Abstract
This article outlines empirical procedures for the storage of pure proteins with preservation of high levels of biological activity. It describes simple and workable means of preventing microbial contamination and proteolytic degradation, and the use of various types of stabilising additives. It set...
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PMID: 20978966
PDF is available here.
Abstract
Our goal is to develop a glucagon formulation that is stable as a clear ungelled solution, free of fibrils at a pH of 7 for at least 7 days at 37 °C.
Experimental glucagon formulations were studied for stability at 25 and 37 °C. Chemical degradation was quantified by reverse phase...
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PMID: 21129327
PDF is available here.
Abstract
Antioxidant capacities of methanolic extract and fatty acid composition of three Centaurea species were investigated. The antioxidant capacity of the extracts was evaluated by different assays, including total phenolic content, phosphomolybdenum assay, free radical scavenging activity (DPPH assay),...
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PMID: 20600531
PDF is available here.
Abstract
Leaf of Salvia miltiorrhiza Bunge, the waste part during the root harvest, is rich in health-promoting phenolics and is a novel resource of natural antioxidants. The acetone and methanol extracts of leaves (AL and ML, respectively) of S. miltiorrhiza were evaluated by various in vitro antioxidant as...
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PMID: 20600528
PDF is available here.
Abstract
We study the relationship between the shape and form of bovine serum albumin (BSA) protein in different solutions and their ability to form electrospun nanofibers. Small-angle X-ray scattering (SAXS) of the BSA in a water environment, in a 2,2,2-trifluoroethanol (TFE) environment, and in a TFE/beta-...
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PMID: 20450931
PDF is available here.
Abstract
The existence of different redox pools within the humate was confirmed, with the quinoid-centered redox entities showing the fastest kinetics. The results pertained to all size and polarity fractions....
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PMID: 20354802
PDF is available here.
Abstract
We have shown that shrimp shell wastes can be utilized and it's effective in the production of enzymes, antioxidants, peptide and reducing sugar, facilitating its potential use in biological applications and functional foods....
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PMID: 19838810
PDF is available here.
Abstract
Platinum-group-metal (Ru, Os, Rh, Ir, Pd and Pt) nanoparticles are synthesized in an aqueous buffer solution of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (200 mM, pH 7.4) under hydrothermal conditions (180 degrees C). Monodispersed (monodispersity: 11-15%) metal nano...
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PMID: 20512785
PDF is available here.
Abstract
We propose the use of 2-picoline-borane as the reducing agent, as a non-toxic alternative to the extensively used, but toxic sodium cyanoborohydride. Using dextran oligosaccharides and plasma N-glycans, we demonstrate similar labeling efficacies for 2-picoline-borane and sodium cyanoborohydride. The...
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PMID: 20391534
PDF is available here.
Abstract
We propose the use of 2-picoline-borane as the reducing agent, as a non-toxic alternative to the extensively used, but toxic sodium cyanoborohydride. Using dextran oligosaccharides and plasma N-glycans, we demonstrate similar labeling efficacies for 2-picoline-borane and sodium cyanoborohydride. The...
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PMID: 20391534
PDF is available here.
Abstract
We propose the use of 2-picoline-borane as the reducing agent, as a non-toxic alternative to the extensively used, but toxic sodium cyanoborohydride. Using dextran oligosaccharides and plasma N-glycans, we demonstrate similar labeling efficacies for 2-picoline-borane and sodium cyanoborohydride. The...
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PMID: 20391534
PDF is available here.
Abstract
We propose the use of 2-picoline-borane as the reducing agent, as a non-toxic alternative to the extensively used, but toxic sodium cyanoborohydride. Using dextran oligosaccharides and plasma N-glycans, we demonstrate similar labeling efficacies for 2-picoline-borane and sodium cyanoborohydride. The...
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PMID: 20391534
PDF is available here.
Abstract
We propose the use of 2-picoline-borane as the reducing agent, as a non-toxic alternative to the extensively used, but toxic sodium cyanoborohydride. Using dextran oligosaccharides and plasma N-glycans, we demonstrate similar labeling efficacies for 2-picoline-borane and sodium cyanoborohydride. The...
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PMID: 20391534
PDF is available here.
Abstract
We propose the use of 2-picoline-borane as the reducing agent, as a non-toxic alternative to the extensively used, but toxic sodium cyanoborohydride. Using dextran oligosaccharides and plasma N-glycans, we demonstrate similar labeling efficacies for 2-picoline-borane and sodium cyanoborohydride. The...
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PMID: 20391534
PDF is available here.
