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Sugar Phosphates (8):
Recent article
Sugar Phosphates
Journal of Antibiotics (63)10 2010
Abstract
We found that a herbicide, ketoclomazone, exhibited antibacterial activity against a pathogenic bacterium, Haemophilus influenzae, with an MIC value of 12.5 μg ml(-1) and that antibacterial activity was suppressed by adding 1-deoxyxylulose, a free alcohol of 1-deoxy-D-xylulose 5-phosphate (DXP...
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PMID: 20808315
PDF is available here.
PDF is available here.
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Eukaryotic Cell (9)7 2010
Abstract
We characterized the catalytic glucokinase and hexokinase from the opportunistic human pathogen Aspergillus fumigatus and showed that both enzymes display different biochemical properties and play different roles during growth and development. Glucokinase efficiently activates glucose and mannose bu...
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PMID: 20453072
PDF is available here.
PDF is available here.
Plant Physiology (153)3 2010
Abstract
We investigated intraspecific differences of volatile emissions induced from rosette leaves of 27 accessions of Arabidopsis (Arabidopsis thaliana) upon treatment with coronalon, a jasmonate mimic eliciting responses similar to those caused by insect feeding. Quantitative variation was found for the...
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PMID: 20463089
PDF is available here.
PDF is available here.
Phytochemistry (71)8-9 2010
Abstract
We developed a sensitive assay suitable for plant extracts that is based on the decarboxylation of labeled pyruvate (1-(13)C)-PYR and detection of (13)CO(2) by isotope ratio mass spectrometry. We tested our method investigating the DXS activity in poplar leaves. Apparent DXS activity showed Michaeli...
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PMID: 20303132
PDF is available here.
PDF is available here.
Mediators of Inflammation (2010) 2010
Abstract
Despite a decrease in mortality over the last decade, sepsis remains the tenth leading causes of death in western countries and one of the most common cause of death in intensive care units. The recent discovery of Toll-like receptors and their downstream signalling pathways allowed us to better und...
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PMID: 20396414
PDF is available here.
PDF is available here.
Abstract
Sedoheptulose-7-phosphate isomerase (GmhA) converts d-sedoheptulose 7-phosphate to d,d-heptose 7-phosphate. This is the first step in the biosynthesis pathway of NDP-heptose, which is responsible for the pleiotropic phenotype. This biosynthesis pathway is the target of inhibitors to increase the mem...
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PMID: 19923728
PDF is available here.
PDF is available here.
Abstract
We report incorporation of either a heterologous mevalonate pathway (MEV) or enhancement of the endogenous methyl erythritol phosphate pathway (MEP) with our modular metabolic engineering system. With MEP pathway enhancement, it was found that pyruvate supplementation of rich media and simultaneous...
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PMID: 19777230
PDF is available here.
PDF is available here.
Abstract
We present an analysis of the chemical function of a recombinant bifunctional phosphomannose isomerase/GDP-mannose pyrophosphorylase (manC) from Pyrococcus furiosus DSM 3638 and its use in the synthesis of guanidinediphospho-hexoses and a range of nucleotidediphospho-mannoses. This enzyme is unusual...
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PMID: 19421452
PDF is available here.
PDF is available here.
Abstract
We showed that expression of the aiiB gene was controlled by agrocinopines A and B and required the agrocinopine-ABC transporter Acc, but was not affected by the level of quorum-sensing (QS) signal 3-oxo-octanoylhomoserine lactone (OC8-HSL). In the presence of agrocinopines, a constructed aiiB mutan...
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PMID: 19348571
PDF is available here.
PDF is available here.
Abstract
1-Deoxy-D-xylulose-5-phosphate synthase (DXS) catalyses the first committed step of the 2C-methyl-D-erythritol-4-phosphate (MEP) pathway, which is an alternative isoprenoids biosynthetic route that has been recently discovered. In this work, a DXS1-like cDNA (GmDXS1) was isolated from soybean. The f...
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PMID: 18437529
PDF is available here.
PDF is available here.
Abstract
A stream of genetic and biochemical information available for the biosynthesis of aminocyclitols over the past few years has provided the foundation to study the modes of formation of this clinically important class of natural products. In addition to work on the identification and functional analys...
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PMID: 19321377
PDF is available here.
PDF is available here.
Current Issues in Molecular Biology (11 Suppl 1) 2009
Abstract
We decided to compile the data on cloned and characterized genes of MEP pathway in plants. Also, we summarize the results of the previously published reports, particularly those which were based on incorporation of 13C-glucose or by application of specific inhibitors such as mevinolin and fosmidomyc...
