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Cells, Immobilized (0):
Recent article
Cells, Immobilized
Abstract
Increasing interest in biological surfactants has led to intensified research directed at more cost-efficient production of biosurfactants, relative to traditional surface-active components based on petrochemical feedstocks. This publication will focus on a new integrated process for...
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PMID: 21567991
PDF is available here.
PDF is available here.
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Water Research (44)18 2010
Abstract
The main objective of this research is to model the effect of biodegradation process of phenol at high initial concentrations using a well known immobilization technique of the biomass. This work focused on testing the effect of activated carbon and clay while considering the diffusive internal mass...
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PMID: 20615523
PDF is available here.
PDF is available here.
Abstract
One of the first strategies for cancer gene therapy was the use of suicide gene/prodrug combinations, originally delivered to tumor cells using viral vectors. A major limitation of this approach was the inefficiency of suicide gene delivery. An alternative strategy, in which the suicide genes are ph...
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PMID: 20677096
PDF is available here.
PDF is available here.
Abstract
Isomaltulose was obtained from sucrose solution by immobilized cells of Erwinia sp. D12 using a batch and a continuous process. Parameters for sucrose conversion into isomaltulose were evaluated using both experimental design and response surface methodology. Erwinia sp. D12 cells were immobilized i...
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PMID: 20135241
PDF is available here.
PDF is available here.
Abstract
Rhizopus oryzae immobilized in calcium alginate was applied in lactic acid fermentation with unhydrolyzed raw sweet potato powders as the sole carbon source. The effects of sodium alginate concentration, calcium chloride concentration, and the immobilized bead diameter on lactic acid production were...
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PMID: 20020223
PDF is available here.
PDF is available here.
Abstract
Low-cost dried yeasts immobilized on delignified brewers' spent grains for use in wine making and brewing were produced by simple thermal drying techniques. To optimize the thermal drying process, vacuum and air stream conditions were examined. Drying of thin layers of the biocatalysts was performed...
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PMID: 20091140
PDF is available here.
PDF is available here.
Biotechnology Letters (32)8 2010
Abstract
The production of biodiesel by transesterification employing acid or base catalyst has been industrially accepted for its high conversion and reaction rates. Downstream processing costs and environmental problems associated with biodiesel production and byproducts recovery have led to the search for...
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PMID: 20401680
PDF is available here.
PDF is available here.
Abstract
The cometabolic degradation of trichloroethene (TCE) by Rhodococcus sp. L4 was limited by the loss of enzyme activity during TCE transformation. This problem was overcome by repeated addition of inducing substrates, such as cumene, limonene, or cumin aldehyde, to the cells. Alternatively, Rhodococcu...
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PMID: 20472723
PDF is available here.
PDF is available here.
Abstract
We propose an alternative method of making hydrogel-encapsulated cell constructs through layer by layer (LBL) buildup of alternating layers of cells and hydrogel. The LBL method potentially permits better spatial control of different cell types and control of cell orientation. Each hydrogel layer mu...
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PMID: 20568698
PDF is available here.
PDF is available here.
Biomaterials (31)18 2010
Abstract
Hydrogels are increasingly used as a cell encapsulation and transplantation device. The successful use of a hydrogel greatly relies on an ability to control hydrogel stiffness which affects structural integrity and regulates cellular phenotypes. However, conventional strategies to increase the gel s...
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PMID: 20347136
PDF is available here.
PDF is available here.
Abstract
Immobilized cells of Delftia tsuruhatensis CCTCC M 205114 harboring R-amidase were applied in asymmetric hydrolysis of (R)-2, 2-dimethylcyclopropane carboxamide (R - 1) from racemic (R, S)-2, 2-dimethylcyclopropane carboxamide to accumulate (S)-2, 2-dimethylcyclopropane carboxamide (S - 1). Maximum...
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PMID: 20177734
PDF is available here.
PDF is available here.
