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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Cell Membrane (6):
Recent article
Cell Membrane
Journal of Neurochemistry (119)5 2011
Abstract
We have used neuroblastoma cells expressing NDM29 at its basal level (Mock cells) or at 5.4-fold higher levels (S1 cells) to investigate whether a functional differentiation correlates with morphological and biochemical development induced by NDM29 expression. First, analyzing the expression of spec...
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PMID: 21933186
PDF is available here.
PDF is available here.
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Developmental Cell (21)4 2011
Abstract
We identified an alternative mode of cytokinin action that uses endocytic trafficking as a means to direct plant organogenesis. This activity occurs downstream of known cytokinin receptors but through a branch of the cytokinin signaling pathway that does not involve transcriptional regulation. We sh...
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PMID: 21962902
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (286)40 2011
Abstract
We analyzed early activation of murine CD4+ and CD8+ T-lymphocytes at 24 h. We observed a striking and selective up-regulation in the binding of a recombinant soluble form of siglec-E, an inhibitory siglec, which is expressed on several myeloid cell types including antigen-presenting dendritic cells...
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PMID: 21835922
PDF is available here.
PDF is available here.
Abstract
We investigated the effect of MSCs on immunophenotype of macrophages in the presence of an HA-hydrogel scaffold using a unique 3D coculture system. MSCs were encapsulated in the hydrogel and peripheral blood CD14+ monocyte-derived macrophages plated in direct contact with the MSC-gel construct. To d...
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PMID: 21554192
PDF is available here.
PDF is available here.
Abstract
We screened a set of proteins from the G-protein complex using two-hybrid complementation in yeast. After deep and exhaustive interrogation, we detected 544 interactions between 434 proteins, of which 68 highly interconnected proteins form the core G-protein interactome. Within this core, over half...
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PMID: 21952135
PDF is available here.
PDF is available here.
Developmental Cell (21)3 2011
Abstract
We report a role of Lgl1, the mammalian homolog of Drosophila tumor suppressor Lethal giant larvae, in controlling membrane trafficking underlying axonal growth. We find that Lgl1 is associated with plasmalemmal precursor vesicles and enriched in developing axons. Lgl1 upregulation promoted axonal g...
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PMID: 21856246
PDF is available here.
PDF is available here.
Proteomics (11)18 2011
Abstract
We present a simple, time- and cost-efficient approach to tackle the proteome of prokaryotic organisms. To obtain large data sets of complex biological experiments high-throughput and time- and cost-efficient methods still have to be developed and refined. In this study, we combined well-approved te...
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PMID: 21751368
PDF is available here.
PDF is available here.
Journal of Proteomics (74)8 2011
Abstract
Membrane proteins are an interesting class of proteins because of their functional importance. Unfortunately their analysis is hampered by low abundance and poor solubility in aqueous media. Since shotgun methods are high-throughput and partly overcome these problems, they are prefer...
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PMID: 21334471
PDF is available here.
PDF is available here.
Journal of Proteomics (74)8 2011
Abstract
Iron is essential for all living organisms and plays a crucial role in pathogenicity. This study presents the first proteome analysis of plasma membranes isolated from pea roots. Protein profiles of four different samples (+Fe, +Fe/Chitosan, -Fe, and -Fe/Chitosan) were compared by na...
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PMID: 21310270
PDF is available here.
PDF is available here.
Abstract
We show that a previously uncharacterized member of the I-BAR subfamily, Pinkbar, is specifically expressed in intestinal epithelial cells, where it localizes to Rab13-positive vesicles and to the plasma membrane at intercellular junctions. Notably, the BAR domain of Pinkbar does not induce membrane...
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PMID: 21743456
PDF is available here.
PDF is available here.
Abstract
We found for the first time that betaAP (25-35 fragment, 10-6 M) disturbs the processes of the resting membrane potential generation in muscle fibres, leading to depolarization by two mechanisms: 1) inhibition of Na+,K(+)-ATPase, which leads to loss of impact of this pump to the resting membrane pot...
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PMID: 21961303
PDF is available here.
PDF is available here.
