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Chromaffin Cells (1):
Recent article
Chromaffin Cells
Abstract
We show that the ability of sybII to support exocytosis is inhibited by addition of one or two residues to the sybII C terminus depending on their energy of transfer from water to the membrane interface, following a Boltzmann distribution. These results suggest that following stimulation, the SNARE...
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PMID: 20937897
PDF is available here.
PDF is available here.
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Abstract
We investigated whether annexin-A2-induced lipid microdomains might be linked to the SNAREs present at the plasma membrane. Stimulation of adrenergic chromaffin cells induces the translocation of cytosolic annexin-A2 to the plasma membrane, where it colocalizes with SNAP-25 and S100A10. Cross-linkin...
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PMID: 20374557
PDF is available here.
PDF is available here.
Abstract
We investigated whether annexin-A2-induced lipid microdomains might be linked to the SNAREs present at the plasma membrane. Stimulation of adrenergic chromaffin cells induces the translocation of cytosolic annexin-A2 to the plasma membrane, where it colocalizes with SNAP-25 and S100A10. Cross-linkin...
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PMID: 20374557
PDF is available here.
PDF is available here.
Abstract
We investigated whether annexin-A2-induced lipid microdomains might be linked to the SNAREs present at the plasma membrane. Stimulation of adrenergic chromaffin cells induces the translocation of cytosolic annexin-A2 to the plasma membrane, where it colocalizes with SNAP-25 and S100A10. Cross-linkin...
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PMID: 20374557
PDF is available here.
PDF is available here.
Abstract
We investigated whether annexin-A2-induced lipid microdomains might be linked to the SNAREs present at the plasma membrane. Stimulation of adrenergic chromaffin cells induces the translocation of cytosolic annexin-A2 to the plasma membrane, where it colocalizes with SNAP-25 and S100A10. Cross-linkin...
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PMID: 20374557
PDF is available here.
PDF is available here.
Abstract
We investigated whether annexin-A2-induced lipid microdomains might be linked to the SNAREs present at the plasma membrane. Stimulation of adrenergic chromaffin cells induces the translocation of cytosolic annexin-A2 to the plasma membrane, where it colocalizes with SNAP-25 and S100A10. Cross-linkin...
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PMID: 20374557
PDF is available here.
PDF is available here.
Abstract
We investigated whether annexin-A2-induced lipid microdomains might be linked to the SNAREs present at the plasma membrane. Stimulation of adrenergic chromaffin cells induces the translocation of cytosolic annexin-A2 to the plasma membrane, where it colocalizes with SNAP-25 and S100A10. Cross-linkin...
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PMID: 20374557
PDF is available here.
PDF is available here.
Molecular Diversity (14)2 2010
Abstract
We describe a computational approach aimed at estimating the binding ability of epibatidine analogs to interact with the neuronal nicotinic acetylcholine receptor (nAChR) and get insights into the bioactive conformation. The protocol followed consists of a docking analysis and evaluation of pharmaco...
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PMID: 19479336
PDF is available here.
PDF is available here.
Biochemical Society transactions (38)Pt 1 2010
Abstract
We found that, in the absence of Munc18-1, the number of docked vesicles and the thickness of cortical F-actin are affected. In the present paper, I discuss the possible mechanism by which Munc18-1 modulates cortical F-actin and how it orchestrates the docking machinery via an interaction with synta...
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PMID: 20074058
PDF is available here.
PDF is available here.
Biochemical Society transactions (38)Pt 1 2010
Abstract
We summarize the current knowledge on the roles of myosin Va in the different steps of dense-core vesicle biogenesis and exocytosis, and compare findings obtained from different cell types and experimental systems....
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PMID: 20074059
PDF is available here.
PDF is available here.
Abstract
Myosin VI (MVI) is the only known myosin walking towards minus end of actin filaments. Here, MVI, but not myosins IB or IIB, was detected in chromaffin granules isolated from bovine medulla and found to be tightly associated with the granule apical surface. MVI also localized to secretory granules w...
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PMID: 20198214
PDF is available here.
PDF is available here.
Journal of Physiology (587)Pt 22 2009
Abstract
We have compared sites of exocytosis and the spatial distribution of calcium channels in chromaffin cells. Fusion of individual vesicles was visualized using interference reflection microscopy and the submembranous calcium signal was assessed using total internal reflection fluorescence microscopy....
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PMID: 19752110
PDF is available here.
PDF is available here.
