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Cell Line, Transformed (1):
Recent article
Cell Line, Transformed
Abstract
We show that ITC-induced apoptosis of oncogene-transformed cells involves thiol modification of DNA topoisomerase II (Top2) based on the following observations. 1) siRNA-mediated knockdown of Top2α in both SV40-transformed MEFs and Ras-transformed human mammary epithelial MCF-10A cells resulted in...
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PMID: 21828038
PDF is available here.
PDF is available here.
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FEBS Journal (278)18 2011
Abstract
We report data showing that TGF-β1 signals via IRS2 in kidney epithelial cells. Small interfering RNA (siRNA)-mediated targeting of IRS2 increased E-cadherin expression, although it did not alter TGF-β1-mediated E-cadherin repression. Phosphorylation of the downstream target of IRS2/Akt signalling...
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PMID: 21777391
PDF is available here.
PDF is available here.
FEBS Journal (278)18 2011
Abstract
We report data showing that TGF-β1 signals via IRS2 in kidney epithelial cells. Small interfering RNA (siRNA)-mediated targeting of IRS2 increased E-cadherin expression, although it did not alter TGF-β1-mediated E-cadherin repression. Phosphorylation of the downstream target of IRS2/Akt signalling...
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PMID: 21777391
PDF is available here.
PDF is available here.
FEBS Journal (278)18 2011
Abstract
We report data showing that TGF-β1 signals via IRS2 in kidney epithelial cells. Small interfering RNA (siRNA)-mediated targeting of IRS2 increased E-cadherin expression, although it did not alter TGF-β1-mediated E-cadherin repression. Phosphorylation of the downstream target of IRS2/Akt signalling...
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PMID: 21777391
PDF is available here.
PDF is available here.
FEBS Journal (278)18 2011
Abstract
We report data showing that TGF-β1 signals via IRS2 in kidney epithelial cells. Small interfering RNA (siRNA)-mediated targeting of IRS2 increased E-cadherin expression, although it did not alter TGF-β1-mediated E-cadherin repression. Phosphorylation of the downstream target of IRS2/Akt signalling...
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PMID: 21777391
PDF is available here.
PDF is available here.
FEBS Journal (278)18 2011
Abstract
We report data showing that TGF-β1 signals via IRS2 in kidney epithelial cells. Small interfering RNA (siRNA)-mediated targeting of IRS2 increased E-cadherin expression, although it did not alter TGF-β1-mediated E-cadherin repression. Phosphorylation of the downstream target of IRS2/Akt signalling...
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PMID: 21777391
PDF is available here.
PDF is available here.
Abstract
We address this challenge, developing a new methodology for analysing and quantifying image data and its integration with biophysical models. Specifically we fit a binding kinetics model to 2 colour fluorescence data for cytoskeleton independent synapses (2 and 3D) and test whether the observed inve...
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PMID: 21829338
PDF is available here.
PDF is available here.
Abstract
Leukaemia inhibitory factor (LIF) is one of the cytokines that is indispensable for embryo implantation. The aim of the present study was to investigate the role of activation of extracellular signal-regulated kinase (ERK) 1/2 in LIF-mediated proliferation of HTR-8/SVneo cells. Stimulation of HTR-8/S...
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PMID: 21635820
PDF is available here.
PDF is available here.
Abstract
We examined the effect of lonicerin, a flavonoid isolated from Lonicerae Flos, on an arthritis caused by C. albicans cell wall (CACW) in mice. To examine the effect, an emulsified mixture of CACW and complete Freund's adjuvant (CACW/CFA) was injected into BALB/c mice via hind footpad route on days -...
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PMID: 21656372
PDF is available here.
PDF is available here.
Abstract
We are developing a bioactive coating based upon a magnetoelastic (ME) material that vibrates in response to an ac magnetic field. In order to establish these coatings for this purpose, the ME material was first rendered bioactive through the sequential addition of polyurethane and chitosan thin fil...
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PMID: 21095859
PDF is available here.
PDF is available here.
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (28)1 2011
Abstract
To establish immortalized lymphoblastoid cell lines of a Miao core pedigree with Bardet-Biedl syndrome (BBS), in order to provide a long-term source of material for research.
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PMID: 21287506
PDF is available here.
PDF is available here.
