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Chromogenic Compounds (0):
Recent article
Chromogenic Compounds
Abstract
A novel 1,3,5-substituted triethylbenzene derivative with a 2-aminobenzimidazole moiety as a binding and signaling subunit was synthesized. The sensor was tested in a buffered CH(3)CN/H(2)O (99:1, v/v) solution and found to be selective for iodide as demonstrated by the photophysical...
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PMID: 21574613
PDF is available here.
PDF is available here.
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Abstract
We studied 79 isolates, in which the most prevalent species using the system ID32C and PCR was C. albicans, followed by C. tropicalis, C. glabrata and C .krusei. PCR patterns obtained for the identification of clinical isolates were stable and consistent in the various independent studies and showed...
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PMID: 21167300
PDF is available here.
PDF is available here.
Thrombosis and Haemostasis (104)3 2010
Abstract
I was 13 +/- 2 microM. k cat was seven fold decreased whereas K M was unaffected in the presence of 10 microM Zn2+. The inhibitory effect of Zn2+ on APC activity was also observed when factor Va was used as a substrate in an assay coupled to a prothrombinase assay. The interaction of Zn2+ with APC w...
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PMID: 20664913
PDF is available here.
PDF is available here.
Haemophilia (16 Suppl 5) 2010
Abstract
Abstract
We have compared a chromogenic medium, Brilliance Candida Agar, with CHROMagar Candida, the chromogenic medium most used in our country. A multicentre study was conducted in 16 Hospitals belonging to the Mycology Net of Buenos Aires City Government. A total of 240 yeast isolates were included in thi...
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PMID: 20346288
PDF is available here.
PDF is available here.
Abstract
In this work, hollow silica microspheres (HSMs), which have hollow interiors and nanoporous shells, were used in the immobilization of enzyme to establish a novel chemiluminescence biosensor. The immobilization behavior of enzyme in HSMs with different pore sizes has been studied. The results reveal...
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PMID: 20185291
PDF is available here.
PDF is available here.
Abstract
We have used gold nanoparticles (AuNPs) to covalently attach an antibody (Ab(1)) using a spacer arm. The AuNPs/Ab(1) modified gold electrode was used for a sandwich electrochemical immunoassay. The detection was done using cyclic voltammetry and impedance measurements using Horse Radish Peroxidase (...
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PMID: 20171869
PDF is available here.
PDF is available here.
Analytica Chimica Acta (667)1-2 2010
Abstract
We have investigated the potential of a capillary-flow hydrothermal UV-vis spectrophotometer (CHUS) for performing in situ measurements of proteins and determining their behavior at extremely high temperatures, in combination with a chromogenic reagents probe, which interacts with the proteins. The...
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PMID: 20441871
PDF is available here.
PDF is available here.
Abstract
The objective of this study was to investigate the effects of glucose-based peritoneal dialysis (PD) fluids and icodextrin-based PD fluids on the expression of Toll-like receptor 2 (TLR2)/TLR4 and subsequent ligand-induced mitogen-activated protein kinase (MAPK) and NF-kappaB signaling and tumor nec...
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PMID: 20200188
PDF is available here.
PDF is available here.
Abstract
Foot wounds are the most common diabetes-related cause of hospitalization and frequently result in amputation. Although generally diagnosed clinically based on signs and symptoms of inflammation, empirical antibiotic treatment should be based on tissue cultures until resolution of in...
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PMID: 20207621
PDF is available here.
PDF is available here.
Abstract
Foot wounds are the most common diabetes-related cause of hospitalization and frequently result in amputation. Although generally diagnosed clinically based on signs and symptoms of inflammation, empirical antibiotic treatment should be based on tissue cultures until resolution of in...
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PMID: 20207621
PDF is available here.
PDF is available here.
Abstract
The overall purpose of the study was to demonstrate applicability of the DAKO dual-color chromogenic in situ hybridization (CISH) assay (DAKO Denmark, Glostrup) with respect to 4 fluorescence in situ hybridization (FISH) probes: MYC (c-MYC), EGFR, ERBB2 (HER2), and TOP2A. The study showed that the d...
