Abstract
We state that the in vitro bovine corneal epithelial primary culture model is useful for ocular studies....
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PMID: 21928720
PDF is available here.
Abstract
We developed a fluorescent probe-assisted microscopy protocol enabling to assess the ΔΨ(m)-generating capacity by mitochondria immobilized on a glass surface at the single organelle resolution-level. The advantage of this assay over others is to scale-down the amount of the biological sample requi...
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PMID: 21762790
PDF is available here.
Abstract
We discuss, production, characterization and in-vivo evaluation of nevirapine nanosuspensions. Laser diffraction showed that the average particles size was 457 nm. Following single-dose administration, the plasma gamma concentration profiles showed fast release. Macrophage uptake studies confirmed e...
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PMID: 21699078
PDF is available here.
Abstract
We evaluated the activity of TrxR in adriamycin-resistant leukemic cells (K562/ADM) and adriamycin-sensitive parental lines (K562), and found that TrxR activity was higher in the drug resistant cell sublines K562/ADM than in K562 drug sensitive parental cells. Auranofin, a gold(I) compound clinicall...
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PMID: 21699084
PDF is available here.
Paula F PF Martinez,
Katashi K Okoshi,
Leonardo A M LA Zornoff,
Silvio A SA Oliveira,
Dijon H S DH Campos,
Aline R R AR Lima,
Ricardo L RL Damatto,
Marcelo D M MD Cezar,
Camila C Bonomo,
Daniele M DM Guizoni,
Carlos R CR Padovani,
Antonio C AC Cicogna and
Marina P MP Okoshi
Abstract
Two sets of Sham and infarcted rats were studied. The first was used to perform cluster analysis and the second to prospectively validate the results. Six months after inducing myocardial infarction (MI), rats were subjected to transthoracic echocardiography. Infarct size was measured by histologica...
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PMID: 21617080
PDF is available here.
Abstract
We found that ITCs induces SW480 cells apoptosis in a dose-dependent manner by using MTT assay, phase contrast microscope and flow cytometry, and the IC50 was calculated to be 77.72 microg/ml, superior to the chemotherapeutical drug 5-flurouracil. Subsequently, 15 altered proteins in ITCs treated SW...
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PMID: 21699074
PDF is available here.
Abstract
The aim of this study was to compare the cytotoxic effects of expired and non-expired resin-based composite materials at two, five and seven days on L929 cells. Disc-shaped test samples of expired and non-expired composite materials were prepared according to manufacturers' instructi...
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PMID: 21735869
PDF is available here.
Abstract
We determined the effects of arbutin on TCCSUP human bladder carcinoma cell proliferation. Arbutin did not exhibit any cytotoxic effects in TCCSUP cells at concentrations of < 500 microg/ml. To determine the effects of arbutin on cell proliferation, TCCSUP cells were treated with arbutin at various...
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PMID: 21612160
PDF is available here.
Abstract
The main objective of present research study was to evaluate the potential of lipid nanoparticles for active delivery of an antiretroviral drug to lymphatic tissues. Stavudine entrapped drug loaded solid lipid nanoparticles (SLNs) were prepared and characterized for a variety of phys...
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PMID: 21612153
PDF is available here.
Abstract
Tattoos are very popular. Widely used standard industrial pigments are used. The field lacks basic knowledge concerning pigments, epidemiology and complications such as allergy, granulomas, skin cancer and foreign body reactions. The risks associated with laser treatment are unknown. Studies have su...
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PMID: 21199619
PDF is available here.
Abstract
A case of a 15 year-old girl who developed an erythema multiforme-like reaction to para-phenylenediamine (PPD) after Henna tattoos is described. The patient was treated successfully with oral prednisolone and highly potent topical corticosteroids. The patient developed a long-lasting post-inflammato...
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PMID: 21199625
PDF is available here.
Abstract
Our study shows that Annexin V-CdTe-conjugated polymer can differentiate the motor and sensory nerve fascicles of the peripheral nerve rapidly and precisely in vitro. This technique represents a new method for the identification of peripheral nerve fascicles....
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PMID: 21743158
PDF is available here.
Abstract
Redox-dependent thylakoid protein phosphorylation regulates both the short- and long-term acclimation of the photosynthetic apparatus to changes in environmental conditions. The major thylakoid phosphoproteins belong to photosystem II (D1, D2, CP43, PsbH) and its light-harvesting antenna (Lhcb1, Lhc...
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PMID: 20960130
PDF is available here.