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PMID: 19193963
PDF is available here.
PDF is available here.
Abstract
To work out a test system for studying the inhibition of isoprenoid biosynthesis through non-mevalonate pathway, the conversion of 2-(14)C-methylerythritol 2,4-cyclodiphosphate (14C-MEC) into isoprenoids of three bacteria species and into plastids of 11 higher plant species was studied. The highest...
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PMID: 19845288
PDF is available here.
PDF is available here.
Abstract
We propose a model wherein GcpE turnover represents a bottleneck of the MEP pathway in plant leaves simultaneously exposed to high irradiance and hot temperature....
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PMID: 19021881
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (289)1 2008
Abstract
We show that cg2927 (scrB) encodes sucrose-6-phosphate hydrolase. The purified His-tagged protein hydrolyzed sucrose-6-phosphate and sucrose, but not sucrose-6'-phosphate. The Km value for sucrose was 190 mM while the Km for sucrose-6-phosphate was much lower, 0.04 mM. Sucrose-6-phosphate hydrolase...
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PMID: 19054097
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)24 2008
Abstract
We detected genes of four different pathways for trehalose synthesis in the genome of this organism, namely, the trehalose-6-phosphate synthase (Tps)/trehalose-6-phosphate phosphatase (Tpp), TreS, TreY/TreZ, and TreT pathways. Moreover, R. xylanophilus is the only known member of the phylum Actinoba...
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PMID: 18835983
PDF is available here.
PDF is available here.
Infection and Immunity (76)11 2008
Abstract
We show that heterologous expression of gcpE and lytB in L. innocua can functionally restore the MEP pathway in this organism and confer on it the ability to induce Vgamma9 Vdelta2 T cells. We have previously confirmed that both pathways are functional in L. monocytogenes and can provide sufficient...
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PMID: 18765739
PDF is available here.
PDF is available here.
Analytica Chimica Acta (627)2 2008
Abstract
The solution-phase sensor array of three cationic bis-boronic acid appended benzyl viologens (BBV) and the anionic fluorescent dye, 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS), is able to discriminate among five phospho sugars, four nucleotides and three neutral saccharides in aqueo...
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PMID: 18809074
PDF is available here.
PDF is available here.
Plant Journal (56)1 2008
Abstract
The first step of the plastidial methylerythritol phosphate (MEP) pathway is catalyzed by two isoforms of 1-deoxy-D-xylulose 5-phosphate synthase (DXS1 and DXS2). In Medicago truncatula, MtDXS1 and MtDXS2 genes exhibit completely different expression patterns. Most prominently, colonization by arbus...
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PMID: 18557838
PDF is available here.
PDF is available here.
Biochemical Journal (415)1 2008
Abstract
TAL (transaldolase) was originally described in the yeast as an enzyme of the PPP (pentose phosphate pathway). However, certain organisms and mammalian tissues lack TAL, and the overall reason for its existence is unclear. Recently, deletion of Ser(171) (TALDeltaS171) was found in five patients caus...
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PMID: 18498245
PDF is available here.
PDF is available here.
Plant Journal (55)6 2008
Abstract
SUMMARY: A sensitive and robust mixed-mode high performance liquid chromatography-tandem mass spectrometry method was developed for the qualitative and quantitative determination of sugar phosphates, which are notoriously difficult to separate using reversed-phase materials. Sugar phosphates were se...
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PMID: 18494852
PDF is available here.
PDF is available here.
Biochemistry (Washington) (47)31 2008
Abstract
We searched for genes capable of restoring growth of a TIM-deficient bacterium under gluconeogenic conditions. Rather than discovering an isomerase, we selected yghZ, a gene encoding a member of the aldo-keto reductase superfamily. Here we show that YghZ catalyzes the stereospecific, NADPH-dependent...
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PMID: 18620424
PDF is available here.
PDF is available here.
Carbohydrate Research (343)10-11 2008
Abstract
The synthesis of dibenzyl 6-O-naphthylmethyl-2,3,5-tri-O-benzoyl-beta-D-galactofuranosyl-(1-->5)-2,3-di-O-benzoyl-6-O-benzyl-beta-D-galactofuranosyl-(1-->4)-3-O-benzyl-2-O-pivaloyl-alpha-l-rhamnopyranosyl-(1-->3)-2-acetamido-2-deoxy-4,6-di-O-benzoyl-alpha-D-glucopyranosyl phosphate (1), a protected...
