Find your PDFs fast.
Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Vero Cells (0):
Recent article
Vero Cells
Abstract
Abstract. A dengue vaccine effective against all four serotypes is urgently needed. However, safety and immunogenicity could be affected by prior exposure to flaviviruses. This open, controlled, phase IIa study was conducted in 35 healthy adults who had received monovalent, live attenuated Vero cell-...
|
PMID: 21976579
PDF is available here.
PDF is available here.
| < More recent | Older article > |
Abstract
Mechanism of virus inhibiting action against measles virus of polyelectrolytes (PE) polystyrolsulfonate (PSS) of various polymerization degree and 60 kDa molecular weight polyallylamine (PAA) was studied.
|
PMID: 21913389
PDF is available here.
PDF is available here.
Abstract
We recently characterized the interaction between the intraviral domains of envelope glycoproteins (Gn and Gc) and ribonucleoprotein (RNP) of Puumala and Tula hantaviruses (genus Hantavirus, family Bunyaviridae). Herein we report a direct interaction between spike-forming glycoprotein and nucleic ac...
|
PMID: 21807393
PDF is available here.
PDF is available here.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] (27)5 2011
Abstract
The study aimed to construct the amplicon vector of HSV-1 strain HF and explore its universal package function between different serotypes of HSV. OriS and pac elements were obtained by enzyme digestion from the Plasmid BAC-HSV-1 strain HF and sequenced. With red fluorescence (DsRed)...
|
PMID: 21998950
PDF is available here.
PDF is available here.
Ukrainskij biokhimicheskij zhurnal/Ukrayins'kij biokhimichnij zhurnal/Ukrainian biochemical journal (82)6 2011
Abstract
We suppose that diverse sensitivity to DT of monkey and mouse cells can be explained not only by differences in their receptor affinity for DT but also by the processes that occur after internalization of the toxin into the cells....
|
PMID: 21805864
PDF is available here.
PDF is available here.
Abstract
We employed a rational attenuation mechanism that also prevents the infection of mosquito vectors. The internal ribosome entry site (IRES) from encephalomyocarditis virus replaced the subgenomic promoter in a cDNA CHIKV clone, thus altering the levels and host-specific mechanism of structural protei...
|
PMID: 21829348
PDF is available here.
PDF is available here.
Abstract
We isolated a single amino acid point mutant that is selectively defective in host shutoff activity. Incorporation of this mutation into MHV68 yielded a virus with significantly reduced capacity for mRNA turnover. Unexpectedly, the MHV68 mutant showed little defect during the acute replication phase...
|
PMID: 21811408
PDF is available here.
PDF is available here.
Abstract
The study was targeted to investigate the propagation of rubella virus in the cell cultures of various origins and with different cultivation methods. The high-yielding strain of rubella virus was produced. The "spinner-culture" cultivation method was applied and the strain's RNA was...
|
PMID: 21899118
PDF is available here.
PDF is available here.
Abstract
Abstract. Three tetravalent formulations of chimeric dengue (DENVax) viruses containing the pre-membrane and envelope genes of serotypes 1-4 expressed by the attenuated DENV-2 PDK-53 genome were tested for safety, immunogenicity, and efficacy in cynomolgus macaques (Macaca fascicular...
|
PMID: 21633037
PDF is available here.
PDF is available here.
Abstract
We characterized the F strain of herpes simplex virus-1 passaged in the presence of a natural carrageenan isolated from the red seaweed Gigartina skottbergii in view of the virulence for mice of isolated viral clones.
Viral clones (syn14-1 and syn17-2) showed a syncytial phenotype an...
|
PMID: 22256337
PDF is available here.
PDF is available here.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] (27)3 2011
Abstract
We constructed the plasmid C223-UL43-left-arms-UL47-right-arms which carried the homologous sequences of HSV-1. Liposome embedding method was used to transfect HSV-1 genome and the plasmid C223-UL43-left-arms-UL47-right-arms linearized by Mlu I digestion into Vero cells. After the successful homolog...
|
PMID: 21774249
PDF is available here.
PDF is available here.
Analytical Biochemistry (411)1 2011
Abstract
Guanidinium thiocyanate-phenol-chloroform extraction (GTPC extraction) is widely used in molecular biology for isolating DNA, RNA, and proteins. Protein isolation by commercially available GTPC solutions is time consuming and the resulting pellets are only incompletely soluble. In th...
|
PMID: 21094121
PDF is available here.