Abstract
Recycling of secondary raw materials is a priority of waste handling in the countries of the European community. A potentially important secondary raw material is the product of the thermal transformation of cement-asbestos, produced by prolonged annealing at 1200-1300 °C. The product is chemically...
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PMID: 20708915
PDF is available here.
Abstract
With the expansion of super-resolution fluorescence microscopy methods, it is now possible to access the organization of cells and materials at the nanoscale by optical means. This review discusses recent progress in super-resolution imaging of isolated and cell DNA using single-molecule localization...
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PMID: 21184489
PDF is available here.
Abstract
Oospores are the most persistent propagules of Phytophthora capsici. The viability of oospores is determined by staining methods such as the tetrazolium bromide (MTT) test.
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PMID: 21167295
PDF is available here.
Abstract
This communication describes a simple method for printing aqueous solutions with millimeter-scale patterns on a variety of substrates using an easily fabricated, paper-based microfluidic device (a paper-based "stamp") as a contact printing device. The device is made from inexpensive materials, and i...
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PMID: 20949218
PDF is available here.
Abstract
A direct molding method for creating a homogeneous, polymer microfluidic channel is presented. By utilizing capillary rise and subsequent absorption of poly(methyl methacrylate) (PMMA) solution into a solvent-permeable poly(dimethyl siloxane) (PDMS) mold, various circular or elliptic polymer microch...
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PMID: 20938498
PDF is available here.
Abstract
Pesticides are used to protect plants all over the world. Their increasing specificity has been due to utilization of differences in biochemical processes, and has been accompanied by lower human toxicity. Nevertheless cases of poisoning are still observed. While certain toxic substances are provide...
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PMID: 20467839
PDF is available here.
Abstract
To examine the prognostic implications of routine elastica staining for venous invasion on prediction of cancer-specific survival in colorectal cancer.
Venous invasion is an important high risk feature in colorectal cancer, although prevalence in published studies ranges from 10% to 90%. To resolve...
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PMID: 21107109
PDF is available here.
Abstract
We tested the hypothesis that the BBB is disrupted by HCA.
Forty-one piglets (mean weight 11 kg) were randomly allocated to acute and survival experiments. Five groups (25 piglets, 5 per group) underwent acute studies: anesthesia alone (control); CPB at 37°C with full-flow (FF); CPB at 25°C with v...
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PMID: 21095352
PDF is available here.
Abstract
Astaxanthin (AST) is a powerful antioxidant that occurs naturally in a wide variety of living organisms. Much experimental evidence has proved that AST has the function of eliminating oxygen free radicals and can protect organisms from oxidative damage. The present study was carried out to further i...
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PMID: 20846510
PDF is available here.
Abstract
Two new heterobimetallic phenylmercury(ii) dithiocarbamate complexes incorporating the ferrocenyl moiety (C(5)H(5))Fe(C(5)H(4)) (Fc), namely PhHgS(2)CN(CH(2)Fc)CH(2)C(5)H(4)N, (1) and PhHgS(2)CN(CH(2)Fc)CH(2)C(4)H(3)O, (2) have been prepared and characterized by elemental analysis, UV-Vis, IR, (1)H...
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PMID: 20830401
PDF is available here.
Abstract
We survey methods of optimizing the optical set-up, enhancing the β of the dye and achieving biological compatibility. In conclusion, we examine novel applications of SHG imaging and highlight promising directions for the development of the field....
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PMID: 20820473
PDF is available here.
Abstract
Intraoperative fluorescence angiography has been reported to be a promising method, with rapid and high-quality image production at low cost when used for the detection of microvascular complications. The purpose of this study was to evaluate the reliability of intraoperative near-infrared indocyani...
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PMID: 21042107
PDF is available here.
Abstract
I error of 0.017. Six milligrams per injection provided a similar contrast-to-background ratio but also a higher residual background signal.
Based on this pilot study, the authors conclude that near-infrared assessment of perforator flap breast reconstruction is feasible with a light-emitting diode-...
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PMID: 21042103
PDF is available here.
Abstract
Perforator flaps have become increasingly popular in reconstructive surgery, as patients experience less donor-site morbidity than with conventional musculocutaneous flaps. Previously, the authors' laboratory described the intraoperative use of near-infrared fluorescence angiography for patient-spec...
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PMID: 21042109
PDF is available here.
Abstract
To investigate the protective effects of citalopram on neural cells and its possible mechanism.
Rat PC12 cells were induced with 100 mg/L nerve growth factor for 7 d before they were randomly divided into following groups: serum-free group, citalopram treatment groups (20, 40, 60, 80 and 100 micromo...