Abstract
We propose the use of 2-picoline-borane as the reducing agent, as a non-toxic alternative to the extensively used, but toxic sodium cyanoborohydride. Using dextran oligosaccharides and plasma N-glycans, we demonstrate similar labeling efficacies for 2-picoline-borane and sodium cyanoborohydride. The...
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PMID: 20391534
PDF is available here.
Abstract
We propose the use of 2-picoline-borane as the reducing agent, as a non-toxic alternative to the extensively used, but toxic sodium cyanoborohydride. Using dextran oligosaccharides and plasma N-glycans, we demonstrate similar labeling efficacies for 2-picoline-borane and sodium cyanoborohydride. The...
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PMID: 20391534
PDF is available here.
Abstract
We propose the use of 2-picoline-borane as the reducing agent, as a non-toxic alternative to the extensively used, but toxic sodium cyanoborohydride. Using dextran oligosaccharides and plasma N-glycans, we demonstrate similar labeling efficacies for 2-picoline-borane and sodium cyanoborohydride. The...
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PMID: 20391534
PDF is available here.
Abstract
We propose the use of 2-picoline-borane as the reducing agent, as a non-toxic alternative to the extensively used, but toxic sodium cyanoborohydride. Using dextran oligosaccharides and plasma N-glycans, we demonstrate similar labeling efficacies for 2-picoline-borane and sodium cyanoborohydride. The...
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PMID: 20391534
PDF is available here.
Abstract
We propose the use of 2-picoline-borane as the reducing agent, as a non-toxic alternative to the extensively used, but toxic sodium cyanoborohydride. Using dextran oligosaccharides and plasma N-glycans, we demonstrate similar labeling efficacies for 2-picoline-borane and sodium cyanoborohydride. The...
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PMID: 20391534
PDF is available here.
Abstract
We propose the use of 2-picoline-borane as the reducing agent, as a non-toxic alternative to the extensively used, but toxic sodium cyanoborohydride. Using dextran oligosaccharides and plasma N-glycans, we demonstrate similar labeling efficacies for 2-picoline-borane and sodium cyanoborohydride. The...
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PMID: 20391534
PDF is available here.
Abstract
We propose the use of 2-picoline-borane as the reducing agent, as a non-toxic alternative to the extensively used, but toxic sodium cyanoborohydride. Using dextran oligosaccharides and plasma N-glycans, we demonstrate similar labeling efficacies for 2-picoline-borane and sodium cyanoborohydride. The...
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PMID: 20391534
PDF is available here.
Abstract
We studied the ability of cauda epididymal sperm to adhere to the oviductal epithelium and to be released by sulfated glycoconjugates and the disulfide-reductant penicillamine. Surface protein sulfhydryls in cauda epididymal sperm were analyzed in the initial suspension, in sperm bound to the in vit...
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PMID: 20129657
PDF is available here.
Abstract
As a primary aim, several approaches to photothermal (thermal-lens) and optoacoustic determination of exogenous pyrogens (lipopolysaccharides) based on photometric procedures for their determination at the microgram level and below are compared. The limits of detection of lipopolysaccharides by ther...
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PMID: 20188934
PDF is available here.
Abstract
1,4-Butanediol is able to deliver two equivalents of H(2) in hydrogen-transfer reactions to ketones, imines, and alkenes. Unlike simple alcohols, which establish equilibrium in the reduction of ketones, 1,4-butanediol acts essentially irreversibly owing to the formation of butyrolact...
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PMID: 20112336
PDF is available here.
Abstract
1,4-Butanediol is able to deliver two equivalents of H(2) in hydrogen-transfer reactions to ketones, imines, and alkenes. Unlike simple alcohols, which establish equilibrium in the reduction of ketones, 1,4-butanediol acts essentially irreversibly owing to the formation of butyrolact...
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PMID: 20112336
PDF is available here.
Abstract
We have found these CGE methods to be applicable across a platform of mAbs in preclinical and clinical development, with the majority of mAbs requiring no modification to the method conditions. This methodology has been ICH validated and transferred to several supporting organizations. The data pres...
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PMID: 20087870
PDF is available here.
Abstract
We have found these CGE methods to be applicable across a platform of mAbs in preclinical and clinical development, with the majority of mAbs requiring no modification to the method conditions. This methodology has been ICH validated and transferred to several supporting organizations. The data pres...
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PMID: 20087870
PDF is available here.
Abstract
We have found these CGE methods to be applicable across a platform of mAbs in preclinical and clinical development, with the majority of mAbs requiring no modification to the method conditions. This methodology has been ICH validated and transferred to several supporting organizations. The data pres...
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PMID: 20087870
PDF is available here.