PDF is available here.
Abstract
We show that RON2 is inserted as an integral membrane protein in the host cell and, using several interaction assays with native or recombinant proteins, we define the region that binds AMA1. Our studies were performed both in Toxoplasma gondii and Plasmodium falciparum and although AMA1 and RON2 pr...
|
PMID: 21347343
PDF is available here.
PDF is available here.
Abstract
A dengue nonstructural protein 1 (NS1) antigen capture enzyme-linked immunosorbent assay (ELISA)-based tissue culture infectious dose-50 (TCID(50)) test (TCID(50)-ELISA) was developed as an alternative to the standard plaque assay for titrating dengue virus. Virus titers obtained by...
|
PMID: 21799894
PDF is available here.
PDF is available here.
Abstract
Vero cells were infected with R849 and HF simultaneously and two viruses, RH1 and RH2, expressing the LacZ gene and inducing extensive cell fusion were selected. A polymerase chain reaction (PCR)-based analysis suggested that one copy of the gamma 34.5 gene is lost in RH1, whereas both copies are lo...
|
PMID: 21663640
PDF is available here.
PDF is available here.
Abstract
We present a convenient approach for the synthesis of two irregular 3-O-sulfonated heparan sulfate octasaccharides, making use of a key disaccharide intermediate to acquire different building blocks for the oligosaccharide chain assembly. Despite substantial structural differences, the prepared 3-O-...
|
PMID: 21697878
PDF is available here.
PDF is available here.
Abstract
We demonstrate that 9b in the absence of any nuclear localization signal (NLS) enters the nucleus by passive transport. Using various cell cycle inhibitors, we have shown that the nuclear entry of 9b is independent of the cell cycle. Further, we found that 9b interacts with the cellular protein Crm1...
|
PMID: 21637748
PDF is available here.
PDF is available here.
Abstract
We describe how to handle, process, and screen field-collected mosquitoes for infectious virus by Vero cell culture assay. Mosquitoes are sorted by trap location and species, and grouped into pools containing ≤50 individuals. Pooled specimens are homogenized in buffered saline using a mixer-mill a...
|
PMID: 21694689
PDF is available here.
PDF is available here.
Acta Virologica (55)1 2011
Abstract
We kept mice on a Se-replete or Se-deficient diet for 28 days, infected them intraperitoneally with CVB3 and maintaining previous diets, we examined them for next 90 days for several parameters indicative of the infection or disease. We found out that the mice on the Se-deficient diet exhibited a ...
|
PMID: 21434702
PDF is available here.
PDF is available here.
Abstract
We demonstrate that original human influenza viruses infect primary human nasal epithelial cells at acidic pH (down to 5.4), whereas H5N1 HPAIVs lose infectivity at pH≤5.6. The HA of A/Vietnam/1203/04 was modified by introducing the single substitution HA2 58K→I, decreasing the pH of the HA conf...
|
PMID: 21490925
PDF is available here.
PDF is available here.
Acta Virologica (55)1 2011
Abstract
Small interfering RNA (siRNA) molecules are considered to be a promising antiviral therapeutics. This study was performed to analyze the application of siRNA against infectious bursal disease virus (IBDV) replication. Two siRNAs were designed to target common coding sequences of f...
|
PMID: 21434708
PDF is available here.
PDF is available here.
Acta Virologica (55)1 2011
Abstract
We addressed the question of transplacental transmission of MHV-68 from pregnant Balb/c mice chronically infected with the virus to their fetuses and shedding of the virus by breast milk from chonically infected mothers to their offspring. The mothers were positive to infectious virus and viral anti...
|
PMID: 21434705
PDF is available here.
PDF is available here.
Abstract
Methanol extract of Indigofera linnaei (MEIL) was investigated for antitumor, cytotoxic and antioxidant activities against transplantable tumors and human cancer cell lines. In vitro cytotoxicity was evaluated in HeLa, Hep-2, HepG-2, MCF-7, HT-29, Vero and NIH 3T3 cells by MTT assay and in vivo antit...
|
PMID: 21627353
PDF is available here.
PDF is available here.
Abstract
Study in CBA line mice of immunogenicity of cold adapted reassortant influenza virus H5N2 vaccine strain samples produced in rollers in MDCK and Vero cell cultures by using plant derived components.
Antibody levels in blood sera and nasal swabs, lungs and small intest...
|
PMID: 21812141
PDF is available here.
PDF is available here.
