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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Cytoplasm (2):
Recent article
Cytoplasm
Abstract
We examined the effect of Cav-1 on IGFBP-5 internalization, trafficking and secretion. We demonstrated that IGFBP-5 localized to lipid rafts in human lung fibroblasts and bound Cav-1. Cav-1 was detected in the nucleus in IGFBP-5-expressing fibroblasts, within aggregates enriched with IGFBP-5, sugges...
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PMID: 20345844
PDF is available here.
PDF is available here.
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Proteomics (11)7 2011
Abstract
We report the subproteome reference maps of E. coli B REL606 by analyzing cytoplasmic, periplasmic, inner and outer membrane, and extracellular proteomes based on the genome information using experimental and computational approaches. Among the total of 3487 spots, 651 proteins including 410 non-red...
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PMID: 21337514
PDF is available here.
PDF is available here.
PLoS Biology (9)4 2011
Abstract
We exploit a yeast model and purified FUS to elucidate mechanisms of FUS aggregation and toxicity. Like TDP-43, FUS must aggregate in the cytoplasm and bind RNA to confer toxicity in yeast. These cytoplasmic FUS aggregates partition to stress granule compartments just as they do in ALS patients. Imp...
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PMID: 21541367
PDF is available here.
PDF is available here.
PLoS Biology (9)4 2011
Abstract
We report a yeast model of human FUS/TLS expression that recapitulates multiple salient features of the pathology of the disease-causing mutant proteins, including nuclear to cytoplasmic translocation, inclusion formation, and cytotoxicity. Protein domain analysis indicates that the carboxyl-terminu...
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PMID: 21541368
PDF is available here.
PDF is available here.
Abstract
We identify a previously uncharacterized type of bacterial communication mediated by nanotubes that bridge neighboring cells. Using Bacillus subtilis as a model organism, we visualized transfer of cytoplasmic fluorescent molecules between adjacent cells. Additionally, by coculturing strains harborin...
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PMID: 21335240
PDF is available here.
PDF is available here.
Abstract
FlhB is a key protein in the regulation of protein export by the bacterial flagellar secretion system. It is composed of two domains: an N-terminal transmembrane domain and a C-terminal cytoplasmic domain (FlhBc). Here, the crystallization and preliminary crystallographic analysis of FlhBc from Aqui...
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PMID: 21301106
PDF is available here.
PDF is available here.
Abstract
Islet amyloid polypeptide is originally isolated as the chief constituent of amyloid deposits in type 2 diabetic islets. Islet amyloid polypeptide hyposecretion was known in type 1 diabetics and this study aimed to detect possibly reduced islet amyloid polypeptide-positive cells in t...
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PMID: 21633185
PDF is available here.
PDF is available here.
Abstract
A growing number of research consortia are now focused on generating antibodies and recombinant antibody fragments that target the human proteome. A particularly valuable application for these binding molecules would be their use inside a living cell, e.g., for imaging or functional...
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PMID: 21099369
PDF is available here.
PDF is available here.
Abstract
We isolate and characterize the human protein named Rep8 or Ubxd6 as a new cofactor of p97. Mouse Rep8 is highly tissue-specific and abundant in gonads. In testes, Rep8 is expressed in post-meiotic round spermatids, whereas in ovaries Rep8 is expressed in granulosa cells. Rep8 associates directly wi...
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PMID: 21949850
PDF is available here.
PDF is available here.
Comptes Rendus Biologies (334)8-9 2011
Abstract
Genetic code alterations discovered over the last 40 years in bacteria and eukaryotes invalidate the hypothesis that the code is universal and frozen. Mitochondria of various yeast species translate the UGA stop codon as tryptophan (Trp) and leucine (Leu) CUN codons (N = any nucleoti...
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PMID: 21819941
PDF is available here.
PDF is available here.
Abstract
We estimate the net selective effect of Wolbachia on its host in a laboratory-adapted population of D. melanogaster, to determine the mechanisms leading to its persistence in the laboratory environment. We found i) no significant effects of Wolbachia infection on female egg-to-adult survival or adul...