Abstract
Abstract
We describe here the exosome-mediated release of small membrane vesicles containing the stem and cancer stem cell marker prominin-1 (CD133) during haematopoietic cell differentiation. Surprisingly, this contrasts with the budding mechanism underlying the release of this cholesterol-binding protein f...
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PMID: 21591261
PDF is available here.
PDF is available here.
Abstract
Ion channels in cell membranes are targets for a multitude of ligands including naturally occurring toxins, illicit drugs, and medications used to manage pain and treat cardiovascular, neurological, autoimmune, and other health disorders. In the past decade, the x-ray crystallography...
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PMID: 21961291
PDF is available here.
PDF is available here.
Biophysics (40)1 2011
Abstract
Over the past 14 years, ubiquitination has emerged as a centrally important mechanism governing the subcellular trafficking of proteins. Ubiquitination, interaction with sorting factors that contain ubiquitin-binding domains, and deubiquitination govern the itineraries of cargo prote...
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PMID: 21332354
PDF is available here.
PDF is available here.
Abstract
We discuss the various modes of intramembrane protein-lipid interactions in cellular membranes, including examples for both annular and nonannular bound lipids. Furthermore, we will discuss possible functional roles of such specific protein-lipid interactions as well as roles of lipids as chaperones...
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PMID: 21536707
PDF is available here.
PDF is available here.
Abstract
We provide a comprehensive review of the actions of BAs on cardiovascular function. In the heart and the systemic circulation, BAs interact with plasma membrane G-protein-coupled receptors, for example, TGR5 and muscarinic receptors, and nuclear receptors, for example, the farnesoid (FXR) and pregna...
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PMID: 21707953
PDF is available here.
PDF is available here.
Abstract
The Golgi apparatus in mammalian cells is positioned near the centrosome-based microtubule-organizing center (Fig. 1). Secretory cargo moves inward in membrane carriers for delivery to Golgi membranes in which it is processed and packaged for transport outward to the plasma membrane...
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PMID: 21504874
PDF is available here.
PDF is available here.
Abstract
We do not have a clear picture of their precise intracellular localization. Advances in lipidomics and subcellular fractionation should help to improve this situation. Genetic approaches have provided insights into the diversity of sterol and sphingolipid functions in eukaryotes providing evidence t...
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PMID: 21454248
PDF is available here.
PDF is available here.
Abstract
The ability to control stem cell differentiation is the holy grail of regenerative medicine. Although significant progress toward this goal has been achieved, few efficient and straightforward methods have been developed, necessitating a better understanding of the mechanisms that in...
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PMID: 21091341
PDF is available here.
PDF is available here.
Discovery medicine (11)59 2011
Abstract
Integrin αvβ3 is a heterodimeric structural protein of the plasma membrane that bears a cell surface receptor for thyroid hormone. The functions of this receptor are distinct from those of the classical nuclear receptor (TR) for thyroid hormone. The integrin is expressed primarily...
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PMID: 21524387
PDF is available here.
PDF is available here.
Abstract
Autism is a developmental disorder of the central nervous system characterized by impairments in social interaction, communication and restricted repetitive and stereotyped behavior. It is generally assumed that in most cases autism has a polygenic cause, but the pathogenesis is stil...
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PMID: 21375492
PDF is available here.
PDF is available here.
Proteomics (11)7 2011
Abstract
We report the subproteome reference maps of E. coli B REL606 by analyzing cytoplasmic, periplasmic, inner and outer membrane, and extracellular proteomes based on the genome information using experimental and computational approaches. Among the total of 3487 spots, 651 proteins including 410 non-red...
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PMID: 21337514
PDF is available here.
PDF is available here.
Abstract
We aimed to investigate how far nanoparticulates impair the function of the P-gp transport system and which particle properties govern these interactions. Expression and function of P-gp was confirmed in A549 cell monolayers. Rhodamine 123 (Rh123) release studies were carried out in the presence of...
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PMID: 21093586
PDF is available here.
PDF is available here.
