Ken-ichiro Hayashi,
Keisuke Horie,
Yuji Hiwatashi,
Hiroshi Kawaide,
Shinjiro Yamaguchi,
Atsushi Hanada,
Tamotsu Nakashima,
Masatoshi Nakajima,
Lewis N Mander,
Hisakazu Yamane,
Mitsuyasu Hasebe and
Hiroshi Nozaki
Abstract
We generated a PpCPS/KS disruption mutant that does not accumulate ent-kaurene. Phenotypic analysis demonstrates that the mutant has a defect in the protonemal differentiation of the chloronemata to caulonemata. Gas chromatography-mass spectrometry analysis shows that P. patens produces ent-kaurenoi...
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PMID: 20488896
PDF is available here.
Abstract
We describe the response of P. patens to UV-B radiation at the morphological and molecular levels. We find that P. patens is more capable of surviving UV-B stress than Arabidopsis (Arabidopsis thaliana) and describe the differential expression of approximately 400 moss genes in response to UV-B radi...
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PMID: 20427465
PDF is available here.
Abstract
The control activity of Rhodotorulaglutinis, salicylic acid (SA), alone or in combination, on gray mold spoilage and natural spoilage of strawberries was investigated. R. glutinis as stand-alone treatment, and the combined treatment of SA at 100 microg/mL with R. glutinis significantly reduced the d...
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PMID: 20488570
PDF is available here.
Abstract
These results indicate that HAT can effectively reduce fruit decay possibly by directly inhibiting pathogen growth and indirectly inducing disease resistance.
2010 Elsevier B.V. All rights reserved....
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PMID: 20510474
PDF is available here.
Abstract
We hypothesized that climatic variables that affect airborne mold counts affect the incidence of invasive aspergillosis (IA).
Patients who received hematopoietic stem cell transplants (HSCTs) in geographically and climatically diverse regions (Seattle, WA, and Houston, TX) were examined. Cumulative...
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PMID: 20450414
PDF is available here.
Abstract
The morphology and wall ultrastructure of megaspores and microspores of Isoetes sehnemii that grows in Brazil were analyzed as part of the study of the Isoetaceae present in Southern South America. The observations were performed with light, scanning and transmission electron microscopes. The megasp...
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PMID: 20563415
PDF is available here.
Abstract
The spore morphology and wall ultrastructure of Sphaeropteris gardneri (Hook.) R.M. Tryon from Brazil were analyzed with LM, SEM and TEM. The spores are trilete with an ornamentation formed of short low ridges with spines in their margins. The exospore is 2.5microm thick, two- layered in section and...
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PMID: 20563417
PDF is available here.
Abstract
Regnellidium diphyllum is considered as endangered, occurring in the State of Rio Grande do Sul, Brazil, and a few adjoining localities in Uruguay, Argentina and the State of Santa Catarina. It grows in wetlands frequently altered for agricultural activities. Herbicides based on 2,4-dichlorophenoxya...
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PMID: 20379656
PDF is available here.
Abstract
We sought to develop a nonlethal murine cutaneous model of IA. We induced cutaneous IA in cyclophosphamide-treated nude BALB/c mice by subcutaneous injection of Aspergillus fumigatus conidia. Skin lesion areas correlated well with tissue fungal burdens, allowing dynamic visual monitoring of cutaneou...
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PMID: 20145078
PDF is available here.
Abstract
We studied the morphogenesis of the gametophyte, and the morphology of the young sporophytes of four species: P. cretica, P. ensiformis, P. multifida and P. vittata, together with a palynological analysis that includes the ability of spores to germinate. Gametophytes were obtained trough in vitro cu...
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PMID: 20411709
PDF is available here.
Abstract
We focused our attention on CotC and CotU, two outer coat proteins known to be under CotE control and to form a heterodimer. We report here the results of pull-down experiments that provide the first direct evidence that CotE contacts other coat components. In addition, coexpression experiments demo...
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PMID: 20023017
PDF is available here.
Abstract
We recently reported on the strong interactions of zoospores of the green alga, Ulva linza with an arginine-rich oligopeptide self-assembled monolayer (SAM) [Biofouling 2008, 24, 303-312], where the arginine-rich peptide induced not only high spore settlement, but also a form of abnormal settlement,...
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PMID: 19719228
PDF is available here.
Abstract
We utilized the internal transcribed spacer (ITS) and beta-tubulin gene sequences to generate the phylogenetic relationship of T. formosanum and T. furfuraceum with other taxonomic relatives. Our analysis revealed five/three major phylogenetic clades according to the 5.8S-ITS2/beta-tubulin gene sequ...
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PMID: 19341392
PDF is available here.
