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Esculin (0):
Recent article
Esculin
Abstract
A Gram-stain-positive, neutrophilic, rod-shaped bacterium, strain A1g(T), was isolated from activated sludge of a bioreactor and was subjected to a polyphasic taxonomic characterization. The isolate grew in the presence of 0-17.0 % (w/v) NaCl and at pH 6.0-9.0; optimum growth was observed in the p...
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PMID: 19946056
PDF is available here.
PDF is available here.
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Abstract
The enzymatic esterification of esculin catalyzed by Candida antarctica lipase B (Novozym 435) was carried out in ionic liquid (IL)-organic solvent mixed systems in comparison with individual systems. The reaction behaviors in IL-organic solvents were systemically evaluated using acetone as a model...
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PMID: 19415928
PDF is available here.
PDF is available here.
Abstract
Human gut bacteria could completely convert aesculin into aesculetin in vitro. The biotransformation process occurred from 8 to 24 h post-incubation, with its highest activity was seen from 8 to 12 h. The in vitro process was much slower than the in vivo process. In contrast to the in vitro model, s...
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PMID: 19322928
PDF is available here.
PDF is available here.
Abstract
The aim of the research was to investigate the anti-inflammatory mechanism of Pulsatillae Decoction (PD), the levels of interleukin (IL)-6, IL-8, E-selectin, and thromboxane B(2) (TXB(2)) secreted by cultured rat intestinal microvascular endothelial cells (RIMECs) were determined after treatment wit...
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PMID: 19874221
PDF is available here.
PDF is available here.
Abstract
OBJECTIVE: To investigate the chemical constituents of Fraxinus paxiana. METHOD: The chemical constituents were isolated and purified by chromatographic techniques and the structures of the compounds were identified with or by spectroscopic methods. RESULT: Fifteen compounds were obtained from the m...
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PMID: 19086636
PDF is available here.
PDF is available here.
Neuropharmacology (53)6 2007
Abstract
We examined the effect of esculin, which was extracted from Fraxinus sielboldiana blume, on DA-induced cytotoxicity and the underlying mechanism in human neuroblastoma SH-SY5Y cells. Our results suggest that the protective effects of esculin (10(-7), 10(-6) and 10(-5) M) on DA-induced cytotoxicity m...
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PMID: 17904593
PDF is available here.
PDF is available here.
Abstract
Our study provides the first evidence that purified esculeogenin A significantly suppresses the activity of ACAT protein and leads to reduction of atherogenesis....
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PMID: 17872457
PDF is available here.
PDF is available here.
Abstract
We further investigated the modifying effect of esculin intake on the development of DMH-induced colonic aberrant crypt foci (ACF). Animals were given DMH once a week for 4 weeks to induce ACF. They then received water containing esculin ad libitum for 5 weeks (initiation phase) or 11 weeks after DM...
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PMID: 17978474
PDF is available here.
PDF is available here.
Abstract
Free radicals and reactive oxygen species (ROS), which are generated by UV irradiation, may cause serious injury to skin cell membranes, DNA and functional proteins. In addition, these agents stimulate the expressions of matrix metalloproteinases (MMPs), which can degrade most components of the extr...
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PMID: 18038908
PDF is available here.
PDF is available here.
Abstract
In this paper, a micelle-mediated extraction and cloud point preconcentration method was developed for the determination of less hydrophobic compounds aesculin and aesculetin in Cortex fraxini by HPLC. Non-ionic surfactant oligoethylene glycol monoalkyl ether (Genapol X-080) was employed as the extr...
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PMID: 17499472
PDF is available here.
PDF is available here.
Abstract
The experimental results provide the basis for the ascertainment of extraction process of Qinxiang enteric-coated tablet....
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PMID: 18074857
PDF is available here.
PDF is available here.
Abstract
A bacterial strain, DC-186(T), isolated from home-made compost, was characterized for its phenotypic and phylogenetic properties. The isolate was a Gram-negative rod that was able to grow at 15-36 degrees C and pH 5.5-8.0. Strain DC-186(T) was positive in tests for catalase, oxidase and beta-galacto...
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PMID: 17625189
PDF is available here.
PDF is available here.
Journal of Chromatography A (1148)1 2007
Abstract
A capillary zone electrophoresis (CZE) with indirect laser-induced fluorescence detection (ILIFD) method is described for the simultaneous determination of esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside. The baseline separation was achieved within 5 min with running buffer (p...
