Abstract
We identified 12 strains capable of efficient hydroxylation. Results of phylogenetic analysis of 16S rDNA sequences suggested that these strains belonged to the genera Lentzea, Saccharopolyspora, Sphaerisporangium and Amycolatopsis. Morphological and chemical characteristics as well as results of ph...
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PMID: 20664604
PDF is available here.
Abstract
Staphylococcus aureus is now amongst the most important pathogenic bacteria responsible for bloodstream nosocomial infections and for biofilm formation on indwelling medical devices. Its increasing resistance to common antibiotics, partly attributed to its ability to form biofilms, is a challenge fo...
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PMID: 20706890
PDF is available here.
Abstract
In this report, the diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve collected from a remote island of the South China Sea was investigated employing classical cultivation and characterization, 16S rDNA library construction, 16S rDNA-restriction fragment length poly...
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PMID: 20383659
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Abstract
Microbial communities in sewage sludge and green waste co-composting were investigated using culture-dependent methods and community level physiological profiles (CLPP) with Biolog Microplate. Different microbial groups characterized each stage of composting. Bacterial densities were high from begin...
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PMID: 20060702
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Abstract
We investigated the effects of two bulking materials, Sphagnum peat and pine wood chips, on the early stages of biowaste composting in two pilot-scale processes. Emphasis was placed on studying the formation conditions of malodorous compost gases in the initial phases of the processes. The results s...
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PMID: 20061130
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Abstract
A biological comparison based on differences in the starting material for composting processes was made. Mesophilic aerobic microbiota, fungi, actinomycetes and hemicellulolytic microorganisms reached significantly higher levels in the MSW final product. The population of cellulolytic microorganisms...
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PMID: 20096556
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Abstract
New terpenoids named gifhornenolones A (1) and B (2) were isolated from the culture broth of Verrucosispora gifhornensis YM28-088, and their structures were established as hydroxylated isopimaradiene derivatives on the basis of extensive NMR and MS spectral analyses. In addition, a known sesquiterpe...
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PMID: 20379218
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Abstract
We discovered the streptogramin etamycin produced by an actinomycete species isolated from the coast of Fiji, the first time this antibiotic has been identified from a marine microbe. Etamycin was extracted and purified from this strain (CNS-575) and identified as a three-rotamer species by 2D NMR s...
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PMID: 20339399
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Abstract
About 64 total actinomycetes were isolated from various coastal soils. Sixteen actinomycete isolates were screened for herbicidal principles. Out of these, five potent isolates were selected for characterisation and identification. Based on their morphological, biochemical and physiological characte...
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PMID: 20182949
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Abstract
The growing drug resistance of Plasmodia spp. to current antimalarial agents in the quinine and artemisinin families further asserts the need for novel drug classes to combat malaria infection. One approach to the discovery of new antimalarials is the screening of natural product extracts for activi...
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PMID: 20230372
PDF is available here.
Maria Schubert,
Anna N Melnikova,
Nikola Mesecke,
Elena K Zubkova,
Rocco Fortte,
Denis R Batashev,
Inga Barth,
Norbert Sauer,
Yuri V Gamalei,
Natalia S Mamushina,
Lutz F Tietze,
Olga V Voitsekhovskaja and
Katharina Pawlowski
Abstract
Datisca glomerata forms nitrogen-fixing root nodules in symbiosis with soil actinomycetes from the genus Frankia. Analysis of sugars in roots, nodules and leaves of D. glomerata revealed the presence of two novel compounds that were identified as alpha-L-rhamnopyranoside-(1 --> 6)-D-glucose (rutinos...
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PMID: 19915863
PDF is available here.
Abstract
The ability of the actinomycetes and coryneform bacteria isolated from the root tissues of winter rye to produce auxin in a liquid culture was studied. The isolates of coryneform bacteria produced indolyl-3-acetic acid (IAA) into the medium in the amount of 9.0-95.0 microg/ml and the isolates of act...
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PMID: 20198917
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Abstract
Concurrent samples were collected with Andersen and IOM foam samplers to determine whether if the IOM foam sampler can be applied to collect culturable microorganisms. Two different kinds of aerosols were studied: peat dust in a power plant and mist from coolant fluid aerosolized during grinding of...
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PMID: 19904652
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Abstract
The newly isolated strain E1, identified as a Dietzia sp., proved to have an excellent ability to degrade n-C12 to n-C38 alkane components of crude oil. The preferred substrate was the very long-chain alkane n-eicosane at an optimal temperature of 37 degrees C and an optimal pH of 8...