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PMID: 18513706
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)30 2008
Abstract
These results were further verified by incorporation of [5-(2)H]mevalonate or [5,5-(2)H(2)]deoxyxylulose into dolichols as well as by the observed decreased accumulation of dolichols upon treatment with mevinolin or fosmidomycin, selective inhibitors of either pathway. The presented data indicate th...
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PMID: 18502754
PDF is available here.
PDF is available here.
Abstract
We investigated whether upregulated levels of hydroxymethylbutenyl diphosphate synthase (HDS) could increase the metabolic flux through this pathway, as judged by endpoint (carotenoid) measurements. Unlike other MEP pathway enzymes, however, increasing the levels of an active HDS protein in caroteno...
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PMID: 18452711
PDF is available here.
PDF is available here.
Analytical Biochemistry (377)2 2008
Abstract
A systematic comparison of six sugar indicators for their sensitivity, specificity, cross-reactivity, and suitability in the context of crude lysates revealed para-hydroxybenzoic acid hydrazide (pHBH) to be best suited for application in a plate-based phosphatase-assisted universal sugar-1-phosphate...
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PMID: 18387352
PDF is available here.
PDF is available here.
Abstract
We searched for P. marinus methylerythritol phosphate (MEP) pathway genes, responsible for de novo isoprenoid synthesis in plastids, and determined the full-length gene sequences for 6 of 7 of these genes. Phylogenetic analyses revealed that each P. marinus gene clusters with orthologs from plastid-...
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PMID: 18359776
PDF is available here.
PDF is available here.
Bioorganic Chemistry (36)3 2008
Abstract
The genes xpk1 and xpk2(Delta1-21) encoding phosphoketolase-1 and (Delta1-7)-truncated phosphoketolase-2 have been cloned from Lactobacillus plantarum and expressed in Escherichia coli. Both gene-products display phosphoketolase activity on fructose-6-phosphate in extracts. A N-terminal His-tag cons...
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PMID: 18430452
PDF is available here.
PDF is available here.
Biochemical Journal (412)1 2008
Abstract
T6P (trehalose 6-phosphate), the precursor of trehalose, has come out of obscurity over 10 years to be appreciated as an important regulator of plant metabolism and development, quite possibly linking the two. This information has been gained from analysis of mutant and transgenic plants, which show...
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PMID: 18426388
PDF is available here.
PDF is available here.
Plant Cell Online (20)5 2008
Abstract
We have identified an Arabidopsis thaliana mutant, rif1, in which posttranscriptional upregulation of MEP pathway enzyme levels is caused by the loss of function of At3g47450, a gene originally reported to encode a mitochondrial protein related to nitric oxide synthesis. However, we show that nitric...
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PMID: 18469163
PDF is available here.
PDF is available here.
Plant Journal (54)3 2008
Abstract
We utilized a proteomics-based approach with isolated trichomes of four different sweet basil (Ocimum basilicum L.) lines possessing very different metabolite profiles to clarify the regulation of metabolism in this single cell type. Significant differences in the distribution and accumulation of th...
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PMID: 18248593
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)10 2008
Abstract
We found that the expression of the acc operon is induced by agrocinopines and also by limitation of phosphate. The main promoter is present in front of the first gene, accR, which codes for a repressor. This operon structure enables efficient repression when opine levels are low. The promoter conta...
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PMID: 18344359
PDF is available here.
PDF is available here.
Carbohydrate Research (343)5 2008
Abstract
As Leloir glycosyltransferases are increasingly being used to prepare oligosaccharides, glycoconjugates, and glycosylated natural products, efficient access to stereopure sugar nucleotide donor substrates is required. Herein, the rapid synthesis and purification of eight sugar nucleotides is describ...
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PMID: 18299123
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (281)1 2008
Abstract
The central carbon metabolism is well investigated in bacteria, but this is not the case for archaea. MJ0400-His(6) from Methanocaldococcus jannaschii catalyzes the cleavage of fructose-1,6-bisphosphate (FBP) to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate with a V(max) of 33 mU mg(-1)...
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PMID: 18318840
PDF is available here.
PDF is available here.
Plant Molecular Biology (66)6 2008
Abstract
We used green fluorescent fusion protein assays to demonstrate that the Arabidopsis MEP pathway enzymes are localized to the chloroplast. We have also characterized three Arabidopsis albino mutants, ispD-1, ispD-2 and ispE-1, which have T-DNA insertions in the IspD and IspE genes of the MEP pathway....