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PMID: 17383667
PDF is available here.
PDF is available here.
Abstract
Twelve strains of gram-negative, nonfermenting rods recovered mainly from septicemic patients were studied using conventional and molecular methods. The phenotypic profiles of these strains most closely resembled Psychrobacter phenylpyruvicus. They produced catalase, oxidase, urease, and H(2)S (lead...
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PMID: 17287332
PDF is available here.
PDF is available here.
Phytochemical Analysis (18)3 2007
Abstract
A new bioautographic assay suitable for the localisation of beta-glucosidase inhibitors present in a complex matrix is described. Enzyme activity was detected using esculin as the substrate to produce esculetin, which reacts with ferric ion to form a brown complex.
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PMID: 17500363
PDF is available here.
PDF is available here.
Abstract
Overall, 72 strains of lactic acid thermophilic streptococci isolated from sour milk products manufactured in various regions of Russia and European countries were analyzed using classical microbiological and molecular biological methods. Physiological and biochemical properties and genetic diversit...
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PMID: 18173109
PDF is available here.
PDF is available here.
Journal of Medical Microbiology (55)Pt 11 2006
Abstract
Molecular typing and virulence markers were used to evaluate the genetic profiles and virulence potential of 106 Yersinia enterocolitica strains. Of these strains, 71 were bio-serotype 4/O : 3, isolated from human and animal clinical material, and 35 were of biotype 1A or 2 and of diverse serotypes,...
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PMID: 17030914
PDF is available here.
PDF is available here.
Salicola salis sp. nov., an extremely halophilic bacterium isolated from Ezzemoul sabkha in Algeria.
Abstract
A novel, extremely halophilic bacterium was isolated from brine samples collected from Ezzemoul sabkha in north-east Algeria. Cells of this isolate, designated B2T, were Gram-negative, rod-shaped and motile. Growth occurred between 10 and 25% (w/v) NaCl and the isolate grew optimally at 15-20% (w/v)...
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PMID: 17082405
PDF is available here.
PDF is available here.
Analysis of ultraviolet absorption spectrum of Chinese herbal medicine-Cortex Fraxini by double ANN.
Abstract
A fast, accurate and convenient method for the simultaneous determination of multi-component in the Chinese herbal medicine was proposed by using ultraviolet absorption spectrum. In this method, dummy components were added to training sample, and a double artificial neural network (DANN) that has th...
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PMID: 16914366
PDF is available here.
PDF is available here.
Abstract
The genome of Streptococcus mutans UA159 contains two phospho-beta-glucosidase genes, bglA and celA, which occur in operon-like arrangements along with genes for components of phosphotransferase transport systems and a third phospho-beta-glucosidase encoded by the arb gene, which does not have its o...
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PMID: 16390337
PDF is available here.
PDF is available here.
BMC Microbiology (6)1 2006
Abstract
To localize the promoter activity associated with the bglP locus, a series of transcriptional lacZ gene fusions was formed on a reporter shuttle vector using various DNA fragments from the bglP promoter region. Subsequent beta-galactosidase assays in S. mutans localized the bglP promoter region and...
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PMID: 16630357
PDF is available here.
PDF is available here.
Journal of Chromatography A (1098)1-2 2005
Abstract
Aesculetin is the product of the hydrolysis reaction of aesculin. A high sensitivity and good repeatability method based on capillary electrophoresis with amperometric detection (CE-AD) was developed for simultaneous determination of aesculin and aesculetin in the hydrolysate of aesculin. Under the...
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PMID: 16288763
PDF is available here.
PDF is available here.
Abstract
A non-aqueous capillary electrophoresis method has been developed for the separation and simultaneous determination of fraxin, esculin and esculetin in Cortex fraxini and its preparation for the first time. Optimum separation of the analytes was obtained on a 47 cm x 75 microm i.d. fused-silica capi...
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PMID: 15828063
PDF is available here.
PDF is available here.
Journal of Microbiology (43)5 2005
Abstract
More than seventy strains of aerobic bacteria showing beta-glucosidase activity were isolated from a ginseng field, using a newly designed Esculin-R2A agar, and identified by their 16S rRNA gene sequences. Of these microorganisms, twelve strains could convert the major ginsenoside, Rb(1), to the pha...
