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Detergents (1):
Recent article
Detergents
Abstract
I and avoids use of proteases or detergents that degrade the ECM. Characterization of the decellularized muscles using hematoxylin and eosin staining along with DNA quantification suggested complete removal of DNA, whereas biochemical analyses indicated no loss of collagens and only a slight reducti...
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PMID: 20973753
PDF is available here.
PDF is available here.
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Bulletin for the history of chemistry / Division of the History ofChemistry of the American Chemical Society (36)1 2011
Abstract
Abstract
Immunohistochemical techniques detect antigens in tissue sections by means of immunological and chemical reactions. This technique is highly sensitive and specific and can detect a wide variety of antigens in multiple animal species. This chapter reviews common immunohistochemical methods used in th...
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PMID: 20972748
PDF is available here.
PDF is available here.
Abstract
Although membrane proteins account for 20-30% of the coding regions of all sequenced genomes and play crucial roles in many fundamental cell processes, there are relatively few membranes proteins with known 3D structure. This is likely due to technical challenges associated with membrane protein ext...
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PMID: 20978985
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)52 2010
Abstract
We characterize the conformational changes in BAK and MCL-1 using detergents to mimic the membrane environment and study their interaction by in vitro pulldown experiments, size exclusion chromatography, titration calorimetry, and NMR spectroscopy. The nonionic detergent IGEPAL has little impact on...
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PMID: 21036904
PDF is available here.
PDF is available here.
Abstract
We have characterized the activity of γ-secretase complexes under a variety of detergent solubilization and reconstitution conditions, and the structural state of proteoliposomes by electron microscopy. We found that γ-secretase activity is highly dependent on the physical state or integrity of th...
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PMID: 20937251
PDF is available here.
PDF is available here.
Biofouling (26)8 2010
Abstract
The goal of this comparative study was to investigate biofilm forming microorganisms living in washing machines (WMs). Biofilms were sampled from 11 washing machines from four countries and three continents. Among the 94 isolated strains, 30% were potential human pathogens. Representative strains we...
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PMID: 20954022
PDF is available here.
PDF is available here.
Abstract
I digestion of chromatin in cultured cells results in loss of nuclear Pkp-1, suggesting that Pkp-1 associates specifically with nuclear components. In addition, in vitro assays revealed that the amino-terminal head domains of Pkps-1 and -2 were sufficient to bind single-stranded DNA. Induction of DN...
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PMID: 20613778
PDF is available here.
PDF is available here.
Abstract
We have proposed a possibility of another important sphingolipid function associated with cold acclimation. In animal cells, it has been known that the plasma membrane contains microdomains due to the chanracteristics of sphingolipids and sterols, and the sphingolipid- and sterol-enriched microdomai...
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PMID: 20818179
PDF is available here.
PDF is available here.
Abstract
Abstract
We treated lungs from adult rats using a procedure that removes cellular components but leaves behind a scaffold of extracellular matrix that retains the hierarchical branching structures of airways and vasculature. We then used a bioreactor to culture pulmonary epithelium and vascular endothelium o...
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PMID: 20576850
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)29 2010
Abstract
We have previously shown that two recombinant SP-A2 mutant proteins (G231V and F198S) remain within the endoplasmic reticulum (ER) of A549 cells and are not secreted into the culture medium. The pathogenic mechanism of the mutant proteins is unknown. Here we analyze all common and rare variants of t...
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PMID: 20466729
PDF is available here.
PDF is available here.
Abstract
We report that AcbA is localized in vesicles that accumulate in the cortex of prespore cells just prior to sporulation. These vesicles are not observed after cells are stimulated to release AcbA but remain visible after stimulation in cells lacking the Golgi reassembly stacking protein (GRASP). Acyl...
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PMID: 20472692
PDF is available here.
PDF is available here.
Abstract
We have described a series of small-molecule inhibitors of dynamin [myristyl trimethyl ammonium bromides (MiTMAB)] that competitively interfere with the ability of dynamin to bind phospholipids and prevent receptor-mediated endocytosis. We now report that dynII functions specifically during the absc...
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PMID: 20571068
PDF is available here.
PDF is available here.
Abstract
We present a systematic approach to obtain homogeneous and mono-disperse zinc transporter ZntB in quantities sufficient for structural and biochemical studies. Important aspects of this study that can be applied to other membrane proteins are also discussed.
Copyright 2010 Elsevier Inc. All rights r...
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PMID: 20159043
PDF is available here.
PDF is available here.
Pediatric Research (68)1 2010
Abstract
We evaluated the efficacy of HPbetaCD in the feline model of NPC disease and recognized a dose-dependent increase in hearing threshold associated with therapy as determined by brain stem auditory evoked response (BAER) testing. To further assess the effect of HPbetaCD on hearing threshold, normal ca...
