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Cross-Linking Reagents (0):
Recent article
Cross-Linking Reagents
Biomedical Materials (6)5 2011
Abstract
Gelatin-based nanofibrous scaffolds with a mean fiber diameter of 300 nm were prepared with and without micrometer-sized polyethylene glycol (PEG) fibers that served as sacrificial templates. Upon fabrication of the scaffolds via electrospinning, the gelatin fibers were crosslinked w...
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PMID: 21931195
PDF is available here.
PDF is available here.
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Abstract
Abstract
Recently, poly(ester urethanes) were investigated for use as ligament grafts due to their exceptional mechanical properties and highly tunable structure; however, these grafts are susceptible to hydrolytic degradation that occurs independent of tissue regeneration. To address this li...
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PMID: 21681959
PDF is available here.
PDF is available here.
Biomacromolecules (12)6 2011
Abstract
We have designed and produced a modular, recombinant resilin-like polypeptide bearing both mechanically active and biologically active domains to create novel biomaterial microenvironments for engineering mechanically active tissues such as blood vessels, cardiovascular tissues, and vocal folds. Pre...
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PMID: 21553895
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
We present a basic method for the purification of the endogenous Par-6/aPKC protein complex, which plays a central role in orchestrating asymmetric cell divisions in the developing nervous system of Drosophila. The procedure can be subdivided into the following steps: acquisition of sufficient start...
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PMID: 18641959
PDF is available here.
PDF is available here.
Abstract
Nano-/micrometer-scaled films and capsules made of low-fouling materials such as poly(ethylene glycol) (PEG) are of interest for drug delivery and tissue engineering applications. Herein, the assembly and degradation of low-fouling, alkyne-functionalized PEG (PEG(Alk) ) multilayer fi...
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PMID: 21425467
PDF is available here.
PDF is available here.
Abstract
The development of suitable biomimetic three-dimensional scaffolds is a fundamental requirement of tissue engineering. This paper presents the first successful attempt to obtain electrospun gelatin nanofibers cross-linked with a low toxicity agent, genipin, and able to retain the ori...
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PMID: 21095244
PDF is available here.
PDF is available here.
Abstract
We have previously shown that the foreign body reaction (FBR) against crosslinked collagen type I (Col-I) differs between subcutaneous and epicardial implantation sites; Col-I was quickly degraded epicardially, whereas degradation was attenuated subcutaneously. The current study set out to dissect t...
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PMID: 20661871
PDF is available here.
PDF is available here.
Abstract
We expose a known rubber material to controlled dosages of γ radiation and utilize a newly developed multiquantum nuclear-magnetic-resonance technique to characterize the MWD as a function of radiation. We show that such data along with mechanical stress-strain measurements are amenable to accurate...
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PMID: 21517517
PDF is available here.
PDF is available here.
Abstract
Poly(A) polymerase (PAP) synthesizes the polyadenine tail at the 3'-end of messenger RNA. A disulfide cross-linking strategy was implemented to obtain a complex between bovine PAP (bPAP) and a 15-mer oligo(A). All seven endogenous cysteines were mutated to eliminate nonspecific cross-linked complex...
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PMID: 21301096
PDF is available here.
PDF is available here.
Abstract
We describe an alternative, novel approach to measure receptor activation by detecting and quantifying ligand-receptor complexes in FFPE specimens. We used hepatocyte growth factor (HGF)-c-MET as our model ligand-receptor system. HGF is the only known ligand of the c-MET tyrosine kinase receptor and...
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PMID: 21283737
PDF is available here.
PDF is available here.
Abstract
We have developed a procedure for efficient recovery of otherwise labile protein-protein interactions. We have named the procedure "ReCLIP" (Reversible Cross-Link Immuno-Precipitation) to reflect the primary elements of the method. Using cell-permeable, thiol-cleavable crosslinkers, normally labile...
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PMID: 21283770
PDF is available here.
PDF is available here.
Abstract
The potential of chitosan, a fishery waste-based material, as a soil amendment to clean-up metal contaminated soil was investigated. Chitosan was treated using glutaraldehyde (GLA), epichlorohydrin (ECH) and ethylene glycol diglycidyl ether (EGDE) as cross-linking reagents to enhance its chemical st...
