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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Eukaryotic Cells (0):
Recent article
Eukaryotic Cells
Abstract
The Golgi is an ancient and fundamental eukaryotic organelle. Evolutionary cell biological studies have begun establishing the repertoire, processes, and level of complexity of membrane-trafficking machinery present in early eukaryotic cells. This article serves as a review of the li...
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PMID: 21646379
PDF is available here.
PDF is available here.
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Xenobiotica (41)5 2011
Abstract
We will describe the construction of a fusion protein with the SUMO-tag, its expression in Escherichia coli, and its purification followed by the removal of the SUMO-tag by a SUMO-specific protease in vitro....
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PMID: 19107426
PDF is available here.
PDF is available here.
Developmental Cell (20)1 2011
Abstract
As individual cells or groups of cells move through the complex environment of the body, their migration is affected by multiple external cues. Some cues are diffusible signaling molecules, and some are solid biophysical features. How do cells respond appropriately? This perspective...
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PMID: 21238921
PDF is available here.
PDF is available here.
Trends in Cell Biology (21)1 2011
Abstract
Trends in Cell Biology (21)1 2011
Abstract
Mutation Research (711)1-2 2011
Abstract
Genomic DNA is associated with various structural, regulatory, and transaction proteins. The dynamic and reversible association between proteins and DNA ensures the accurate expression and propagation of genetic information. However, various endogenous, environmental, and chemotherapeutic agents indu...
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PMID: 21185846
PDF is available here.
PDF is available here.
Cell Biology International (34)11 2010
Abstract
The 'microtrabecular lattice' (MTL) that Keith Porter described in the 1970s and 1980s is reconsidered as a proposed fundamental cytoplasmic structure of eukaryotic cells. Although considered to be an artefact by most cell biologists of his time (and probably ours), the case is made that something l...
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PMID: 20939832
PDF is available here.
PDF is available here.
Protein & Cell (1)11 2010
Abstract
We review studies on the dynamics of chromatin higher order structure and its relationship with gene transcription....
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PMID: 21153512
PDF is available here.
PDF is available here.
Cold Spring Harbor Protocols (2010)11 2010
Abstract
RNA interference (RNAi) is an evolutionarily conserved process that silences gene expression through double-stranded RNA species in a sequence-specific manner. Small interfering RNAs (siRNAs) can promote sequence-specific degradation and/or translational repression of target RNA by a...
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PMID: 21041394
PDF is available here.
PDF is available here.
Current protocols in protein science / editorial board, John E. Coligan ... [et al.] (Chapter 14) 2010
Abstract
I (GGTase I), and Rab geranylgeranyl transferase (also termed geranylgeranyltransferase type II - GGTase II). In this unit, synthetic isoprenoids conjugated to either a fluorophore or biotin group are used to assay the activity of protein prenyltransferases in vitro or to affinity tag prenylatable p...
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PMID: 21104984
PDF is available here.
PDF is available here.
Abstract
All complex life is composed of eukaryotic (nucleated) cells. The eukaryotic cell arose from prokaryotes just once in four billion years, and otherwise prokaryotes show no tendency to evolve greater complexity. Why not? Prokaryotic genome size is constrained by bioenergetics. The endosymbiosis that...
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PMID: 20962839
PDF is available here.
PDF is available here.
Abstract
The eukaryotic cell has evolved to compartmentalize its functions and transport various metabolites among cellular compartments. Therefore, in cell biology, the study of organization and structure/function relationships are of great importance. The endoplasmic reticulum is a highly convoluted, singl...
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PMID: 20810627
PDF is available here.
PDF is available here.
Abstract
A bicistronic expression vector can be used to generate stable cell lines expressing green fluorescent protein (GFP)-tubulin and photoactivatable (PA)-GFP-tubulin. This vector, pIRES (originally available from BD Biosciences/Clontech), expresses the gene of interest (GFP-tubulin) and a selection mar...
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PMID: 20810628
PDF is available here.
PDF is available here.
Abstract
The Drosophila larval neuromuscular junction (NMJ) shares many structural and functional similarities to synapses in other animals, including humans. These include the basic feature of synaptic transmission, as well as the molecular mechanisms regulating the synaptic vesicle cycle. Because of its la...
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PMID: 20810633
PDF is available here.
PDF is available here.
Abstract
Experimental evidence collected more than 20 years ago in different laboratories suggests that the interactions between live biological cells and micro- and nanoparticles depend on their metabolic state. These experiments were conducted by reputable groups, led by prominent leaders such as H. Pohl o...
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PMID: 20637326
PDF is available here.