Endocrinology (150)8 2009
Abstract
We sought to determine whether cysteine protease cathepsin L (CTSL), a novel enzyme for proteolytic processing of neuropeptides, acts like the well-established serine proteases [prohormone convertase (PC)1/3 or PC2] to generate catestatin by proteolytic processing of CgA. We found that endogenous CT...
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PMID: 19372204
PDF is available here.
PDF is available here.
Journal of Neurochemistry (109)3 2009
Abstract
We observed that IL-1beta increases the release of NPY, norepinephrine (NE), and epinephrine (EP) from human chromaffin cells. Moreover, the immunoneutralization of released NPY inhibits catecholamine release evoked by IL-1beta. Moreover, IL-1beta regulates catecholamine synthesis as the inhibition...
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PMID: 19309436
PDF is available here.
PDF is available here.
Abstract
We reviewed our recent work on the chronic effects of nicotine on the Na+ -Ca2+ exchanger (NCX) gene and protein expressions in various organs of rats treated with nicotine in the drinking water for 4-12 weeks. Microarray analysis and reverse transcriptase-polymerase chain reaction (RT-PCR) did not...
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PMID: 19252272
PDF is available here.
PDF is available here.
Abstract
We explore this approach by determining the solution structure and functional characteristics of a dicarba analogue of the alpha-conotoxin alpha-ImI, (2,8)-dicarba-(3,12)-cystino alpha-ImI. The structure of the dicarba analogue was similar to that of native alpha-ImI, with differences attributable t...
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PMID: 19125616
PDF is available here.
PDF is available here.
Journal of Neurochemistry (108)4 2009
Abstract
We investigate the specific regulation of the NO synthase (NOS) isoforms, inducible NOS (iNOS) and neuronal NOS (nNOS), and their particular participation in cell death induced by interferon gamma (IFNgamma). Lipopolysaccharide (LPS) and IFNgamma increase iNOS expression, with no effect on nNOS expr...
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PMID: 19141072
PDF is available here.
PDF is available here.
Frontiers in Bioscience (14)14 2009
Abstract
I will focus on these recent topics. Comprehensive review articles about lithium (1), GSK-3 and GSK-3 inhibitors (2-4), and the inhibition of Wnt/GSK-3beta>/beta-catenin signaling pathway by therapeutic drugs (5) are useful. Chemical structures of GSK-3 inhibitors are listed in the review articles (...
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PMID: 19273146
PDF is available here.
PDF is available here.
Abstract
We looked for a series of ultrastructural changes regarded as highly specific for PMD. We calculated the percentage of (i) resting, unaltered granules (type 1 granules); (ii) granules with changes indicative of progressive release of secretory materials, that is, granules with lucent areas in their...
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PMID: 18727112
PDF is available here.
PDF is available here.
Abstract
Glycogen synthase kinase-3 (GSK-3) is constitutively active in nonstimulated cells, where the majority of its substrates undergo inactivation/proteolysis by phosphorylation. Extracellular stimuli (e.g., insulin) catalyze inhibitory Ser(9)-phosphorylation of GSK-3beta, turning on signaling and causin...
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PMID: 19179806
PDF is available here.
PDF is available here.
Biochemistry (Washington) (47)52 2008
Abstract
We show that a Cav1.2-mutated α11.2/L775P subunit which does not conduct Ca2+ currents supports depolarization-evoked release by means of Ca2+ binding to the pore. Bovine chromaffin cells, which secrete catecholamine almost exclusively via nifedipine-sensitive Cav1.2, were infected with the Semliki...
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PMID: 19061337
PDF is available here.
PDF is available here.
Abstract
Chromaffin vesicles (CV) are highly sophisticated secretory organelles synthesized in adrenal medullary chromaffin cells. They contain a complex mixture of structural proteins, catecholamine neurotransmitters, peptide hormones, and the relative processing enzymes, as well as protease inhibitors. In...
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PMID: 19037853
PDF is available here.
PDF is available here.
Abstract
A number of promising cell therapy technologies have been identified. These provide targeted approaches to delivery of antinociceptive molecules, avoiding subjecting the patient to systemic toxicity of drugs. There has been considerable progress in treating degenerative joint diseases causing pain....
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PMID: 18990072
PDF is available here.
PDF is available here.
Journal of Neurochemistry (107)6 2008
Abstract
We used optical and electron microscopic probes to measure the effects of long-term METH exposure on secretory vesicle pH, and amperometry and intracellular patch electrochemistry to observe the effects on neurosecretion and cytosolic catecholamines in cultured rat chromaffin cells. In contrast to a...