Abstract
I in Dulbecco's modified Eagle's medium containing 5.5 mM glucose. For cocultures, pericytes and endothelial cells were seeded together on rat tail collagen type I-coated cell culture plates. After 24 h of initial culture, the medium was replaced with serum-free medium containing 0-100 mM g...
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PMID: 21091050
PDF is available here.
PDF is available here.
Abstract
We also analyzed its effect on cell proliferation IN VITRO, cytotoxicity, and skin irritation. Different extracts obtained from the aerial parts of A. pichinchensis, topically administrated, were evaluated in a healing model by scalpel-blade incision on the rat. The extracts, at 10 % concentrations,...
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PMID: 21267810
PDF is available here.
PDF is available here.
Cancer Biology & Therapy (11)2 2011
Abstract
We determined the feasibility of using the ChemoFx DRM to test tumor samples for sensitivity to cetuximab. We exposed four non-small cell lung carcinoma (NSCLC) cell lines (H358, H520, HCC827, and H1666) to cetuximab and determined their sensitivity using the ChemoFx DRM and, in parallel, EGFR statu...
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PMID: 20980824
PDF is available here.
PDF is available here.
Abstract
These results indicate that FFPE samples may retain viral as well as cellular RNA expression information at detectable level. To our knowledge, this is first study which represents elevated expressional levels of EBER1, EBER2 and IL-10 in FFPE tissue samples of Burkitt's lymphoma in Pakistan. These...
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PMID: 21791113
PDF is available here.
PDF is available here.
Abstract
We show that human bronchial epithelial cells (HBECs) display characteristics of multipotent stem cells of the lung. These HBECs express markers indicative of several epithelial types of the adult lung when experimentally tested in cell culture. When cultured in three different three-dimensional (3D...
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PMID: 21760947
PDF is available here.
PDF is available here.
Acta Virologica (55)1 2011
Abstract
We report the construction of plasmid containing the HIV-1 genome mutated in the pol gene, which was co-transfected with plasmids expressing the pol gene products reverse transcriptase (RT) and integrase (IN), and the glycoprotein G of vesicular stomatitis virus (VSV-G). The virions produced in HEK...
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PMID: 21434701
PDF is available here.
PDF is available here.
Abstract
We present a method for the analysis of protein trafficking and shuttling activity between primary and transformed mammalian cells. This method combines the generation of heterokaryon fusions with fluorescence microscopy to provide a flexible protocol that can be used to detect steady-state or dynam...
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PMID: 21445034
PDF is available here.
PDF is available here.
Clinical Cancer Research (17)3 2011
Abstract
These findings suggest that FOXO1 is a direct target of progestin, implicating novel molecular mechanisms of progestin to eradicate endometrial neoplasia....
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PMID: 21131554
PDF is available here.
PDF is available here.
Abstract
We demonstrate that Aurora-A overexpression enhances both the expression level and transcriptional activity of c-Myc. The inhibition of c-Myc expression by RNA interference significantly impaired the oncogenic potential of Aurora-A, resulting in attenuated cellular proliferation and transformation r...
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PMID: 20890087
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)45 2010
Abstract
We found that plasma membrane targeting to mimic persistent Ras activation enhanced the growth-transforming activities of RalGEFs. Unexpectedly, transforming activity did not correlate directly with total cell steady-state levels of Ral activation. Next, we observed elevated Rgl2 expression in PDAC...
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PMID: 20801877
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)45 2010
Abstract
We have now investigated the possible involvement of PiT1 in TNF-induced apoptosis. We show that PiT1-depleted cells are more sensitive to the proapoptotic activity of TNF (i.e. when the antiapoptotic NFκB pathway is inactivated). These observations were made in the human HeLa cancer cell line eith...
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PMID: 20817733
PDF is available here.
PDF is available here.
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology (18)6 2010
Abstract
The aim of this study was to compare cell proliferation and function of the T cells acquired under various culture conditions for establishing a simple, safe and efficient cell expansion protocol in vitro. The peripheral blood mononuclear cells (PBMNC) were isolated and stimulated with autologous den...
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PMID: 21176376
PDF is available here.
PDF is available here.
Abstract
We present results on the morphology of poly(L-lactide-co-epsilon-caprolactone) copolymer micromachined by femtosecond laser and demonstrate the attachment and alignment of C2C12 myoblast cells in the microchannels. C2C12 cells exhibited favorable attachment in the channels after 1 day of seeding....