PDF is available here.
Abstract
In bacteria, PriA protein, a conserved DEXH-type DNA helicase, plays a central role in replication restart at stalled replication forks. Its unique DNA-binding property allows it to recognize and stabilize stalled forks and the structures derived from them. Cells must cope with fork stalls caused by...
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PMID: 20658707
PDF is available here.
PDF is available here.
Abstract
Despite its universal importance for controlling gene expression, mRNA degradation was initially thought to occur by disparate mechanisms in eukaryotes and bacteria. This conclusion was based on differences in the structures used by these organisms to protect mRNA termini and in the RNases and modif...
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PMID: 20520623
PDF is available here.
PDF is available here.
Biochemistry (Washington) (49)23 2010
Abstract
The vacuolar (H(+))-ATPases are ATP-dependent proton pumps that acidify intracellular compartments and, in some cases, transport protons across the plasma membrane of eukaryotic cells. Intracellular V-ATPases play an important role in normal physiological processes such as receptor-mediated endocyto...
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PMID: 20450191
PDF is available here.
PDF is available here.
Abstract
We show via RNAi-mediated knockdown that ELYS, a nucleoporin critical for the recruitment of the essential Nup107/160 complex to chromatin, is required for NPC assembly at the end of mitosis but not during interphase. Conversely, the transmembrane nucleoporin POM121 is critical for the incorporation...
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PMID: 20550937
PDF is available here.
PDF is available here.
Abstract
Damage detection during nucleotide excision repair requires the action of multiple proteins that probe the DNA for different parameters like disruption of basepairing, DNA bendability and presence of chemical modifications. In a recent study it has been shown that two of these probing events can be...
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PMID: 20335079
PDF is available here.
PDF is available here.
Journal of Cell Science (123)Pt 9 2010
Abstract
We undertake an evolutionary analysis of 53 proteins known either for centriolar association or for involvement in cilia-associated pathologies. By linking protein distribution in 45 diverse eukaryotes with organism biology, we provide molecular evidence to show that basal-body function is ancestral...
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PMID: 20388734
PDF is available here.
PDF is available here.
Abstract
Our current understanding of the mechanism of translation is based on nearly fifty years of biochemical and biophysical studies. This mechanism, which requires the ribosome to manipulate tRNA and step repetitively along the mRNA, implies movement. High-resolution structures of the ribosome and its l...
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PMID: 20192783
PDF is available here.
PDF is available here.
Protoplasma (240)1-4 2010
Abstract
Phosphoinositides (PIs) are minor, but essential phospholipid constituents of eukaryotic membranes, and are involved in the regulation of various physiological processes. Recent genetic and cell biological advances indicate that PIs play important roles in the control of polar tip growth in plant ce...
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PMID: 20091065
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (26)4 2010
Abstract
The preparation of the gene-gun bullets, gene gun-mediated pEGFP, Pmcherry be able to separate and co-transfected into cultured MCF-7 cells, 24 h after transfection could be detected in red, green fluorescence, while the control group there was no fluorescent protein expression. CONCLUSION: Gene gun...
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PMID: 20368106
PDF is available here.
PDF is available here.
Abstract
In this study, a method was developed to measure replication rates of rolling-circle replicon-based plasmids in eukaryotic cells. This method is based on the discriminative quantitation of MboI-resistant, non-replicated input plasmids and DpnI-resistant, replicated plasmids. To do so, porcine circov...
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PMID: 20026194
PDF is available here.
PDF is available here.
Abstract
The expression of proteins which do not express well on their own can be enhanced by linking them to human serum albumin (HSA) or antibody crystallizable fragment (Fc). The constructs shown here are designed to secrete the proteins after transient transfection of mammalian cell lines. The fusion par...
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PMID: 20210749
PDF is available here.
PDF is available here.
médecine/sciences (26)3 2010
Abstract
Cellular RNAs are invariably organized in ribonucleoprotein particles, or RNPs, regardless of their size, structure or function. RNPs are monitored by active surveillance mechanisms for their structural and functional integrity at every single step of their "life". A limited number of key endoRNase...
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PMID: 20346275
PDF is available here.
PDF is available here.
Abstract
We here present the synthesis and application of a novel GDP-Capture Compound for the functional enrichment of GTPases, a pivotal protein family that exerts key functions in signal transduction. We present data from CCMS experiments on two biological lysates from Escherichia coli and from human-deri...
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PMID: 20026263
PDF is available here.
PDF is available here.