Abstract
To study the optimal conditions for roller cultivation of cold-adapted reassortant vaccine strain of influenza virus A/17/Duck/ Potsdam/86/92 (H5N2) in MDCK and Vero cell cultures grown on nutrient medium based on soy and rice flour hydrolysates obtained using trypsin and bromeline.
|
PMID: 21598624
PDF is available here.
PDF is available here.
Abstract
Efficacy of candidate DNA-vaccines based on the variola virus natural gene A30L and artificial gene A30Lopt with modified codon usage, optimized for expression in mammalian cells, was tested. The groups of mice were intracutaneously immunized three times with three-week intervals with candidate DNA-v...
|
PMID: 21786633
PDF is available here.
PDF is available here.
Biocontrol Science (15)4 2010
Abstract
We conclude that KE possesses potent anti PRV activity, and the candidate responsible for the antiviral property was the polysaccharide fraction....
|
PMID: 21212504
PDF is available here.
PDF is available here.
Abstract
An outbreak of peste des petits ruminants (PPR) was recorded in Kalubia province, Egypt in 2006, affecting a large population of migratory goats and sheep over a huge geographical area. Epidemiological, clinical and laboratory investigations were performed. Diseased animals showed pyrexia, erosive st...
|
PMID: 21309463
PDF is available here.
PDF is available here.
Abstract
2-[4-(4-Methoxyphenylcarbonyloxy)benzylidene]-6-dime-thylaminomethyl cyclohexanone hydrochloride 1 has a MIC value of 0.78 microg/mL towards Mycobacterium tuberculosis H37Rv and displays similar or identical MIC figures towards various drug-resistant strains of this microorganism. The enone 1 along...
|
PMID: 21155394
PDF is available here.
PDF is available here.
Abstract
We show that non-muscle myosin heavy chain IIA (NMHC-IIA), a subunit of non-muscle myosin IIA (NM-IIA), functions as an HSV-1 entry receptor by interacting with gB. A cell line that is relatively resistant to HSV-1 infection became highly susceptible to infection by this virus when NMHC-IIA was over...
|
PMID: 20944748
PDF is available here.
PDF is available here.
Abstract
We show that Granada virus infects humans. The analysis of the complete genome of Granada virus revealed that this agent is likely to be a natural reassortant of the recently described Massilia virus (donor of the long and short segments) with a yet unidentified phlebovirus (donor of the medium segm...
|
PMID: 20889862
PDF is available here.
PDF is available here.
Abstract
These results indicate that CD122+ cells (probably NKT cells) play a crucial role in host defense by activating CD4+ T cells against N. caninum infection....
|
PMID: 20460838
PDF is available here.
PDF is available here.
Abstract
Purine analogs modified in the five-membered ring have been synthesized and examined for antibacterial activity against Mycobacterium tuberculosis H(37)Rv in vitro employing the microplate alamar blue assay (MABA). The 9-deaza analogs were only found to be weak inhibitors, but the 8-aza-, 7-deaza- a...
|
PMID: 20833056
PDF is available here.
PDF is available here.
Abstract
We evaluated the role of gE-2 in the virus lifecycle by deleting amino acids 124-495 (gE2-del virus). In the mouse retina infection model, gE2-del virus does not spread to nuclei in the brain, indicating a defect in anterograde (pre-synaptic to post-synaptic neurons) and retrograde (post-synaptic to...
|
PMID: 20598729
PDF is available here.
PDF is available here.
Abstract
We analyzed four amino acid residues within domain VI of the Sendai virus L protein and our data indicated that there could be differences in L protein sequence requirements for cap methylation in two different families of Mononegavirales - rhabdoviruses and paramyxoviruses. In this study, we conduc...
|
PMID: 20609457
PDF is available here.
PDF is available here.
Abstract
As a continuation of our research and with the aim of obtaining new anti-tuberculosis agents which can improve the current chemotherapeutic anti-tuberculosis treatments, forty-three new quinoxaline-2-carboxamide 1,4-di-N-oxide derivatives were synthesized and evaluated for in vitro anti-tuberculosis...
|
PMID: 20656380
PDF is available here.
PDF is available here.
Abstract
Historical manufacturing data can potentially harbor a wealth of information for process optimization and enhancement of efficiency and robustness. To extract useful data multivariate data analysis (MVDA) using projection methods is often applied. In this contribution, the results obtained from appl...
|
PMID: 20506395
PDF is available here.
PDF is available here.