Abstract
Bacillus atrophaeus' spores are used in the preparation of bioindicators to monitor the dry heat, ethylene oxide, and plasma sterilization processes and in tests to assess sterilizing products. Earlier production methods involved culture in chemically defined medium to support sporulation with the d...
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PMID: 19039586
PDF is available here.
Abstract
Freshly isolated oocysts were sporulated at 29 degrees C with constant aeration. Minimum sporulation time was determined by dosing the developing oocysts at intervals of 1 h to successive groups of chickens to assess the first occurrence of infective forms. Microscopic analysis of the sporulating oo...
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PMID: 6856329
PDF is available here.
Abstract
Adiponectin signal transduction pathway alteration presents in the myocardium of SHR, which might be one of the molecular mechanisms that cause hypertrophic metabolic abnormality. GD and GS could improve the hemodynamic index in SHR, and enhance the level of adiponectin and the expression of its rel...
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PMID: 19548441
PDF is available here.
Abstract
Light microscope observations of a haplosporidian in Diporeia spp. amphipods from Lakes Michigan and Huron, USA, found that the parasite spore is operculate and measures 8.1 microm in length and 6.1 microm in width. Round to amorphous sporocysts averaging 23.6 microm were present throughout hemal si...
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PMID: 19326796
PDF is available here.
Abstract
Two-spotted goby Gobiusculus flavescens from the Swedish Gullmarsfjord regularly present subcutaneous creamy-white patches in the body musculature, associated with Kabatana sp. infection. Analysis of the 16S rRNA gene of the microsporidium showed 98.54% homology with Kabatana newberryi infecting a m...
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PMID: 19326795
PDF is available here.
Abstract
This is the first report on pulmonary disease due to aspiration of Lycopodium spores in the course of fire-breathing. It shows that the anamnesis is as crucial as histology for the definitive diagnosis of aspiration disease....
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PMID: 18777466
PDF is available here.
BMC Microbiology (9 Suppl 1)suppl 1 2009
Abstract
We identify common features of the pathogenic processes of fungi and oomycetes and create a pathogenesis model using 256 newly developed and 38 extant GO terms, with an emphasis on the appressorium and signal transduction. This set of standardized GO terms provides a solid base to further compare an...
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PMID: 19278555
PDF is available here.
Abstract
The zoospores of the phytopathogenic Aphanomyces cochlioides are chemotactically attracted by a host-specific flavone, cochliophilin A (5-hydroxy-6,7-methylenedioxyflavone), and repelled from the mammalian estrogens or estrogenic compounds. This study further examined the responses of A. cochlioides...
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PMID: 20158156
PDF is available here.
Abstract
We review how chromatin structure and genome reprogramming regulate the sporulation transcription program, and how post-meiotic events reorganize spore chromatin....
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PMID: 19412896
PDF is available here.
Abstract
Fourteen blue-green and green algae survived for widely different time periods ranging between 22-102 d in control culture medium. Irrespective of their long or short survival period in control cultures, their pro- or eukaryotic nature, their different morphological types or natural habitats, they a...
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PMID: 19330547
PDF is available here.
Abstract
We analyzed the expression of several genes known to be involved in these pathways and selected one that was specifically expressed in sporangia and zoospores but not in mycelium. This gene, named PsGPA1, is a single-copy gene in P. sojae and encodes a G-protein alpha subunit that shares 96% identit...
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PMID: 18931042
PDF is available here.
Abstract
The morphogenesis of the sexual phase of seven species of Thelypteris subg. Cyclosorus (Thelypteridaceae) is described and compared. Spores of all species are monolete, ellipsoid and have positive photoblastism. They have Vittaria-type germination, germinal filaments are short and uniseriate, and As...
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PMID: 18358731
PDF is available here.
Abstract
The fern Blechnum sprucei grows in Mesoamerica (Costa Rica) and South America, from Colombia to Bolivia, SE and centre of Brazil, Paraguay and Argentina. It is a distinctive, somewhat vulnerable, mostly orophilous species. Fresh and dry herbarium material was used for this study. Herbarium material...
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PMID: 19419099
PDF is available here.
Abstract
Chlorine and iodine solutions were effective at inactivating Ichthyophonus spores in vitro. Inactivation in sea water increased directly with halogen concentration and exposure duration, with significant differences (P < 0.05) from controls occurring at all chlorine concentrations and exposure durat...
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PMID: 19238746
PDF is available here.
Ashok S AS Jadhav,
David C DC Taylor,
Michael M Giblin,
Alison M R AM Ferrie,
Stephen J SJ Ambrose,
Andrew R S AR Ross,
Ken M KM Nelson,
L L Irina Zaharia,
Nirmala N Sharma,
Maureen M Anderson,
Pierre R PR Fobert and
Suzanne R SR Abrams
Abstract
We have compared the effects of supplied ABA and the hydroxylated metabolites in regulating oil synthesis in microspore-derived embryos of B. napus, cv Hero that accumulate long chain fatty acids. Uptake into the embryos and metabolism of each of the hormone metabolites was studied by using deuteriu...