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PMID: 19014382
PDF is available here.
PDF is available here.
European Journal of Pharmacology (598)1-3 2008
Abstract
We have explored how low concentrations of nicotine in the range of those found in the blood of tobacco smokers, might affect the release of catecholamines in bovine chromaffin cells. We have combined patch-clamp and Ca(2+) imaging techniques to study cell excitability, cytosolic Ca(2+) transients,...
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PMID: 18831972
PDF is available here.
PDF is available here.
Neuroscience Letters (445)1 2008
Abstract
We have cultured chromaffin cells from an alternative large animal model, the deer, and have conducted the first characterization of secretion from this preparation. Cervine chromaffin cells, preloaded with [3H]noradrenalin, displayed a strong secretory response to the cholinergic agonist carbachol,...
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PMID: 18775475
PDF is available here.
PDF is available here.
Neuroscience Letters (445)1 2008
Abstract
We investigated the cellular mechanisms underlying sensitivity to glucose-free solution (aglycemia) using ratiometric Ca2+ imaging and whole-cell recording. Though few cells (< 15%) responded to aglycemia with an increase in intracellular-free Ca2+ concentration ([Ca2+]i), in most cells (approximate...
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PMID: 18786611
PDF is available here.
PDF is available here.
Journal of Neurophysiology (100)5 2008
Abstract
We used carbon fiber amperometry to analyze catecholamine release from individual vesicles in bovine adrenal chromaffin cells, a widely used neurosecretory model. To bypass the effects of Gbetagamma on Ca(2+) entry, we stimulated secretion using ionomycin (a Ca(2+) ionophore) or direct intracellular...
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PMID: 18815342
PDF is available here.
PDF is available here.
Abstract
We propose that SNARE/lipid complexes form proteolipid fusion pores. Fusion pores involving the SNAP-25Delta9 mutant will be less tightly zipped and may lead to a longer fusion pore structure, consistent with the observed decrease of fusion pore conductance....
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PMID: 18829435
PDF is available here.
PDF is available here.
Biomedical Microdevices (10)5 2008
Abstract
We report the development of nitrogen doped diamond-like carbon (DLC:N) microelectrodes on a chip to monitor quantal release of catecholamines from cells. Advantages of DLC:N microelectrodes are that they are batch producible at low cost, and are harder and more durable than graphite films. The DLC:...
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PMID: 18493856
PDF is available here.
PDF is available here.
Endocrinology (149)10 2008
Abstract
We report that short-term treatment with glucocorticoids, released from adrenal cortex cells in response to chronic stress, inhibits activity-dependent potentiation (ADP) of catecholamine release. First, short-term treatment with dexamethasone (DEX), a synthetic glucocorticoid, reduces ADP in a conc...
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PMID: 18583423
PDF is available here.
PDF is available here.
Abstract
The aim of the present study was to verify if human FSH influences the adrenal gland of the newt, Triturus carnifex. Newts were given intraperitoneal injections of human FSH throughout the periods of February-March, and December-January; periods in which newt FSH levels are normally very low. The ef...
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PMID: 18606237
PDF is available here.
PDF is available here.
Abstract
We screened for dominant-negative mutants of yeast Sec1 by random mutagenesis of a GAL1-regulated SEC1 plasmid. Mutants were identified on the basis of galactose-inducible growth arrest and inhibition of invertase secretion. This effect of dominant-negative sec1 was suppressed by overexpression of t...
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PMID: 18757920
PDF is available here.
PDF is available here.
Abstract
We examined the mechanism for pore dilation utilizing a combined approach of peptide transfection, electrophysiology, electrochemistry and quantitative imaging techniques. We report that disruption of dynamin I function alters both fusion modes. Under low stimulation, interference with dynamin I doe...
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PMID: 18492483
PDF is available here.
PDF is available here.
Journal of Neuroscience (28)34 2008
Abstract
We have increased the distance between the coiled-coil SNARE motif and the transmembrane domain of the vesicular SNARE synaptobrevin-2 by insertion of a flexible linker to analyze how an increased intermembrane distance affects exocytosis. Synaptobrevin-2 lengthening did not change the frequency of...
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PMID: 18716205
PDF is available here.
PDF is available here.
Abstract
We have developed an algorithm to analyze secretory vesicle distribution and docking in electron micrographs. Our algorithm automatically detects the vesicles and calculates their distance to the plasma membrane on basis of the pixel coordinates, ensuring that all vesicles are counted and the shorte...
