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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Intracellular Space (3):
Recent article
Intracellular Space
PLoS Genetics (7)6 2011
Abstract
We map Sirt6 occupancy on mouse promoters genome-wide and show that Sirt6 occupancy is highly dynamic in response to TNF-α. More than half of Sirt6 target genes are only revealed upon stress-signaling. The majority of genes bound by NF-κB subunit RelA recruit Sirt6, and dynamic Sirt6 relocalizatio...
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PMID: 21738489
PDF is available here.
PDF is available here.
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Abstract
The Golgi apparatus in mammalian cells is positioned near the centrosome-based microtubule-organizing center (Fig. 1). Secretory cargo moves inward in membrane carriers for delivery to Golgi membranes in which it is processed and packaged for transport outward to the plasma membrane...
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PMID: 21504874
PDF is available here.
PDF is available here.
Abstract
We examined the effect of Cav-1 on IGFBP-5 internalization, trafficking and secretion. We demonstrated that IGFBP-5 localized to lipid rafts in human lung fibroblasts and bound Cav-1. Cav-1 was detected in the nucleus in IGFBP-5-expressing fibroblasts, within aggregates enriched with IGFBP-5, sugges...
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PMID: 20345844
PDF is available here.
PDF is available here.
Molecular Therapy (19)3 2011
Controlled extracellular matrix degradation in breast cancer tumors improves therapy by trastuzumab.
Abstract
We observed colocalization of ECM proteins and Her2/neu, a tumor-associated antigen that is the target for the widely used monoclonal antibody trastuzumab (Herceptin). We tested whether intratumoral expression of the peptide hormone relaxin (Rlx) would result in ECM degradation and the improvement o...
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PMID: 21081901
PDF is available here.
PDF is available here.
Abstract
We have developed a set of doubly-fluorophore labeled RNAs (more precisely, RNA/DNA chimeras) to probe in real-time the stability of siRNAs and related molecules by fluorescence resonance energy transfer (FRET). We find that these RNA probes are substrates for relevant cellular degradative processes...
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PMID: 21647381
PDF is available here.
PDF is available here.
Abstract
We previously demonstrated that, in response to B. pseudomallei infection of macrophage cell line RAW 264.7, a subset of bacteria co-localized with the autophagy marker protein, microtubule-associated protein light chain 3 (LC3), implicating autophagy in host cell defence against infection. Recent r...
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PMID: 21412437
PDF is available here.
PDF is available here.
Acta Biomaterialia (7)1 2011
Abstract
Silver nanoparticles (Ag-NP) are widely used due to their well-known antibacterial effects. In medicine Ag-NP have found applications as wound dressings, surgical instruments and bone substitute biomaterials, e.g. silver-containing calcium phosphate cements. Depending on the coating...
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PMID: 20709196
PDF is available here.
PDF is available here.
Acta Biomaterialia (7)1 2011
Abstract
A degradable, polar, hydrophobic, ionic polyurethane (D-PHI), with physical properties comparable to those of peripheral arterial vascular tissue, was evaluated for monocyte interactions with two different physical forms: two-dimensional films and three-dimensional porous scaffolds....
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PMID: 20728587
PDF is available here.
PDF is available here.
The Cancer Journal (17)2 2011
Abstract
We describe different adaptation, mostly at the level of the proteome, which cancer cells use to develop resistance to cancer treatment....
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PMID: 21427552
PDF is available here.
PDF is available here.
Abstract
We demonstrated that supernatants isolated from leukapheresis products of granulocyte-colony stimulating factor (G-CSF)-mobilized patients primed the homing of hematopoietic stem/progenitor cells (HSPC) by enhancing their chemotactic responses to stromal cell-derived factor (SDF)-1 and stimulating m...
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PMID: 20629539
PDF is available here.
PDF is available here.
Biochemical Journal (433)2 2010
Abstract
We truncated polycystin-2 within the predicted loops 1-5 and tagged the constructs at the C-terminus with an HA (haemagglutinin) epitope. After transient expression and selective membrane permeabilization, immunofluorescence staining for the HA epitope revealed that loops 1, 3 and 5 extend into the...
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PMID: 21044049
PDF is available here.
PDF is available here.