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PMID: 16273039
PDF is available here.
PDF is available here.
Abstract
A simple method for the simultaneous determination of five bioactive components (rutin, puerarin, daidzein esculin and esculetin) in traditional medicinal preparations by non-aqueous capillary electrophoresis with UV detection has been developed for the first time. A running buffer composed of 15% a...
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PMID: 15894450
PDF is available here.
PDF is available here.
Abstract
The Universal Coverage Policy (UCP) or "30 Baht Scheme" was launched in Thailand in 2001. The policy caused a cutback in the budgets of all public hospitals and health service centers. Traditional medicine was then viewed as an alternative to save costs. This study examines whether this had any infl...
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PMID: 16295562
PDF is available here.
PDF is available here.
Abstract
Members of the species Agrobacterium ferrugineum were isolated from marine environments. The type strain of this species (= LMG 22047(T) = ATCC 25652(T)) was recently reclassified in the new genus Pseudorhodobacter, in the order 'Rhodobacterales' of the class 'Alphaproteobacteria'. Strain LMG 128 (=...
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PMID: 15879249
PDF is available here.
PDF is available here.
Abstract
Two sporulating bacterial strains designated CECAP06(T) and CECAP16 were isolated from the rhizosphere of the legume Cicer arietinum in Argentina. Almost-complete 16S rRNA gene sequences identified the isolates as a Paenibacillus species. It was most closely related to Paenibacillus cineris LMG 1843...
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PMID: 15879272
PDF is available here.
PDF is available here.
Abstract
The taxonomic position of Enterobacter dissolvens was re-evaluated based on the analysis of the type strain ATCC 23373T and three clinical isolates. The strains were assigned to the genetic cluster of the species by phylogenetic sequence analysis in the frame of a recent population genetic study. Th...
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PMID: 15900966
PDF is available here.
PDF is available here.
Abstract
Four bacterial strains isolated from the human oral cavity, PPPA19, PPPA21(T), PPPA28 and PPPA30, were characterized by determining phenotypic and biochemical features, cellular fatty acid profiles and the phylogenetic position based on 16S rRNA gene sequence analysis. 16S rRNA gene sequence analysi...
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PMID: 15774668
PDF is available here.
PDF is available here.
Abstract
Two mesophilic anaerobic bacterial strains (Z7(T) and Z1) were isolated from waste water sludge of the Xinanzhang paper mill, Beijing, China. The strains were Gram-positive, non-spore-forming and motile. Cells were thin rods (0.2-0.4x4.0-8.0 microm). Growth of the strains was observed at 20-42 degre...
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PMID: 15545467
PDF is available here.
PDF is available here.
Abstract
Two bacterial strains (FA1 and FA2(T)) were isolated from the phyllosphere of a leguminous tree, Acacia caven, in central Argentina. The strains were Gram-negative, strictly aerobic, rod-shaped, motile and formed yellow-pigmented colonies on nutrient agar. The two-primer RAPD patterns of the two str...
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PMID: 15545449
PDF is available here.
PDF is available here.
Abstract
Two bacterial strains, F23T and R22T, have been isolated from hypersaline habitats in Málaga (S. Spain) and Murcia (E. Spain). The novel strains, similar to previously described Idiomarina species, are slightly curved rods, Gram-negative, chemo-organotrophic, strictly aerobic and motile by a single...
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PMID: 15388745
PDF is available here.
PDF is available here.
Free Radical Research (38)8 2004
Abstract
We further investigated the effect of esculin on pancreatic carcinogenesis by the rapid production model induced by augmentation pressure with a choline-deficient diet, ethionine, methionine and BOP. Esculin was given ad libitum as a 0.05% aqueous solution in either the initiation or promotion phase...
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PMID: 15493457
PDF is available here.
PDF is available here.
Journal of Biotechnology (110)3 2004
Abstract
Rutin and esculin were enzymatically acylated with different aliphatic acids as acyl donors (fatty acids, dicarboxylic acids and omega-substituted fatty acids) by an immobilized lipase from Candida antarctica. The effect of the water content and the acyl donors pattern on the flavonoid initial acyla...
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PMID: 15163517
PDF is available here.
PDF is available here.
Abstract
The human pathogen Escherichia coli O157:H7 is thought to be spread by direct or indirect contact with infected animal or human faeces. The present study investigated the effects of the plant coumarin esculin and its aglycone esculetin on the survival of a strain of E. coli O157 under gut conditions...