Abstract
We also demonstrated that glutamine was a major carbon source to supply the TCA cycle in Vero cells and that a reduction of lactate production did not necessary improve the efficiency of the Vero cell metabolism. Thus, to adapt the formulation of the medium to the Vero cell needs, it is important to...
|
PMID: 20506276
PDF is available here.
PDF is available here.
Abstract
Chalcogenide derivatives of three aminomethylphosphines: P(CH2N(CH2CH2)2NCH3)3 (1), P(CH2N(CH2CH2)2NCH2CH3)3 (2) and P(CH2N(CH2CH2)2O)3 (3) were prepared: oxides--OP(CH2N(CH2CH2)2NCH3)3 (4), OP(CH2N(CH2CH2)2NCH2CH3)3 (5), OP(CH2N(CH2CH2)2O)3 (6), sulfides--SP(CH2N(CH2CH2)2NCH3)3 (7), SP(CH2N(CH2CH2)...
|
PMID: 20617235
PDF is available here.
PDF is available here.
Abstract
We reported the synthesis and evaluation of anti-Toxoplasma gondii and antimicrobial activities in vitro of three new compound series obtained from ethyl(5-methyl-1-H-imidazole-4-carboxylate): acylthiosemicarbazide analogues 3a-d, 4-thiazolidinone analogues 4a-d and 1,3,4-thiadiazole analogues 5a-d....
|
PMID: 20541294
PDF is available here.
PDF is available here.
Abstract
A proteomic approach was used to investigate the dynamic cellular host cell response induced by influenza virus infection in two different vaccine production cell lines, MDCK and Vero. For identification of proteins possibly involved in global host cell response mechanisms and virus-host cell intera...
|
PMID: 20435171
PDF is available here.
PDF is available here.
Abstract
We show that resveratrol inhibited cholera toxin (CT)-induced cyclic AMP accumulation in Vero cells. Resveratrol suppressed the CT activity by suppressing the internalization of CT and traffic to the Golgi apparatus without affecting CT binding to cells. Further, resveratrol partially precipitated C...
|
PMID: 20307562
PDF is available here.
PDF is available here.
Abstract
We screened a variety of wild-type (WT) rhabdoviruses against a panel of tumor cell lines. Our screen identified a number of viruses with varying degrees of killing activity. Maraba virus was the most potent of these strains. We built a recombinant system for the Maraba virus platform, engineered a...
|
PMID: 20551913
PDF is available here.
PDF is available here.
Abstract
We investigated the cytotoxicity and interaction with intestinal tissues of an SF, Shiga-toxin-negative E. coli O157:NM isolate. This bovine isolate was negative for stx genes and was not cytotoxic for Vero cells. We found that this E. coli O157 isolate possesses an intimin of type beta, whereas the...
|
PMID: 20482230
PDF is available here.
PDF is available here.
Genes & Development (406)1 2010
Abstract
We tested two critical hypotheses about the mechanism and significance of lamina disruption. First, we show that chemical inhibition of all PKC isoforms reduced viral growth five-fold and inhibited capsid egress from the nucleus. However, specific inhibition of either conventional PKCs or PKC delta...
|
PMID: 20674954
PDF is available here.
PDF is available here.
Abstract
We show that VP22 physically interacted with infected cell polypeptide 0 (ICP0) and colocalized in the nucleus, indicating that VP22 could be functionally involved in the modulation of viral transcription through interaction with ICP0. In the HSV-1 infection system and chloramphenicol acetyltransfer...
|
PMID: 20457214
PDF is available here.
PDF is available here.
Abstract
We report an adenosine nucleoside prodrug that potently inhibits DENV replication both in cell culture and in a DENV mouse model. NITD449 (2'-C-acetylene-7-deaza-7-carbamoyladenosine) was initially identified as a parental compound that inhibits all four serotypes of DENV with low cytotoxicity. Howe...
|
PMID: 20516277
PDF is available here.
PDF is available here.
Virus Research (151)2 2010
Abstract
This short communication shows how the application of simple mathematical formulae allows researchers to extract maximum information from viral growth efficiency studies at virtually no additional costs (in terms of time or money), thus improving the comparability of results (growth rates, replicati...
|
PMID: 20580755
PDF is available here.
PDF is available here.
Genes & Development (406)1 2010
Abstract
We generated an HPIV2 virus in which P and V are expressed from separate genes (rHPIV2-P+V). rHPIV2-P+V replicated like HPIV2-WT in vitro and in non-human primates. HPIV2-P+V was modified by introducing two separate mutations into the V protein to create rHPIV2-L101E/L102E and rHPIV2-Delta122-127. I...
|
PMID: 20667570
PDF is available here.