Abstract
We found that hypoxia promoted Rab1 expression and strongly correlated with the repressed expression of PASMC phenotype markers in RPASMCs. These data strongly indicate that Rab1 modulates PASMCs function by manipulating AT1R traffic from the ER to the Golgi and provide the first evidence implicatin...
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PMID: 21095238
PDF is available here.
PDF is available here.
Journal of Proteomics (74)3 2011
Abstract
Milk fat globule membranes (MFGM) are three-layered structures that enclose fat droplets, and are composed by an internal monolayer of endoplasmic reticulum origin, surrounded by a bilayer derived from the apical membrane of the lactating cell. In this work, an optimized protein extr...
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PMID: 21147282
PDF is available here.
PDF is available here.
Journal of Proteomics (74)3 2011
Abstract
We performed here MS-based phosphoproteomics using both metal oxide affinity chromatography (pSTY proteomics) and anti-phosphotyrosine antibody (pY proteomics). The former method identified mainly phospho-serine and -threonine of nuclear or cytoplasmic proteins, whereas the latter did phosphotyrosin...
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PMID: 21118733
PDF is available here.
PDF is available here.
Abstract
We have developed a multivariate model capable of extracting combinations of markers that are the most informative to predict cellular age. To acquire signaling information with high temporal resolution, we designed a microfluidic chip enabling parallel lysis and fixation of stimulated cell samples...
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PMID: 21193537
PDF is available here.
PDF is available here.
Abstract
Rapid prototyping techniques are widely used to fabricate well-defined three-dimensional structures of tissue homologs. The piston-assisted microsyringe (PAM2) is a rapid prototyping technology specifically developed for low-shear stress extrusion of viscous hydrogel solutions contai...
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PMID: 20799910
PDF is available here.
PDF is available here.
Journal of Proteomics (74)2 2011
Abstract
Lipid rafts are microdomains of the phospholipid bilayer, proposed to form semi-stable "islands" that act as a platform for several important cellular processes; major classes of raft-resident proteins include signalling proteins and glycosylphosphatidylinositol (GPI)-anchored protei...
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PMID: 21070894
PDF is available here.
PDF is available here.
Abstract
Dynamic plasma membrane rearrangements occur during many cellular processes including endocytosis, morphogenesis, and migration. Actin polymerization together with proteins that directly deform membranes, such as the BAR superfamily proteins, is essential for generation of membrane i...
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PMID: 21093245
PDF is available here.
PDF is available here.
Developmental Cell (20)1 2011
Abstract
We show that the kinesin-3 motor KIF16B/Rab14 complex acts in biosynthetic Golgi-to-endosome traffic of the fibroblast growth factor receptor (FGFR) during early embryonic development. Kif16b(-/-) mouse embryos failed in developing epiblast and primitive endoderm lineages and died in the peri-implan...
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PMID: 21238925
PDF is available here.
PDF is available here.
Abstract
The acquisition of multidrug resistance poses a serious problem in chemotherapy, and new types of transporters have been actively sought to overcome it. In the present study, poly(ethylene glycol)-conjugated (PEGylated) multi-walled carbon nanotubes (MWCNTs) were prepared and explored as drug carrie...
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PMID: 20970441
PDF is available here.
PDF is available here.
Abstract
We now report that a pool of Ndel1 associates directly with Dynamin 2 (Dyn2), a large cytosolic GTPase involved in the trafficking of the AMPA receptor subunit GluR1. In vitro, Ndel1 enhances Dyn2 GTPase activity in its unassembled and assembled forms, without promoting oligomerization of the enzyme...
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PMID: 21283621
PDF is available here.
PDF is available here.
Abstract
We reported the unexpected proteomic identification in endothelial cells (EC) of Myoferlin, a member of the Ferlin family of transmembrane proteins. Ferlins are well known to regulate the fusion of lipid vesicles at the plasma membrane in muscle cells, and we showed that Myoferlin gene knockdown not...
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PMID: 21586340
PDF is available here.
PDF is available here.
Abstract
We reported that the yeast Sc (strain CNCM I-3856) modulates transcript and protein expressions involved in inflammation, recruitment and activation of immune cells in differentiated porcine intestinal epithelial IPEC-1 cells. We demonstrated that viable Sc inhibits the ETEC-induced expression of pr...
