Abstract
A novel approach for selectively encapsulating Cy5 dye molecules via DNA hybridization in cationic phosphorus dendrimers (G(4)(+))/polystyrenesulfonate (PSS) microcapsules has been developed. The hybridization between Cy5 labeled single strand DNA (ssDNA) targets and complementary ssDNA probes was p...
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PMID: 20844779
PDF is available here.
Abstract
We have developed a nanoparticle (NP)-conjugated FRET probe based on the human estrogen receptor α (ER) ligand-binding domain (LBD) to detect phytoestrogens. The NP-conjugated FRET probe showed fluorescence signals for genistein, resveratrol and daidzein compounds with Δ ratios of 1.65, 2.60 and 1...
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PMID: 20877819
PDF is available here.
Abstract
A novel chemiluminescent (CL) technique for the rapid determination of proteins on a membrane is described. The method utilizes an interaction between luminol-labeled alginic acid macromolecule and proteins. The synthesis of the macromolecular probe consists of the oxidation of alginic acid with NaI...
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PMID: 20865196
PDF is available here.
Abstract
Herein, a sensitive and selective sensor for mercury(II) and glutathione based on the aggregation-induced emission (AIE) of a tetraphenylethene derivative stimulated by Hg(2+)-DNA complexes is reported. Aggregation complexes of AIE probes, quaternized tetraphenylethene salt and anti-Hg(2+) aptamer s...
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PMID: 20877906
PDF is available here.
Abstract
We have developed a microfluidics based platform and methodology named MAPS (microfluidic system for analyzing proteins in single complex) for detecting two protein interactions rapidly using a single fluorophore. Target proteins were labelled with Quantum dot 525 (QD525) via specific polyclonal ant...
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PMID: 20877860
PDF is available here.
Abstract
Electrochemiluminescence (ECL) derived from Ru(bpy)(3)(2+) and its derivatives has been proven to be useful in diverse areas of analytical chemistry. Up to date most of the applications are based on coreactant ECL technology. However, little attention has been paid to the ECL inhibition between core...
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PMID: 20820476
PDF is available here.
Abstract
We faced such challenges in our efforts to implement an HTS for inhibitors of the phosphopantetheinyl transferase Sfp using an assay that had been validated using commercially available reagents. Here we demonstrate a facile route to the synthetic preparation of reactive tetraethylrhodamine and quen...
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PMID: 20725690
PDF is available here.
Abstract
Three carbazole dicationic salts, namely 3,6-bis(1-methyl-4-vinylpyridinium) carbazole diiodide (BMVC), 9-ethyl-3,6-bis(1-methyl-4-vinylpyridinium) carbazole diiodide (9E-BMVC) and 9-ethyl-3,6-bis(1-hydroxyethyl-4-vinylpyridinium)carbazole diiodide (9E-BHVC), were synthesized successfully. Their pho...
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PMID: 20714660
PDF is available here.
Abstract
A highly sensitive and selective 1,8-naphthyridine-based fluorescent chemodosimeter for Zn(2+) has been designed, synthesized, and applied to the detection of intracellular Zn(2+).
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PMID: 20820508
PDF is available here.
Abstract
We describe the development of a fluorescence polarization-Activity Based Protein Profiling (fluopol-ABPP) based high throughput screening assay that can be used to identify PAD-selective inhibitors. Using this assay, streptonigrin was identified as a potent, selective, and irreversible PAD4 inactiv...
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PMID: 20740228
PDF is available here.
Abstract
A novel NAT2 specific probe has been developed for sensitive detection of the activity of NAT2. Upon NAT2 catalyzed acetylation of the molecule to N-acetyl-amonafide, the ratiometric response of fluorescence with significant changes in wavelength and intensity is observed. Its significant applicatio...
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PMID: 20798904
PDF is available here.
Abstract
A small glutamine donor substrate was designed for single-step and site-specific protein multifunctionalization catalyzed by microbial transglutaminase.
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PMID: 20820527
PDF is available here.
Abstract
A fluorescent probe for mercury(II) ions, based on the quenching of fluorescence of terbium(III) ions doped in CdS nanoparticles, has been developed. The terbium(III)-doped cadmium sulfide composite nanoparticles were successfully synthesized through a straightforward one-pot process, with the biomo...
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PMID: 20663708
PDF is available here.