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PMID: 21319712
PDF is available here.
Abstract
Bacterial abundance and diversity in snow of East Rongbuk, Laohugou and Hailuogou glaciers on the Tibetan Plateau were investigated through epifluorescence microscope and denaturing gradient gel electrophoresis. Cell abundance ranged from 4.0 x 10(3) to 290.2 x 10(3) cells/mL. The phylogenetic trees...
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PMID: 21174974
PDF is available here.
Abstract
Foaming incidents in activated sludge treatment plants are a worldwide problem and occur on a regular basis in both municipal and industrial activated sludge treatment plants. Foaming is most often caused by excessive growth of filamentous bacteria, especially the gram-positive ones affiliated withi...
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PMID: 20453316
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Abstract
We report the first case of Gordonibacter pamelaeae bacteremia, identified by phenotypic tests and 16S rRNA sequencing in a patient with disseminated rectosigmoid carcinoma and responsive to amoxicillin-clavulanate. The bacterium was a nonsporulating, anaerobic, gram-positive, nonmotile, coccobacill...
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PMID: 19923481
PDF is available here.
Abstract
We present an initial survey of the V. destructor genome carried out to advance our understanding of Varroa biology and to identify new avenues for mite control. This sequence survey provides immediate resources for molecular and population-genetic analyses of Varroa-Apis interactions and defines th...
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PMID: 20973996
PDF is available here.
Abstract
We describe a case of spondylodiscitis due to Eggerthella lenta in an 82-year-old Chinese woman presenting with back pain. The organism was isolated from tissue cultures obtained via radiology-guided biopsy....
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PMID: 20087537
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Abstract
OBJECTIVE: To study the characteristics of soil microbial variation during Salvia miltiorrhiza crop rotation. METHOD: the conventional cultivating microbial method was used to study the microbial number and communities structure and soil microbial biomass phosphorus (SMBP) was determined by chlorofo...
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PMID: 20352995
PDF is available here.
Abstract
We generated a series of salinosporamide analogues with altered proteasome binding affinity. One of the engineered compounds is equipotent to salinosporamide A in inhibition of the chymotrypsin-like activity of the proteasome yet exhibits superior activity in the cell-based HCT-116 assay....
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PMID: 19746976
PDF is available here.
Abstract
Actinomycetes (121) from the samples were isolated on four media. Among them 47 strains belonged to 9 actinomycetes genera. Six known rare genera and one possible new genus of actinomyctes from Rhizomys sinensis were identified. Three possible new species from other three samples were identified. So...
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PMID: 20030051
PDF is available here.
Abstract
Marine ecosystems are a source of biologically active compounds, many of which are currently in clinical use. With the goal of increasing the availability and the chemical diversity of these important compounds, more researchers are applying the tools of biotechnology to the discovery and production...
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PMID: 19715117
PDF is available here.
Abstract
One strain F05 which had better antagonism for American ginseng rust rot was obtained from continuous cropping ground, and its fermentation had been preliminarily studied, more over the research can further determine the optimum composition. The single factor and uniform design were used to optimize...
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PMID: 20030072
PDF is available here.
Abstract
We examined the ability of different freshwater bacterial groups to take up leucine and thymidine in two lakes. Utilization of both substrates by freshwater bacteria was examined at the community level by looking at bulk incorporation rates and at the single-cell level by combining fluorescent in si...
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PMID: 19725866
PDF is available here.
Abstract
We cocultivated the important model fungus Aspergillus nidulans with a collection of 58 soil-dwelling actinomycetes. By microarray analyses of both Aspergillus secondary metabolism and full-genome arrays and Northern blot and quantitative RT-PCR analyses, we demonstrate at the molecular level that a...
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PMID: 19666480
PDF is available here.
Abstract
Six antitumor antibiotics of a new structure class, indoxamycins A-F (1-6), were isolated from a saline culture group of marine-derived actinomyces whose strains showed approximately 96% sequence homology of 16S rDNA with the family streptomycetaceae. The structures of these indoxamycins, which are...
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PMID: 19572603
PDF is available here.
Abstract
We disclose that there are more actinobacteria PHA producers in addition to the genera cited. Some unusual genera, such as Streptoalloteichus, and some genera frequently present in soil, such as Streptacidiphilus, have been found. Thirty-four isolates were able to accumulate poly(3-hydroxybutyrate)...
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PMID: 19767851
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Abstract
A total of 59 actinomycete strains were isolated from the marine sponge H. perlevis. A total of 27 representative actinomycetes were selected according to their morphological feature, color and pigments. They gave 15 different RFLP patterns after digesting their PCR products of 16s rDNA with Hha I....