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PMID: 21283625
PDF is available here.
PDF is available here.
Abstract
Establishing a diagnosis of ovarian clear cell carcinoma (O-CCC) can be subject to significant interobserver variation. Accurately diagnosing this tumor is important because of its chemoresistance and reported association with Lynch syndrome. The spectrum of the morphologic features of O-CCC has not...
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PMID: 21164285
PDF is available here.
PDF is available here.
Trends in Cell Biology (21)1 2011
Abstract
I argue that studying these specialized systems will help to reveal the basis for fungal diversity and provide general principles for co-evolution of organelles and multicellular complexity.
Copyright © 2010 Elsevier Ltd. All rights reserved....
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PMID: 20888233
PDF is available here.
PDF is available here.
Biochemical Journal (433)2 2010
Abstract
AE1 (anion exchanger 1) and protein 4.2 associate in a protein complex bridging the erythrocyte membrane and cytoskeleton; disruption of the complex results in unstable erythrocytes and HS (hereditary spherocytosis). Three HS mutations (E40K, G130R and P327R) in cdAE1 (the cytoplasmic domain of AE1)...
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PMID: 21039340
PDF is available here.
PDF is available here.
Clinical Nephrology (74)6 2010
Abstract
A family was identified with autosomal dominant inheritance of anemia, polyuria, hyperuricemia, and chronic kidney disease. Mutational analysis revealed a novel heterozygous mutation c.58T > C resulting in the amino acid substitution of cysteine for arginine in the preprorenin signal sequence (p.cys...
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PMID: 21084044
PDF is available here.
PDF is available here.
Abstract
We identified a novel interaction in human osteoclast-like cells between SQSTM1 and Autophagy-Linked FYVE domain-containing protein (ALFY/WDFY3). Endogenous ALFY and SQSTM1 both localised within the nuclei of osteoclasts and their mononuclear precursors. When osteoclasts were starved to induce autop...
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PMID: 20971078
PDF is available here.
PDF is available here.
DNA Repair (9)11 2010
Abstract
We have previously shown that whereas T-cells from normal individuals undergo accumulation of p53 and apoptosis when treated with the genotoxic agent Actinomycin D (ActD), those from Ataxia Telangiectasia (AT) and Nijmegen Breakage Syndrome (NBS) patients resist ActD-induced apoptosis [1]. We have n...
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PMID: 20947454
PDF is available here.
PDF is available here.
Abstract
Abstract
We examined 75 vascular lesions: KHE (n=18), TA (n=13), infantile hemangioma (n=13), pyogenic granuloma (n=18), and granulation tissue (n=13). Overall, KHE and TA shared an identical endothelial immunophenotype: the neoplastic spindle cells were Prox1, podoplanin, LYVE-1, CD31, and CD34, whereas end...
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PMID: 20975337
PDF is available here.
PDF is available here.
Cell Biology International (34)11 2010
Abstract
The 'microtrabecular lattice' (MTL) that Keith Porter described in the 1970s and 1980s is reconsidered as a proposed fundamental cytoplasmic structure of eukaryotic cells. Although considered to be an artefact by most cell biologists of his time (and probably ours), the case is made that something l...
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PMID: 20939832
PDF is available here.
PDF is available here.
EMBO Journal (29)21 2010
Abstract
We show here that EI and HPr localize near the Escherichia coli cell poles. Polar localization of each protein occurs independently, but HPr is released from the poles in an EI- and sugar-dependent manner. Conversely, the β-glucoside-specific permease, BglF, localizes to the cell membrane. EI, HPr...
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PMID: 20924357
PDF is available here.
PDF is available here.
Abstract
We examined possible mechanism(s) responsible for the great reduction in diffusion constants of macromolecules in vivo from that at infinite dilution. In an Escherichia coli cytoplasm model comprised of 15 different macromolecule types at physiological concentrations, BD simulations of molecular-sha...
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PMID: 20937902
PDF is available here.
PDF is available here.