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PMID: 20832259
PDF is available here.
PDF is available here.
Abstract
We describe an in situ formed hydrogel based on a water-soluble derivative of chitosan, hydroxypropyl chitosan (HPCTS), and sodium alginate dialdehyde (SAD). Periodate oxidized alginate rapidly cross-links HPCTS due to Schiff's base formation between the available aldehyde and amino groups. Hydrogel...
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PMID: 20832263
PDF is available here.
PDF is available here.
Acta Biomaterialia (7)1 2011
Abstract
I collagen mineralization model. In the presence of a polyacrylic acid-containing mineralization medium optimal intrafibrillar mineralization of the EDC-cross-linked collagen was achieved using 500 and 1000 μg ml⁻¹ PVPA. The mineralized fibrils exhibited a hierarchical order of intrafibrillar mi...
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PMID: 20688200
PDF is available here.
PDF is available here.
Acta Biomaterialia (7)1 2011
Abstract
Biological scaffolds used in tissue engineering are incorporated in vivo by a process of cellular in-growth, followed by host-mediated degradation and replacement of these scaffolds, in which phagocytic cells from the monocyte/macrophage cell lineage play a key role. The chemical deg...
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PMID: 20709200
PDF is available here.
PDF is available here.
Genipin-crosslinked microcarriers mediating hepatocellular aggregates formation and functionalities.
Abstract
We culture human liver carcinoma cell line (HepG2) cells on genipin-crosslinked gelatin microspheres of two different sizes. Results suggest that both microcarriers support cell adhesion, proliferation, and spontaneous formation of hepatocellular aggregates, among which the spheres with bigger size...
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PMID: 21105169
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)53 2010
Abstract
We analyzed the role of Tom7 in assembly of β-barrel proteins, including Tom40, a central channel subunit of the TOM40 complex, and porin. Depletion of Tom7 decreased transient accumulation of Tom40 at the level of the TOB complex and retarded assembly of porin in vitro. On the other hand, overexpr...
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PMID: 21036907
PDF is available here.
PDF is available here.
Abstract
Our objective was to investigate the properties of a three-dimensional silk sericin and PVA scaffold with and without glycerin and genipin at various concentrations. SEM showed that adding glycerin into scaffold gave better uniformity and porosity. Smaller pore sizes and better uniformity were found...
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PMID: 20804781
PDF is available here.
PDF is available here.
Abstract
Our experience based on the above and other cases suggest that CXL could be an effective tool in battling difficult cases of infectious keratitis. This treatment could present many advantages but will need further investigation....
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PMID: 21102196
PDF is available here.
PDF is available here.
Acta Biomaterialia (6)12 2010
Abstract
We propose that these materials function by retarding cytokine diffusion, with the non-crosslinked polymers being capable of retarding the diffusion of cytokines in the extracellular matrix and preventing engagement with receptors. In contrast, crosslinked materials have long diffusion lengths into...
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PMID: 20601239
PDF is available here.
PDF is available here.
Acta Biomaterialia (6)12 2010
Abstract
These results suggest the presence of multiple mechanisms as causes for increasing hydrogel modulus with freeze-thaw cycling, including hydrogen bonding between amorphous and/or crystalline regions, and the formation of highly concentrated regions of mostly amorphous PVA chains. It is possible that...
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PMID: 20601243
PDF is available here.
PDF is available here.
Acta Biomaterialia (6)12 2010
Abstract
We performed serial MPM and mechanical tests on acellular and cellularized (normal human lung fibroblasts) collagen hydrogels, before and after glutaraldehyde crosslinking. Following gel contraction over 16 days, cellularized collagen gel content approached that of native connective tissues (∼200...
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PMID: 20620246
PDF is available here.
PDF is available here.
Abstract
These results suggested that genipin-crosslinked SF/HBC nanofibrous scaffolds might be potential candidates for wound dressing and tissue-engineering scaffolds.
© 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010....
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PMID: 20824649
PDF is available here.
PDF is available here.
Abstract
We explored a recent photochemical method to crosslink solubilized wool keratoses, with the aim of producing a mechanically favorable biomaterial. Wool proteins were isolated by oxidizing the disulfides and extracting the resulting soluble keratoses. The α- and γ-keratose fractions were analyzed b...