Biotechnology Letters (32)5 2010
Abstract
Recombinant Escherichia coli cells, over-expressing cyclopentanone monooxygenase activity, were immobilized in polyelectrolyte complex capsules, made of sodium alginate, cellulose sulfate, poly(methylene-co-guanidine), CaCl(2) and NaCl. More than 90% of the cell viability was preserved during the en...
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PMID: 20111981
PDF is available here.
PDF is available here.
Bioresource Technology (101)7 2010
Abstract
The immobilization of Pseudomonas stutzeri using adsorption on different inorganic supports was studied in relation to the number of adsorbed cells, metabolic activity and biodesulfurization (BDS). The electrophoretic migration (EM) measurements and Tetrazolioum (TTC) method were used to evaluate ad...
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PMID: 20034786
PDF is available here.
PDF is available here.
Abstract
We hoped to increase the selectivity of the assay. Based on the observed patterns of response, we demonstrate that the sensor can be used for the qualitative and, in some concentrations, quantitative detection of the pesticides with a high degree of reproducibility. The lowest detected concentration...
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PMID: 20152413
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
A novel configuration of photobioreactor is described in which filaments of alginate containing immobilized cells of a leaky mutant of Dunaliella parva are wound round a central light well which is located within a glass outer chamber so that a liquid medium is caused to flow in the...
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PMID: 19838629
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
Immobilization of cis-epoxysuccinate hydrolase-containing E. coli for D(-)-tartaric acid production was screened by various methods. The highest recovery of activity was obtained by entrapment in kappa-carrageenan gel. 23.6 g biomass/l and 43.4 g kappa-carrageenan/l were the best imm...
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PMID: 19844664
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
A novel configuration of photobioreactor is described in which filaments of alginate containing immobilized cells of a leaky mutant of Dunaliella parva are wound round a central light well which is located within a glass outer chamber so that a liquid medium is caused to flow in the...
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PMID: 19838629
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
Immobilization of cis-epoxysuccinate hydrolase-containing E. coli for D(-)-tartaric acid production was screened by various methods. The highest recovery of activity was obtained by entrapment in kappa-carrageenan gel. 23.6 g biomass/l and 43.4 g kappa-carrageenan/l were the best imm...
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PMID: 19844664
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
A novel configuration of photobioreactor is described in which filaments of alginate containing immobilized cells of a leaky mutant of Dunaliella parva are wound round a central light well which is located within a glass outer chamber so that a liquid medium is caused to flow in the...
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PMID: 19838629
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
Immobilization of cis-epoxysuccinate hydrolase-containing E. coli for D(-)-tartaric acid production was screened by various methods. The highest recovery of activity was obtained by entrapment in kappa-carrageenan gel. 23.6 g biomass/l and 43.4 g kappa-carrageenan/l were the best imm...
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PMID: 19844664
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
A novel configuration of photobioreactor is described in which filaments of alginate containing immobilized cells of a leaky mutant of Dunaliella parva are wound round a central light well which is located within a glass outer chamber so that a liquid medium is caused to flow in the...
|
PMID: 19838629
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
Immobilization of cis-epoxysuccinate hydrolase-containing E. coli for D(-)-tartaric acid production was screened by various methods. The highest recovery of activity was obtained by entrapment in kappa-carrageenan gel. 23.6 g biomass/l and 43.4 g kappa-carrageenan/l were the best imm...
|
PMID: 19844664
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
A novel configuration of photobioreactor is described in which filaments of alginate containing immobilized cells of a leaky mutant of Dunaliella parva are wound round a central light well which is located within a glass outer chamber so that a liquid medium is caused to flow in the...
|
PMID: 19838629
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
Immobilization of cis-epoxysuccinate hydrolase-containing E. coli for D(-)-tartaric acid production was screened by various methods. The highest recovery of activity was obtained by entrapment in kappa-carrageenan gel. 23.6 g biomass/l and 43.4 g kappa-carrageenan/l were the best imm...
|
PMID: 19844664
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
A novel configuration of photobioreactor is described in which filaments of alginate containing immobilized cells of a leaky mutant of Dunaliella parva are wound round a central light well which is located within a glass outer chamber so that a liquid medium is caused to flow in the...