Abstract
We present a role for p21-activated kinase 4 (Pak4) in ovarian cancer progression. We show a significant association between increased expression of Pak4 and its activated form, phosphorylated (p)-Pak4 Ser(474), with metastasis of ovarian cancers, shorter overall and disease-free survival, advanced...
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PMID: 20926745
PDF is available here.
PDF is available here.
Biochemical Journal (432)1 2010
Abstract
We report on the properties of a series of site-directed mutants of two adjacent tyrosine residues involved in iron co-ordination, and demonstrate that, in contrast with mutation of equivalent residues in the N-lobe of human transferrin, the mutant FbpAs retain significant iron-binding affinity rega...
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PMID: 20799927
PDF is available here.
PDF is available here.
Abstract
A Gram-negative, motile, non-spore-forming bacterial strain, designated HU1-GD12(T), was isolated from freshwater sediment. The strain was characterized by using a polyphasic approach in order to determine its taxonomic position. Comparative analysis of the 16S rRNA gene sequence showed that the iso...
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PMID: 19946046
PDF is available here.
PDF is available here.
Human Pathology (41)10 2010
Abstract
Desmocollin 2, a desmosomal component, is a key membrane glycoprotein critically involved in cell-cell adhesion and the maintenance of normal tissue architectures in epithelia. Reports exploring the link of desmocollin expression to cancers are limited. The aim of this study was to investigate the e...
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PMID: 20621329
PDF is available here.
PDF is available here.
Human Pathology (41)10 2010
Abstract
We analyzed CKS-1B expression in mantle cell lymphoma cell lines and tumors by Western blot and immunohistochemical analysis. In 4 mantle cell lymphoma cell lines, CKS-1B was expressed at variable levels and correlated inversely with p27 expression. In mantle cell lymphoma tumors, CKS-1B was positiv...
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PMID: 20688354
PDF is available here.
PDF is available here.
Anticancer Research (30)10 2010
Abstract
Induction of angiogenic responses by multiple factors, a crucial step in tumor growth and metastasis, is not completely understood. Recently, involvement of the cytoskeletal actin-binding proteins in angiogenesis has been suggested as a target for anti-neovascular cancer therapy in vitro. In this st...
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PMID: 21036706
PDF is available here.
PDF is available here.
Abstract
We have shown that one key determinant of cisplatin-resistance in testicular embryonal carcinoma (EC) is high cytoplasmic expression of the cyclin-dependent kinase (CDK) inhibitor p21. The EC component of the majority of refractory testicular cancer patients exhibited high cytoplasmic p21 expression...
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PMID: 20811155
PDF is available here.
PDF is available here.
Abstract
We searched nuclear JAK2 in total bone marrow (BM) cells and in 4 sorted BM cell populations (CD34(+), CD15(+), CD41(+), and CD71(+)) of 10 myeloproliferative neoplasia (MPN) patients with JAK2V617F mutation and 5 patients with wild-type JAK2 MPN. Confocal immunofluorescent images and Western blot a...
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PMID: 20861460
PDF is available here.
PDF is available here.
Kidney International (78)10 2010
Abstract
Abstract
We fractionated the cytoplasm of HCT116 colon cancer cells and used a proteomic approach to analyze cytoplasmic HMGB1-binding proteins. Pull-down experiments using recombinant HMGB1 protein as bait, followed by mass spectrometry analysis identified 162 interacting proteins. Among them were 74 protei...
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PMID: 20812762
PDF is available here.
PDF is available here.
ISME Journal (4)9 2010
Abstract
We present a broad-ranging microscopic, molecular and microspectrophotometric analysis of uncultured P. sphagnophila collected from four field locations in Nova Scotia, Canada. Consistent with its morphological characteristics, 18S ribosomal DNA (rDNA) phylogenies indicate that P. sphagnophila is sp...
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PMID: 20376101
PDF is available here.
PDF is available here.
Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences (368)1925 2010
Abstract
Intracellular Ca(2+) dynamics provides excitation-contraction coupling in cardiac myocytes. Under pathological conditions, spontaneous Ca(2+) release events can lead to intracellular Ca(2+) travelling waves, which can break, giving transitory or persistent intracellular re-entrant Ca(2+) scroll wave...