Abstract
The health of organisms and cells depends on appropriate responses to diverse internal and external cues, stimuli, or challenges, such as changes in hormone or cytokine levels, or exposure to a pathogen. Cellular responses must be tailored to the identity and intensity of the stimulus and therefore...
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PMID: 20956081
PDF is available here.
PDF is available here.
FASEB Journal (24)11 2010
Abstract
We investigated the regulation of Cx43-hemichannel activity by actomyosin contractility and intracellular [Ca(2+)] ([Ca(2+)](i)) using plasma membrane-permeable TAT peptides (100 μM) designed to interfere with interactions between the cytoplasmic loop (CL) and carboxy-terminal (CT) in primary bovin...
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PMID: 20634352
PDF is available here.
PDF is available here.
Abstract
We propose a reconsideration of the dogma that endocannabinoids are exclusively synthesized and released 'on demand', and suggest that their metabolic control is complemented by intracellular trafficking and storage in specific reservoirs.
Copyright © 2010 Elsevier Ltd. All rights reserved....
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PMID: 20570522
PDF is available here.
PDF is available here.
Biochemical Journal (432)1 2010
Abstract
We have previously identified the PKC (protein kinase C)-anchoring protein RACK1 (receptor for activated C-kinase 1), as a specific binding partner for the cAMP-specific phosphodiesterase PDE4D5, suggesting a potential site for cross-talk between the PKC and cAMP signalling pathways. In the present...
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PMID: 20819076
PDF is available here.
PDF is available here.
Abstract
We screened a human fetal cDNA library for interacting proteins and identified Grb10 as an interactor. This interaction was verified by co-immunoprecipitation and intracellular co-localization studies. The potential segment of Bim L that binds Grb10 was identified via a yeast mating test. Grb10 inte...
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PMID: 20174874
PDF is available here.
PDF is available here.
Neuroscience Letters (482)2 2010
Abstract
I by rotenone was found to cause intensive death of cultured cerebellar granule neurons (CGNs) that was preceded by an increase in intracellular calcium concentration ([Ca(2+)](i)). The neuronal death induced by rotenone was significantly potentiated by glutamine. In addition, inhibition of Na/K-ATP...
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PMID: 20650305
PDF is available here.
PDF is available here.
Biochemical Journal (431)2 2010
Abstract
These results reiterate the unique features of this group of kinases and present an opportunity for designing more specific antiviral compounds....
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PMID: 20704565
PDF is available here.
PDF is available here.
Abstract
Atheroprone regions of the arterial circulation are characterized by time-varying, reversing, and oscillatory wall shear stress. Several in vivo and in vitro studies have demonstrated that flow reversal (retrograde flow) is atherogenic and proinflammatory. The molecular and structural basis for the...
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PMID: 20554908
PDF is available here.
PDF is available here.
Abstract
We reported the synthesis and evaluation of anti-Toxoplasma gondii and antimicrobial activities in vitro of three new compound series obtained from ethyl(5-methyl-1-H-imidazole-4-carboxylate): acylthiosemicarbazide analogues 3a-d, 4-thiazolidinone analogues 4a-d and 1,3,4-thiadiazole analogues 5a-d....
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PMID: 20541294
PDF is available here.
PDF is available here.
The composition of the polyglutamine-containing proteins influences their co-aggregation properties.
Abstract
We analysed the co-aggregation properties of the N-terminal fragment of huntingtin fused with unrelated non-aggregating and/or self-aggregating peptides. We show that the ability of the mutated N-terminal huntingtin fragment to sequester non-related proteins can be significantly increased by fusion...
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PMID: 20515443
PDF is available here.
PDF is available here.
Abstract
The extraction conditions of intracellular polysaccharide (IPS) from Pleurotus sp. mycelium in submerged culture were investigated. Four parameters affecting the IPS extraction, ultrasonic treatment time, extraction temperature, extraction time and ethanol concentration, were determined by single fa...
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PMID: 20580645
PDF is available here.
PDF is available here.
Abstract
We have established a strategy to produce and purify recombinant farnesylated guanylate-binding protein 1 (hGBP1), a dynamin-related large GTPase. Our system is based on the coexpression of hGBP1 with the two subunits of human farnesyltransferase in Escherichia coli and a chromatographic separation...
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PMID: 20348589
PDF is available here.
PDF is available here.