|
PMID: 19838629
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
A novel configuration of photobioreactor is described in which filaments of alginate containing immobilized cells of a leaky mutant of Dunaliella parva are wound round a central light well which is located within a glass outer chamber so that a liquid medium is caused to flow in the...
|
PMID: 19838629
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
A novel configuration of photobioreactor is described in which filaments of alginate containing immobilized cells of a leaky mutant of Dunaliella parva are wound round a central light well which is located within a glass outer chamber so that a liquid medium is caused to flow in the...
|
PMID: 19838629
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
A novel configuration of photobioreactor is described in which filaments of alginate containing immobilized cells of a leaky mutant of Dunaliella parva are wound round a central light well which is located within a glass outer chamber so that a liquid medium is caused to flow in the...
|
PMID: 19838629
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
Immobilization of cis-epoxysuccinate hydrolase-containing E. coli for D(-)-tartaric acid production was screened by various methods. The highest recovery of activity was obtained by entrapment in kappa-carrageenan gel. 23.6 g biomass/l and 43.4 g kappa-carrageenan/l were the best imm...
|
PMID: 19844664
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
Immobilization of cis-epoxysuccinate hydrolase-containing E. coli for D(-)-tartaric acid production was screened by various methods. The highest recovery of activity was obtained by entrapment in kappa-carrageenan gel. 23.6 g biomass/l and 43.4 g kappa-carrageenan/l were the best imm...
|
PMID: 19844664
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
Immobilization of cis-epoxysuccinate hydrolase-containing E. coli for D(-)-tartaric acid production was screened by various methods. The highest recovery of activity was obtained by entrapment in kappa-carrageenan gel. 23.6 g biomass/l and 43.4 g kappa-carrageenan/l were the best imm...
|
PMID: 19844664
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
Immobilization of cis-epoxysuccinate hydrolase-containing E. coli for D(-)-tartaric acid production was screened by various methods. The highest recovery of activity was obtained by entrapment in kappa-carrageenan gel. 23.6 g biomass/l and 43.4 g kappa-carrageenan/l were the best imm...
|
PMID: 19844664
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
A novel configuration of photobioreactor is described in which filaments of alginate containing immobilized cells of a leaky mutant of Dunaliella parva are wound round a central light well which is located within a glass outer chamber so that a liquid medium is caused to flow in the...
|
PMID: 19838629
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
Immobilization of cis-epoxysuccinate hydrolase-containing E. coli for D(-)-tartaric acid production was screened by various methods. The highest recovery of activity was obtained by entrapment in kappa-carrageenan gel. 23.6 g biomass/l and 43.4 g kappa-carrageenan/l were the best imm...
|
PMID: 19844664
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2010
Abstract
A novel configuration of photobioreactor is described in which filaments of alginate containing immobilized cells of a leaky mutant of Dunaliella parva are wound round a central light well which is located within a glass outer chamber so that a liquid medium is caused to flow in the...
|
PMID: 19838629
PDF is available here.
PDF is available here.
Abstract
Zymomonas mobilis was immobilized in a modified graphite felt cathode with neutral red (NR-graphite cathode) and Saccharomyces cerevisiae was cultivated on a platinum plate anode to electrochemically activate ethanol fermentation. Electrochemical redox reaction was induced by 3 approximately 4 volt...
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PMID: 20134239
PDF is available here.
PDF is available here.
Abstract
Lactic acid (LA) fermentation by Lactobacillus salivarius ATCC 11741 immobilized on loofa sponge (LS) was evaluated. To increase the surface area of LS for cell immobilization, H2O2 and chitosan were introduced as surface modifying reagents. Four chitosan of different molecular weight were separatel...
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PMID: 20134241
PDF is available here.
PDF is available here.
Abstract
The performance of a fluidized bed reactor using immobilized Phanerochaete chrysosporium to remove 2,4-dichlorophenol (2,4-DCP) from aqueous solution was investigated. The contribution of lignin peroxidase (LiP) and manganese peroxidase (MnP) secreted by Phanerochaete chrysosporium to the 2,4-DCP de...