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PMID: 20845488
PDF is available here.
PDF is available here.
Abstract
We designed nontoxic acid-sensitive biomaterials based on silyl ether chemistry. A host of silyl ether cross-linkers were synthesized and molded into relevant medical devices, including Trojan horse particles, sutures, and stents. The resulting devices were engineered to degrade under acidic conditi...
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PMID: 21105720
PDF is available here.
PDF is available here.
Abstract
We demonstrate that an analog of doxorubicin (a representative example of an anticancer therapeutic) conjugated to human serum albumin (HSA, a representative example of a macromolecular carrier) via this NEBI crosslinker can internalize and localize into acidic lysosomes of ovarian cancer cells. Flu...
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PMID: 20820656
PDF is available here.
PDF is available here.
Abstract
We present structural characterization of oxidation intermediates for these demethylases. Using a chemical cross-linking strategy, complexes of AlkB-double stranded DNA (dsDNA) containing 1,N(6)-etheno adenine (εA), N(3)-methyl thymine (3-meT) and N(3)-methyl cytosine (3-meC) are stabilized and cry...
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PMID: 21068844
PDF is available here.
PDF is available here.
Chemical Communications (46)40 2010
Abstract
A cross-linked hemoglobin bis-tetramer with oxygenation properties appropriate for a red cell substitute is produced by CuAAC conjugation of a dialkyne and two equivalents of an azide of β,β-cross-linked hemoglobin. The latter is formed efficiently by the aminolysis of the 3,5-dibromosalicylate es...
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PMID: 20852763
PDF is available here.
PDF is available here.
Current Eye Research (35)11 2010
Abstract
Acta Biomaterialia (6)11 2010
Abstract
Hyperbranched poly(ester amide) polymer (Hybrane S1200, M(n) 1200 gmol(-1)) was functionalized with maleic anhydride (MA) and propylene sulfide, to obtain multifunctional cross-linkers with fumaric and thiol end groups, S1200MA and S1200SH, respectively. The degree of substitution (D...
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PMID: 20561601
PDF is available here.
PDF is available here.
Acta Biomaterialia (6)11 2010
Abstract
We evaluate the adhesive behaviour of skeletal muscle cells (C2C12 myoblasts) during the initial steps of spreading on layer-by-layer films of controlled stiffness made of poly(L-lysine) and hyaluronan as model biomaterial surfaces for muscle tissue engineering. We show that integrin clustering, int...
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PMID: 20601233
PDF is available here.
PDF is available here.
Abstract
A novel visible light-crosslinkable porcine gelatin was prepared for gelation and micropatterning. The preparation employed a photo-oxidation-induced crosslinking mechanism. First, furfuryl groups were incorporated into the gelatin. Second, the modified gelatin was mixed in water with Rose Bengal, w...
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PMID: 20580950
PDF is available here.
PDF is available here.
Abstract
We describe a new family of biodegradable hybrid hydrogels fabricated in aqueous solution via long wavelength UV photo-crosslinking using maleic chitosan and polyethylene glycol diacrylate (PEGDA) as precursors. The maleic chitosan precursor was prepared by a simple one-step chemical modification of...
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PMID: 20416406
PDF is available here.
PDF is available here.
YAKUGAKU ZASSHI (130)10 2010
Abstract
We have employed a combination of cysteine mutagenesis and chemical crosslinking using a photoactivatable sulfhydryl reagent, benzophenone-4-maleimide, to obtain a covalent complex between human galectin-1 and a model glycoprotein ligand, asialofetuin. We previously obtained a crosslinked product wh...
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PMID: 20930491
PDF is available here.
PDF is available here.
Abstract
To follow the stability of a simultaneously delivered therapy that corrects aberrations and stiffens the corneal collagen of eyes with progressive keratoconus.
Two patients with progressive keratoconus underwent partial treatment (70% cylinder and sphere up to 50-μm central depth) with topographic...
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PMID: 20954679
PDF is available here.
PDF is available here.
Abstract
Myocardial infarction (MI) results in the death of cardiomyocytes (CM), which causes scar formation and pathological remodeling of the heart. The delivery of healthy myocytes or bone marrow cells reduces pathological remodeling after MI, however, current cell injection methods have l...