PDF is available here.
Abstract
We developed a direct xenograft model, UM-PEL-1, by transferring freshly isolated human PEL cells into the peritoneal cavities of NOD/SCID mice without in vitro cell growth to avoid the changes in KSHV gene expression evident in cultured cells. We used this model to show that bortezomib induces PEL...
|
PMID: 20615981
PDF is available here.
PDF is available here.
Abstract
Site-directed mutagenesis of residues in the BC loop (residues 329-333) of the envelope (E) protein domain III in a West Nile virus (WNV) infectious clone and in plasmids encoding recombinant WNV and dengue type 2 virus domain III proteins demonstrated a critical role for residues in this loop in th...
|
PMID: 20447672
PDF is available here.
PDF is available here.
Abstract
These results demonstrate that the sequence of vaccination affects the expansion of cross-reactive CD8(+) T cells after heterologous chimeric flavivirus challenge....
|
PMID: 20536361
PDF is available here.
PDF is available here.
Abstract
We carried out lead optimization and structure-activity studies to provide a series of improved inhibitors that show potent PLpro inhibition and antiviral activity against SARS-CoV infected Vero E6 cells. Interestingly, the (S)-Me inhibitor 15 h (enzyme IC(50) = 0.56 microM; antiviral EC(50) = 9.1 m...
|
PMID: 20527968
PDF is available here.
PDF is available here.
Journal of Bacteriology (192)13 2010
Abstract
We report that the Rickettsia typhi genome possesses two genes encoding patatin-like PLA(2) proteins, RT0590 and RT0522. Sequence analysis of RT0522 and RT0590 reveals the presence of the conserved motifs essential for PLA(2) activity. Transcriptional analysis indicates that RT0522, but not RT0590,...
|
PMID: 20435729
PDF is available here.
PDF is available here.
Virus Research (151)1 2010
Abstract
We identified a unique amino acid difference (Val(1994)-Ile(1994)) that distinguished the more virulent strains, Anhui1 (Ah1), Henan1 (Hn1) and Henan2 (Hn2) from the less virulent strains, Chongqing1 (Cq1), Chongqing2 (Cq2) and Chongqing3 (Cq3). This amino acid difference is located in the finger do...
|
PMID: 20398708
PDF is available here.
PDF is available here.
Journal of Virology (84)13 2010
Abstract
We performed in vitro binding assays with N-terminal proteins encoding amino acids 1 to 160 of wild-type ICP27 or arginine-to-lysine substitution mutants. The R138,148,150K triple mutant bound weakly to sequences that were bound by the wild-type protein and single and double mutants. Furthermore, du...
|
PMID: 20410270
PDF is available here.
PDF is available here.
Journal of Virology (84)13 2010
Abstract
We investigated the molecular mechanisms by which the P gene products of the neurovirulent CDV A75/17 strain disrupt type I interferon (IFN-alpha/beta)-induced signaling that results in the establishment of the antiviral state. Using recombinant knockout A75/17 viruses, the V protein was identified...
|
PMID: 20427537
PDF is available here.
PDF is available here.
Journal of General Virology (91)Pt 7 2010
Abstract
Chimeric yellow fever virus 17D (YFV-17D) and dengue virus type 2 (DENV2) carrying the surface proteins of Modoc virus (MODV), a not-known-vector (NKV) flavivirus, replicated efficiently in mammalian (Vero-B) and mosquito (C6/36) cells, whereas MODV failed to replicate in mosquito ce...
|
PMID: 20219898
PDF is available here.
PDF is available here.
Abstract
The plants of the Asteraceae family have been used for medicinal purposes,in traditional Colombian medicine.
To evaluate the antifungal activity and the cytotoxic effects of 15 essential oils from plants of the Asteraceae family.
Antifungal activity was evaluated against Candida parapsilosis ATCC 22...
|
PMID: 20346291
PDF is available here.
PDF is available here.
Abstract
HSV-1 virions contain a proteinaceous layer termed the tegument that lies between the nucleocapsid and viral envelope. The molecular mechanisms that facilitate incorporation of tegument proteins are poorly characterized. The tegument protein VP22 interacts with VP16 and the cytoplasm...
|
PMID: 20580397
PDF is available here.
PDF is available here.