Abstract
Abstract
All organisms, from bacteria to humans, face the daunting task of replicating, packaging and segregating up to two metres (about 6 x 10(9) base pairs) of DNA when each cell divides. This task is carried out up to a trillion times during the development of a human from a single fertilized cell. The s...
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PMID: 20110988
PDF is available here.
PDF is available here.
Abstract
Mitochondria and plastids have their own DNAs and are regarded as descendants of endosymbiotic prokaryotes. Organellar DNAs are not naked in vivo but are associated with basic proteins to form DNA-protein complexes (called organelle nuclei). The concept of organelle nuclei provides a new approach to...
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PMID: 20467212
PDF is available here.
PDF is available here.
Abstract
We present a method for the preparation of crude ribosomal extracts from yeast cells, separation of the extract by sucrose gradient and interpretation of the results. This procedure is readily adaptable to mammalian cells....
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PMID: 20567211
PDF is available here.
PDF is available here.
Abstract
The single-stranded DNA binding proteins (SSBs) are required to maintain the integrity of the genome in all organisms. Replication protein A (RPA) is a nuclear SSB protein found in all eukaryotes and is required for multiple processes in DNA metabolism such as DNA replication, DNA repair, DNA recomb...
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PMID: 20012581
PDF is available here.
PDF is available here.
Abstract
Eukaryotic initiation of DNA replication is a tightly regulated process. In the yeasts, S-phase-specific cyclin Cdk1 complex as well as Dfb4-Cdc7 kinase phosphorylate the initiation factors Sld2 and Sld3. These factors form a ternary complex with another initiation factor Dbp11 in their phosphorylat...
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PMID: 20012578
PDF is available here.
PDF is available here.
Abstract
The discovery of microRNA (miRNA)-mediated gene silencing has added a new level of complexity to our understanding of post-transcriptional control of gene expression. Considering the ubiquity of miRNA-mediated repression throughout basic cellular processes, understanding its mechanism of action is p...
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PMID: 19841878
PDF is available here.
PDF is available here.
Abstract
Biochemistry (New York) (74)13 2009
Abstract
We systematize current data on the Ub-proteasome system, describe in detail proteasome structure, the ubiquitination system, and the classical ATP/Ub-dependent mechanism of protein degradation, as well as try to focus readers' attention on the existence of alternative mechanisms of proteasomal degra...
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PMID: 20210701
PDF is available here.
PDF is available here.
Abstract
Abstract
We here propose a methodology to develop plausible models for the driving mechanisms of embryonic and cancerous cell cycles. We first define a key property of the system (a cyclic behaviour in the case of the embryonic cell cycle) and set mathematical constraints on the types of two variable simplif...
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PMID: 19581341
PDF is available here.
PDF is available here.
Abstract
Abstract
We report that the inner kinetochore complex (CBF3) is required for pericentric DNA looping at the Cse4p-containing nucleosome. DNA within the pericentric loop occupies a spatially confined area that is radially displaced from the interpolar central spindle. Microtubule-binding kinetochore complexes...
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PMID: 19656849
PDF is available here.
PDF is available here.
Abstract
We summarize the evidence in support of the essential role that LDs play in longevity regulation and propose several molecular mechanisms by which these dynamic organellar compartments control the aging process in multicellular eukaryotes and yeast....
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PMID: 19754450
PDF is available here.
PDF is available here.
Bioscience Reports (29)5 2009
Abstract
We focus on the structural and mechanistic aspects of three RNA helicases, Dhh1, Upf1 and eIF4AIII, that are involved in eukaryotic mRNA decay....
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PMID: 19589129
PDF is available here.
PDF is available here.
Abstract
We survey experimental and computational approaches geared towards the identification of proximal and distal gene regulatory elements in the genomes of complex eukaryotes. Available approaches that decipher the genetic structure and function of regulatory elements by exploiting various sources of in...
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PMID: 19498043
PDF is available here.
PDF is available here.
Abstract
We know about Cif, from the bacterial gene to the host target....
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PMID: 19722840
PDF is available here.
PDF is available here.
Abstract
We investigated the effect of hydrodynamic stress on CHO cells in a fed-batch process using a previously reported system which subjects cells to repetitive, high levels of hydrodynamic stress, quantified by energy dissipation rate (EDR). The results indicated that cell growth and monoclonal antibody...
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PMID: 19405151
PDF is available here.
PDF is available here.
Mutation Research (685)1-2 2010
Abstract
We focus on major players in the bulk replication, DNA polymerases of the B-family. We review the organization of the replication fork in eukaryotes in a historical perspective, analyze contemporary models and propose a new integrative model of the fork....
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PMID: 19682465
PDF is available here.