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PMID: 20540095
PDF is available here.
PDF is available here.
Abstract
We demonstrate the advantage of acetic acid over 1,1,1,3,3,3 hexafluoroisopropanol (HFP) in preventing collagen denaturation. A novel photochemical crosslinking method using rose bengal as the photoinitiator is also introduced. Using circular dichorism analyses, we demonstrate the fraction of collag...
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PMID: 20607867
PDF is available here.
PDF is available here.
Abstract
I was incorporated on the surface. After the coating procedure, collagen was crosslinked using the N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) conjugation method. In this study, hydroxyapatite scaffolds with uncrosslinked and crosslinked Type I c...
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PMID: 20740596
PDF is available here.
PDF is available here.
Biomedical Materials (5)5 2010
Abstract
I (CS/COL-I) scaffolds for cartilage engineering. CS/COL-I scaffolds were fabricated through freeze-drying and cross-linked by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide. SDSCs were isolated from synovium and cultured onto CS/COL-I scaffolds, constructs of which were incubated in serum-free chond...
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PMID: 20826911
PDF is available here.
PDF is available here.
Mutagenesis (25)5 2010
Abstract
We show that cells with Nek1 knockdown (KD) through stable RNA interference present a delay in DNA repair when treated with methyl-methanesulfonate (MMS), hydrogen peroxide (H(2)O(2)) and cisplatin (CPT). In particular, interstrand cross links induced by CPT take much longer to be resolved in Nek1 K...
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PMID: 20501547
PDF is available here.
PDF is available here.
Abstract
Poly(L-lactic acid), PLLA, a synthetic biodegradable polyester, is widely accepted in tissue engineering. Hyaluronic acid (HA), a natural polymer, exhibits an excellent biocompatibility, influences cell signaling, proliferation, and differentiation. In this study, HA crosslinking was...
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PMID: 20336752
PDF is available here.
PDF is available here.
Abstract
The bis-THF cores of annonaceous acetogenins were synthesized using (3R,4R)-1,5-hexadiene-3,4-diol (1) as the sole source of carbon atoms. The methylene acetal function was applied as a new linker/tether to facilitate the ring-closing metathesis.
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PMID: 20593079
PDF is available here.
PDF is available here.
Chemical Communications (46)31 2010
Abstract
A crosslinker anchoring process was developed to synthesize Fe(3)O(4)/NaYF(4):Yb,Er hetero-nanoparticles (NPs) with dual properties of superparamagnetism and upconversion (UC) emission. After ligand ozonolysis treatment, these NPs could be well dispersed in water.
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PMID: 20585692
PDF is available here.
PDF is available here.
Abstract
We assembled and characterized a phi29 connector-scaffolding complex, which can be incorporated into procapsids during in vitro assembly. Native mass spectrometry revealed that the connector binds at most 12 scaffolding molecules, likely organized as six dimers. A data-driven docking model, using in...
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PMID: 20124351
PDF is available here.
PDF is available here.
Molecular Cell (39)2 2010
Abstract
We show that the FANCM/FAAP24 complex is specifically required for the recruitment of replication protein A (RPA) to ICL-stalled replication forks. ICL-induced RPA foci formation requires the DNA-binding activity of FAAP24 but not the DNA translocase activity of FANCM. Furthermore, FANCM/FAAP24-depe...
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PMID: 20670894
PDF is available here.
PDF is available here.
Molecular Membrane Biology (27)4-6 2010
Abstract
We have used a site-directed cross-linking approach to show that, in both bacterial and yeast V-type ATPases, the N-terminal alpha-helical segments of the G and E subunits are closely aligned over a distance of up to 40 A. Furthermore, cross-linking coupled to mass spectrometry shows that the C-term...
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PMID: 20446876
PDF is available here.
PDF is available here.
Abstract
To assess integration of a biosynthetic corneal implant in dogs.
Three normal adult laboratory Beagles underwent ophthalmic examinations, including slit-lamp biomicroscopy, indirect ophthalmoscopy, applanation tonometry, and Cochet-Bonnet aesthesiometry. Biosynthetic corneas fabricated from glutaral...