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PMID: 19873748
PDF is available here.
Abstract
In this study, 137 actinomycetes were isolated from subtidal marine sediments in the North Pacific and Caribbean coasts of Costa Rica. Bioinformatics analysis of the 16S rRNA gene sequences assigned the isolates to 15 families and 21 genera. Streptomyces was the dominant genus while the remaining 20...
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PMID: 19365710
PDF is available here.
Abstract
We found the best isolation approach involved Dispersion and Differential Centrifugation (DDC) pretreatment and GTV medium supplemented with cycloheximide, nalidixic acid nystatin (each at 50 mg/L). 369 isolates were obtained, and were assigned to 10 color groups. 6.6% of the isolates were strictly...
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PMID: 19673405
PDF is available here.
Abstract
Brartemicin (1), a new trehalose-derived metabolite, was isolated from the culture broth of the actinomycete of the genus Nonomuraea. Its structure and absolute configuration were determined by spectroscopic analyses. The new compound inhibited the invasion of murine colon carcinoma 26-L5 cells with...
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PMID: 19358565
PDF is available here.
Abstract
A total of 63 actinomycetes were isolated from salt lake in Hami, of which 47 strains were halophilic actinomycetes. The antibacterial activity results showed that 23 strains had antibacterial activity toward Bacillus subtilis and other pathogens. Three strains produced proteinase, 46 strains produc...
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PMID: 19623950
PDF is available here.
Abstract
The microbial communities of freshwater hot spring mats from Boekleung (Western Thailand) were studied. Temperatures ranged from over 50 up to 57 degrees C. Green-, red-, and yellow colored mat layers were analyzed. In order to detect the major components of the microbial communities constituting th...
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PMID: 19109691
PDF is available here.
Abstract
Selective enrichments enabled the recovery of moderately thermophilic isolates with copper bioleaching ability from a spent copper sulfide heap. Phylogenetic and physiological characterization revealed that the isolates were closely related to Sulfobacillus thermosulfidooxidans, Acidithiobacillus ca...
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PMID: 19104861
PDF is available here.
Abstract
New molecular techniques have increased our understanding of the numerous bacteria associated with bacterial vaginosis, and a biofilm containing mostly Gardnerella and Atopobium vaginae, which can persist after treatment has been described. Suppressive treatment with metronidazole gel can suppress r...
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PMID: 19532085
PDF is available here.
Abstract
We describe the structure of a family 2 glycoside hydrolase, CsxA, from Amycolatopsis orientalis that has exo-beta-D-glucosaminidase (exo-chitosanase) activity. Analysis of a product complex (1.85 A resolution) reveals a unique negatively charged pocket that specifically accommodates the nitrogen of...
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PMID: 18976664
PDF is available here.
Abstract
Staurosporine was discovered at the Kitasato Institute in 1977 while screening for microbial alkaloids using chemical detection methods. It was during the same era that protein kinase C was discovered and oncogene v-src was shown to have protein kinase activity. Staurosporine was first isolated from...
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PMID: 19378767
PDF is available here.
Abstract
The objective of this study was to investigate the culturable psychrotolerant microbial community in groundwater from Seabee Hook, Antarctica. Shallow groundwater can be present in coastal regions at higher latitudes during the Antarctic summer. Perched groundwater atop ice-cemented permafrost occur...
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PMID: 19190697
PDF is available here.
Abstract
The 25 representative strains belonged to 6 suborders, 12 families and 15 genera of the order Actinomycetals. For NRPS and CYP genes screening, positive strains were 14 and 11, respectively. Among the 111 actinomycetes isolated under low-temperature conditions, 88% were psychrotroph strains, 12% wer...
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PMID: 19388272
PDF is available here.
Abstract
We set out to isolate, characterize and screen actinomycetes from soil and plant material collected from eight mangrove sites in China. Over 2,000 actinomycetes were isolated and of these approximately 20%, 5%, and 10% inhibited the growth of Human Colon Tumor 116 cells, Candida albicans and Staphyl...
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PMID: 19370169
PDF is available here.
Abstract
I or II inhibition, mitochondria permeabilization, inhibition of key enzymes involved in signal transduction like proteases, or cellular metabolism and in some cases by inhibiting tumor-induced angiogenesis. Marine organisms have attracted special attention in the last years for their ability to pro...
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PMID: 19597582
PDF is available here.