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PMID: 20093229
PDF is available here.
PDF is available here.
Abstract
We adapted the highly productive T7 expression system to S. lividans. A codon-optimized T7 RNA polymerase gene was chromosomally integrated, and a bifunctional T7 expression vector was constructed....
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PMID: 20023105
PDF is available here.
PDF is available here.
Clinical laboratory (56)11-12 2010
Abstract
Methicillin-Resistant Staphylococcus aureus (MRSA) is one of the most common and important causes of nosocomial infections. Rapid detection of this pathogen is important for conducting good and swift infection control. This prospective study evaluates two chromogenic media for the detection of MRSA....
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PMID: 21141444
PDF is available here.
PDF is available here.
Abstract
For all tested strains, the chromID ESBL agar showed a high sensitivity (97%), but a low specificity (66%). The positive and negative predictive values were 98% and 57%, respectively. The chromID ESBL had false-positive results for 2 confirmed ESBL-positive strains. CONCLUSION: The very good sensiti...
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PMID: 20700996
PDF is available here.
PDF is available here.
Caries Research (44)1 2010
Abstract
Models to evaluate the anticaries potential of fluoride (F) formulations containing monofluorophosphate (MFP) should consider the release of F ion to the oral environment by its enzymatic hydrolysis. This was tested in situ, using a test plaque of a strain of Streptococcus mutans which presents high...
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PMID: 20110697
PDF is available here.
PDF is available here.
Abstract
We found that Spectra MRSA was able to rapidly identify and differentiate methicillin-resistant S. aureus from methicillin-susceptible S. aureus on the basis of the utilization of chromogens that result in denim blue colonies, thus eliminating the need for biochemical analysis and antimicrobial susc...
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PMID: 19889898
PDF is available here.
PDF is available here.
Abstract
Maintaining a balance between bleeding and clotting has always been a challenge in treating coagulation disorders. A perturbation in that balance can be associated with substantial morbidity and mortality. As a result, anticoagulant monitoring is extremely important, and inappropriat...
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PMID: 20131309
PDF is available here.
PDF is available here.
Abstract
A fluorescent and colorimetric probe bearing salicylaldehyde hydrazone functionality has been prepared for cyanide sensing. The detection of cyanide was performed via the nucleophilic attack of cyanide anion on the imine group of the probe with a 1:1 binding stoichiometry, which could be confirmed b...
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PMID: 19836585
PDF is available here.
PDF is available here.
Abstract
Thrombosis and Haemostasis (102)5 2009
Abstract
We describe here a new optical molecular probe for the detection of LMWH in serum samples. The polycationic ruthenium compound 1 is applicable to the quantification of heparin by monitoring 630 nm fluorescence. In addition, compound 1 is a rare example of a non-polymeric low molecular weight compoun...
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PMID: 19888520
PDF is available here.
PDF is available here.
Thrombosis and Haemostasis (102)5 2009
Abstract
We compared the dose response of four conventional LMWHs and fondaparinux in normal plasma, and plasma from women in first, second and third trimester of pregnancy. Three concentrations of LMWHs, fondaparinux, or unfractionated heparin were added to pooled plasma samples from non-pregnant women (n=1...
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PMID: 19888540
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)40 2009
Abstract
We now demonstrate that an Ile-Ile or Ile-Val dipeptide can induce limited enzyme activity in the single-chain zymogen form of urokinase-type plasminogen activator (uPA) or its K158A variant, which cannot be activated proteolytically. Furthermore, the slow formation of a covalent serpin-protease com...
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PMID: 19705874
PDF is available here.
PDF is available here.
Biotechnology Letters (31)8 2009
Abstract
An image analysis-based method for high throughput screening of an alpha-amylase mutant library using chromogenic assays was developed. Assays were performed in microplates and high resolution images of the assay plates were read using the Virtual Microplate Reader (VMR) script to quantify the conce...