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PMID: 18577400
PDF is available here.
PDF is available here.
Journal of Physiology (586)16 2008
Abstract
We were surprised to find an increase in the occurrence of mEPCs with abnormally long half-widths at the neuromuscular junctions of Rab3A(-/-) mice. The abnormal miniature endplate currents (mEPCs), which have significantly greater charge than the average mEPCs for the same fibres, could arise from...
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PMID: 18591190
PDF is available here.
PDF is available here.
Journal of Physical Chemistry B (112)30 2008
Abstract
Exocytosis of a single bovine adrenal chromaffin cell, triggered by histamine stimulation, was investigated via the electric responses detected with single-walled carbon-nanotube field-effect transistors (SWCNT-FET) and the morphological changes acquired by atomic force microscopy (AFM). Secretion o...
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PMID: 18598074
PDF is available here.
PDF is available here.
Circulation (118)3 2008
Abstract
We established heritability of catestatin in twins from 2 continents. Linkage identified 3 regions contributing to catestatin, likely novel determinants of sympathochromaffin exocytosis. At 1 such positional candidate (ATP6N1), variation influenced CHGA secretion and processing to catestatin, confir...
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PMID: 18591442
PDF is available here.
PDF is available here.
Biochemistry (Washington) (47)27 2008
Abstract
We used a transcriptome/proteome-wide approach, to discover binding partners for CHGA, by employing a phage display cDNA library method. Several proteins within or adjacent to the secretory pathway were initially detected as binding partners of recombinant human CHGA. We then focused on the trans-Go...
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PMID: 18549247
PDF is available here.
PDF is available here.
Neuropharmacology (55)1 2008
Abstract
The ability of calcineurin to regulate IRS-1 and IRS-2 levels has not been examined in any given cells, although calcineurin inhibition by therapeutic immunosuppressants produced cytoprotective and cytotoxic effects (e.g., new-onset of diabetes mellitus, seizure). Chronic (>or=3h) treatment of cultur...
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PMID: 18538359
PDF is available here.
PDF is available here.
Journal of Neurochemistry (106)1 2008
Abstract
Neuropeptide Y (NPY) functions as a peptide neurotransmitter and as a neuroendocrine hormone. The active NPY peptide is generated in secretory vesicles by proteolytic processing of proNPY. Novel findings from this study show that cathepsin L participates as a key proteolytic enzyme for NPY productio...
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PMID: 18410501
PDF is available here.
PDF is available here.
Journal of Neuroscience (28)26 2008
Abstract
We reported, in addition to the central control through cholinergic innervation, a local gap junction-delineated route between adrenal chromaffin cells contributes to catecholamine exocytosis. Here, we investigated whether this intercellular communication is modified when the hormonal demand is incr...
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PMID: 18579734
PDF is available here.
PDF is available here.
Abstract
We show here that PLD1 is regulated by ribosomal S6 kinase 2 (RSK2)-dependent phosphorylation. RSK2 is activated by a high K(+)-induced rise in cytosolic calcium. Expression of inactive RSK2 mutants or selective knockdown of endogenous RSK2 dramatically affects the different kinetic components of th...
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PMID: 18550821
PDF is available here.
PDF is available here.
Neuroscience Letters (438)1 2008
Abstract
We recently established adrenal medullary cell line tsAM5D, which was immortalized by use of a temperature-sensitive mutant of the oncogene simian virus 40 large T-antigen. In the present study, when co-treated with glial cell line-derived neurotrophic factor (GDNF) and ciliary neurotrophic factor (...
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PMID: 18455310
PDF is available here.
PDF is available here.
Journal of Cell Biology (181)5 2008
Abstract
We discovered specific defects in neurosecretion and vesicular morphology in neuroendocrine cells and hippocampal synapses at the single vesicle level in sdy mice. These defects include larger vesicle size, slower quantal vesicle release, lower release probability, and smaller total population of th...
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PMID: 18504299
PDF is available here.
PDF is available here.
Bioelectrochemistry (73)1 2008
Abstract
Electrically excitable bovine adrenal chromaffin cells were exposed to nanosecond duration electric pulses at field intensities ranging from 2 MV/m to 8 MV/m and intracellular calcium levels ([Ca(2+)](i)) monitored in real time by fluorescence imaging of cells loaded with Calcium Green. A single 4 n...
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PMID: 18407807
PDF is available here.
PDF is available here.