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PMID: 20643687
PDF is available here.
PDF is available here.
Abstract
We discuss the influenza A virion structure and its replication steps in host cells....
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PMID: 20845736
PDF is available here.
PDF is available here.
Journal of Pathology (222)1 2010
Abstract
We examined the expression of delta-catenin by immunohistochemistry in 115 cases of non-small cell lung cancer (NSCLC) (including 65 cases with follow-up records and 50 cases with paired lymph node metastasis lesions). The mRNA and protein expression of delta-catenin was also detected in 30 cases of...
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PMID: 20593408
PDF is available here.
PDF is available here.
Cell Host & Microbe (8)2 2010
Abstract
We demonstrate that Sindbis virus, a prototypical single-stranded arthropod-borne alphavirus, uses U-rich 3' UTR sequences in its RNAs to recruit a known regulator of cellular mRNA stability, the HuR protein, during infections of both human and vector mosquito cells. HuR binds viral RNAs with high s...
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PMID: 20709296
PDF is available here.
PDF is available here.
Developmental Biology (344)2 2010
Abstract
We showed that pam-1 mutants fail to polarize the A-P axis. Here we show that PAM-1 can be found in mature sperm and in cytoplasm throughout early embryogenesis where it concentrates around mitotic centrosomes and chromosomes. We provide further evidence that PAM-1 acts early in the polarization pro...
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PMID: 20599902
PDF is available here.
PDF is available here.
Cellular Reprogramming (12)4 2010
Abstract
We compare two different SCNT protocols with TSA and explore, for the first time, the effect of another HDACi, valproic acid (VPA), on the in vitro development, blastocyst quality, and full-term development of mouse B6CBAF1 cloned embryos. Rates of blastocyst development in SCNT embryos treated with...
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PMID: 20698782
PDF is available here.
PDF is available here.
Cellular Reprogramming (12)4 2010
Abstract
Universal recipients in the G2 phase of mitotic cell cycle (preactivated oocytes, zygotes, blastomeres) accept embryonic nuclei in all the stages of their cell cycle. To test if recipients in the G2 of meiotic cycle (immature oocytes) are universal recipients, mouse germinal vesicle (GV) oocytes wer...
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PMID: 20698781
PDF is available here.
PDF is available here.
Abstract
We investigated MTA1 expression and its prognostic value for NSCLC.
NSCLC surgical tissue samples were taken from 100 patients with NSCLC who had been followed up for more than 2 years. The expression of MTA1 protein was evaluated by immunohistochemistry, and the correlations between the expression...
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PMID: 20661085
PDF is available here.
PDF is available here.
Abstract
We propose a post-transcriptional regulon model that explains hnRNP E1's widespread functional diversity....
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PMID: 20584894
PDF is available here.
PDF is available here.
Nature Cell Biology (12)8 2010
Abstract
Molecular Cell (39)2 2010
Abstract
We have carried out a comprehensive analysis of cytoplasmic maturation, ordering the known steps into a coherent pathway. Maturation is initiated by the ATPase Drg1. Downstream, assembly of the ribosome stalk is essential for the release of Tif6. The stalk recruits GTPases during translation. Becaus...
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PMID: 20670889
PDF is available here.
PDF is available here.
Abstract
Our studies using RNA cRACE (circularization-based rapid amplification of cDNA ends) techniques indicate that mRNA decapping in the fission yeast Schizosaccharomyces pombe often does not require prior deadenylation. Furthermore, the poly(A) polymerase-related, cytoplasmic enzyme Cid1 catalyses uridy...
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PMID: 20659020
PDF is available here.
PDF is available here.
Abstract
We report on our study in an alveolar epithelial cell line of the molecular organization of P2X7R and P2X4R receptors and the effect of their knockdown. Native gel electrophoresis reveals three P2X7R complexes of approximately 430, approximately 580 and approximately 760 kDa. The latter two correspo...
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PMID: 20405163
PDF is available here.
PDF is available here.
