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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Fluorescent Dyes (0):
Recent article
Fluorescent Dyes
Abstract
We report on the use of 3,4,9,10-perylenetetracarboxylic diimide microfibers (PDIMs) as an effective fluorescent sensing platform for DNA detection for the first time. This sensing system exhibits a detection limit as low as 15 nmol L(-1) and has a high selectivity down to single-base mismatch. The...
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PMID: 21819867
PDF is available here.
PDF is available here.
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Acta Biomaterialia (7)8 2011
Abstract
A low density and high strength alloy, Ca65Mg15Zn20 bulk metallic glass (CaMgZn BMG), was evaluated by both in vitro tests on ion release and cytotoxicity and in vivo implantation, aimed at exploring the feasibility of this new biodegradable metallic material for potential skeletal a...
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PMID: 21571105
PDF is available here.
PDF is available here.
Organic Letters (13)14 2011
Abstract
A fluorescent sensor for cadmium (CS) based on the BODIPY fluorophore exploiting the PET (Photoinduced Electron Transfer) mechanism was prepared. CS exhibited high selectivity and sensitivity for detecting cadmium in aqueous buffer solution. In addition, the complex of CS with cadmiu...
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PMID: 21688791
PDF is available here.
PDF is available here.
Abstract
Fibrillar aggregates of beta-amyloid peptide (Abeta) are major constituents of the senile plaques found in the brains of patients suffering from Alzheimer's disease (AD). Previous studies have shown that spontaneous isomerization or racemization of aspartyl residues in Abeta peptides...
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PMID: 21812329
PDF is available here.
PDF is available here.
Abstract
Untargeted metabolomics on the plasma and urine from wild-type and organic anion transporter-1 (Oat1/Slc22a6) knockout mice identified a number of physiologically important metabolites, including several not previously linked to Oat1-mediated transport. Several, such as indoxyl sulfa...
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PMID: 21476605
PDF is available here.
PDF is available here.
Abstract
We attached the temperature sensitive dye-Rhodamine B (RhB), whose emission intensity decreases with increasing temperature-within the matrix of Pdots. The as-prepared Pdot-RhB nanoparticle showed excellent temperature sensitivity and high brightness because it took advantage of the light harvesting...
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PMID: 21548583
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
Trace fluorescent labeling, typically less than 1%, can be a powerful aid in macromolecule crystallization. Precipitation concentrates a solute, and crystals are the most densely packed solid form. The more densely packed the fluorescing material, the brighter the emission from it; thus, fluorescenc...
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PMID: 18542877
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
Majority of protein drugs in development today are glycoproteins e.g. recombinant antibodies expressed in various cell lines. Oligosaccharides through conformational changes can modulate therapeutic value (potency) of glycoproteins e.g. complement dependent cell cytotoxicity (CDCC) and antibody-depe...
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PMID: 18373267
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
Key enzymes that assemble the bacterial cell wall are also the target of the Beta-lactam class of antibiotics. The covalent binding of labeled penicillin to these proteins has been used in numerous studies in drug discovery, antibiotic mechanisms of action and resistance, and cell wall physiology. M...
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PMID: 18437311
PDF is available here.
PDF is available here.
Cold Spring Harbor Protocols (2011)5 2011
Abstract
Protein-DNA interactions (PDIs) are critical for many cellular processes. We present here a protocol for the identification of PDIs in vitro using protein microarray technology. The procedure involves double-stranding synthesized DNA oligonucleotides with a fluorescent-labeled primer, binding the la...
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PMID: 21536762
PDF is available here.
PDF is available here.
Abstract
The main objective of present research study was to evaluate the potential of lipid nanoparticles for active delivery of an antiretroviral drug to lymphatic tissues. Stavudine entrapped drug loaded solid lipid nanoparticles (SLNs) were prepared and characterized for a variety of phys...
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PMID: 21612153
PDF is available here.
PDF is available here.
Abstract
Molecular mobility in amorphous solid biomaterials is modulated by the composition and environment (primarily temperature). Phosphorescence of the triplet probe erythrosin B was used to generate a mobility map within amorphous sucrose films doped with starch ranging from 0.001 to 0.1 g starch/g sucro...