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PMID: 20050808
PDF is available here.
PDF is available here.
Anticancer Research (30)10 2010
Abstract
We show that the concentration-dependent use of salen-manganese complexes within the appropriate time window and with parallel control of signaling parameters allows complex interactions during intercellular induction of apoptosis to be studied. At very low concentrations, salen-manganese complexes...
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PMID: 21036710
PDF is available here.
PDF is available here.
Experimental Dermatology (19)10 2010
Abstract
We investigated whether resveratrol would activate p66Shc in human immortalised keratinocytes (HaCaT cells), a well known and largely used model for skin keratinocytes. HaCaT cells were treated with resveratrol (10-150 μm) for different times. The effect of resveratrol on the proliferation of HaCaT...
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PMID: 20626463
PDF is available here.
PDF is available here.
Laboratory Investigation (90)10 2010
Abstract
We investigated the molecular mechanisms responsible for ErbB4-induced cell survival. In cultured mouse colon epithelial cells, ErbB4 overexpression resulted in increased levels of cyclooxygenase-2 (COX-2) mRNA and protein; in contrast, ErbB4 knockdown with siRNA blocked COX-2 accumulation in respon...
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PMID: 20585313
PDF is available here.
PDF is available here.
Abstract
Our study has demonstrated a wider proteome profile of E. coli and S. aureus mastitic milk whey, identified more low abundant defense proteins than reported before, and has linked for the first time identified proteins to several network functions and Biocarta pathways....
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PMID: 20704270
PDF is available here.
PDF is available here.
Abstract
We determined that LPS directly and reversibly affects cardiac myocyte function by altering regulation of intracellular Ca2+ concentration ([Ca2+]i) in immortalized cardiomyocytes, HL-1 cells. [Ca2+]i oscillated (<0.4 Hz), displaying slow and transient components. LPS (1 microg/ml), derived either f...
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PMID: 20573997
PDF is available here.
PDF is available here.
Abstract
We show that Sin1, an essential component of mTOR complex 2 (mTORC2), regulates B cell development. Sin1 deficiency results in increased IL-7 receptor (il7r) and RAG recombinase (rag1 and rag2) gene expression, leading to enhanced pro-B cell survival and augmented V(D)J recombinase activity. We furt...
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PMID: 20705244
PDF is available here.
PDF is available here.
Abstract
We examined the structural integrity of genomic DNA after the combined treatment of cells with MMS and a PARP inhibitor, i.e., exposure to a sub-lethal dose of MMS in the presence of the PARP inhibitor 4-amino-1,8-napthalimide (4-AN). We used pulsed field gel electrophoresis (PFGE) for measurement o...
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PMID: 20573551
PDF is available here.
PDF is available here.
Abstract
Noroviruses and hepatitis A virus (HAV) are common causes of foodborne disease. They are usually shed in feces and have been found in sewage water, biosolids, and animal manures. With the wide application of manure and biosolids on agricultural lands, there is an increasing interest in investigating...
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PMID: 20455755
PDF is available here.
PDF is available here.
Abstract
Ropivacaine induced tonic inhibition of peak Na(+) currents of both subtypes in a dose- and frequency-dependent manner. rNav1.5 appeared to be more sensitive to ropivacaine. In addition, for both Na(+) channel subtypes, the steady-state inactivation curves, but not the activation curves, were signif...
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PMID: 20651810
PDF is available here.
PDF is available here.
Molecular Cell (39)2 2010
Abstract
We demonstrate that mTORC1 activation is sufficient to stimulate specific metabolic pathways, including glycolysis, the oxidative arm of the pentose phosphate pathway, and de novo lipid biosynthesis. This is achieved through the activation of a transcriptional program affecting metabolic gene target...
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PMID: 20670887
PDF is available here.
PDF is available here.
Abstract
We investigated the signaling pathways involved in inflammatory production caused by peptidoglycan (PGN), a cell wall component of the gram-positive bacterium, in BV-2 microglia. PGN caused a concentration- and time-dependent increase in inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (C...
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PMID: 20451669
PDF is available here.
PDF is available here.
Abstract
We designed a high-throughput western blot-based screen to examine the generation of the smallest N-terminal polyglutamine-containing Htt fragment. We screened 514 siRNAs targeting the repertoire of human protease genes. This screen identified 11 proteases that, when inhibited, reduced Htt fragment...