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PMID: 20729600
PDF is available here.
PDF is available here.
Abstract
We encapsulated the cells within alginate microcapsules coated with each oligochitosan and a complete morphological and mechanical evaluation was performed. Finally, the in vivo functionality of the enclosed cells in the alginate-oligochitosan microcapsules was studied in Balb/c mice. Results show t...
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PMID: 19145624
PDF is available here.
PDF is available here.
Abstract
This study integrated dielectrophoresis (DEP) with non-flow through biochips to enhance the immuno-capture and detection of foodborne pathogenic bacteria. It demonstrated two major functions provided by DEP to improve the chip performance: (i) concentrating bacterial cells from the suspension to dif...
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PMID: 19836519
PDF is available here.
PDF is available here.
Environmental Science (30)10 2009
Abstract
A new kind of ammonia-oxidizing bacteria (AOB)-Comamonas aquatic LNL3 was screened out and immobilized by Poly (HEA)-Poly (HEMA) copolymer carrier using irradiation techniques. Four kinds of impact factors on short-cut nitrification, including temperature, pH, DO and free ammonia (FA) concentration...
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PMID: 19968113
PDF is available here.
PDF is available here.
Abstract
Our studies revealed that arthroconidia produced in the extracellular material might play an important role in keeping the long-term viability of the immobilized microorganism. After the arthroconidia were activated, an electrochemical biochemical oxygen demand (BOD) sensor based on cell/hybrid mate...
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PMID: 19726176
PDF is available here.
PDF is available here.
Biotechnology Letters (32)2 2009
Abstract
Immobilization of cis-epoxysuccinate hydrolase-containing E. coli for D(-)-tartaric acid production was screened by various methods. The highest recovery of activity was obtained by entrapment in kappa-carrageenan gel. 23.6 g biomass/l and 43.4 g kappa-carrageenan/l were the best imm...
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PMID: 19844664
PDF is available here.
PDF is available here.
Abstract
This study was conducted to investigate the applicability of the stimulant conditions for the bioluminescence activity of a recombinant strain of Pseudomonas putida, mt-2 KG1206, when immobilized using alginate polymer. The bioluminescence activity of the immobilized strain was generally approximate...
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PMID: 19603161
PDF is available here.
PDF is available here.
Abstract
Our findings suggest that it is feasible to immobilize RGD-containing peptides onto decellularized valve scaffolds. And the technique can effectively promote cell adhesion, which is beneficial for in vitro tissue engineering of heart valves....
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PMID: 19662371
PDF is available here.
PDF is available here.
Abstract
The percent removal and uptake of Pb2+ by algal-consortium (CP1) developed from wastewater of biological oxidation pond (Wazirabad, New Delhi, India) was studied under batch conditions with live biomass compared with continuous system using dried biomass immobilized on silica under laboratory condit...
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PMID: 19775077
PDF is available here.
PDF is available here.
Abstract
We have established MSC from adipose tissue and bone marrow in 3D cultures in alginate beads covalently modified with the tripeptide arginine-glycine-aspartic acid (RGD), the integrin-binding motif found in several molecules within the extracellular matrix. The MSC changed from their fibroblastoid s...
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PMID: 19115828
PDF is available here.
PDF is available here.
Abstract
The optimal condition for bacteriocin production and the number of cell cycle was with sodium alginate 2.8%, CaCl2 5%, immobilization time 4 h, the density of entrapped cells 1/20, culture time 63 h and inoculation volume 8.2%. The period of fermentation was 24 h of a batch fermentation after 63 h a...
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PMID: 19637577
PDF is available here.
PDF is available here.
Abstract
OBJECTIVE: To investigate the mechanism involved in aging process of immortalized human keratinocyte (HaCaT) and primary human epidermis keratinocyte of adults (HEKa) irradiated by ultraviolet B(UVB). METHODS: HEKa and HaCaT were repeatedly exposed to UVB at a subcytotoxic level. SA-beta-Gal stainin...