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PMID: 21381746
PDF is available here.
PDF is available here.
Abstract
The objective of this work was to study Fe distribution, and oxidative and nitrosative metabolism in Laternula elliptica for physiological analysis and interspecific comparisons. Lipid peroxidation, superoxide dismutase and catalase activity and total Fe content were estimated in the...
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PMID: 21094695
PDF is available here.
PDF is available here.
Abstract
We propose a microsphere array device with microspheres having controllable positions for error-free target identification. We conduct a statistical design analysis to select the optimal distance between the microspheres as well as the optimal temperature. Our design simplifies the imaging and ensur...
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PMID: 21342857
PDF is available here.
PDF is available here.
Abstract
We track division of 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled HSC in vivo. We found that, in steady-state mice, bone marrow cells capable of reconstituting lifelong hematopoiesis are found within both fast-cycling (undergoing five or more divisions in 7 wk) and quiesc...
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PMID: 21300914
PDF is available here.
PDF is available here.
Biomedical Materials (6)1 2011
Abstract
Engineering dermal substitutes with electrospun nanofibres have lately been of prime importance for skin tissue regeneration. Simple electrospinning technology served to produce nanofibrous scaffolds morphologically and structurally similar to the extracellular matrix of native tissu...
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PMID: 21205999
PDF is available here.
PDF is available here.
Biopolymers (95)5 2011
Abstract
Journal of Molecular Biology (405)3 2011
Abstract
We report a fluorescence-based assay for measuring the real-time kinetics of single-nucleotide incorporation during transcription elongation. The fluorescent adenine analogue 2-aminopurine was incorporated at various single positions in the template or the nontemplate strand of the promoter-free elo...
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PMID: 21035457
PDF is available here.
PDF is available here.
Biopolymers (95)5 2011
Abstract
We examine recent advances in the super-resolution imaging of living cells by reviewing recent breakthroughs in single molecule localization microscopy methods such as PALM and STORM to achieve this important goal. © 2011 Wiley Periodicals, Inc. Biopolymers 95: 322-331, 2011....
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PMID: 21254001
PDF is available here.
PDF is available here.
Abstract
We demonstrate that the enzyme β-lactamase, a naturally occurring enzyme expressed by M. tuberculosis, can be used for REF. The resulting imaging system is the first that allows non-invasive detection of natural M. tuberculosis strains directly in pulmonary infected living animals. Use of REF for M...
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PMID: 21178505
PDF is available here.
PDF is available here.
Thrombosis and Haemostasis (105)5 2011
Abstract
Macrophages contribute pivotally to cardiovascular diseases (CVD), notably to atherosclerosis. Imaging of macrophages in vivo could furnish new tools to advance evaluation of disease and therapies. Proteolytic enzymes serve as key effectors of many macrophage contributions to CVD. Therefore, intravit...
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PMID: 21225096
PDF is available here.
PDF is available here.
Tsitologiia (53)5 2011
Abstract
Fluorescent tags are extensively used for the diagnostic, labeling and marking in vivo and in vitro systems. In this study, fluorescent nanoparticles with Er/Yb lightning center were tested on neutrophilic granulocytes. The main purpose was to idenfity possible toxic effect. The negative impact of fl...
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PMID: 21786688
PDF is available here.
PDF is available here.
Abstract
We describe a biosensor based on an engineered fibronectin monobody scaffold that can be tailored to bind different targets via high-throughput screening. We made this Src-family kinase (SFK) biosensor by derivatizing a monobody specific for activated SFKs with a bright dye whose fluorescence increa...
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PMID: 21666688
PDF is available here.
PDF is available here.
Mikrobiyoloji bulteni (45)1 2011
Abstract
The majority of catheter-related bloodstream infections (CR-BSI) are associated with central venous catheters (CVCs) and most of them develop in patients staying at intensive care units (ICUs). The aim of this study was to assess the performance of different methods for the diagnosis of CR-BSI in neu...
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PMID: 21341162
PDF is available here.
PDF is available here.
Abstract
We found that for responses measured above 0.2%, inter-laboratory %CVs were, on average, 35%. No differences in inter-laboratory variation were observed if a 4-color antibody cocktail or a 7-color combination was used. Moreover, the data allowed identification of important sources of variability for...