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PMID: 20539221
PDF is available here.
PDF is available here.
Abstract
Dialdehyde starch (DAS) was used as a novel coupling agent to prepare chitosan carrier to immobilize the xylanase from Aspergillus niger A-25. Compared with glutaraldehyde-cross-linked chitosan (CS-GA) and pure chitosan beads, the DAS-cross-linked chitosan (CS-DAS) beads exhibited the highest xylana...
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PMID: 19823778
PDF is available here.
PDF is available here.
Abstract
Thermoresponsive nanohydrogels cross-linked by gold nanoparticles (AuNPs) were prepared by 1,3-dipolar cycloaddition reactions and in situ reversible addition-fragmentation chain-transfer (RAFT) polymerization. In order to synthesize thermoresponsive nanohydrogels, AuNPs decorated wi...
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PMID: 20669920
PDF is available here.
PDF is available here.
Abstract
Our results demonstrate that when Balb/c 3T3 fibroblasts were presented with a choice, they either migrated preferentially toward the high-collagen-compliant (low Young's modulus) side of the interfacial region or remained on the high-collagen region, suggesting a more dominant role for chemical sti...
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PMID: 20735103
PDF is available here.
PDF is available here.
Abstract
Phenylpyridal- and phenyldipyridal-based scaffolds have been designed and synthesized as novel helical peptide mimetics. The synthesis required optimisation and selective alkylation in producing 2,6-functionalized 3-hydroxypyridine derivatives for a convergent scheme. The pyridine analogues were cou...
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PMID: 20564292
PDF is available here.
PDF is available here.
Abstract
Abstract
We found a genetic interaction between the FA gene FANCC and the nonhomologous end joining (NHEJ) factor Ku70. Disruption of both FANCC and Ku70 suppresses sensitivity to cross-linking agents, diminishes chromosome breaks, and reverses defective homologous recombination. Ku70 binds directly to free...
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PMID: 20538911
PDF is available here.
PDF is available here.
Abstract
Benzyl esters of propiolic and beta-keto acids undergo catalytic decarboxylative coupling when treated with appropriate palladium catalysts. Such decarboxylative couplings allow the benzylation of alkynes without the use of strong bases and/or organometallics. This allows the synthesis of sensitive b...
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PMID: 20565096
PDF is available here.
PDF is available here.
Abstract
Well-defined AB2 Y-shaped miktoarm star copolymers of PNIPAM-b-(PZLL)2 and PNIPAM-b-(PLL)2 were synthesized through the combination of atom transfer radical polymerization (ATRP), ring-opening polymerization (ROP), and click chemistry, where PNIPAM, PZLL, and PLL are poly(N-isopropylacrylamide), pol...
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PMID: 20536122
PDF is available here.
PDF is available here.
Abstract
We describe KIAA1018, an evolutionarily conserved protein that has an N-terminal ubiquitin-binding zinc finger (UBZ) and a C-terminal nuclease domain. KIAA1018 is a 5'-->3' exonuclease and a structure-specific endonuclease that preferentially incises 5' flaps. Like cells from FA patients, human cell...
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PMID: 20603016
PDF is available here.
PDF is available here.
Abstract
We describe a highly conserved protein, KIAA1018/MTMR15/FAN1, that interacts with, and is recruited to sites of DNA damage by, the monoubiquitinated form of FANCD2. FAN1 exhibits endonuclease activity toward 5' flaps and has 5' exonuclease activity, and these activities are mediated by an ancient VR...
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PMID: 20603015
PDF is available here.
PDF is available here.
Abstract
Two types of measurement are presented that relate molecular events to macroscopic behavior of F-actin networks. First, shear modulus is measured by oscillating an embedded microbead. Second, a microbead is translated at constant rate and transitions in the resisting force are observed. The loading...
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PMID: 20866660
PDF is available here.
PDF is available here.
Abstract
We have focused specifically on the S-phase pathway of ICL repair in mammalian cells, which appears to be the major mechanism by which these lesions are removed in cycling cells. The various stages and components of this pathway are discussed, and a putative molecular model is presented. In addition...
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PMID: 20658646
PDF is available here.
PDF is available here.