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Cells (32)
Articles on Cells
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Microtubule Dynamics at the Cell Cortex Probed by TIRF Microscopy
Methods Cell Biol (2010) PMID 20719267
We describe the application of a commercially available setup to analyze microtubule behavior in live mammalian cells using TIRF microscopy. 2010 Elsevier Inc. All rights reserved.... -
Far-field superposition method for three-dimensional computation of light scattering from multiple cells.
J Biomed Opt 15(5):055006 (2010) PMID 21054088
A linear coherent superposition method for estimating the plane wave far-field scattering pattern from multiple biological cells computed by the finite-difference time-domain (FDTD) method is presented. The method enables the FDTD simulation results of scattering from a small number... -
Is a single cell sensor possible?
Conf Proc IEEE Eng Med Biol Soc (2010) PMID 21096867
A cell-based implant is a miniaturized sensor that can be placed inside a biological living cell. This device would be able to interrogate and possibly affect biological functions in vivo. This paper explores the requirements of such a system; it also investigates the changes that ne... -
Microtubule Dynamics in Plant Cells
Methods Cell Biol (2010) PMID 20719281
We describe a biochamber that allows for plant cell growth and development while allowing gas exchange and reducing evaporation. Another major difficulty is the limited fluorescence lifetime and we describe imaging strategies to reduce photobleaching in long-term imaging. We also discuss methods of... -
Isolating Tubulin from Nonneural Sources
Methods Cell Biol (2010) PMID 20466127
We describe protocols for this procedure and describe its application to a number of vertebrate, fungal, protozoal, and plant sources.... -
Drug targeting: learning from toxin entry and trafficking in mammalian cells.
Curr Opin Drug Discov Devel 13(1):86-95 (2010) PMID 20047149
A significant number of therapeutic targets reside inside cells and intracellular organelles. Therapeutics therefore must be able to gain access to cellular compartments, and be able to interact specifically with a given molecule to exert a desired effect. Many naturally occurring toxins perform suc... -
Methods in tubulin proteomics.
Methods Cell Biol (2010) PMID 20466132
We present protocols that we have used for the analysis of tubulin isotypes and PTMs present in tubulin isolated from cells in culture or tissues and for the identification of tubulin regions altered by microtubule-stabilizing agents. Tubulin proteomics complements structural and computer modeling i... -
[Messages from necrotic cells mediate linkage between inflammation and coagulation].
Rinsho Ketsueki 51(1):29-36 (2010) PMID 20134137
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Calculation of cellular oxygen concentration for photodynamic therapy in vitro.
Methods Mol Biol (2010) PMID 20552349
In vitro photodynamic therapy experiments are usually performed by irradiating cells in confluent or nearly confluent monolayer cultures. Oxygen is consumed in the monolayer by photodynamic reactions and cellular respiration and is supplied by diffusion from the overlying medium. Calculations of oxy... -
Photoactivatable Green Fluorescent Protein-Tubulin
Methods Cell Biol (2010) PMID 20719266
We have tagged tubulin with a photoactivatable variant of green fluorescent protein (PA-GFP), thereby allowing one to follow the behavior of a subset of tagged molecules in the cell. Here, we describe methods to tag and express proteins with PA-GFP, locally photoactivate the recombinant protein and...