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PMID: 15137927
PDF is available here.
PDF is available here.
BioFactors (22)1-4 2004
Abstract
These results suggest that the intake of esculin has an inhibitory effect on BOP-induced oxidative DNA damage and carcinogenesis in hamster pancreas....
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PMID: 15630258
PDF is available here.
PDF is available here.
Folia Microbiologica (49)6 2004
Abstract
A group of sixteen esculin-positive fluorescent pseudomonads isolated from an underground brook flowing through a cave complex was characterized by biotyping, multiple enzyme restriction fragment length polymorphism analysis of 16S rDNA (MERFLP), ribotyping and whole-cell fatty-acid methyl-esters an...
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PMID: 15881410
PDF is available here.
PDF is available here.
Abstract
Topical nitroglycerine produces 'chemical sphincterotomy' with reduction in mean anal resting pressure. Pain relief and healing of fissure occurred earlier with GTN than with other treatments. GTN should be considered as the treatment of choice for the non-surgical management of patients with chroni...
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PMID: 15250565
PDF is available here.
PDF is available here.
Abstract
We examined whether coumarins are able to suppress the formation of 8-oxodG in the DNA of human diploid fibroblasts, TIG-7 cells, treated with LOOH and iron(III) ion. Cotreatment of TIG-7 cells with esculetin (6,7-dihydroxycoumarin) significantly suppressed the increase in 8-oxodG content induced by...
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PMID: 12808296
PDF is available here.
PDF is available here.
Abstract
Colony morphology on kanamycin esculin azide agar was investigated as a means of selecting different species and strains of enterococci from clinical specimens. Four representative colonies of each morphotype were indistinguishable by pulsed-field gel electrophoresis, biotype, and antibiogram analys...
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PMID: 12791893
PDF is available here.
PDF is available here.
Microbiology (149)Pt 5 2003
Abstract
An open reading frame (ORF) that would encode a putative antiterminator protein (LicT) of the BglG family was identified in the genomic DNA sequence of Streptococcus mutans. A DNA sequence that would encode a potential ribonucleic antiterminator (RAT) site in the mRNA at which the putative antitermi...
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PMID: 12724394
PDF is available here.
PDF is available here.
Abstract
We re-examined and verified the biochemical characteristics of T. medium. The fermentation pattern of carbohydrates of T. medium resembled that of Treponema vincentii and Treponema denticola, but T. medium was clearly differentiated from T. vincentii in the production of indole, and from T. denticol...
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PMID: 12654104
PDF is available here.
PDF is available here.
Abstract
Seven obligately anaerobic, gram-positive, rod-shaped, spore-forming organisms isolated from human sources were characterized using phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing showed that the strains were genetically highly related to each other (displaying >99%...
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PMID: 12747414
PDF is available here.
PDF is available here.
Planta Medica (68)11 2002
Abstract
By means of HIV gp41 binding affinity directed chromatographic fractionation, three phenylethanoid glycosides; calceolarioside A ( 1), calceolarioside B ( 2) and acteoside ( 3), along with four hydroxycoumarins; esculin ( 4), fraxin ( 5), fraxetin ( 6) and esculetin ( 7), and one lignan, (-)-olivil...
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PMID: 12451497
PDF is available here.
PDF is available here.
Abstract
The present study characterised 73 Hafnia alvei isolates and five Escherichia isolates (originally identified as H. alvei) isolated from cases of diarrhoeal disease by the International Centre for Diarrhoeal Disease Research Branch (ICDDRB) in Bangladesh. Based upon the hydrolysis of arbutin and aes...
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PMID: 12132774
PDF is available here.
PDF is available here.
Abstract
OBJECTIVE: To demonstrate the chemical constituents of Alyxia sinensis. METHOD: The constituents were isolated by column chromatography and identified by advanced physical and spectral analysis. RESULT: Eight compounds have been isolated and elucidated as bauereny acetate(18), scopletin(19), liriode...
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PMID: 12774400
PDF is available here.
PDF is available here.
Planta Medica (68)2 2002
Abstract
The natural product aesculin was demonstrated to possess potent hypouricemic effects in in vivo models of hyperuricemia in both mice and rats pretreated with oxonate. Aesculin, when administered intraperitoneally to the oxonate-induced hyperuricemic rodents, was able to elicit dose-dependent hypouri...