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PMID: 20727897
PDF is available here.
PDF is available here.
Abstract
We present experimental methods based on selective labeling techniques and the use of effectors for a qualitative and quantitative evaluation of endocytic phenomena involving nanoparticles. The understanding of the cell-material interactions arising from these tests can then form the basis for a mod...
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PMID: 21042977
PDF is available here.
PDF is available here.
Abstract
We describe a method for imaging the position of nanoparticles within a 3D tissue-engineered model using confocal laser scanning microscopy (CLSM). The ability to track diffusion of nanoparticles in vitro is an important part of trans-dermal and trans-mucosal drug delivery development as well as for...
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PMID: 21042964
PDF is available here.
PDF is available here.
Abstract
We describe an improved fluorescent imaging approach to detect potential islet dysfunction. Using Cell Tracker Red (CTR, a mildly thiol-reactive fluorescent probe) to positively label particular islets, we measured intracellular free calcium with fura-2 AM in both CTR-labeled and unlabeled sets of p...
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PMID: 21266850
PDF is available here.
PDF is available here.
Cold Spring Harbor Protocols (2011)1 2011
Abstract
We demonstrate isolation, ex vivo growth, and fluorescent immunostaining of mouse embryonic day 12.5 (E12.5) kidneys. To demonstrate nephron formation using live imaging, we have isolated and cultured kidneys from mice carrying a green fluorescent protein (GFP) transgene driven by the Hes 1 promoter...
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PMID: 21205858
PDF is available here.
PDF is available here.
Abstract
The aim of the present study was to investigate microglia activation over time following traumatic brain injury (TBI) and to relate these findings to glutamate release.
Sequential dynamic (R)-[(11)C]PK11195 PET scans were performed in rats 24 hours before (baseline),...
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PMID: 21672189
PDF is available here.
PDF is available here.
Biopolymers (95)5 2011
Abstract
With the expansion of super-resolution fluorescence microscopy methods, it is now possible to access the organization of cells and materials at the nanoscale by optical means. This review discusses recent progress in super-resolution imaging of isolated and cell DNA using single-molecule localization...
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PMID: 21184489
PDF is available here.
PDF is available here.
Abstract
We have investigated the kinetics of binding, via this increase in DNA length, for single, hydrodynamically-stretched molecules of lambda DNA observed via Total Internal Reflection Fluorescence (TIRF) microscopy. The rate and degree of lengthening in 40mM NaHCO(3) (pH 8.0) buffer depend upon the fre...
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PMID: 21073861
PDF is available here.
PDF is available here.
Lab On a Chip (10)23 2010
Abstract
Oxygen tension is critical in a number of cell pathways but is often overlooked in cell culture. One reason for this is the difficulty in modulating and assessing oxygen tensions without disturbing the culture conditions. Toward this end, a simple method to generate oxygen-sensitive microwells was d...
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PMID: 20938500
PDF is available here.
PDF is available here.
Lab On a Chip (10)23 2010
Abstract
We propose a versatile multiplexed cell-counting method using functional silica nanoparticles (SiNPs). FITC-doped SiNPs, which are 100 times brighter than the FITC molecules itself, were used as new intensity-based fluorescent dye complexes to simultaneously measure two subsets of leukocytes using a...
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PMID: 20941407
PDF is available here.
PDF is available here.
Lab On a Chip (10)23 2010
Abstract
We report a low cost, disposable polymer microfluidic sample preparation device to perform rapid concentration of bacteria from liquid samples using enhanced evaporation targeted at downstream detection using surface enhanced Raman spectroscopy (SERS). The device is composed of a poly(dimethylsiloxa...
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PMID: 20938505
PDF is available here.
PDF is available here.
Lab On a Chip (10)23 2010
Abstract
Functional high-density micro-arrays for mass spectrometry enable rapid picolitre-volume aliquoting and ultrasensitive analysis of microscale samples, for example, single cells.
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PMID: 20938499
PDF is available here.
PDF is available here.
Abstract
A new probe for Cu(2+) based on the Cu(2+)- induced reversible ring-opening mechanism of the rhodamine spirolactam was described. It displayed a highly selective and sensitive "turn-on" fluorescent and colorimetric response toward Cu(2+).