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PMID: 19360387
PDF is available here.
PDF is available here.
Seminars in Hematology (46)3 2009
Abstract
We briefly review important advances in molecular microbiology, and then discuss specific assays that have been implemented in clinical microbiology laboratories throughout the country. We also discuss emerging methods and technologies that will soon be more widely used for the prompt and accurate d...
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PMID: 19549577
PDF is available here.
PDF is available here.
Abstract
The aim of the present study was to evaluate a new one-step enrichment protocol, consisting of a combined preenrichment and enrichment broth (Cronobacter Enrichment Broth [CEB]) used in conjunction with selective-differential agar ChromID Sakazakii, to facilitate a shortened 2-day cultural method fo...
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PMID: 19681272
PDF is available here.
PDF is available here.
Archives of Oral Biology (54)8 2009
Abstract
We evaluated the in vitro effects of LIPUS on HGF proliferation and differentiation to test its feasibility for periodontal therapy.
LIPUS treatment (1.5MHz, 30mW/cm(2)) was applied to HGF in the experimental groups after 24-h of culture (5 or 10min/day for 28 days) and omitted in th...
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PMID: 19493525
PDF is available here.
PDF is available here.
Abstract
Analytical Biochemistry (390)1 2009
Abstract
Efficient use of xylose is necessary for economic production of biochemicals and biofuels from lignocellulosic materials. Current studies on xylose uptake for various microorganisms have been hampered by the lack of a facile assay for xylose transport. In this work, a rapid in vivo, label-free metho...
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PMID: 19351525
PDF is available here.
PDF is available here.
Abstract
Surveillance for methicillin-resistant Staphylococcus aureus (MRSA) colonization can be an important element for infection control programs when managing a multidrug-resistant pathogen such as MRSA. The sensitivity and speed of laboratory testing affects the proportion of appropriate isolation days...
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PMID: 19289589
PDF is available here.
PDF is available here.
Abstract
We used 226 consecutive breast carcinomas from 2 institutions and tested CISH and FISH on the same tumor set simultaneously at different test sites. Besides manufacturers' scoring criteria, the new American Society of Clinical Oncology/College of American Pathologists (ASCO/CAP) guidelines were used...
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PMID: 19289584
PDF is available here.
PDF is available here.
Abstract
Using standard solutions and in the presence of a drug matrix, Reichardt's dye shows promise as a reagent for detection of low levels of industrially important alkylating agents....
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PMID: 19298702
PDF is available here.
PDF is available here.
Abstract
This study compares the performance of three chromogenic culture agar plates, chromID MRSA, MRSA-Screen and MRSA-Select, by challenging with a collection of Staphylococcus aureus strains and screening samples obtained from hospitalised patients. All chromogenic media showed excellent sensitivity (>9...
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PMID: 18855028
PDF is available here.
PDF is available here.
Abstract
All three media are useful tools for the isolation of VRE from stool samples. AES VRE Agar and bioMérieux chromID VRE are easier to use than Oxoid VRE Agar due to diffusion of black coloration from the latter. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study to evaluate the performance...
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PMID: 19196440
PDF is available here.
PDF is available here.
Abstract
We conclude, that all tested media showed good performance in presumptive identification and detection of resistance mechanisms....
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PMID: 19780495
PDF is available here.
PDF is available here.
Abstract
The ''Rainbow western'' method permits detection of multiple antigens on a single protein blot. The procedure utilizes horseradish peroxidase (HRPase)-based detection with both a chemiluminescent and colorimetric substrate. In the ''Rainbow western'' procedure four different HRPase-colorimetric subs...
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PMID: 19378037
PDF is available here.
PDF is available here.
Abstract
A variation of ECL immunodetection method permits sequential detection of multiple antigens (MAD) on a single protein blot without stripping previously bound antibodies. Because antibody stripping is not involved, immobilized proteins are not lost from the membrane, which permits multiple sequential...
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PMID: 19378084
PDF is available here.
PDF is available here.