Abstract
Oncology Reports (24)2 2010
Abstract
CD133 antigen has been used to identify cancer stem cells in several solid tumor types, including hepatocellular carcinomas (HCCs). The aim of this study was to investigate whether the expression and subcellular localization of CD133 correlated with the clinicopathological factors, recurrence, and s...
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PMID: 20596644
PDF is available here.
PDF is available here.
Abstract
Calcium plays a major role in intracellular signaling mechanisms during ischemia reperfusion (I/R) injury of a liver cell. Under ischemic conditions, the absence of oxygen arrests oxidative phosphorylation, thereby eliminating the energy source by which hepatocellular mechanisms maintain homeostasis...
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PMID: 20690849
PDF is available here.
PDF is available here.
Abstract
We shall try to highlight the different molecular cues involved in septum formation in yeast, from the initial site selection to the final action of hydrolytic enzymes that produce cell separation.
Copyright (c) 2010 John Wiley & Sons, Ltd....
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PMID: 20641019
PDF is available here.
PDF is available here.
Abstract
Met, the hepatocyte growth factor receptor, is important in transducing signals for tumour growth and metastasis. The aim of this study was to examine the pattern of Met expression and its value as a prognostic factor in oral squamous cell carcinomas (OSCCs). The material consisted of 53 OSCCs and f...
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PMID: 20662906
PDF is available here.
PDF is available here.
Abstract
We followed visible light (VL)-induced hydroxyl radicals in various cell types and cellular sites using the electron paramagnetic resonance (EPR) spin-trapping technique.
Fibroblasts, sperm cells, cardiomyocytes, and skeletal muscle cells were irradiated with broadband (400-800 nm) VL. To detect ROS...
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PMID: 20662023
PDF is available here.
PDF is available here.
Biology of the Cell (102)8 2010
Abstract
We have determined the intracellular localization of the protein components of the 42S particle by expressing fluorescent protein-tagged fusions in transparent previtellogenic oocytes. 42Sp50 and its isoforms (EF1A-S and EF1A-O) were excluded from the nuclei and distributed uniformly throughout the...
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PMID: 20415665
PDF is available here.
PDF is available here.
Abstract
We made use of Redoxfluor to measure the redox states at the subcellular level in yeast and Chinese hamster ovary (CHO) cells. In wild-type peroxisomes harboring an intact fatty acid beta-oxidation system, the redox state within the peroxisomes was more reductive than that in the cytosol, despite th...
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PMID: 20498274
PDF is available here.
PDF is available here.
Abstract
Abstract
Infection and Immunity (78)8 2010
Abstract
We have identified several loci that are required for C. jejuni efficient entry and survival within epithelial cells. Among these loci, insertional mutations in aspA, aspB, and sodB resulted in drastic reduction in C. jejuni entry and/or survival within host cells and a severe defect in colonization...
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PMID: 20515930
PDF is available here.
PDF is available here.
Journal of Virology (84)16 2010
Abstract
We performed random mutagenesis of PrV pUL36 by transposon-mediated insertion of a 15-bp linker. By this approach, 26 pUL36 insertion mutants were selected and tested in transient transfection assays for their ability to complement one-step growth and/or viral spread of a PrV UL36 null mutant. Ten i...
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PMID: 20534865
PDF is available here.
PDF is available here.
Applied Optics (49)22 2010
Abstract
In problems relating to light propagation in biomedical tissues, the tissue is generally modeled as a turbid medium and Monte Carlo (MC) simulation is employed to compute quantities such as diffuse reflectance, fluence, and transmittance. Two prescriptions are available in the literature for MC simu...
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PMID: 20676167
PDF is available here.
PDF is available here.
Brain research (1345) 2010
Abstract
We investigated the distribution of Kv3.3-immunoreactivity in the vestibular nuclear complex of rats with particular focus on the medial vestibular nucleus (MVN). Kv3.3-immunoreactivity was widespread in the vestibular nuclei and was detected in somata, dendrites and synaptic terminals. Kv3.3-immuno...
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PMID: 20471378
PDF is available here.
PDF is available here.