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PMID: 20927482
PDF is available here.
PDF is available here.
Abstract
Two fluorescent heteroditopic ligands (2a and 2b) for zinc ion were synthesized and studied. The efficiencies of two photophysical processes, intramolecular charge transfer (ICT) and photoinduced electron transfer (PET), determine the magnitudes of emission bathochromic shift and enhancement, respec...
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PMID: 20882250
PDF is available here.
PDF is available here.
Abstract
An unexpected discovery of new trans-4-acetyl-1,9-dimethyl-4,4a-dihydro-3H-fluoren-3-ones from one pot reactions of benzaldehydes and acetylacetone is described. The synthetic mechanism and stereochemistry were discussed. These new derivatives exhibit good fluorescent properties in solutions.
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PMID: 20820667
PDF is available here.
PDF is available here.
Abstract
We have used ageladine A to stain acidic vesicles in cells and found the compound especially useful for tracking transport vesicles in cultured nerve cells. Inward as well as outward ionic currents appear not to be influenced by ageladine A at concentrations of 10 μM or less. Higher concentrations...
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PMID: 20955687
PDF is available here.
PDF is available here.
Abstract
The new macrocyclic ligand 1,9(4,7)-diphenanthroline-3,7,11,15-tetraazacyclohexadecaphane (L) was synthesized by a 2 : 2 reaction of 1,10-phenanthroline-4,7-dialdehyde with 1,3-diaminopropane, followed by reduction with NaBH(4). L contains two phenanthroline groups linked together by two 1,3-dia...
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PMID: 20922246
PDF is available here.
PDF is available here.
Chemical Communications (46)42 2010
Abstract
We report a new chemical biodelivery method for labeling hMSCs using a series of very efficient and highly versatile thiophene-based fluorescent dyes. Such conjugates contribute to a stable and viable labeling of hMSCs and constitute a promising strategy for in vivo applications....
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PMID: 20862424
PDF is available here.
PDF is available here.
Chemical Communications (46)42 2010
Abstract
The first ratiometric fluorescent probe for hydroxyl radical ratiometric imaging in living cells was rationally designed based on a hybrid coumarin-cyanine platform.
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PMID: 20856981
PDF is available here.
PDF is available here.
Abstract
A joint theoretical-experimental investigation has been carried out to unravel the details of the complexation of cations by fluoroionophores based on coumarin 343 and to interpret the modifications in the ligand and also in the coumarin structural, electronic, magnetic, and vibrational properties....
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PMID: 20882231
PDF is available here.
PDF is available here.
Brain research (1360) 2010
Abstract
Astaxanthin (AST) is a powerful antioxidant that occurs naturally in a wide variety of living organisms. Much experimental evidence has proved that AST has the function of eliminating oxygen free radicals and can protect organisms from oxidative damage. The present study was carried out to further i...
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PMID: 20846510
PDF is available here.
PDF is available here.
Brain research (1360) 2010
Abstract
The inability of axons in central nervous system (CNS) to regenerate after injury is related partly to multiple endogenous axon growth inhibitors including Nogo receptor (NgR). This study tested the hypothesis that silencing NgR expression by adenovirus-mediated RNA interference (RNAi) (AD-NgR) may...
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PMID: 20828545
PDF is available here.
PDF is available here.
Abstract
These results are in agreement with flow cytometry results. ITMC is a valuable tool to monitor the biological responses to nano-sized dosage forms such as NPs. Since the thermal activity of macrophage-NP interactions differed according to the type of NPs used, ITMC may provide a method to better und...
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PMID: 21057907
PDF is available here.
PDF is available here.
Abstract
Employing the utility of the native chemical ligation, site-specific attachment of an ultrastable perylene dye to a derivative of the major light-harvesting complex (LHCII) was demonstrated. Biochemical analysis of the conjugate indicated that the structure and function of LHCII remain largely unaff...
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PMID: 20820669
PDF is available here.
PDF is available here.
