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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Germ Cells (2):
Recent article
Germ Cells
Abstract
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Abstract
Abstract
Our data highlights the important role of Eif5a and its hypusination for stem cell differentiation and proliferation....
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PMID: 21344951
PDF is available here.
PDF is available here.
Developmental Cell (20)3 2011
Abstract
We generated Mitopld mutant mice. The mice display meiotic arrest during spermatogenesis, demethylation and derepression of retrotransposons, and defects in primary piRNA biogenesis. Furthermore, in mutant germ cells, mitochondria and the components of the nuage, a perinuclear structure involved in...
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PMID: 21397847
PDF is available here.
PDF is available here.
PLoS Biology (9)3 2011
Abstract
We report on NHR-80/HNF4, a nuclear receptor that specifically mediates longevity induced by depletion of the germ line through a mechanism that implicates fatty acid monodesaturation.
nhr-80/HNF4 is induced in animals lacking a germ line and is specifically required for their extend...
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PMID: 21423649
PDF is available here.
PDF is available here.
Development (138)6 2011
Abstract
We show that, when grown at high temperature, a majority of synMuv B mutants irreversibly arrest at the L1 stage. High temperature arrest (HTA) is accompanied by upregulation of many genes characteristic of germ line, including genes encoding components of the synaptonemal complex and other meiosis...
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PMID: 21343362
PDF is available here.
PDF is available here.
Abstract
We have established a tethering system, in which the Gal4 DNA binding domain (DBD) of the Flag:Gal4 DBD:Ote▵LEM fusion protein physically interacts with the Gal4 binding sites upstream of bamP-gfp to artificially recruit the reporter gene gfp to the nuclear membrane. Our data demonstrated that the...
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PMID: 21356524
PDF is available here.
PDF is available here.
Abstract
We review the protocols for a yeast-based assay to detect effective ZFNs. Additionally, we detail the procedures for synthesis and injection of ZFN-encoding mRNA into zebrafish embryos, screening of injected embryos for induced mutations in the soma, and recovery of germline mutations....
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PMID: 21805278
PDF is available here.
PDF is available here.
Abstract
We studied the patterns of small RNAs targeting both strands of suffix in Drosophila ovaries using an RNase protection assay and the analysis of the corresponding RNA sequences from the libraries of total small RNAs. Our results indicate that suffix sense and antisense transcripts are targeted mainl...
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PMID: 21779345
PDF is available here.
PDF is available here.
Abstract
We previously showed that the TRAF family member TRAF2 mediates recruitment of HOIL-1L-interacting protein (HOIP) to the cytoplasmic domain of CD40, suggesting that HOIP has a role in the CD40 signaling pathway. To determine the role of HOIP in CD40 signaling, we used somatic cell gene targeting to...
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PMID: 21829693
PDF is available here.
PDF is available here.
Abstract
We show the derivation of iPSCs from human nonmobilized peripheral blood (PB) and bone marrow (BM) mononuclear cells (MNCs) by retroviral transduction of OCT3/4, SOX2, KLF4, and c-MYC. The PB- and BM-derived iPSCs were quite similar to human embryonic stem cells with regard to morphology, expression...
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PMID: 20497033
PDF is available here.
PDF is available here.
Abstract
Spermatogenesis consists of the mitotic division of spermatogonia, meiosis of spermatocytes, and postmeiotic differentiation of spermatids, processes tightly controlled by hormones and growth factors secreted by testicular somatic cells. The events during spermatogenesis are precisel...
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PMID: 21482414
PDF is available here.
PDF is available here.
Abstract
Epigenetic modifications of the genome are generally stable in somatic cells of multicellular organisms. In germ cells and early embryos, however, epigenetic reprogramming occurs on a genome-wide scale, which includes demethylation of DNA and remodeling of histones and their modifications. The mecha...
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PMID: 21030646
PDF is available here.
PDF is available here.
Abstract
We show that a novel chromatin factor, X non-disjunction factor 1 (xnd-1), is responsible for the global distribution of COs in C. elegans. xnd-1 is also required for formation of double-strand breaks (DSBs) on the X, but surprisingly XND-1 protein is autosomally enriched. We show that xnd-1 functio...
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PMID: 20944745
PDF is available here.
PDF is available here.
British Journal of Cancer (103)8 2010
Abstract
The majority of testicular germ cell cancers develop through a pre-invasive carcinoma in situ (CIS) stage. The CIS cell is a neoplastic counterpart of foetal germ cells. During their development, foetal germ cells undergo extensive and essential epigenetic modifications, but little is known about ep...
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PMID: 20823885
PDF is available here.
PDF is available here.