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PMID: 21155393
PDF is available here.
Abstract
We propose a biological cortical column model, at an intermediate mesoscopic scale, in order to better understand and interpret biological sources of voltage-sensitive dye imaging signal (VSD signal). To perform a quantitative analysis of the relative contributions to the VSD signal, a detailed comp...
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PMID: 20600993
PDF is available here.
Abstract
I - 72 patients/111 lesions) versus magnification colonoscopy with targeted indigo carmine dye (Group II - 72 patients/137 lesions) was performed in consecutive patients with lesions 1 cm or less. Histopathology of the specimens was regarded as the gold standard.
In group I, 86 (77.5%) lesions showe...
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PMID: 20453654
PDF is available here.
Abstract
Approximately 15% of acute coronary syndrome (ACS) cases have no significant coronary stenosis. Mechanisms underlying the attacks are, however, unknown.
The clinical study had 254 patients with ACS; 38 patients (31 females and 7 males; aged 51.0 ± 8.0 years) had no significant coronary stenosis on...
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PMID: 20827027
PDF is available here.
Abstract
The base specificity and energetics of DNA binding of the phenazinium dyes phenosafranine and safranine-O have been studied using various biophysical tools. The guanine-cytosine base specificity of both compounds was established from binding affinity values and competition dialysis results and also...
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PMID: 20730145
PDF is available here.
Abstract
Our group demonstrated that amyloid formation by amyloid β-protein (Aβ) was facilitated by gangliosides in lipid raft-like model membranes. Phosphatidylserine and phosphatidylglycerol were also reported to trigger fibril formation by human islet amyloid polypeptide (hIAPP). However, it is not veri...
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PMID: 20930481
PDF is available here.
Abstract
A new high-sensitivity detection of protein assay at the nanogram level is proposed based on multi-spectroscopic methods including resonance light scattering (RLS), atomic force microscopy (AFM), ultraviolet spectra (UV) and fluorescence spectra etc. Under the optimum conditions, the amplified RLS s...
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PMID: 20638325
PDF is available here.
Abstract
To evaluate the stability of the interfacial layer and leakage patterns between enamel, DEJ, dentin and three adhesive systems as demonstrated by either a dye or a silver nitrate technique to understand the marginal integrity of restored tooth substrates.
Extracted human molars were cross-sectioned...
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PMID: 21207797
PDF is available here.
Abstract
A malayaite pigment was synthesized with wastewater containing low chromium (Cr)(VI) by a novel liquid-phase reaction method. The effects of the particle size of the precursor and calcination temperature on the final product are examined. The result of scanning electron microscopy shows that the obt...
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PMID: 21090553
PDF is available here.
Abstract
In this paper the Mannich reaction product of dimethylamine, acetone and formaldehyde was grafted onto hydroxymethylated lignin to form a lignin-base cationic polyelectrolyte (L-DAF). L-DAF samples with different cationic strengths and molecular weights were synthesized and character...
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PMID: 20576562
PDF is available here.
Abstract
This work presents role of different enzymes in decolorization of industrial dye Orange T4LL by Bacillus sp. VUS. Bacillus sp. strain VUS decolorized dye Orange T4LL, under static anoxic condition in 24 h. During decolorization of Orange T4LL a significant induction in the activities of lignin perox...
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PMID: 20655588
PDF is available here.
Abstract
These results suggest that phloroglucinol can provide a therapeutic potential for prevention of chronic inflammation.
Copyright (c) 2010 Elsevier Ltd. All rights reserved....
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PMID: 20667461
PDF is available here.
Abstract
The antioxidant and pro-oxidant potential of an extract from red radish, in which the major compounds were acylated pelargonidin derivatives, were assessed with a variety of assays in vitro. The extract appeared to form a complex with Fe(3+) or Cu(2+). It displayed a concentration-dependant reducing...
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PMID: 20600520
PDF is available here.
Abstract
Modified Alcian blue stain is a relatively rapid, cheap, highly sensitive, and specific method of detecting metastatic invasive lobular carcinoma. This method can be used in conjunction with conventional staining methods used intraoperatively....
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PMID: 20923308
PDF is available here.
Abstract
We report the case of a 67-year-old asymptomatic man who had a 1.3-cm, incidentally detected, pancreatic tail mass. The mass was round, well-circumscribed, and hypervascular with uniform enhancement. The image findings were highly suggestive of a pancreatic endocrine neoplasm. An endoscopic ultrasou...