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PMID: 19504638
PDF is available here.
PDF is available here.
Abstract
We encountered the difficulty of significant removal of fungal mycelia along with the cellulase. To solve this problem, cell immobilization using cut pieces of hydrophilic polyurethane (PU) foam was evaluated. Five commercial PU foams with different pore sizes and porosities were tested. Two were fo...
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PMID: 19127442
PDF is available here.
PDF is available here.
Abstract
Whole-cell immobilization of selenate-respiring Sulfurospirillum barnesii in polyacrylamide gels was investigated to allow the treatment of selenate contaminated (790 microg Se x L(-1)) synthetic wastewater with a high molar excess of nitrate (1,500 times) and sulfate (200 times). Gel-immobilized S....
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PMID: 19305993
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (293)1 2009
Abstract
Cell surface display was used as a strategy to display the gold-binding polypeptide (GBP) fusion protein on the surface of Escherichia coli, and consequently to immobilize the cells on the gold surface. The DNA encoding the GBP was fused to the truncated fadL gene and was expressed by the tac promot...
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PMID: 19228193
PDF is available here.
PDF is available here.
Biomaterials (30)12 2009
Abstract
We propose an alternative method for microencapsulation of islets with a layer of living cells. We immobilized HEK293 cells (human endoderm kidney cell line) to the islet surface using amphiphilic poly(ethylene glycol)-conjugated phospholid derivative and biotin/streptavidin reaction and encapsulate...
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PMID: 19201021
PDF is available here.
PDF is available here.
Abstract
A simple method for the preparation of the biocatalyst with whole cells is presented, and the applicability of the technique for biodegradation of phenol in wastewater from the chemical industries using the basidomycetes yeast Trichosporon cutaneum is explored. Kinetic studies of the influence of ot...
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PMID: 19052785
PDF is available here.
PDF is available here.
Abstract
The biotransformation of acrylonitrile was investigated using thermophilic nitrilase produced from a new isolate Streptomyces sp. MTCC 7546 in both the free and immobilized state. Under optimal conditions,the enzyme converts nitriles to acids without the formation of amides.The whole cells of the is...
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PMID: 19430115
PDF is available here.
PDF is available here.
Abstract
We evaluated the stress of immobilization over bacteria cultures obtained from different growing states, the effect of cell density and bacteria capability to proliferate inside matrices. Best results to attain longer preservation times were obtained when we immobilized suspensions with an optimized...
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PMID: 19034444
PDF is available here.
PDF is available here.
Nitric Oxide (20)1 2009
Abstract
The aim of this work was to study the effects of resveratrol (RES) as compared to silymarin (SM) pretreatments on tert-butylhydroperoxide (tBH) induced apoptotic/necrotic markers in hepatocytes. Hepatocyte in cultures (48 h) and in perifused immobilized agarose threads (5h) were used as cellular sys...
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PMID: 18812229
PDF is available here.
PDF is available here.
Abstract
Arsenic(III) oxidation was evaluated in a continuous-flow fluidized-bed reactor (FBR) with Alcaligenes faecalis strain 01201 immobilized in gel beads. The FBR was operated under 300 mg/L citrate and a range of influent As(III) concentrations (75 to 3000 mg/L) at short hydraulic retention times (1.06...
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PMID: 19323288
PDF is available here.
PDF is available here.
Abstract
Cyanuric acid (1,3,5-triazine-2,4,6-triol [OOOT]) is a common biodegradation byproduct of triazinic herbicides, frequently accumulated in soils or water when supplementary carbon sources are absent. A binary bacterial culture able to degrade OOOT was selected through a continuous selection process a...
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PMID: 19002512
PDF is available here.
PDF is available here.
Abstract
The purpose of this investigation was to study the effect of Streptomyces erumpens cells immobilized in various matrices, i.e., agar-agar, polyacrylamide, and luffa (Luffa cylindrica L.) sponge for production of alpha-amylase. Luffa sponge was found to be 21% and 51% more effective in enzyme yield t...
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PMID: 18521760
PDF is available here.
PDF is available here.