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PMID: 20670829
PDF is available here.
PDF is available here.
Oncology Reports (24)2 2010
Abstract
We found that the cells arising from human embryonic muscle could spontaneously transform into malignancy in vitro and we obtained 6 immortalized cell lines. In this study, polymerase chain reaction (PCR) was used to assay several tumor suppressor genes of these cell lines, and homozygous deletions...
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PMID: 20596646
PDF is available here.
PDF is available here.
Abstract
We investigated and characterized the effect of externally applied electric fields (EF) on retinal pigment epithelial (RPE) cells by exposing primary cultures of human RPE cells (hRPE) and those from the ARPE19 immortalized cell line to various strengths of EF (EF-treated cells) or to no EF (control...
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PMID: 20457155
PDF is available here.
PDF is available here.
Toxicology (275)1-3 2010
Abstract
We have previously reported that protein kinase C (PKC) theta (theta) and protein tyrosine kinase are involved in 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced apoptosis of L-MAT, a human lymphoblastic T cell line. In the current report, we show that Vav1, a GDP/GTP exchange factor for Rho-like...
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PMID: 20561557
PDF is available here.
PDF is available here.
Abstract
We show that high-voltage activated Ca(2+) channels are regulated by membrane phosphatidylinositol 4,5-bisphosphate (PIP(2)) with different sensitivities. Plasma membrane PIP(2) depletion by rapamycin-induced translocation of an inositol lipid 5-phosphatase or by a voltage-sensitive 5-phosphatase (V...
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PMID: 20670831
PDF is available here.
PDF is available here.
Abstract
We designed a high-throughput western blot-based screen to examine the generation of the smallest N-terminal polyglutamine-containing Htt fragment. We screened 514 siRNAs targeting the repertoire of human protease genes. This screen identified 11 proteases that, when inhibited, reduced Htt fragment...
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PMID: 20670829
PDF is available here.
PDF is available here.
Abstract
Actinic keratosis (AK) occurs on sun-exposed skin and may progress to invasive squamous cell carcinoma (SCC). As for its topical treatment, diclofenac/hyaluronic acid (HA) has been recently approved. The NSAID diclofenac is an inhibitor of COX-2; however, its mode of action in cutaneous epithelial c...
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PMID: 20237495
PDF is available here.
PDF is available here.
Abstract
Concurrent andrographolide treatment (10 microM, 3 h) sensitized Ras-transformed cells to radiation in vitro (sensitizer enhancement ratio, 1.73). Andrographolide plus radiation (one dose of 300 mg/kg peritumor andrographolide and one dose of 6 Gy radiation) resulted in significant tumor growth dela...
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PMID: 20610044
PDF is available here.
PDF is available here.
Brain research (1344) 2010
Abstract
Both over expression of cyclic AMP response element binding protein (CREB) in the nucleus accumbens (NAc), and intra-accumbal injection of cocaine- and amphetamine-regulated transcript (CART) peptides, have been shown to decrease cocaine reward. Also, over expression of CREB in the rat NAc increased...
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PMID: 20451507
PDF is available here.
PDF is available here.
Brain research (1344) 2010
Abstract
We investigated the influence of the -911C>A polymorphism (rs3761740) in the hydroxy-methyl-glutaryl CoA reductase (HMGCR) gene promoter on basal and regulated transcription, plasma cholesterol levels and the association with AD. Under in vitro conditions the A allele was found to be significantly m...
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PMID: 20450896
PDF is available here.
PDF is available here.
Abstract
We utilized surface plasmon resonance to measure binding affinities between all combinations of alternatively spliced beta-NRX 1-3 and NL 1-3 ectodomains. Binding was observed for all beta-NRX/NL pairs. The presence of the NL1 B splice insertion lowers beta-NRX binding affinity by approximately 2-fo...
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PMID: 20624592
PDF is available here.
PDF is available here.
Journal of Cell Biology (190)1 2010
Abstract
We use chemical genetics to show that the protein kinase Mps1 regulates both aspects of the SAC. Human MPS1-null cells were generated via gene targeting and reconstituted with either the wild-type kinase (Mps1(wt)) or a mutant version (Mps1(as)) sensitized to bulky purine analogues. Mps1 inhibition...
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PMID: 20624902
PDF is available here.
PDF is available here.