PDF is available here.
Abstract
We have reanalysed the phylogenetic distribution and phylogeny of the RJL family, taking advantage of the recent expansion of sequence data available from diverse eukaryotes. We found that RJL orthologs are much more widely distributed than previously assumed. At least one representative encoding an...
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PMID: 19393304
PDF is available here.
PDF is available here.
Journal of Molecular Biology (390)5 2009
Abstract
We determined the X-ray structures of oxidized and NADH-reduced forms of naturally folded recombinant murine AIF. Our structures reveal that CTC with the pyridine nucleotide is stabilized by (i) pi-stacking interactions between coplanar nicotinamide, isoalloxazine, and Phe309 rings; (ii) rearrangeme...
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PMID: 19447115
PDF is available here.
PDF is available here.
Journal of Molecular Biology (390)5 2009
Abstract
We showed that duplexes corresponding to those in Saccharomyces cerevisiae are only observed in vitro after addition of one of two proteins: Imp3p or Imp4p. Here, we used fluorescence-based and other in vitro assays to determine whether these proteins possess RNA chaperone activities and to assess w...
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PMID: 19482034
PDF is available here.
PDF is available here.
Mathematical Biosciences (220)2 2009
Abstract
We propose and give a proof in principle that intracellular RNA aptamers might perform this function. The mathematical model considers the following: Suppose I denotes a drug (inhibitor) that must be distributed spatially throughout a cell, but that tends to remain outside the cell due the transport...
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PMID: 19540245
PDF is available here.
PDF is available here.
Abstract
The review summarizes current data about mechanisms of signal transduction with participation of cAMP (cyclic adenosine monophosphate) and elements of the complex cAMP–protein kinase A (PKA) signal pathway in unicellular eukaryotes. Conceptions of evolutionary origin of eukaryotic signal transducti...
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PMID: 19514907
PDF is available here.
PDF is available here.
A stochastic model for estimation of mutation rates in multiple-replication proliferation processes.
Abstract
We propose a stochastic model based on the branching process for estimation and comparison of the mutation rates in proliferation processes of cells or microbes. We assume in this model that cells or microbes (the elements of a population) are reproduced by generations and thus the model is more sui...
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PMID: 18846374
PDF is available here.
PDF is available here.
Abstract
We provide a nonexhaustive comparison of methods to detect cell death with apoptotic or nonapoptotic morphologies, their advantages and pitfalls. These guidelines are intended for investigators who study cell death, as well as for reviewers who need to constructively critique scientific reports that...
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PMID: 19373242
PDF is available here.
PDF is available here.
Biochemical Society transactions (37)Pt 4 2009
Abstract
HGT (horizontal gene transfer) is recognized as an important force in bacterial evolution. Now that many eukaryotic genomes have been sequenced, it has become possible to carry out studies of HGT in eukaryotes. The present review compares the different approaches that exist for identifying HGT genes...
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PMID: 19614596
PDF is available here.
PDF is available here.
Journal of Cell Science (122)Pt 16 2009
Abstract
We characterize the molecular and functional features of a novel protein called SPOC1. SPOC1 RNA expression was previously reported to be highest in highly proliferating tissues and increased in a subset of ovarian carcinoma patients, which statistically correlated with poor prognosis and residual d...
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PMID: 19638409
PDF is available here.
PDF is available here.
Mitochondrion (9)4 2009
Abstract
We discuss the idea that mitochondria provide the pool of PE necessary for the autophagosome biogenesis and we propose some possible experimental approaches aimed to explore this possibility....
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PMID: 19398041
PDF is available here.
PDF is available here.
Molecular Biotechnology (42)2 2009
Abstract
Mutation and selection are the essential steps of evolution. Researchers have long used in vitro mutagenesis, expression, and selection techniques in laboratory bacteria and yeast cultures to evolve proteins with new properties, termed directed evolution. Unfortunately, the nature of mammalian cells...
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PMID: 19367473
PDF is available here.
PDF is available here.
Abstract
We cannot say anything about their information content in the semantic sense. The semantic information content of genes is context-dependent. Genes namely assume their biochemical characteristics usually only within living cells, their developmental characteristics only within living organisms, and...
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PMID: 19712221
PDF is available here.
PDF is available here.
Abstract
Exosome complex is widely conserved, functionally versatile, and essential constituent of the machinery regulating gene expression in the nucleus as well as in the cytoplasm. While the most fundamental enzymatic property of exosome is ribonucleolytic activity, its in vivo functions a...
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PMID: 19467852
PDF is available here.
PDF is available here.