Abstract
Extracellular adenosine disrupted mitochondrial membrane potentials in HuH-7 cells, a Fas-deficient human hepatoma cell line, and the effect was inhibited by the adenosine transporter inhibitor dipyridamole or by overexpressing Bcl-X(L). Adenosine downregulated the expression of mRNAs and proteins f...
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PMID: 20063052
PDF is available here.
PDF is available here.
Abstract
Our results for the first time show that antiproliferative effects of plumbagin are mediated by modulation of cellular redox. These results provide a rationale for application of thiol-depleting agents as anti-inflammatory drugs.
Published 2010 Wiley-Liss, Inc....
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PMID: 20564204
PDF is available here.
PDF is available here.
Abstract
We investigated whether increased FPN1 and/or HP expression could attenuate iron-induced oxidative stress in the dopaminergic MES23.5 cell line. We generated MES23.5 cells with stable overexpression of FPN1 and/or HP. Our study showed that overexpression of FPN1 and/or HP increased iron efflux, lowe...
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PMID: 20564203
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (135)8 2010
Abstract
We critically summarise the state of the art in intracellular magnesium detection by fluorescent probes and focus on the need for improving methods and techniques in this area. We highlight the advantages of last-generation fluorescent indicators and discuss a number of challenges and opportunities...
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PMID: 20544083
PDF is available here.
PDF is available here.
Abstract
We established a screening system searching for mRNA processing factors in human cells by combining the luciferase reporter system and fluorescence in situ hybridization, which evaluates the nuclear/cytoplasmic distribution of bulk poly(A)+ RNA. This system makes it possible to search for the compou...
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PMID: 20622428
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)30 2010
Abstract
We found that p94/calpain 3, a skeletal-muscle-specific member of the Ca(2+)-activated intracellular "modulator proteases" that is responsible for a limb-girdle muscular dystrophy ("calpainopathy"), underwent Na(+)-dependent, but not Cs(+)-dependent, autolysis in the absence of Ca(2+). Furthermore,...
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PMID: 20460380
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)30 2010
Abstract
This study examined the effect of H(2)O(2) on the TRPC6 channel and its underlying mechanisms using a TRPC6 heterologous expression system. In TRPC6-expressing HEK293T cells, H(2)O(2) significantly stimulated Ca(2+) entry in a dose-dependent manner. Electrophysiological experiments showed that H(2)O...
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PMID: 20501650
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)30 2010
Abstract
We report the crystal structure of a complex between the Pseudomonas translocator chaperone PcrH and a short region from the minor translocator PopD. PcrH displays a 7-helical tetratricopeptide repeat fold that harbors the PopD peptide within its concave region, originally believed to be involved in...
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PMID: 20385547
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)30 2010
Abstract
We show that the steep [K(+)](o) dependence of Kir2.1-mediated outward I(K1) was due to [K(+)](o)-dependent inhibition of outward I(K1) by extracellular Na(+) and Ca(2+). This could be accounted for by Na(+)/Ca(2+) inhibition of I(K1) through screening of local negative surface charges. Consistent w...
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PMID: 20495007
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)29 2010
Abstract
We assessed the possibility that angiotensin-1 (AT1R) or angiotensin-2 (AT2R) receptors on the nuclear envelope mediate effects on cardiomyocyte gene expression. Immunoblots of nucleus-enriched fractions from isolated cardiomyocytes indicated the presence of AT1R and AT2R proteins that copurified wi...
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PMID: 20463030
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)29 2010
Abstract
We demonstrate that IL-2Rbeta is cleaved in cell lines of different origin, including T cells, generating an intracellular 37-kDa fragment (37beta ic) that comprises the full intracellular C-terminal and transmembrane domains. Ectodomain shedding of IL-2Rbeta decreases in a mutant deleted of the jux...
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PMID: 20495002
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)29 2010
Abstract
We demonstrated directly that iron availability is important to the action of antibiotics, and the ferric reductases of Pseudomonas putida and Pseudomonas aeruginosa could accelerate antibiotic-mediated cell death by promoting the Fenton reaction. The modulation of reduced nicotinamide-adenine dinuc...
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PMID: 20479007
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)29 2010
Abstract
We show here that forced expression of xCT, the substrate-specific subunit of the cystine/glutamate antiporter, in gamma-glutamylcysteine synthetase knock-out cells rescues GSH deficiency by increasing cellular cystine uptake, leading to augmented intracellular and surprisingly high extracellular cy...