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PMID: 18236010
PDF is available here.
PDF is available here.
Abstract
MurNAc etherases cleave the unique D-lactyl ether bond of the bacterial cell wall sugar N-acetylmuramic acid (MurNAc). Members of this newly discovered family of enzymes are widely distributed among bacteria and are required to utilize peptidoglycan fragments obtained either from the environment or...
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PMID: 18049859
PDF is available here.
PDF is available here.
Abstract
We report the first unambiguous syntheses of glucitol-1,6-bis-phosphate and mannitol-1,6-bis-phosphate and their competitive inhibition of various fructose bis-phosphate aldolases.
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PMID: 18261903
PDF is available here.
PDF is available here.
Plant Molecular Biology (66)5 2008
Abstract
We investigated the cellular effects of depletion of IspE, a protein involved in the MEP pathway, using virus-induced gene silencing (VIGS). The IspE gene is preferentially expressed in young tissues, and induced by light and methyl jasmonate. The GFP fusion protein of IspE was targeted to chloropla...
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PMID: 18180879
PDF is available here.
PDF is available here.
Abstract
Structure-based design of alkyl sugar-1-phosphates provides an efficient nucleotidylyltransferase-catalyzed synthesis of a series of new lipophilic sugar nucleotides possessing long or branched alkyl chains, thereby demonstrating the utility of nucleotidylyltransferases to catalyze the synthesis of...
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PMID: 18219417
PDF is available here.
PDF is available here.
Abstract
We report structures of GmhA from Escherichia coli and Pseudomonas aeruginosa in apo, substrate, and product-bound forms, which together suggest that GmhA adopts two distinct conformations during isomerization through reorganization of quaternary structure. Biochemical characterization of GmhA mutan...
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PMID: 18056714
PDF is available here.
PDF is available here.
Abstract
Pharmaceutical Research (25)1 2008
Abstract
These findings suggest that plasma pharmacokinetics and activity of eritoran maybe influenced by eritoran micelle size and plasma TC and TG concentrations....
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PMID: 17849177
PDF is available here.
PDF is available here.
Abstract
For the RNA world hypothesis to be accepted, the constitutional self-assembly of RNA will have to be demonstrated. Conceptually, the simplest route to RNA involves nucleotide polymerisation. Activated pyrimidine nucleotides can be derived from arabinose-3-phosphate under potentially prebiotic condit...
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PMID: 18203227
PDF is available here.
PDF is available here.
Journal of Organic Chemistry (72)26 2007
Abstract
Efficient syntheses of the non-mevalonate pathway intermediates 2-C-methylerythritol 4-phosphate (MEP) and 2-C-methylerythritol 2,4-cyclodiphosphate (ME-2,4-cycloPP), as well as the parent tetrol 2-C-methylerythritol, in enantiopure form from (2S,4R)-cis-2-phenyl-4-tert-butyldimethylsilyloxy-1,3-dio...
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PMID: 18020362
PDF is available here.
PDF is available here.
Carbohydrate Research (342)17 2007
Abstract
Starting from l-lyxose, indium-mediated chain elongation with allyl bromide followed by acetylation and oxidative cleavage of the double bond and deprotection afforded 2-deoxy-l-galacto-heptose as a 2-deoxy analogue of the bacterial carbohydrate l-glycero-d-manno-heptose in good overall yield. For t...
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PMID: 17880933
PDF is available here.
PDF is available here.
Physical Review Letters (99)22 2007
Abstract
Partial density of states in the empty conduction band of the phosphate backbone sites in DNA was probed using energy-dependent resonant Auger spectroscopy. Results show that genomic DNA with periodic backbones exhibits an extended state despite separation of each phosphate group by an insulating su...
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PMID: 18233331
PDF is available here.
PDF is available here.
Journal of Bacteriology (189)24 2007
Abstract
We demonstrate that Rv3582c is essential for M. tuberculosis: Rv3582c has been cloned and expressed, and the encoded protein has been purified. The purified M. tuberculosis IspD protein was capable of catalyzing the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol in the presence of MEP and C...
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PMID: 17921290
PDF is available here.
PDF is available here.
Journal of Chromatography A (1172)2 2007
Abstract
We have demonstrated the utility of our method for the analysis of biological samples by applying it to the simultaneous quantitation of changes in soluble sugars and sugar phosphates in A. thaliana Columbia-0 (Col-0) and its starchless phosphoglucomutase (pgm) mutant over a 12-h light/12-h dark gro...