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PMID: 21483702
PDF is available here.
PDF is available here.
Abstract
We determined the complete genomic sequence of H. parasuis SH0165, a highly virulent strain of serovar 5, which was isolated from a hog pen in North China. The single circular chromosome was 2,269,156 base pairs in length and contained 2,031 protein-coding genes. Together with the full spectrum of g...
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PMID: 21611187
PDF is available here.
PDF is available here.
Science Signaling (4)177 2011
Abstract
The ability to coordinate membrane trafficking events in space and time is of fundamental importance in biology. Polarized trafficking is essential for processes as diverse as cytokinesis, regulated endocytosis, cell motility, and morphogenesis, yet the interface between cellular sig...
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PMID: 21673314
PDF is available here.
PDF is available here.
Abstract
The hexosamine biosynthesis pathway (HBP) flux and protein O-linked N-acetyl-glucosamine (O-GlcNAc) levels have been implicated in mediating the adverse effects of diabetes in the cardiovascular system. Activation of these pathways with glucosamine has been shown to mimic some of the...
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PMID: 21494549
PDF is available here.
PDF is available here.
Abstract
We attempted to identify cell surface markers for cardiomyocytes derived from hESC/hiPSCs.
We adopted a previously reported differentiation protocol for hESCs based on high density monolayer culture to hiPSCs with some modification. Cardiac troponin-T (TNNT2)-positive cardiomyocytes...
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PMID: 21876760
PDF is available here.
PDF is available here.
Abstract
We focus on Nedd4 and Nedd4-2 ubiquitin ligases and the mechanisms by which they regulate voltage-gated ion channels and describe a novel paradigm on the mechanisms that underpin aberrant ion channel function in neurological disorders....
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PMID: 21057195
PDF is available here.
PDF is available here.
Abstract
We report a proteomic study of the mitotic spindle from Chinese Hamster Ovary (CHO) cells. Four different isolations of metaphase spindles were subjected to Multi-dimensional Protein Identification Technology (MudPIT) analysis and tandem mass spectrometry. We identified 1155 proteins and used Gene O...
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PMID: 21647379
PDF is available here.
PDF is available here.
Abstract
We exploited the PI3K/Akt dependent cytoprotective effect of Tat-expressing CHME5 cells. We screened a collection of compounds known to modulate inflammation, and identified three novel compounds: Lancemaside A, Compound K and Arctigenin that abolished the cytoprotective phenotype of Tat-expressing...
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PMID: 21765914
PDF is available here.
PDF is available here.
Abstract
Pseudomonas aeruginosa produces a quorum sensing molecule termed the Pseudomonas Quinolone Signal (2-heptyl-3-hydroxy-4-quinolone; PQS) that regulates an array of genes involved in virulence. This chapter addresses four related techniques useful for detecting and quantifying PQS. First, extraction o...
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PMID: 21031314
PDF is available here.
PDF is available here.
Abstract
We describe a method to prepare photosystem I (PSI) submembrane fractions derived from the chloroplast stroma lamellae of spinach chloroplasts. These preparations retain the cytochrome b6/f complex and a pool of about 11 plastoquinones per P700. The PSI submembrane fractions are thus able to perform...
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PMID: 20960117
PDF is available here.
PDF is available here.
Abstract
We describe methods to isolate highly active oxygen-evolving photosystem II (PSII) membranes and core complexes from higher plants, and to purify subunits of the oxygen-evolving complex (OEC). The membrane samples used as the material for various in vitro studies of PSII are prepared by solubilizing...
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PMID: 20960116
PDF is available here.
PDF is available here.
Abstract
Methods to isolate and purify 6- and 5-Chl D1/D2/Cyt b559 photosystem II (PSII) reaction center (RC) complexes from plants are presented, and the advantages and disadvantages of each procedure are discussed. One of the simpler 6-Chl procedures and a procedure for isolating 5-Chl complexes are descri...
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PMID: 20960118
PDF is available here.
PDF is available here.