Abstract
Invisible ink and writing secret messages have been part of man's fantasy, having proven useful in clandestine and high sensitivity areas. Security inks, made up of invisible materials that give printed images that cannot be photocopied, that can be read only under special environments have become i...
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PMID: 19378093
PDF is available here.
PDF is available here.
Haemophilia (15)1 2009
Abstract
We identified 109 patients with mild haemophilia A amongst whom 92 were eligible to enter the study. These represent a total of 53 unrelated families. Our data illustrate that this assay discrepancy pattern is found quite frequently amongst our mild haemophilia A families. While the ratio of FVIII:C...
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PMID: 19149854
PDF is available here.
PDF is available here.
Clinical laboratory (55)3-4 2009
Abstract
The apparent activities of seven substrates: B3644, S2238, S2251, ChromozymPCa, T6140, ChromozymPK, and T1637, for porcine kallikrein were 0.788, 0.691, 0.659, 0.636, 0.438, 0.396, and 0.145, respectively, that of B3644 being the highest. Amidolysis caused by kaolin activated human normal plasma was...
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PMID: 19462932
PDF is available here.
PDF is available here.
Abstract
Generally, a conventional culture-based examination procedure (detection by egg-yolk salt agar and subsequent identification by phenotypic tests) for confirmation of the presence of S. aureus (SA) in laboratory mice and rats requires approximately 4 days. To improve the culture-based examination pro...
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PMID: 19194073
PDF is available here.
PDF is available here.
Surgical Innovation (15)4 2008
Abstract
Abstract
The European Pharmacopoeia (Ph. Eur.) Biological Reference Preparation (BRP) batch 1, the World Health Organisation (WHO) 3rd International Standard, Human (IS, 96/854) and the FDA Standard for human blood coagulation Factor IX concentrate have been available since 1996, following their establishmen...
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PMID: 19220978
PDF is available here.
PDF is available here.
Abstract
Our findings suggest that the Her-2 status can be reliably assessed on primary tumor but a possible difference can be found in Her-2, EGFR, and cyclin D1 status between the primary and the metastatic sites and this possibility should be concerned in patients considering molecular targeted therapy or...
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PMID: 19119452
PDF is available here.
PDF is available here.
Abstract
A new chromogenic protocol for the selective detection of nerve agent mimics is reported.
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PMID: 19030566
PDF is available here.
PDF is available here.
Abstract
Since the absorption spectra of Fe(II) ions solutions vary with the concentration of Fe(II) irons, the authors tried to establish a direct measurement of active haemachrome in human blood through spectral analysis, and successfully made an experiment on it. The authors prepared Fe(II) ions solutions...
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PMID: 19248515
PDF is available here.
PDF is available here.
Abstract
We compared two automated identification and susceptibility testing systems with regard to their effectiveness in detecting ESBL production in Enterobacteriaceae: the BD Phoenix system (BD Diagnostic Systems, Sparks, MD) and the Vitek 2 system (bioMerieux, Marcy l'Etoile, France). We tested 114 stra...
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PMID: 18845821
PDF is available here.
PDF is available here.
Abstract
Of 1386 urine specimens, the consistencies in clinical significance of CO and CPS3 to routine media were 90.7% and 89.8%, respectively. For the enumeration of microorganisms, 524, 514, and 521 clinically significant isolates were isolated on routine media, CO, and CPS3, respectively. Of the 524 sign...
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PMID: 19122925
PDF is available here.
PDF is available here.
Abstract
A cleaner and simple spectrophotometric method using microflow analysis (muFA) was performed. It consisted of a T-junction with microcoil on a polymethyl methacrylate (PMMA) chip which was fabricated by laser ablation and a molded polydimethylsiloxane (PDMS) as top plate. The fabricated PMMA chip wa...
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PMID: 18065259
PDF is available here.
PDF is available here.
Abstract
Enterobacter sakazakii is an emerging pathogen associated with life-threatening neonatal infections resulting from the consumption of contaminated powdered infant formula milk (IFM). Recent taxonomic analyses have determined that E. sakazakii comprises a number of genomospecies, and it has been prop...