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PMID: 18823922
PDF is available here.
Abstract
Aflatoxin contamination resulting from maize infection by Aspergillus flavus is both an economic and a public health concern. Therefore, strategies for controlling aflatoxin contamination in maize are being investigated. The abilities of eleven naturally occurring atoxigenic isolates in Nigeria to r...
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PMID: 18608502
PDF is available here.
Abstract
Unisexuality, promoted by non-specific antheridiogens, enhances random mating both within and among species. The resulting hybridization can favour the reproductive exclusion of the allopolyploid in sites where it is outnumbered by diploids. However, the earlier production of gametangia in the allot...
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PMID: 18567915
PDF is available here.
Abstract
Pythium insidiosum causes pythiosis, a life-threatening disease that occurs in tropical regions and affects man and animals. Although pythiosis in Brazil had been described in various animal species, the first human case was only recently reported. The present study aimed to characterize the morphol...
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PMID: 19180750
PDF is available here.
Abstract
The use of copper surfaces within the clinical environment and application of a germination solution in infection control procedures may offer a novel way forward in eliminating C. difficile from contaminated surfaces and reducing CDAD....
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PMID: 18544601
PDF is available here.
Abstract
We established a procedure to prepare sporangia of M. polymorpha under laboratory conditions by promoting its transition to reproductive development by far-red light irradiation. Here we report an improved direct transformation system of M. polymorpha using immature thalli developing from spores. Hy...
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PMID: 18553085
PDF is available here.
Abstract
The 2001 anthrax letter cases brought into focus the need to establish the most effective environmental sampling procedures. Results are presented from two studies aimed at establishing the best procedures for everyday surfaces likely to be contaminated after the release of environmentally stable bi...
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PMID: 18637873
PDF is available here.
Abstract
We examine the current state of palynology in the field of forensic science. Forensic palynology is discussed with reference to other forensic disciplines to help understand what is required for its progress. Emerging developments are also discussed. Palynomorphs potentially deliver excellent trace...
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PMID: 18636981
PDF is available here.
Abstract
Out of 58 live tilapia fish, five Oreochromis niloticus were found to be naturally infected with Henneguya suprabranchiae (8.62%). Such infection was recorded only during winter season from Bahr Shebin, a tributary of the River Nile at Menoufia Governorate, Nile Delta, Egypt. Based on the structure...
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PMID: 18516619
PDF is available here.
Abstract
This study was carried out to augment the colonization of marine benthic communities on artificial reef structure. Increasing marine pollution along with various natural hazards cause severe damages to marine algae and associated fauna. In recent years, artificial reefs have been deployed in coastal...
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PMID: 19195405
PDF is available here.
Abstract
Attachment of spores of Ulva fasciata to natural biofilms was examined to probe the basis for specificity in settlement. Within 30 min from initial exposure in laboratory assays, spores attached to 1-, 3-, 6- or 9-day biofilms formed on acid-cleaned glass slides. The greatest number of spores attach...
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PMID: 19195406
PDF is available here.
Abstract
Algal spores respond to many environmental variables, especially to chemical "cues". This chemotactic response can be utilized to attract spores, thereby colonization of a new substrata is possible to be influenced. In this attempt, four chemoattractant candidates were screened against spores of Ulv...
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PMID: 19195407
PDF is available here.
Abstract
We investigated the structural distribution of both types of actin arrays, filaments and plaques, in a soil-borne phytopathogenic peronosporomycete (oomycete), Aphanomyces cochlioides, under standardized host-free bioassays. The phenomenon was monitored during progression through all the asexual dev...
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PMID: 18524561
PDF is available here.
Abstract
We used a number of gut isolates of Bacillus incorporating three species, B. subtilis, Bacillus licheniformis and Bacillus flexus to evaluate the nature of interaction between spores and the GALT. In mice orally administered with spores, evidence of cell proliferation was determined in the germinal...
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PMID: 18430003
PDF is available here.
Abstract
A newly discovered microsporidium infecting the black vine weevil, Otiorhynchus sulcatus (F.) (Coleoptera: Curculionidae), provisionally placed in the genus Canningia, was studied to determine its impact on O. sulcatus. O. sulcatus populations from several locations were sampled and evaluated for mi...
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PMID: 18539295
PDF is available here.
Abstract
We applied this methodology in a diastereo- and enantioselective iterative route and obtained two of the 16 possible stereoisomers of Phytophthora hormone alpha1. These synthetic stereoisomers induced the formation of sexual spores (oospores) in A2 mating type strains of three heterothallic Phytopht...