Abstract
These results suggest that chronic lithium and valproate treatments act differently from lamotrigine in respect of modulation of receptor- and/or capacitance-mediated Ca2+ flux. These differential effects on Ca2+ responses may be relevant to the distinctive clinical profiles of these mood stabilizer...
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PMID: 19400980
PDF is available here.
PDF is available here.
Abstract
We examined CRT and AMPK distribution in the kidney and the regulation of CRT by AMPK in cells. By immunofluorescence staining, we detected CRT at the apical pole in a polarized mouse S3 proximal tubule cell line and in native rat kidney proximal tubules, a distribution overlapping with AMPK. Two-el...
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PMID: 20462973
PDF is available here.
PDF is available here.
Abstract
We also found that B cell-activating factor of the tumor necrosis factor family is expressed in EBV-infected B cells in acute MS lesions and ectopic B-cell follicles. These findings support a role for EBV infection in B-cell activation in the MS brain and suggest that B cell-activating factor of the...
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PMID: 20535037
PDF is available here.
PDF is available here.
Abstract
We have used a transformed human bone marrow endothelial cell (TrHBMEC) line to study the potential impact of a lack of NQO1 activity on adhesion molecule [endothelial leukocyte adhesion molecule 1 (E-selectin), vascular cell adhesion molecule (VCAM)-1, and intercellular adhesion molecule (ICAM)-1]...
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PMID: 20378716
PDF is available here.
PDF is available here.
Abstract
We implemented a two-pronged approach, combining characterization of loss-of-function phenotypes of the Drosophila Nna1 ortholog (NnaD) with proteomics analysis of pcd mice. Reduced NnaD function yielded larval lethality, with survivors displaying phenotypes that mirror disease in pcd. Quantitative...
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PMID: 20620870
PDF is available here.
PDF is available here.
Journal of Neuroimmunology (225)1-2 2010
Abstract
We demonstrate that the pro-inflammatory lipid lysophosphatidylcholine (LPC) is capable of inducing microglial ROS production, which is mediated by the activity of NADPH oxidase. Inhibition of TRPV1 non-selective cation channels abolished ROS production in LPC-stimulated microglia, whereas inhibitor...
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PMID: 20554029
PDF is available here.
PDF is available here.
Abstract
I and hyaluronic acid synthase 3 expression levels were decreased by Carbylan GSX. This investigation demonstrates that Carbylan GSX may serve as a natural biomaterial for tissue-engineering of human vocal folds.
Copyright 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved....
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PMID: 20109588
PDF is available here.
PDF is available here.
Abstract
We have shown previously that the nature and activation state of fibroblasts are critical in modulating the invasive ability of ESCC in an in vivo-like organotypic 3D cell culture, a form of human tissue engineering. Dramatic differences in invasion of transformed esophageal epithelial cells depende...
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PMID: 20534479
PDF is available here.
PDF is available here.
Abstract
Our results suggest that increased risk of infant leukemia development in patients with NQO1 *1*3 polymorphism is partially dependent on the inhibition of p53 pathway, though further studies are needed to fully understand the pathological role of C465T variant in the development of childhood leukemi...
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PMID: 21073132
PDF is available here.
PDF is available here.
Abstract
Id1 (inhibitor of differentiation 1) is a member of the bHLH protein family. Consistent with its role in promoting proliferation and inhibiting differentiation, Id1 expression is low or negligible in normal prostate epithelial cells but is high in prostate cancer. Ectopic expression of Id1 in normal...
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PMID: 20186495
PDF is available here.
PDF is available here.
Abstract
We investigated the possible mechanistic aspects of this tenet by characterizing the effects of TFN using premalignant PWR-1E and malignant DU-145 human prostate epithelial cells. TFN promoted a dose- and time-dependent cytostasis or apoptosis induction in these cells. The cytostatic effects of TFN,...
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PMID: 20563249
PDF is available here.
PDF is available here.
Abstract
These EP-induced phenotypic changes in myofibroblasts indicate the potential of EP as a therapeutic agent in corneal wound healing....
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PMID: 20053976
PDF is available here.
PDF is available here.
Abstract
We previously showed that autophagy-defective immortalized mouse mammary epithelial cells are susceptible to metabolic stress, DNA damage, and genomic instability. We now report that autophagy deficiency is associated with endoplasmic reticulum (ER) and oxidative stress, and with deregulation of p62...
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PMID: 20530580
PDF is available here.
PDF is available here.