Abstract
A novel beta-cyclodextrin (beta-CD) sensitized fluorescence quenching method for the determination of l-tyrosine (l-Tyr) with Mo(VI)-phenyl-fluorone (PF) as a fluorescence probe has been developed. The fluorescence intensity of Mo(VI)-PF-beta-CD was diminished as the l-tyrosine was added, the fluore...
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PMID: 20667765
PDF is available here.
Tae Il TI Son,
Makoto M Sakuragi,
Sawa S Takahashi,
Sei S Obuse,
Jeonghwa J Kang,
Masako M Fujishiro,
Haruhiko H Matsushita,
Jiansheng J Gong,
Shigeru S Shimizu,
Yusuke Y Tajima,
Yasuhiro Y Yoshida,
Kazuomi K Suzuki,
Toshio T Yamamoto,
Mariko M Nakamura and
Yoshihiro Y Ito
Abstract
A novel visible light-crosslinkable porcine gelatin was prepared for gelation and micropatterning. The preparation employed a photo-oxidation-induced crosslinking mechanism. First, furfuryl groups were incorporated into the gelatin. Second, the modified gelatin was mixed in water with Rose Bengal, w...
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PMID: 20580950
PDF is available here.
Abstract
I report highly emissive pyrene-based fluorophores and highly sensitive fluorescent sensors using pyrene emission switching. Alkynylpyrene derivatives show fruitful photophysical properties, as compared with pyrene itself: longer absorption and emission wavelengths and high fluorescence quantum yiel...
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PMID: 20930479
PDF is available here.
Abstract
To investigate the cellular uptake and elimination process of a lipophilic drug loaded in different nanocarriers, emulsions, liposomes and poly (DL-lactide-co-glycolide) (PLGA) nanoparticles loaded with a model drug, coumarin 6, were prepared and their transportation in HeLa cells compared. After 4...
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PMID: 21105575
PDF is available here.
Abstract
Two oxime-based colorimetric probes for the hypochlorite anion (ClO(-)) have been rationally designed and synthesized on basis of the mechanism of intramolecular charge transfer (ICT). Upon addition of ClO(-), the probes displayed around 20nm redshift in the absorption maximum, accompanied with the...
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PMID: 20591725
PDF is available here.
Abstract
We describe the use of human SUMO1 and SUMO2 as a useful gene fusion technology. Human SUMO1 and SUMO2 fusion expression vectors were constructed and tested in His-tag and ubiquitin fusion expression systems. Two difficult-to-express model proteins, matrix metalloprotease-13 (MMP13) and enhanced gre...
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PMID: 20457256
PDF is available here.
Abstract
4-Hydroxyphenylmethylene hydantoin (PMH, 1), isolated from the Red Sea sponge Hemimycale arabica, and delta-tocotrienol (3), isolated from the tocotrienol-rich fraction of palm oil, are important antimetastatic and antiproliferative natural products that proved effective against metastatic prostate...
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PMID: 21121261
PDF is available here.
Abstract
We demonstrate that the use of an internal standard helps to account for technical variation caused by spatial intensity biases that exist in the gels and propose a novel data-preprocessing technique, a spatial intensity bias removal (SIBR), which can approximate these biases using only the data of...
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PMID: 20681517
PDF is available here.
Abstract
We investigated whether fluorescent agents, especially vitamin B2, can act as tracers for intraoperative pulmonary sentinel node mapping.
Vitamin B2, fluorescent beads, and green fluorescent protein (GFP) were each injected into pulmonary parenchyma in 4 pigs (experiment 1). The safety of each trace...
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PMID: 21036727
PDF is available here.
Abstract
We investigated the rate of the leakage of various sized fluorescent probes through the magainin 2-induced pores in single giant unilamellar vesicles (GUVs) of 50% dioleoylphosphatidylglycerol (DOPG)/50% dioleoylphosphatidylcholine (DOPC) membrane. Magainin 2- induced leakage of Texas-Red dextran 10...
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PMID: 20799752
PDF is available here.
Abstract
We report the development of a multimodal probe that combines the optical properties of silicon quantum dots with the superparamagnetic properties of iron oxide nanoparticles to create biocompatible magnetofluorescent nanoprobes. Multiple nanoparticles of each type are coencapsulated within the hydr...
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PMID: 20738120
PDF is available here.