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PMID: 20463017
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)29 2010
Abstract
We found that two host small G proteins, Rab5 and Rab7, were associated with the pathway of autophagosome formation and the fate of intracellular GAS. Rab5 was involved in bacterial invasion and endosome fusion. Rab7 was clearly multifunctional, with roles in bacterial invasion, endosome maturation,...
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PMID: 20472552
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)29 2010
Abstract
We have developed two nanoparticle formulations, cationic liposome-polycation-DNA (LPD) and anionic liposome-polycation-DNA (LPD-II), for systemic co-delivery of doxorubicin (Dox) and a therapeutic small interfering RNA (siRNA) to multiple drug resistance (MDR) tumors. In this study, we have provide...
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PMID: 20460382
PDF is available here.
PDF is available here.
Abstract
Selectivity toward cancer cells is the most desirable element in cancer therapeutics. Par-4 is a cancer cell-selective proapoptotic protein that functions intracellularly in the cytoplasmic and nuclear compartments as a tumor suppressor. Moreover, recent findings indicate that the Par-4 protein is s...
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PMID: 20440265
PDF is available here.
PDF is available here.
Plant Physiology (153)3 2010
Abstract
We conclude that GR1 plays a crucial role in daylength-dependent redox signaling and that this function cannot be replaced by the second Arabidopsis GR gene or by thiol systems such as the thioredoxin system....
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PMID: 20488891
PDF is available here.
PDF is available here.
Plant Physiology (153)3 2010
Abstract
Our results confirm that AtPAP26 is a principal contributor to Pi stress-inducible APase activity, and that it plays an important role in the Pi metabolism of -Pi Arabidopsis....
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PMID: 20348213
PDF is available here.
PDF is available here.
Environmental Research (110)5 2010
Abstract
We report the results obtained with a mu-XANES experimental setup based on Kirkpatrick-Baez X-ray focusing optics that maintains high flux of incoming radiation (>10(11)ph/s) at micrometric spatial resolution (1.5 x 4.0 microm(2)). This original experimental setup enabled the direct speciation analy...
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PMID: 19800058
PDF is available here.
PDF is available here.
Environmental Research (110)5 2010
Abstract
Arsenic is a known human carcinogen, inducing tumors of the skin, urinary bladder, liver and lung. Inorganic arsenic, existing in highly toxic trivalent and significantly less toxic pentavalent forms, is methylated to mono- and di-methylated species mainly in the liver. Due to the low toxicity of pe...
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PMID: 19758587
PDF is available here.
PDF is available here.
Cellular distribution and subcellular localization of mCLCA1/2 in murine gastrointestinal epithelia.
Abstract
We have generated a rabbit polyclonal antibody (pAb849) against aa 424-443 of mCLCA1/2. In HEK293 cells transfected with mCLCA1; pAb849 detected two specific protein bands at approximately 125 kDa and 90 kDa, representing full-length precursor and N-terminal cleavage product, respectively. pAb849 al...
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PMID: 20385786
PDF is available here.
PDF is available here.
Abstract
Since the realisation that the antigen-binding regions of antibodies, the variable (V) regions, can be uncoupled from the rest of the molecule to create fragments that recognise and abrogate particular protein functions in cells, the use of antibody fragments inside cells has become...
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PMID: 20544739
PDF is available here.
PDF is available here.
Journal of Fluorescence (20)4 2010
Abstract
Flow cytometry provides a rapid and high-content multiparameter analysis of individual microorganisms within a population. In the past years, several fluorescent stains were developed in order to monitor DNA content distribution and cell-cycle phases, mainly in eukaryotic cells. Recently, due to its...
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PMID: 20352307
PDF is available here.
PDF is available here.
Journal of Neuroscience (30)26 2010
Abstract
We explore how facial-motoneuron spikes are translated, via an intrinsic muscle, to whisker movements. This is achieved by constructing, simulating, and analyzing a computational, biomechanical model of the motor plant, and by measuring spiking to movement transformations at small and large angles u...
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PMID: 20592215
PDF is available here.
PDF is available here.