Abstract
Human NFU (also known as HIRIP5) has been implicated in cellular iron-sulfur cluster biosynthesis. Bacterial and yeast forms are smaller than the human protein and are homologous to the C-terminal domain of human NFU. This C-terminal domain contains a pair of redox active cysteines and demonstrates...
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PMID: 19722697
PDF is available here.
Abstract
We used chemicals that can cleave phosphodiester bonds of RNA and assessed their effects on the infectious agent. Lithium aluminum hydride, a reducing agent that can induce reductive cleavage of oxidized molecules such as carbonyls, carboxyl acids, esters, and phosphodiester bonds, did not affect ce...
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PMID: 19606066
PDF is available here.
Abstract
We show enzyme characteristics of pathogenic streptococcal UGLs and structural determinants for the enzyme substrate specificity. The putative genes for UGL and phosphotransferase system for amino sugar, a component of glycosaminoglycans, are assembled into a cluster in the genome of pyogenic and he...
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PMID: 19416976
PDF is available here.
Abstract
In the presence of Al-pillared bentonite with good sorption capacity, nanoscale zero-valent iron supported on Al-pillared bentonite (NZVI/Al-PILC) was prepared with NaBH4 and FeSO4 aqueous solution. The structure of NZVI/Al-PILC was characterized by X-ray diffraction (XRD) and Brunauer-Emmett-Teller...
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PMID: 19545005
PDF is available here.
Abstract
The thermodynamic properties of structurally similar Mn(III) and Mn(IV) complexes have been reinvestigated to understand their reactivity with substrates having C-H bonds. The complexes have the general formula [MnH(3)buea(O)](n-), where [H(3)buea](3-) is the tripodal ligand, tris[(N'-tert-butylurea...
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PMID: 19196005
PDF is available here.
Abstract
Colloidal silver nanoparticles were synthesized in water-in-oil microemulsion using silver nitrate solubilized in the water core of a microemulsion as source of silver ions, hydrazine hydrate solubilized in the water core of another one as reducing agent, cyclohexane as the continuous phase, and sod...
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PMID: 19455825
PDF is available here.
Abstract
Alditol bearing chitosans have shown the ability to reduce silver ions in mild conditions and without addition of exogenous reducing agents. The ion reduction induces the formation of a lactone moiety on the polysaccharide (Fetizon reaction) without causing C-C bond cleavage on the p...
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PMID: 19140690
PDF is available here.
Abstract
Alditol bearing chitosans have shown the ability to reduce silver ions in mild conditions and without addition of exogenous reducing agents. The ion reduction induces the formation of a lactone moiety on the polysaccharide (Fetizon reaction) without causing C-C bond cleavage on the p...
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PMID: 19140690
PDF is available here.
Abstract
Alditol bearing chitosans have shown the ability to reduce silver ions in mild conditions and without addition of exogenous reducing agents. The ion reduction induces the formation of a lactone moiety on the polysaccharide (Fetizon reaction) without causing C-C bond cleavage on the p...
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PMID: 19140690
PDF is available here.
Abstract
Alditol bearing chitosans have shown the ability to reduce silver ions in mild conditions and without addition of exogenous reducing agents. The ion reduction induces the formation of a lactone moiety on the polysaccharide (Fetizon reaction) without causing C-C bond cleavage on the p...
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PMID: 19140690
PDF is available here.
Abstract
Alditol bearing chitosans have shown the ability to reduce silver ions in mild conditions and without addition of exogenous reducing agents. The ion reduction induces the formation of a lactone moiety on the polysaccharide (Fetizon reaction) without causing C-C bond cleavage on the p...
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PMID: 19140690
PDF is available here.
Abstract
Alditol bearing chitosans have shown the ability to reduce silver ions in mild conditions and without addition of exogenous reducing agents. The ion reduction induces the formation of a lactone moiety on the polysaccharide (Fetizon reaction) without causing C-C bond cleavage on the p...
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PMID: 19140690
PDF is available here.
Abstract
Alditol bearing chitosans have shown the ability to reduce silver ions in mild conditions and without addition of exogenous reducing agents. The ion reduction induces the formation of a lactone moiety on the polysaccharide (Fetizon reaction) without causing C-C bond cleavage on the p...
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PMID: 19140690
PDF is available here.
Abstract
Alditol bearing chitosans have shown the ability to reduce silver ions in mild conditions and without addition of exogenous reducing agents. The ion reduction induces the formation of a lactone moiety on the polysaccharide (Fetizon reaction) without causing C-C bond cleavage on the p...
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PMID: 19140690
PDF is available here.
Abstract
Alditol bearing chitosans have shown the ability to reduce silver ions in mild conditions and without addition of exogenous reducing agents. The ion reduction induces the formation of a lactone moiety on the polysaccharide (Fetizon reaction) without causing C-C bond cleavage on the p...