Abstract
The objective of this study was to examine the alkalithermophilic actinomycete communities in the subtropical environment of Jujuy, Argentina, characterized by sugarcane crops. Laceyella putida, Laceyella sacchari, Thermoactinomyces intermedius, Thermoactinomyces vulgaris and Thermoflavimicrobium di...
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PMID: 19623902
PDF is available here.
Abstract
Phosphobacteria are able to enhance phosphorus availability in soil and improve crop yields. To develop such biofertilizers, 14 predominant phosphobacteria were isolated from eutrophic aquatic ecosystems. Molecular identification and phylogenetic analysis revealed three groups among the nine isolate...
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PMID: 20170019
PDF is available here.
Abstract
The biomass of several groups of microbial communities occurring in three types of alluvial soils in the upper Selenga River estuary near the village of Murzino, Kabansk raion, Buryat Republic was studied. The general tendency in the dynamics of the biomass of the dominant groups of microorganisms i...
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PMID: 19239119
PDF is available here.
Abstract
Most Actinobacteria produce mycothiol as the major thiol. In addition to mycothiol Rhodococcus AD45 generates a substantial level of glutathione possibly using genes acquired in a lateral transfer. Instead of mycothiol, Rubrobacter radiotolerans and Rubrobacter xylanophilus produce glutathione, whos...
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PMID: 18719892
PDF is available here.
Abstract
Mycetomas are chronic, granulomatous, subcutaneous infections caused by either eumycetes fungi or actinomycetes bacteria, giving rise to eumycetomas and actinomycetomas, respectively. The disease is endemic in many tropical countries, and is characterized by slow progression with risks of bone and v...
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PMID: 19076788
PDF is available here.
Abstract
To achieve the effects of artificial wetland on the bacterial diversity, the culturable bacteria and total cell counts of three wetland cells, including sewage pond (SP), free surface wetland (SF) and subsurface flow wetland (SSF), were investigated using the traditional culture-dependent approach a...
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PMID: 19353894
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Abstract
Bacteria were identified from a large, seasonally flooded river (Paraná River, Brazil) and two floodplain habitats that were part of the same river system yet very different in nature: clearwater Garças Lagoon and the highly humic waters of Patos Lagoon. Bacterioplankton were collected during mid-...
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PMID: 18587611
PDF is available here.
Abstract
We have examined the nature of the macroamphiphiles present in the thermophilic actinomycetes Thermobifida fusca and Rubrobacter xylanophilus. Following hot-phenol-water extraction and purification by hydrophobic interaction chromatography, Western blotting with a monoclonal antibody against lipotei...
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PMID: 18931132
PDF is available here.
Abstract
Hundred and twenty microbial isolates could be isolated from different soil samples collected from different localities in Egypt. One of the actinomycete culture AZ-H-A5 from three cultures was found to produce a wide spectrum antimicrobial agent when cultivated on rice straw. The actinomycete AZ-H-...
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PMID: 20222575
PDF is available here.
Abstract
Two bacterial strains, CP153-3 and CP177-2T, were isolated from biological soil crusts on the Colorado Plateau, USA, and assigned to the genus Patulibacter, according to 16S rRNA gene sequence analysis and phenotypic characteristics. The strains were Gram-positive, aerobic and psychrotolerant and sh...
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PMID: 19126730
PDF is available here.
Abstract
I propose to establish the candidate species 'Candidatus Planktoluna difficilis', 'Candidatus Aquiluna rubra', 'Candidatus Flaviluna lacus', 'Candidatus Rhodoluna limnophila', 'Candidatus Rhodoluna planktonica', 'Candidatus Rhodoluna lacicola' and 'Candidatus Limnoluna rubra' for these taxa....
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PMID: 19126733
PDF is available here.
Abstract
In this report it is shown that the glnA1 and glnA2 encoded glutamine synthetase sequences were inherited from an Actinobacteria ancestor, while the glnA4 and glnA3 encoded GS sequences were sequentially acquired during Actinobacteria speciation. The glutamine synthetase sequences encoded by glnA4 a...
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PMID: 19245690
PDF is available here.
Abstract
Reconstruction of transcriptional regulatory networks of uncharacterized bacteria is a main challenge for the post-genomic era. Recent studies, including one in BMC Systems Biology, address this problem in the relatively underexplored actinobacteria clade, which includes major pathogenic and economi...
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PMID: 19435474
PDF is available here.
Abstract
Bacterial community structure and the effects of several environmental factors on the microbial community distribution were investigated in the sediment of the eutrophic Lake Xuanwu. Profiles of bacterial communities were generated using denaturing gradient gel electrophoresis (DGGE), and the result...
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PMID: 19096460
PDF is available here.