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PMID: 11859475
PDF is available here.
PDF is available here.
Abstract
A beta-glucoside utilization regulon recently isolated from Streptococcus mutans has been shown to contain genes involved in beta-glucoside hydrolysis and a putative regulator. The bglP gene encodes a beta-glucoside-specific enzyme II (EII) component of the phosphoenolpyruvate-dependent phosphotrans...
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PMID: 11860549
PDF is available here.
PDF is available here.
Abstract
Our studies establish that measurement of the effect of inhibitors on presteady state currents is a valid non-radioactive method for the determination of inhibitor binding constants. Furthermore, analysis of the presteady state currents provide novel insights into partial reactions of the transport...
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PMID: 11588102
PDF is available here.
PDF is available here.
Abstract
We investigated suicidal strains of Aeromonas species as means of understanding animal virulence and enteropathogenicity. Non suicidal strains of A. Hydrophila showed and overall 88.8% lethality rate and non suicidal strains of A. veronii biotype sobria showed 83.3% lethality rate and was nil for it...
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PMID: 12035354
PDF is available here.
PDF is available here.
Abstract
A new medium is described for the isolation of Listeria spp. from foods and environmental samples. It is based on a modified Oxford medium in which 3,4-cyclohexenoesculetin-beta-D-glucoside replaces aesculin. Positive colonies are intensely black with the advantage that the pigment does not diffuse...
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PMID: 11169047
PDF is available here.
PDF is available here.
Abstract
Clinical features and functional activity of T- and B-cells were studied on the model of adjuvant arthritis which is the disease associated with activation of B-cells after monotherapy of rats with IM-1 (B-cell stimulator), dibazol (T-cell stimulator), alpha-tocopherol acetate (cintrol). It was esta...
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PMID: 11565289
PDF is available here.
PDF is available here.
Abstract
Eighteen strains isolated from human specimens or from food products were characterized as atypical variants of mannitol-negative Streptococcus bovis. They were tested for extended biochemical criteria, ribotyping and DNA-DNA hybridization in order to define their taxonomic status. These strains wer...
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PMID: 10939646
PDF is available here.
PDF is available here.
Microbiology (146 ( Pt 7)) 2000
Abstract
A regulon from Streptococcus mutans that plays a role in the utilization of beta-glucosides has been isolated, sequenced and subjected to sequence analysis. This regulon encodes a beta-glucoside-specific Enzyme II (EII) component (bglP) of the phosphoenolpyruvate-dependent phosphotransferase system...
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PMID: 10878120
PDF is available here.
PDF is available here.
Abstract
Outbreaks of foodborne listeriosis caused by Listeria monocytogenes in recent years, and the high mortality rate associated with listeriosis, have raised the need for reliable and rapid detection of the pathogen. A simple, automated method was developed for the detection of Listeria organisms in foo...
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PMID: 10889308
PDF is available here.
PDF is available here.
Abstract
Many food and meat processors test environmental swabs and sponges to confirm the absence of Listeria spp. Spectral pattern changes in a liquid growth medium, resulting from esculin hydrolysis by Listeria in contaminated swabs and sponges, were automatically monitored by the BioSys instrument in a s...
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PMID: 10857550
PDF is available here.
PDF is available here.
Abstract
A simple anaerobic recovery-enrichment system, semisolid Penn State University (ssPSU) broth, that enhances recovery of heat-injured Listeria monocytogenes, was rapidly achieved in 10-ml screw-capped tubes by adding Bacto-agar (2.5 g/liter) and L-cysteine (0.5 g/liter) to Penn State University broth...
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PMID: 10772211
PDF is available here.
PDF is available here.
Abstract
Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic] / Ministerstvo meditsinskoi i mikrobiologicheskoi promyshlennosti SSSR (45)8 2000
[Antibiotics sensitivity and characteristics of the esculin-positive Pseudomonas aeruginosa biovar].
Abstract
Strains of Pseudomonas aeruginosa hydrolyzing esculin were isolated for the first time. They amount to 17.1 +/- 2.0% (60 from 325) of the investigated P. aeruginosa strains isolated from the clinical material in St. Petersburg. Esculin hydrolysis was measured by micromethod in plates, results were a...
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PMID: 10989719
PDF is available here.
PDF is available here.