Theriogenology (74)4 2010
Abstract
We reported our preliminary studies on the isolation, characterization, and in vitro culture of sheep PGCs. Sheep PGCs can be identified with the markers previously used in mouse, but, in some cases, these markers are not coherently expressed in the same cell depending on the grade of differentiatio...
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PMID: 20580065
PDF is available here.
PDF is available here.
Scientific American (303)3 2010
Abstract
Abstract
The formation of both germline cysts and the germinal epithelium is described during the ovary development in Cyprinus carpio. As in the undifferentiated gonad of mammals, cords of PGCs become oogonia when they are surrounded by somatic cells. Ovarian differentiation is triggered whe...
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PMID: 20652938
PDF is available here.
PDF is available here.
Developmental Biology (344)2 2010
Abstract
We report a comprehensive analysis of gck-3 function and demonstrate its requirement for several developmental processes independent of ion homeostasis, i.e., larval progression, vulva, and germ line formation. Consistent with a wide range of gck-3 function we find that endogenous GCK-3 is expressed...
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PMID: 20595048
PDF is available here.
PDF is available here.
Abstract
Abstract
We have also introduced Penelope into Drosophila melanogaster, which are otherwise naive to the element. We have taken advantage of these natural and experimentally induced colonization processes to probe the evolution of small RNA pathways in response to transposon challenge. In both species, Penel...
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PMID: 20581131
PDF is available here.
PDF is available here.
Nature Cell Biology (12)8 2010
Abstract
We show here that the principal signalling pathway implicated in this niche, chemokine activation of STAT, does not primarily regulate self-renewal of germline stem cells (GSCs), but rather governs GSC adhesion to hub cells. In fact, GSC renewal does not require hub cell contact, as GSCs can be rene...
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PMID: 20622868
PDF is available here.
PDF is available here.
Nature Reviews: Genetics (11)8 2010
Abstract
The eukaryotic core promoter recognition complex was generally thought to play an essential but passive role in the regulation of gene expression. However, recent evidence now indicates that core promoter recognition complexes together with 'non-prototypical' subunits may have a vital regulatory fun...
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PMID: 20628347
PDF is available here.
PDF is available here.
Developmental Biology (344)1 2010
Abstract
We report the identification and characterisation of closca, a gene encoding a new member of a group of proteins that act non-redundantly in vitelline membrane biogenesis and in Torso signalling. We also show that the Nasrat protein, another member of this group, is incorporated into the vitelline m...
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PMID: 20457146
PDF is available here.
PDF is available here.
Genetics Research (92)4 2010
Abstract
TP5, a P element inserted in the telomere-associated sequences of the X chromosome, represses the excision of other P elements in the germ line through a combination of maternal and zygotic effects. The maternal component of this repression is impaired by heterozygous mutations in the aubergine and...
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PMID: 20943007
PDF is available here.
PDF is available here.
International Immunology (22)8 2010
Abstract
We have shown that STAT5 binds to the Jgamma promoters and controls chromatin accessibility by histone acetylation. However, little is known on control mechanism of Vgamma region by the IL-7R. To elucidate the regulation by STAT5, we first analyzed the chromatin status of Vgamma region in primary th...
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PMID: 20547543
PDF is available here.
PDF is available here.
Abstract
We discuss the biological and the biochemical properties of these proteins that indicate that they play a central and important role in eukaryotic cell biology....
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PMID: 20578993
PDF is available here.
PDF is available here.
Abstract
We identified the ciliate-specific Defective in IES Excision 5 (DIE5) genes of Paramecium tetraurelia (PtDIE5) and Tetrahymena thermophila (TtDIE5) as orthologs that encode nuclear proteins expressed exclusively during development. Abrogation of PtDie5 protein (PtDie5p) function by RNA interference...
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PMID: 20495055
PDF is available here.
PDF is available here.
Abstract
We show that DNA demethylation in the mouse PGCs is mechanistically linked to the appearance of single-stranded DNA (ssDNA) breaks and the activation of the base excision repair (BER) pathway, as is the case in the zygote where the paternal pronucleus undergoes active DNA demethylation shortly after...
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PMID: 20595612
PDF is available here.
PDF is available here.
Developmental Biology (343)1-2 2010
Abstract
We identify a possible PGC niche in the mouse genital ridges. Conditional ablation of Bmpr1a was used to demonstrate that BMP signaling is required for PGC survival and migration as these cells colonize the genital ridges. Reduced BMP signaling within the genital ridges led to increased somatic cell...
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PMID: 20417197
PDF is available here.
PDF is available here.
PLoS Genetics (6)7 2010
Abstract
We identified a Caenorhabditis elegans dual function DNA double-strand break repair and DNA damage signaling protein orthologous to the human GEN1 Holliday junction resolving enzyme. GEN-1 has biochemical activities related to the human enzyme and facilitates repair of DNA double-strand breaks, but...