Abstract
Abstract
We monitored HIF-2alpha expression and oxygen consumption in an O2-sensitive immortalized rat adrenomedullary chromaffin (MAH) cell line. Hypoxia (2-8% O2) caused a concentration- and time-dependent increase in HIF-2alpha induction, which was blocked in MAH cells with either RNA interference knockdo...
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PMID: 18353899
PDF is available here.
PDF is available here.
Abstract
We first examined the responsiveness of the cells to ligands of the glial cell line-derived neurotrophic factor (GDNF) family. tsAM5D cells proliferated at the permissive temperature of 33 degrees C in response to either GDNF or neurturin, but not persephin or artemin. At the nonpermissive temperatu...
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PMID: 18293415
PDF is available here.
PDF is available here.
European Journal of Pharmacology (586)1-3 2008
Abstract
These results suggest that glycogen synthase kinase-3beta inhibition caused down-regulation of Akt1 mRNA and Akt1 protein levels; conversely, constitutive activity of glycogen synthase kinase-3beta maintains steady-state level of Akt1 in quiescent adrenal chromaffin cells....
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PMID: 18395711
PDF is available here.
PDF is available here.
Abstract
We have previously reported that maternal 50% food restriction during late pregnancy and lactation reduces adrenal weight and impairs chromaffin cell differentiation in male rats at weaning. In addition, maternal undernutrition modifies the expression of several genes involved in proliferation and a...
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PMID: 18401834
PDF is available here.
PDF is available here.
Endocrinology (149)6 2008
Abstract
We have recently documented the action of the cytokine TNF-alpha on neuropeptide secretion and biosynthesis in isolated bovine chromaffin cells. Here, we demonstrate that the type 2 TNF-alpha receptor (TNF-R2) mediates TNF-alpha signaling in chromaffin cells via activation of nuclear factor (NF)-kap...
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PMID: 18292192
PDF is available here.
PDF is available here.
Journal of Neurochemistry (105)5 2008
Abstract
We found that both broad spectrum PKC antagonists and rottlerin, a selective inhibitor of the novel isoforms PKC theta and PKC delta, decreased quantal size and the number of secretory events recorded per stimulus. In contrast, drugs that selectively inhibit the atypical and conventional PKC isoform...
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PMID: 18248621
PDF is available here.
PDF is available here.
Abstract
This study presents a novel method for the direct, centrifugally induced fabrication of small, Ca2+-hardened alginate beads at polymer-tube micronozzles. The bead diameter can arbitrarily be adjusted between 180-800 microm by the nozzle geometry and spinning frequencies between 5-28 Hz. The size dis...
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PMID: 18465307
PDF is available here.
PDF is available here.
Journal of Neuroscience (28)21 2008
Abstract
We have examined catecholamine secretion from chromaffin cells in which both CAPS genes were deleted and show that the deletion of both CAPS isoforms causes a strong reduction in the pool of rapidly releasable chromaffin granules and of sustained release during ongoing stimulation. We conclude that...
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PMID: 18495893
PDF is available here.
PDF is available here.
Neuroscience Letters (436)2 2008
Abstract
We investigated whether orexin A would stimulate catecholamine release from rat and mouse adrenal chromffin cells. Puff application of orexin A dose-dependently induced amperometric currents in the cultured rat chromaffin cells, which was completely blocked by the selective OX1R antagonist SB-334867...
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PMID: 18395341
PDF is available here.
PDF is available here.
FASEB Journal (22)5 2008
Abstract
We investigated the effects of prenatal nicotine bitartrate exposure on the ability of neonatal (P0) rat AMCs to respond appropriately to asphyxial stressors. Control AMCs isolated from pups born to saline-treated dams displayed typical responses to hypoxia and hypercapnia, including inhibition of o...
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PMID: 18070822
PDF is available here.
PDF is available here.
Journal of Neuroscience (28)17 2008
Abstract
We apply single-cell electrophysiological, electrochemical, and fluorescence measurements to investigate the cellular mechanism for this shift in exocytic behavior. We show that at low-frequency stimulation, a filamentous-actin cell cortex plays a key role in stabilizing the kiss-and-run fusion even...
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PMID: 18434525
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)16 2008
Abstract
We have studied single vesicle exocytosis in chromaffin cells overexpressing an unphosphorylatable form (T18AS19A RLC-GFP) of myosin II that produces an inactive protein by patch amperometry. This method allows direct determination of fusion pore expansion by measuring its conductance, whereas the r...
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PMID: 18283106
PDF is available here.
PDF is available here.