here.
Abstract
A new biologically active component, antibiotic eremomycin B, was isolated from the culture liquid of Amycolatopsis orientalis subsp. eremomycini, the producing strain for antibiotic eremomycin. Its structure was established by NMR spectroscopy and mass spectrometry. Eremomycin B was shown to differ...
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PMID: 19928058
PDF is available here.
PDF is available here.
Abstract
A novel Gram-positive, aerobic, short-rod-shaped bacterium, designated strain J112T, was isolated from black sand collected from Soesoggak, Jeju Island, Korea. The strain was found to be oxidase-negative and catalase-positive. Cells grew at 10-37 degrees C, at pH 5.5-8.0 and with 1-10 % NaCl. Growth...
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PMID: 19126721
PDF is available here.
PDF is available here.
Abstract
A yellow-pigmented actinobacterium, designated strain MSL-13T, was isolated from a soil sample collected from Bigeum Island, Republic of Korea, and its taxonomic position was determined by using a polyphasic approach. Strain MSL-13T showed phenotypic and chemotaxonomic properties consistent with its...
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PMID: 19126716
PDF is available here.
PDF is available here.
BMC Microbiology (9)1 2009
Abstract
A total of 58 arsenite-resistant bacteria were identified from soils with three different arsenic-contaminated levels. Highly arsenite-resistant bacteria (MIC > 20 mM) were only isolated from the highly arsenic-contaminated site and belonged to Acinetobacter, Agrobacterium, Arthrobacter, Comamonas,...
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PMID: 19128515
PDF is available here.
PDF is available here.
Journal of Bacteriology (191)1 2009
Abstract
Dibenzofuran (DF) is one of the dioxin carbon skeletal compounds used as a model to study the microbial degradation of dioxins. This study analyzed the transcriptional regulation of the DF dioxygenase genes dfdA1 to dfdA4 in the DF-utilizing actinomycetes Rhodococcus sp. strain YK2 and Terrabacter s...
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PMID: 18952799
PDF is available here.
PDF is available here.
Developmental Dynamics (237)12 2008
Abstract
We show here that bus-1 encodes a predicted acyltransferase expressed in rectal epithelial cells (K, F, and U), suggesting its involvement in regional surface modification. bus-1 reporter genes were used to show spatial regulation by hindgut developmental control genes: egl-38, mab-9, and mab-23. A...
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PMID: 19035336
PDF is available here.
PDF is available here.
Abstract
The taxonomic position of one soil isolate 13-12(50)(T) was clarified by a polyphasic study. The organism showed a combination of chemotaxonomic and morphological properties typical of the genus Actinomadura. It formed a distinct phyletic line in the Actinomadura 16S rRNA gene tree and was closely a...
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PMID: 19164876
PDF is available here.
PDF is available here.