Journal of Neuroscience (30)26 2010
Abstract
We show that postsynaptic IP(3)-mediated Ca(2+) release in response to a presynaptic tetanus in an SN that induces PTP can confer transient plasticity onto a neighboring SN synapse receiving subthreshold activation. This heterosynaptic sharing of plasticity represents a dynamic, short-term synaptic...
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PMID: 20592201
PDF is available here.
PDF is available here.
Biochemistry (Washington) (49)23 2010
Abstract
We have investigated the role of aggregation in the signal transduction for dimeric forms of the prototypical GPCR rhodopsin using molecular dynamics simulations. The early steps after photoexcitation are characterized by a tandem mechanism in which one monomer is responsible for light detection whi...
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PMID: 20459069
PDF is available here.
PDF is available here.
Physical Review Letters (104)22 2010
Abstract
I demonstrate that dynamic Bayesian decision making can be implemented by an intracellular kinetics with a dual positive feedback structure. I also show that the combination of linear instantaneous and nonlinear stationary sensitivities to the input dominantly contributes to decision making efficien...
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PMID: 20867209
PDF is available here.
PDF is available here.
Abstract
We determine, through a unified electrospray ionization mass spectrometry (ESI-MS)-based strategy, in an environment that mimics the cellular redox milieu, the apparent Cu(I)-binding affinities for a representative set of intracellular copper proteins involved in enzymatic redox catalysis, in copper...
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PMID: 20463663
PDF is available here.
PDF is available here.
Abstract
We first report that a synthetic omega-alkynyl-palmitate analog can be readily and specifically incorporated into GAPDH or mitochondrial 3-hydroxyl-3-methylglutaryl-CoA synthase in vitro and reacted with an azido-biotin probe or the fluorogenic probe 3-azido-7-hydroxycoumarin using click chemistry f...
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PMID: 20028662
PDF is available here.
PDF is available here.
Abstract
The primary aim of the present study was to investigate in which cellular compartments fatty acid trans-locase CD36 (FAT/CD36) is localized. Intact and fully functional skeletal muscle mitochondria were isolated from lean and obese female Zucker rats and from 10 healthy male individuals. FAT/CD36 cou...
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PMID: 20023206
PDF is available here.
PDF is available here.
Abstract
We show that resistin, a cystein-rich protein believed to regulate carbohydrate metabolism, competes with LPS for binding to TLR4. Binding of recombinant resistin to human myeloid and epithelial cells was assessed by flow cytometry and its co-precipitation with TLR4 was demonstrated. Antibodies agai...
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PMID: 19754671
PDF is available here.
PDF is available here.
Chemical Reviews (110)5 2010
Abstract
Brain research (1330) 2010
Abstract
A major obstacle that hampers the design of drug therapy for traumatic brain injury is the incomplete understanding of the biochemical pathways that lead to secondary cellular injury and contribute to cell death. One such pathway involves reactive species that generate potentially cytotoxic zinc ion...
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PMID: 20303343
PDF is available here.
PDF is available here.
Abstract
Anthocyanic vacuolar inclusions (AVIs) are intra-vacuolar structures capable of concentrating anthocyanins and are present in over 50 of the highest anthocyanin-accumulating plant species. Presence of AVIs alters pigment intensity, total anthocyanin levels, pigment hue and causes bathochromic shifts...
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PMID: 20238125
PDF is available here.
PDF is available here.
Neuropharmacology (58)6 2010
Abstract
We attempt to organize the current research by summarizing findings affecting early neurogenesis: during proliferation, fate commitment and migration, versus late neurogenesis: including dendritic development, synaptic integration, and survival. We discuss the roles of three different classes of fac...
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PMID: 20097213
PDF is available here.
PDF is available here.
Abstract
We hypothesized that the stimulatory mechanism of oxidized phospholipids is via induction of osteoclast-regulating cytokines in osteoblasts. To investigate the effects of oxidized phospholipids on expression of such cytokines, murine calvarial preosteoblasts, MC3T3-E1, were treated with oxidized 1-p...
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PMID: 19965598
PDF is available here.
PDF is available here.
Abstract
We show novel functional modulation of GnRH signaling pathways via GPCR heterodimerization. Immunohistochemical analysis showed colocalization of R1 and R4 in test cells of the ascidian ovary. The native R1-R4 heterodimerization was detected in the Ciona ovary by coimmunoprecipitation analysis. The...
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PMID: 20026483
PDF is available here.
PDF is available here.