I will first discuss some key notions, technical analogies, and images that are related to "fibre"-concepts from Andreas Vesalius to Albrecht von Haller and then focus on Charles Bonnet's and Denis Diderot's fibre ceconomies. In Bonnet's and Diderot's fibre economies, the self-active, regulating pro... | PMID: 20499615

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Surface engineering techniques for cellular micropatterning are emerging as important tools to clarify the effects of the microenvironment on cellular behavior, as cells usually integrate and respond the microscale environment, such as chemical and mechanical properties of the surrounding fluid and... | PMID: 20714311

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Peptides, polypeptides and proteins have been extensively studied for their various structural and functional roles in living organisms. However, breakthrough discoveries in the last decades identified some peptide-based matrices that posses the ability to traverse biological membranes, and many pep... | PMID: 20030616

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A significant number of therapeutic targets reside inside cells and intracellular organelles. Therapeutics therefore must be able to gain access to cellular compartments, and be able to interact specifically with a given molecule to exert a desired effect. Many naturally occurring toxins perform suc... | PMID: 20047149

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We have revealed that N(alpha)-myristoylation of several brain proteins unambiguously regulates certain protein-protein interactions that may affect signaling pathways in brain. Comparison of the amino acid sequences of myristoylated proteins including those in other organs suggests that this regula... | PMID: 20467215

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PMID: 20134137

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It remains controversial whether blastomeres of 2-cell stage mouse embryos show bias in their contribution to the blastocyst and whether there is any effect of superovulation. Two-cell stage embryos from CF1 mice were derived by either natural breeding (N) or superovulation (S) and cultured in vitro... | PMID: 20209440

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This article reviews one of the most important post-translational protein modifiers--ubiquitin. The basic mechanisms of conjugation reactions of ubiquitin with substrate, factors influencing the ubiquitination and its role in regulation of different cellular processes have been considered. | PMID: 21805857

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We discuss our recent investigations into the effect of mean pore size in collagen-glycosaminoglycan (CG) scaffolds with pore sizes ranging from 85–325 μm and how it has provided an insight into the divergence within the literature.... | PMID: 20421733

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We monitored autophagosome formation in living nonphagocytic cells by inducing autophagy around artificial micrometer-sized beads instead of bacteria. Beads conjugated with bio-reactive molecules provide a powerful tool for examining biochemical properties in vitro. However, this technique has not b... | PMID: 19901555

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The apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G or APOBEC3G) and its fellow cytidine deaminase family members are potent restrictive factors for human immunodeficiency virus type 1 (HIV-1) and many other retroviruses. However, the cellular function of APOBEC3G remain... | PMID: 19841882

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We have tagged tubulin with a photoactivatable variant of green fluorescent protein (PA-GFP), thereby allowing one to follow the behavior of a subset of tagged molecules in the cell. Here, we describe methods to tag and express proteins with PA-GFP, locally photoactivate the recombinant protein and... | PMID: 20719266

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We also provide a discussion with pitfalls and alternative techniques adapted to various substrates, including silicone elastomers. 2010 Elsevier Inc. All rights reserved.... | PMID: 20719269

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We describe a biochamber that allows for plant cell growth and development while allowing gas exchange and reducing evaporation. Another major difficulty is the limited fluorescence lifetime and we describe imaging strategies to reduce photobleaching in long-term imaging. We also discuss methods of... | PMID: 20719281

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I describe the materials and methods for cryo electron tomography of microtubules and in whole cells, describing cell growth, specimen vitrification, localization of microtubules, cryo electron tomography recording, tomographic image reconstruction, and 3D visualization techniques. 2010 Elsevier Inc... | PMID: 20719285

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We describe a method for automatically finding the location and conformations of microtubules in tomograms of high-pressure frozen, freeze substituted cells. Our approach uses two steps: a preprocessing step that finds locations in the tomograms that are likely to lie inside microtubules and a track... | PMID: 20719286

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We present approaches to assess MT dynamics in cultured mammalian cells using fluorescence redistribution after photobleaching. We include a discussion of cell culture and imaging conditions that maintain cell viability. We also provide an extensive discussion of both data collection and analysis th... | PMID: 20719264

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We describe the application of a commercially available setup to analyze microtubule behavior in live mammalian cells using TIRF microscopy. 2010 Elsevier Inc. All rights reserved.... | PMID: 20719267

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We use advanced methods for determining a set of optimized features for training an ensemble using random subspace with a set of Levenberg-Marquardt neural networks. The process requires that we first run several experiments to determine the individual features that offer the most information. The b... | PMID: 20865503

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We integrate this energetic concept inside the CPM. We simulate a cell differentiation inside a growing cell tissue. The results are the emergence of dynamic patterns coming from the consumption and production of energy. A model described by CA is less scalable than one described by a multi-agent sy... | PMID: 20865555

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We describe protocols for this procedure and describe its application to a number of vertebrate, fungal, protozoal, and plant sources.... | PMID: 20466127

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We present protocols that we have used for the analysis of tubulin isotypes and PTMs present in tubulin isolated from cells in culture or tissues and for the identification of tubulin regions altered by microtubule-stabilizing agents. Tubulin proteomics complements structural and computer modeling i... | PMID: 20466132

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We consider three distinct stages of postacquisition analysis: (1) processing of ECD mass spectra prior to the database search; (2) the database search step itself and (3) postsearch processing of results. We demonstrate that each of these steps has an effect on the number of peptides identified, wi... | PMID: 19821632

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We proposed a theoretical framework integrating the concepts of developmental biology with those of process design to provide the rules for the design of biomimetic processes. We named this methodology "developmental engineering" to emphasize that it is not the tissue but the process of in vitro tis... | PMID: 19589040

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