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PMID: 18687498
PDF is available here.
PDF is available here.
Haemophilia (14)5 2008
Abstract
We studied 163 mild A haemophiliacs, and detected discrepancies in 20% of the patients, most of whom presented higher levels of FVIII:C with the one-stage assay. In nine families, the FVIII mutation was found, while three showed mutations not previously described (Leu1978Phe and Ser1791Pro associate...
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PMID: 18540892
PDF is available here.
PDF is available here.
Abstract
Crotalus durissus cumanensis is an endemic rattlesnake found in Venezuela and Colombia. In this study, a comparative analysis of hemorrhagic, coagulation and fibrino(geno)lytic activities in the presence or absence of protease inhibitors was performed with venoms of the same species Crotalus durissu...
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PMID: 18685436
PDF is available here.
PDF is available here.
Abstract
We conducted a large clinical study performing standard coagulation testing on more than 2,000 clinical samples randomly chosen from a high-volume laboratory in a tertiary care hospital. Results demonstrated that photo-optical clot detection and electro-mechanical detection systems were highly corre...
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PMID: 18685440
PDF is available here.
PDF is available here.
Abstract
The purpose of the present study was to compare the international normalized ratio with a chromogenic factor X (CFX) assay for monitoring patients on oral anticoagulant therapy using the DiaPharma CFX method on a STA-R Evolution coagulation analyzer. International normalized ratio values were correl...
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PMID: 18685434
PDF is available here.
PDF is available here.
Abstract
Novel indolo[3,2-b]carbazole derivatives and a chromogenic-sensing 5,12-dihydroindolo[3,2-b]carbazole have been synthesized starting from tetra-tert-butylated 6,12-diaryl-5,11-dihydroindolo[3,2-b]carbazoles, which were prepared via an efficient tert-butylation of 6,12-diaryl-5,11-dihydroindolo[3,2-b...
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PMID: 18600267
PDF is available here.
PDF is available here.
Abstract
We report a simple twisted intramolecular charge transfer (TICT) chromogenic chemosensor for rapid and selective detection of Hg(2+) and Cu(2+). The sensor was composed of an electron-acceptor 4-fluoro moiety and an electron-donor 7-mercapto-2,1,3-benzoxadiazole species where the S together with the...
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PMID: 18585232
PDF is available here.
PDF is available here.
Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.] (Chapter 10) 2008
Abstract
Immunoblotting (western blotting) is used to identify specific antigens recognized by polyclonal or monoclonal antibodies. This unit provides protocols for all steps, starting with solubilization of the protein samples, usually by means of SDS and reducing agents. Following solubilization, the mater...
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PMID: 18633991
PDF is available here.
PDF is available here.
Abstract
A chromogenic assay for the tissue factor-mediated thrombin generation was developed, the extrinsic coagulation activity assay: 50 microL citrated plasma is incubated with 5 microL tissue factor in 6% albumin and 250 mM CaCl2. After 1-minute (37 degrees C) coagulation reaction time, (extrinsic coagu...
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PMID: 18586682
PDF is available here.
PDF is available here.
Haemophilia (14 Suppl 3)s3 2008
Abstract
The correct diagnosis of factor VIII deficiency and the assessment of severity of the disease are essential for a patient-tailored treatment strategy. An optimal diagnostic procedure comprises sensitive and specific screening methods and factor VIII activity assays. Different screening reagents show...
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PMID: 18510526
PDF is available here.
PDF is available here.
Inorganic Chemistry (47)12 2008
Abstract
Emissive europium hydroxide nanorods (ENR) (20 nm x 500 nm) functionalized by a surface coating of chromophore-containing organically modified silicate (ORMOSIL) layer, have been synthesized and characterized by high-resolution transmission electron microscopy (TEM). Low-temperature photophysical ch...
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PMID: 18491890
PDF is available here.
PDF is available here.