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PMID: 20661466
PDF is available here.
PDF is available here.
Development (137)14 2010
Abstract
We demonstrate that Drosophila SmB and SmD3 are specific components of the oskar messenger ribonucleoprotein (mRNP), proper localization of which is required for establishing germline fate and embryonic patterning. Importantly, oskar mRNA is delocalized in females harboring a hypomorphic mutation in...
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PMID: 20570937
PDF is available here.
PDF is available here.
Development (137)14 2010
Abstract
We investigate whether EG cell self-renewal and derivation are supported by 2i. We report that mouse EG cells can be established with high efficiency using 2i in combination with the cytokine leukaemia inhibitory factor (LIF). Furthermore, addition of fibroblast growth factor or stem cell factor is...
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PMID: 20519324
PDF is available here.
PDF is available here.
Abstract
We show that Caenorhabditis elegans HIF-1 protects against DNA-damage-induced germ cell apoptosis by antagonizing the function of CEP-1, the homologue of the tumour suppressor p53. The antiapoptotic property of HIF-1 is mediated by means of transcriptional upregulation of the tyrosinase family membe...
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PMID: 20520707
PDF is available here.
PDF is available here.
Abstract
Animal Reproduction Science (119)1-2 2010
Abstract
We conclude that the Sertoli cell population stabilized in the pre-pubertal phase and germ cell population increased after puberty. The overall yield of spermatogenesis and Sertoli cell index were greater in animals around 12 months of age.
Copyright 2009 Elsevier B.V. All rights reserved....
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PMID: 19944546
PDF is available here.
PDF is available here.
Developmental Dynamics (239)5 2010
Abstract
We review the application of Caenorhabditis elegans as a model system to understand key aspects of stem cell biology. The only bona fide stem cells in C. elegans are those of the germline, which serves as a valuable paradigm for understanding how stem-cell niches influence maintenance and differenti...
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PMID: 20419785
PDF is available here.
PDF is available here.
Developmental Dynamics (239)5 2010
Abstract
We identify prp-17 as another gene functioning downstream of GLP-1 signaling that promotes meiotic entry, largely by acting on the GLD-1 pathway, and that also functions in female germline sex determination. PRP-17 is orthologous to the yeast and human pre-mRNA splicing factor PRP17/CDC40 and can re...
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PMID: 20419786
PDF is available here.
PDF is available here.
Developmental Dynamics (239)5 2010
Abstract
In most species, each sex produces gametes, usually either sperm or oocytes, from its germline during gametogenesis. The sperm and oocyte subsequently fuse together during fertilization to create the next generation. This review focuses on spermatogenesis and the roles of sperm during fertilization...
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PMID: 20419782
PDF is available here.
PDF is available here.
Developmental Biology (340)2 2010
Abstract
We identify a novel allele of bazooka (baz), the Drosophila homolog of Par-3, which has allowed the analysis of baz function throughout oogenesis. We demonstrate that baz is required for spatial restriction of the germ plasm and axis patterning, and we uncover multiple requirements for baz in regula...
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PMID: 20152826
PDF is available here.
PDF is available here.
Abstract
Distant hybridization makes it possible to transfer the genome of one species to another, which results in changes in phenotypes and genotypes of the progenies. This study shows that distant hybridization or the combination of this method with gynogenesis or androgenesis lead to different ploidy fis...
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PMID: 20596907
PDF is available here.
PDF is available here.
Abstract
We review the current status and future directions of understanding and manipulating fish germ cells in basic research and reproductive technology....
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PMID: 20596909
PDF is available here.
PDF is available here.
Abstract
Three germ cell layers, the ectoderm, mesoderm and endoderm, are established during the gastrulation stage. All cell types in different organs and tissues are derived from these 3 germ cell layers at later stages. For example, skin epithelial cells and neuronal cells are derived from the ectoderm, w...
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PMID: 20596911
PDF is available here.
PDF is available here.
Reproduction (139)4 2010
Abstract
We demonstrated the successful cryopreservation of PGCs by vitrification of yolk-removed embryos and the production of fertile zebrafish possessing germ cells that originated from the PGCs in vitrified embryos....
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PMID: 20154175
PDF is available here.
PDF is available here.
Pacific health dialog : a publication of the Pacific Basin Officers Training Program and the Fiji School of Medicine (16)1 2010
Abstract
Transgenic Research (19)2 2010
Abstract
We structure an ethical analysis of the transgenic marmoset case around three questions: whether it is acceptable to use animals as models of human disease; whether it is acceptable to genetically modify animals; and whether these animals' being monkeys makes a difference. The analysis considers the...
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PMID: 19690974
PDF is available here.
PDF is available here.