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PMID: 20923303
PDF is available here.
Abstract
We present a new and general scheme for analytical applications of droplet-based microfluidics in which flowing droplets function not only as isolated reaction flasks, but are also capable of on-drop separation and sensing. To demonstrate this, we choose ionic liquids as designer fluids whose chemic...
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PMID: 20697661
PDF is available here.
Abstract
The eukaryotic cell has evolved to compartmentalize its functions and transport various metabolites among cellular compartments. Therefore, in cell biology, the study of organization and structure/function relationships are of great importance. The endoplasmic reticulum is a highly convoluted, singl...
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PMID: 20810627
PDF is available here.
Abstract
Liposomes applications in health care include meanly their ability to carry drugs and genes inside the human body for therapeutic purposes. Nevertheless their applicability can extend far beyond and could be used as analytical tools in order to perform rapid, low-cost, sensitive and...
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PMID: 21133073
PDF is available here.
Abstract
We selected a beta-glucosidase from Aspergillus niger, which shows a high hydrolytic activity towards indican and was thermally stable at temperatures up to 50-60 degrees C, in an acidic pH region. The A. niger beta-glucosidase, when immobilized on Chitopearl BCW-3001 by treatment with glutaraldehyd...
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PMID: 20547334
PDF is available here.
Abstract
Skin-sparing mastectomy has been associated with flap ischemia and necrosis. Current clinical methods for assessment of flap viability following mastectomy are largely subjective and lack objective data to guide intraoperative decisions. Intraoperative laser-assisted indocyanine green angiography (L...
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PMID: 20539977
PDF is available here.
Abstract
Nanoparticles of zinc oxide semiconductor were applied for removal of toxic organic pollutants such as dyes (alizarin yellow GG) from wastewater using laser induced photocatalytic process. A special photoreactor was designed for this purpose using local resources. Laser enhanced photo degradation of...
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PMID: 20694880
PDF is available here.
Abstract
The combination of NV1023 and radiation yielded a synergistic oncolytic effect in all tested pancreatic cancer cell lines, with the greatest effect achieved in MIA PaCa-2. This effect was not mediated by an increase in rapid viral replication, but by a substantial increase in apoptosis. CONCLUSION:...
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PMID: 20629009
PDF is available here.
Abstract
Fluorescent cholangiography delineated the cystic duct in all 52 patients, and the cystic duct-common hepatic duct junction was visible before dissection of Calot's triangle in 50 patients. Fluorescent imaging also identified all accessory bile ducts that had been diagnosed before surgery in eight p...
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PMID: 20623766
PDF is available here.
Abstract
In this study, the microstructure of the cornea was compared among chickens (Gallus gallus), jungle crows (Corvus macrorhynchos), rats (Rattus norvegicus) and rabbits (Oryctolagus cuniculus). The density of keratocytes in the mammals was over 3 times that in the birds. The size of the keratocytes in...
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PMID: 20410677
PDF is available here.
Abstract
We collected 210 human serum samples and tested them by the dye test using in vivo tachyzoites (conventional method) then compared these results with three other methods: a dye test using cell culture-derived T. gondii tachyzoites and indirect immunofluorescent antibody tests (IFAT) using in vivo an...
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PMID: 21073025
PDF is available here.
Abstract
The purpose of this study was to evaluate the efficacy of verteporfin photodynamic therapy on the treatment of polypoidal choroidal vasculopathy.
A prospective, nonrandomized institutional study was conducted involving 42 eyes of 38 patients with newly diagnosed symptomatic polypoidal choroidal vasc...
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PMID: 20827139
PDF is available here.
Abstract
We describe the distribution of elastic system fibers in the superficial layer of the lamina propria of Reinke's edema, as compared with normal vocal folds.
Weigert's resorcin-fuchsin stain after oxidation with 10% oxone was used to study the arrangement of elastic fibers. The findings were categori...
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PMID: 21033028
PDF is available here.
Abstract
Parasympathetic control of the heart is an important component in the regulation of normal cardiac function. However, the anatomic course of parasympathetic innervation of the heart is unclear.
The purpose of this study was to apply a gross parasympathetic nerve stain technique to reveal the details...
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PMID: 20381645
PDF is available here.
Abstract
In a screening of saprotrophic, ectomycorrhizal and plant pathogen ascomycetes a frequent occurrence of laccase was observed. Lamprospora wrightii, the best producing organism, was chosen to elucidate the properties of a laccase from a moss-associated, saprotrophic ascomycete. The ex...
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PMID: 20652905
PDF is available here.