Abstract
A rhodamine-based sensor RB-S2 was designed and synthesized by combination of the thiospirolacton chromophore and the thiophen ring block with high affinity to Hg(2+). Probe RB-S2 exhibits high selectivity and excellent sensitivity in both absorbance and fluorescence detection of Hg(2+) in aqueous s...
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PMID: 20859604
PDF is available here.
PDF is available here.
Abstract
A new fluorescent probe (5-[(4-diethylamino-2-hydroxy-benzylidene)-amino]-1H-pyrimidine-2, 4-dione) (Receptor 1) has been synthesized by the Schiff base condensation of 5-aminouracil with 4-(diethylamino)salicylaldehyde. The receptor 1 exhibits high selectively for Al(3+) in DMSO as well as in aqueo...
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PMID: 20820652
PDF is available here.
PDF is available here.
Organic Letters (12)21 2010
Abstract
A novel fluorescent probe, HKGreen-3, for sensing peroxynitrite is designed on the basis of the rhodol scaffold and a peroxynitrite-specific oxidation reaction. The probe turns out to be highly sensitive and selective for detecting peroxynitrite in both chemical and biological system...
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PMID: 20919741
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)45 2010
Abstract
We observed that RNA interference of mucolipin-1 gene (TRPML1) in HEK-293 cells mimics the MLIV cell phenotype consisting of large lysosomes and membranous vacuoles that accumulate chelatable zinc. To show that abnormal chelatable zinc levels are indeed correlated with MLIV pathology, we quantified...
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PMID: 20864526
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)45 2010
Abstract
We found that MitoQ is 30-fold more cytotoxic to breast cancer cells than to healthy mammary cells. MitoQ treatment led to irreversible inhibition of clonogenic growth of breast cancer cells through a combination of autophagy and apoptotic cell death mechanisms. Relatively limited cytotoxicity was s...
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PMID: 20805228
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)45 2010
Abstract
We searched for novel ECE-specific peptide substrates. To this end, peptides derived from the big endothelin intermediate were tested using ECE-1 and ECE-2, leading to the identification of an ECE-1-specific substrate. Moreover, screening of our proprietary fluorigenic peptide Fluofast® libraries u...
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PMID: 20807771
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)45 2010
Abstract
We examined the effect of indomethacin on membrane lateral heterogeneity. Fluorescence lifetime imaging of cells expressing lipid-anchored probes revealed that treatment of BHK cells with therapeutic levels of indomethacin enhances cholesterol-dependent nanoclustering, but not cholesterol-independen...
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PMID: 20826816
PDF is available here.
PDF is available here.
Abstract
Interactions between different types of immune cells and organically-modified silica nanoparticles were studied. The silica particles functionalized with amine groups were prepared by sol-gel technique. Sheep immunoglobulin labeled with fluoresceine isothiocyanate was immobilized by adsorption onto...
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PMID: 20648538
PDF is available here.
PDF is available here.
Abstract
To evaluate the utility of intrathecal fluorescein (IF) for intraoperative localization and successful repair of cerebrospinal fluid (CSF) leaks.
Case series with chart review.
Tertiary-care medical center.
Subjects included those undergoing endoscopic CSF leak repair with or without the use of IF....
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PMID: 20974330
PDF is available here.
PDF is available here.
Parasitology (137)13 2010
Abstract
Cryptosporidium parvum, belonging to the phylum Apicomplexa, is a major cause of waterborne gastroenteritis throughout the world. The sporozoites are thought to invade host enterocytes using an active process termed gliding motility. However, the biological and morphological changes within the sporo...
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PMID: 20800015
PDF is available here.
PDF is available here.
Abstract
White petrolatums of Japanese Pharmacopoeia grade and Sun white marketed as a cosmetic were characterized by measuring their physical properties and drug-releasing characteristics. White petrolatums of Japanese Pharmacopoeia grade available commercially in Japan were Perfecta, White 1S, Ultima, Snow...
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PMID: 21155385
PDF is available here.
PDF is available here.
Abstract
We have used phagocytosis as a paradigm of signal transduction, taking advantage of the generous size of phagosomes and of their comparatively leisurely rate of formation. Aided by the design of specific probes, we demonstrated a highly localized and elegantly choreographed sequence of changes in th...
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PMID: 20739621
PDF is available here.
PDF is available here.