Transgenic Research (19)2 2010
Abstract
We structure an ethical analysis of the transgenic marmoset case around three questions: whether it is acceptable to use animals as models of human disease; whether it is acceptable to genetically modify animals; and whether these animals' being monkeys makes a difference. The analysis considers the...
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PMID: 19690974
PDF is available here.
PDF is available here.
Transgenic Research (19)2 2010
Abstract
We structure an ethical analysis of the transgenic marmoset case around three questions: whether it is acceptable to use animals as models of human disease; whether it is acceptable to genetically modify animals; and whether these animals' being monkeys makes a difference. The analysis considers the...
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PMID: 19690974
PDF is available here.
PDF is available here.
Transgenic Research (19)2 2010
Abstract
We structure an ethical analysis of the transgenic marmoset case around three questions: whether it is acceptable to use animals as models of human disease; whether it is acceptable to genetically modify animals; and whether these animals' being monkeys makes a difference. The analysis considers the...
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PMID: 19690974
PDF is available here.
PDF is available here.
Transgenic Research (19)2 2010
Abstract
We structure an ethical analysis of the transgenic marmoset case around three questions: whether it is acceptable to use animals as models of human disease; whether it is acceptable to genetically modify animals; and whether these animals' being monkeys makes a difference. The analysis considers the...
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PMID: 19690974
PDF is available here.
PDF is available here.
Transgenic Research (19)2 2010
Abstract
We structure an ethical analysis of the transgenic marmoset case around three questions: whether it is acceptable to use animals as models of human disease; whether it is acceptable to genetically modify animals; and whether these animals' being monkeys makes a difference. The analysis considers the...
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PMID: 19690974
PDF is available here.
PDF is available here.
Transgenic Research (19)2 2010
Abstract
We structure an ethical analysis of the transgenic marmoset case around three questions: whether it is acceptable to use animals as models of human disease; whether it is acceptable to genetically modify animals; and whether these animals' being monkeys makes a difference. The analysis considers the...
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PMID: 19690974
PDF is available here.
PDF is available here.
Abstract
Meiotic recombination, which promotes proper homologous chromosome segregation at the first meiotic division, normally occurs between allelic sequences on homologues. However, recombination can also take place between non-allelic DNA segments that share high sequence identity. Such non-allelic homol...
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PMID: 20164840
PDF is available here.
PDF is available here.
Abstract
We studied the reproductive biology of Mytella charruana to determine the potential reproductive success of this newly introduced bivalve species from Central/South America. We analyzed gonad morphology, gametogenesis, and the sex ratios of introduced populations throughout a 12 month period. In the...
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PMID: 19967719
PDF is available here.
PDF is available here.
Abstract
We term OSK) constitutes the minimal requirement for generating iPS cells from mouse embryonic fibroblasts. These cells are thought to resemble embryonic stem cells (ESCs) on the basis of global gene expression analyses; however, few studies have tested the ability and efficiency of iPS cells to con...
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PMID: 20139965
PDF is available here.
PDF is available here.
Current Biology (20)4 2010
Abstract
We show that kri-1, an ortholog of KRIT1/CCM1, which is mutated in the human neurovascular disease cerebral cavernous malformation, is required to activate DNA damage-dependent cell death independently of cep-1/p53. Interestingly, we find that kri-1 regulates cell death in a cell-nonautonomous manne...
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PMID: 20137949
PDF is available here.
PDF is available here.
Journal of Biotechnology (145)4 2010
Abstract
We examined its promoter fragment from +97 to -708 relative to the transcription start site and identified enhancer elements located between position -108 and +97. Experiments with transgenic flies revealed that the minimal promoter (from -108 to +20) is sufficient in the germline stem cells for the...
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PMID: 20026207
PDF is available here.
PDF is available here.
Molecular and Cellular Endocrinology (315)1-2 2010
Abstract
We provide an update on these latest findings for an emerging new concept regarding the presence of a local "apical ectoplasmic specialization-blood-testis barrier-hemidesmosome/basement membrane" functional axis that regulates the events of spermiation and blood-testis barrier (BTB) restructuring v...
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PMID: 19682538
PDF is available here.
PDF is available here.
Abstract
Although the pluripotent and proliferative capacity of embryonic germ (EG) cells is thought to be equivalent to that of embryonic stem (ES) cells, there has been far less attention focused on the potential use of EG cells for applications in developing novel strategies of tissue transplantation in t...
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PMID: 19548770
PDF is available here.
PDF is available here.
Abstract
We report production and characterization of monoclonal antibodies (MAb) that recognize PGCs. Extracts from E12.5 mouse embryonic gonads were immunized as an antigen, and hybridomas were generated using the rat medial iliac lymph node method. The hybridoma supernatants were screened by immunohistoch...
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PMID: 20199152
PDF is available here.
PDF is available here.