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PMID: 17961583
PDF is available here.
PDF is available here.
Biochemistry (Washington) (46)44 2007
Abstract
We show by surface plasmon resonance (SPR) analyses of constructs containing single amino acid substitutions that these conserved residues (Gln-644, Arg-687, Glu-709, Tyr-714) are critical for carbohydrate recognition by domain 5. Furthermore, the N-glycosylation site at position 711 of domain 5, wh...
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PMID: 17927214
PDF is available here.
PDF is available here.
Abstract
LPS upregulates cytokine expression via TLR4 and induces attenuation of smooth muscle contractility which can be effectively antagonized....
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PMID: 17656666
PDF is available here.
PDF is available here.
Abstract
We have previously found increased amounts of a seven-carbon chain carbohydrate which we suspected of being sedoheptulose. We report development of a liquid chromatography-tandem mass spectrometry method for quantitation of the seven-carbon carbohydrates sedoheptulose and mannoheptulose in urine. Ad...
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PMID: 17603756
PDF is available here.
PDF is available here.
Abstract
Toll-like receptors (TLRs) are membrane-bound sensors that detect and respond to microbial infection. Two studies in Cell, one in this issue, reveal how TLRs recognize their ligands. Kim et al. (2007) recently reported the structure of TLR4 bound to the accessory protein MD-2 and its antagonist, the...
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PMID: 17889640
PDF is available here.
PDF is available here.
Journal of Chromatography A (1164)1-2 2007
Abstract
We have developed an improved analytical method for the determination of sugar phosphates using sodium carbonate (Na(2)CO(3)) for high-performance anion-exchange chromatography-pulsed amperometric detection. The target analytes were separated completely within 10 min using eluent containing 20 mM Na...
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PMID: 17658539
PDF is available here.
PDF is available here.
Abstract
We determined the structure of the full-length ectodomain of the mouse TLR4 and MD-2 complex. We also produced a series of hybrids of human TLR4 and hagfish VLR and determined their structures with and without bound MD-2 and Eritoran. TLR4 is an atypical member of the LRR family and is composed of N...
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PMID: 17803912
PDF is available here.
PDF is available here.
Plant Molecular Biology (65)1-2 2007
Abstract
We have cloned a cDNA encoding hydroxymethylbutenyl diphosphate synthase (HDS), a MEP pathway enzyme, and generated antibodies to investigate the distribution of transcripts and protein in MIA-producing aerial tissues. Consistent with our earlier work, transcripts for the genes encoding the so-calle...
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PMID: 17611800
PDF is available here.
PDF is available here.
Plant Molecular Biology (65)1-2 2007
Abstract
We have previously shown that the production of diterpene hydrocarbons leading to phytoalexins and the expression of related biosynthetic genes are activated in suspension-cultured rice cells upon elicitor treatment. To better understand the elicitor-induced activation of phytoalexin biosynthesis, w...
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PMID: 17634747
PDF is available here.
PDF is available here.
Abstract
We have previously reported the discovery of potent and selective inhibitors of 6-phosphogluconate dehydrogenase, the third enzyme of the phosphate pentose pathway, from Trypanosoma brucei, the causative organism of human African trypanosomiasis. These inhibitors were charged phosphate derivatives w...
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PMID: 17615587
PDF is available here.
PDF is available here.
Phytochemistry (68)15 2007
Abstract
The biosynthesis of (2S)-2-methyl-2-(4'-methyl-3'-pentenyl)-8-(3''-methyl-2-butenyl)-2H-1-benzopyran-6-carboxylic acid (gaudichaudianic acid), the major metabolite in leaves and roots of Piper gaudichaudianum Kunth (Piperaceae), has been investigated employing [1-(13)C]-D-glucose as precursor. The l...
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PMID: 17574633
PDF is available here.
PDF is available here.
Abstract
We investigated the distribution of genes for both pathways in the green alga Mesostigma viride, a key species at the basis of streptophycean (charophycean green algae, land plant) evolution. Ten of altogether twelve generally weakly expressed genes for isoprenoid biosynthesis, including three for t...
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PMID: 17433859
PDF is available here.
PDF is available here.
Abstract
We here report a fast and reliable quantitative method based on the use of liquid nitrogen, a weak acid extraction, and liquid chromatography-electrospray ionization tandem mass spectrometry to quantify simultaneously the intracellular concentration of sugar phosphate pools. The method was set up us...
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PMID: 17523595
PDF is available here.
PDF is available here.