Journal of Membrane Biology (239)1-2 2011
Abstract
Abstract
We have shown an effective recovery from arsenic-induced death signaling in erythrocytes in response to treatment with atorvastatin (ATV) and N-acetyl cysteine (NAC) in rats. Our results emphasized on the importance of cholesterol in the promotion of ROS-mediated Fas signaling in red cells. Arsenic-...
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PMID: 20946910
PDF is available here.
PDF is available here.
Biochemical Journal (433)2 2010
Abstract
We truncated polycystin-2 within the predicted loops 1-5 and tagged the constructs at the C-terminus with an HA (haemagglutinin) epitope. After transient expression and selective membrane permeabilization, immunofluorescence staining for the HA epitope revealed that loops 1, 3 and 5 extend into the...
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PMID: 21044049
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)3 2011
Abstract
We show that H4IIE liver cells express all three types of IP₃ receptor, but only type 1 and to a lesser extent type 3, but not type 2, participate in the activation of CRAC current (I(CRAC)), while type 1 and type 2, but not type 3, participate in observed Ca²+ release in response to receptor sti...
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PMID: 21182823
PDF is available here.
PDF is available here.
Abstract
We found that there were few biochemical differences in post-translational modifications between membrane-anchored and soluble forms of CR-1. Flow cytometric analysis revealed an intercellular transfer of the membrane-bound form of CR-1 between cells. CR-1-expressing cells formed unique membrane ext...
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PMID: 21055389
PDF is available here.
PDF is available here.
Abstract
Despite the capacity of chaperones and other homeostatic components to restore folding equilibrium, cells appear poorly adapted for chronic oxidative stress that increases in cancer and in metabolic and neurodegenerative diseases. Modulation of endogenous cellular defense mechanisms...
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PMID: 20446769
PDF is available here.
PDF is available here.
Abstract
Mono-dispersed SiO(2)-CaO bioactive glass nanospheres (BGNS) were prepared by a two step sol-gel method in the absence of surfactant. The size of BGNS ranged from 200 to 350 nm in diameter and exhibited a rough surface texture. In vitro biomineralization tests showed that BGNS could...
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PMID: 20725980
PDF is available here.
PDF is available here.
Molecular BioSystems (6)12 2010
Abstract
We approached this subject in a syngeneic mouse model of ovarian cancer. Antibody microarrays were applied to obtain the proteomic profiles of IF5 and IG10, two spontaneously transformed mouse ovarian surface epithelial (MOSE) cell lines of cognate clonal origin but different tumorigenic behavior in...
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PMID: 20938528
PDF is available here.
PDF is available here.
Abstract
We examined the effects of ASL volume expansion on ENaC activity in primary human bronchial epithelial (HBE) cell cultures. We found that ENaC activity increases dramatically after rapid dilution of endogenous ASL. Approximately 35% of the increase in Na(+) absorption was attributable to activation...
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PMID: 20097829
PDF is available here.
PDF is available here.
British Journal of Nutrition (104)12 2010
Abstract
Dietary fish oil modulates skeletal muscle membrane fatty acid composition. Similar changes in heart membrane composition modulate myocardial oxygen consumption and enhance mechanical performance. The rat in vivo autologous perfused hindlimb was used to investigate the influence of membrane composit...
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PMID: 20691135
PDF is available here.
PDF is available here.
Abstract
We used three-dimensional super-resolution fluorescence microscopy (interferometric photoactivated localization microscopy) to map nanoscale protein organization in focal adhesions. Our results reveal that integrins and actin are vertically separated by a ∼40-nm focal adhesion core region consisti...
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PMID: 21107430
PDF is available here.
PDF is available here.
EMBO Journal (29)23 2010
Abstract
We report the structure of a Ca(2+)/CaM Ca(V)1.2 C-terminal tail complex that contains two PreIQ helices bridged by two Ca(2+)/CaMs and two Ca(2+)/CaM-IQ domain complexes. Sedimentation equilibrium experiments establish that the complex has a 2:1 Ca(2+)/CaM:C-terminal tail stoichiometry and does not...
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PMID: 20953164
PDF is available here.
PDF is available here.