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PMID: 19140690
PDF is available here.
Abstract
Alditol bearing chitosans have shown the ability to reduce silver ions in mild conditions and without addition of exogenous reducing agents. The ion reduction induces the formation of a lactone moiety on the polysaccharide (Fetizon reaction) without causing C-C bond cleavage on the p...
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PMID: 19140690
PDF is available here.
Abstract
We investigated the effects of H(2)O(2) and chloramine T(Ch-T) agents known to oxidize both cysteine and methionine residues, and 5, 5'-dithio-bis (2-nitrobenzoic acid) (DTNB)--a cysteine-specific oxidant, on the intracellular calcium in hippocampal neurons. The results showed that these three oxida...
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PMID: 18581229
PDF is available here.
Abstract
Despite considerable progress in the field of metal nanoparticles synthesis, major challenges remain in many practical applications of nanoparticles which require their immobilization on solid substrates, presenting additional difficulty in separation and processing. Here, transparent nanoporous cel...
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PMID: 19053296
PDF is available here.
Abstract
The ferryl derivatives of hemoglobin are products of the reactions of oxy- and methemoglobin with hydrogen peroxide. Ferryl hemoglobins, either with or without a radical site on the protein moiety, are oxidizing species. Plant polyphenols, flavonoids, have been shown to act as antioxidants in vivo a...
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PMID: 19774231
PDF is available here.
Abstract
Oligonucleotide that can reversibly alter the duplex-forming ability in response to an external factor has potential to be applied to various genome technologies, e.g. antisense and gene diagnosis technology. A novel bridged nucleoside which is capable of sensing an oxidant and reduc...
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PMID: 19749283
PDF is available here.
Abstract
Previous studies have demonstrated that Shewanella decolorationis S12 can grow on the azo compound amaranth as the sole electron acceptor. Thus, to explore the mechanism of energy generation in this metabolism, membranous vesicles (MVs) were prepared and the mechanism of energy generation investigat...
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PMID: 19190406
PDF is available here.
Abstract
We report a novel strategy for the biological synthesis of anisotropic gold and quasi-spherical silver nanoparticles by using apiin as the reducing and stabilizing agent. The size and shape of the nanoparticles can be controlled by varying the ratio of metal salts to apiin compound in the reaction m...
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PMID: 18977643
PDF is available here.
Abstract
We report a method for purification of IDP tau in an atmosphere of inert argon, which eliminates the need for reducing agents. We have used this method for preparing several IDP tau isoforms and found it useful in the investigation of monomeric tau toxicity in rat cerebral neurons....
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PMID: 18834853
PDF is available here.
Abstract
A detailed study of a procedure for the selective reduction of Δ2-isoxazolines to the corresponding β-hydroxy ketones is reported. The use of iron and ammonium chloride as the reducing agent in the presence of water results in a facile and chemoselective protocol for the preparation of β-hydroxy k...
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PMID: 18939869
PDF is available here.
Abstract
We investigated the reduction of a series of NACs with a model quinone (anthrahydroquinone-2,6-disulfonate monophenolate; AHQDS-) and a Fe(II)-catechol complex (1:2 Fe(II)-tiron complex; Fe(II)L2(6-)) over the pH range from 3 to 12 and variable reductant concentrations. Apparent kinetic isotope effe...
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PMID: 19068817
PDF is available here.
Abstract
We aimed to determine whether mtMGST1 also undergoes activation and about its function. When rats were treated with galactosamine/lipopolysaccharide (GalN/LPS), mtMGST1 activity was significantly increased, and the increased activity was reduced by the disulfide reducing agent dithiothreitol. In mit...
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PMID: 18634816
PDF is available here.
Abstract
The results show that in general the index of refraction increases during the photopolymerization process in a time frame from 0 to 40s and then approaches a steady-state value, which depends on the original composition of the admixture. The detailed refractive index change depends on the volume fra...
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PMID: 18420264
PDF is available here.
Abstract
We report that oxidation of CLC-1 may be the culprit underlying the controversy. When patches were excised from mammalian cells, the sensitivity to ATP was lost quickly--within 2-3 min. This loss of ATP sensitivity could be prevented or reversed by reducing agents. On the other hand, CLC-1 expressed...
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PMID: 18824589
PDF is available here.
Abstract
We also provide evidence that the CH-T-evoked inhibition of LTP is mediated via activation of glycogen synthase kinase-3beta (GSK-3beta), whereas the DTT-evoked enhancement of LTP is mediated via inactivation of GSK-3beta. These findings will benefit the understanding of the redox contribution to th...
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PMID: 18620045
PDF is available here.