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PMID: 18559862
PDF is available here.
Abstract
We demonstrated the natural vulnerability of the ice sheet by using pollen records from marine sediment off southwest Greenland that indicate important changes of the vegetation in Greenland over the past million years. The vegetation that developed over southern Greenland during the last interglaci...
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PMID: 18566284
PDF is available here.
Abstract
This experiment had revealed two new bacteria species (P. polymyxa and J. lividum), associated with AMF spores, which functioned to suppress diseases and to solubilize P. SIGNIFICANCE AND IMPACT OF THE STUDY: AMF spores could be a useful source for bacterial antagonists to soil-borne diseases and P...
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PMID: 18217929
PDF is available here.
Abstract
A toxicity test using spore release of the aquatic green alga, Ulva, was developed and evaluated by assessing the toxicity of different organic and inorganic chemicals and elutriates of sewage or waste sludge. The toxic ranking of four metals was: Cu (EC50 of 0.040mgL(-1))>Cd (0.095mgL(-1))>Pb (0.48...
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PMID: 17954005
PDF is available here.
Abstract
These results indicate that myxospores of M. cerebralis demonstrate a selective rather than broad resistance to selected physical and chemical treatments, and this selective resistance is consistent with conditions that myxospores are likely to experience in nature....
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PMID: 18783133
PDF is available here.
Abstract
The asexual multinucleated sporangia of Phytophthora infestans can germinate directly through a germ tube or indirectly by releasing zoospores. The molecular mechanisms controlling sporangial cytokinesis or sporangial cleavage, and zoospore release are largely unknown. Sporangial cleavage is initiat...
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PMID: 18439859
PDF is available here.
Abstract
Myxobolus ampullicapsulatus n. sp. was isolated from the gills of Carassius auratus auratus (L., 1758) in Chongqing, China. Myxospores were pyriform, measuring 16.5-19.5 microm long x 8.5-10.0 microm wide x 7.0 microm thick. Two equal polar capsules were ampullaceous, measuring 7.0-10.0 microm long...
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PMID: 18605802
PDF is available here.
Abstract
We examined 4 laboratory populations of T. tubifex belonging to 16S mtDNA lineage III and 1 population belonging to 16S mtDNA lineage I for triactinomyxon (TAM) production after infection with M. cerebralis myxospores. All 4 16S mtDNA lineage III populations produced TAMs, but statistically signific...
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PMID: 18605778
PDF is available here.
Abstract
We examine the evolution of three candidate stress response networks (chemotaxis, competence for DNA uptake, and endospore formation) by analyzing their phylogenetic distribution across several hundred diverse bacterial and archaeal lineages. We report that genes in the chemotaxis and sporulation ne...
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PMID: 18495925
PDF is available here.
Abstract
We present a model and design methodology for the identification of nontoxic, antifouling surface topographies for use in the marine environment by the creation of engineered nanoforce gradients. The design and fabrication of these gradients incorporate discrete micrometer-sized features that are as...
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PMID: 18361532
PDF is available here.
Abstract
We have little knowledge of its allelpathic effects on ferns. In Petri dish bioassays, the inhibitory potential of aqueous leachates from roots, stems and leaves of A. adenophora was studied on the spore germination and gametophyte development of Macrothelypteris torresiana. All leachates inhibited...
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PMID: 18713423
PDF is available here.
Abstract
Our results showed that there are in total seven pathways of reproduction for E. prolifera including sexual, asexual and vegetative reproduction. It was indicated that the variety of the reproductive ways and the large quantity of reproductive cells produced and released during the reproductive seas...
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PMID: 18713431
PDF is available here.
Abstract
The interactive effects of an 8 h exposure to UV radiation and altered temperatures on the ultrastructure and germination of zoospores of the sublittoral brown alga Laminaria hyperborea (Gunn.) Foslie were investigated for the first time. Spores were exposed to four temperatures (2, 7, 12 and 17 deg...
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PMID: 18426486
PDF is available here.
Abstract
The effects of different concentrations (10(-5), 5x10(-5) and 10(-4)M) of copper bromide on spore germination, growth and ultrastructure were investigated in Polypodium cambricum L. gametophytes. The inhibitory effect of Cu was observed in spores cultured on medium supplemented with 10(-4)M CuBr(2):...
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PMID: 17904712
PDF is available here.
Abstract
To enable quantification of mycelial abundance in mixed-species environments, eight new TaqMan((R)) real-time PCR assays were developed for five arbuscular mycorrhizal fungal (AMF, Glomeromycota) taxa. The assays targeted genes encoding 18S rRNA or actin, and were tested on DNA from cloned gene frag...
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PMID: 17964831
PDF is available here.