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PMID: 20357695
PDF is available here.
PDF is available here.
Abstract
We investigated whether accumulation of detergent-insoluble EAAT2 is related to cognitive impairment and neuropathologic changes in AD by quantifying detergent-insoluble EAAT2 levels in hippocampus and frontal cortex of cognitively normal patients, patients with clinical dementia rating of 0.5 (mild...
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PMID: 20535038
PDF is available here.
PDF is available here.
Abstract
We created a non-palmitoylated SARS-CoV S protein by mutating all nine cytoplasmic cysteine residues. Palmitoylation of SARS-CoV S was required for partitioning into detergent-resistant membranes and for cell-cell fusion. Surprisingly, however, palmitoylation of S was not required for interaction wi...
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PMID: 20580052
PDF is available here.
PDF is available here.
Abstract
We show that MPD-flippase activity can be reconstituted in large unilamellar proteoliposomes prepared from phosphatidylcholine and Triton X-100-solubilized rat liver ER-membrane proteins. Using carboxy-2,2,6,6-tetramethylpiperidine 1-oxyl NO(+) as a topological probe to selectively oxidize MPD molec...
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PMID: 20534553
PDF is available here.
PDF is available here.
Abstract
BACDeltaNC/Delta RS is a thermostable variant derived from the truncated alpha-amylase (BAC Delta NC) of alkaliphilic Bacillus sp. strain TS-23. With the aim of enhancing its resistance towards chemical oxidation, Met231 of BAC Delta NC/Delta RS was replaced by leucine to create BAC Delta NC/Delta R...
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PMID: 20471589
PDF is available here.
PDF is available here.
Abstract
H1N1 was completely inactivated to undetectable levels in 1 minute of 70% ethanol, 70% 1-propanol, and solvent/detergent treatments in the surface tests as well as in the suspension tests. H1N1 was completely inactivated in 1 minute of 0.1 mol/L NaOH treatment in the suspension tests and also effect...
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PMID: 20430477
PDF is available here.
PDF is available here.
Abstract
We describe a Fast Agrobacterium-mediated Seedling Transformation (FAST) technique for transient gene expression analysis in Arabidopsis and other dicot or monocot species. This technique makes use of the cocultivation of young plant seedlings with Agrobacterium in the presence of the surfactant Sil...
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PMID: 20439415
PDF is available here.
PDF is available here.
Abstract
The numbers of inflammatory and non-inflammatory lesions decreased on both sides. A rebound tendency was noted for cleanser A with respect to inflammatory lesions at 4 weeks post-discontinuation, whereas inflammatory lesions continued to decrease on sides treated with cleanser B during this period....
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PMID: 20394494
PDF is available here.
PDF is available here.
Abstract
We were able to choose the detergent composition in which the membrane-like structure of the VSD is preserved....
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PMID: 20356311
PDF is available here.
PDF is available here.
Abstract
In this work, a sandwich monoclonal-based ELISA for quantifying the HBsAg obtained from yeast cells was standardized and validated. The monoclonal antibody employed in this assay reacts uniformly with different molecular isoforms of r-HBsAg. Immunoassay allowed the r-HBsAg quantification in an analy...
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PMID: 20188925
PDF is available here.
PDF is available here.
Journal of Proteomics (73)6 2010
Abstract
We report on a study with the goal to find a standardized protocol based on the analysis of 18 human proteins localized in 11 different organelles and subcellular structures. Six fixation protocols were tested based on either dehydration by alcohols (methanol, ethanol or iso-propanol) or cross-linki...
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PMID: 19896565
PDF is available here.
PDF is available here.
Biophysical Journal (98)8 2010
Abstract
The effect of nonionic detergents of the n-alkyl-beta-D-glucopyranoside class on the ordering of lipid bilayers and the dynamics of membrane-embedded peptides were investigated with 2H- and 31P-NMR. 1,2-dipalmitoyl-sn-glycero-3-phosphocholine was selectively deuterated at methylene segments C-2, C-7...
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PMID: 20409472
PDF is available here.
PDF is available here.
Biophysical Journal (98)8 2010
LCP-Tm: an assay to measure and understand stability of membrane proteins in a membrane environment.
Abstract
We have developed a robust and accurate assay, LCP-Tm, for measuring the thermal stability of membrane proteins embedded in an LCP matrix. In its two implementations, protein denaturation is followed either by a change in the intrinsic protein fluorescence on ligand release, or by an increase in the...
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PMID: 20409473
PDF is available here.
PDF is available here.
Abstract
We demonstrate for the first time a detergent-aided method to produce polymersomes. Peptidic polymersomes were formed from the rod-rod block copolymer PBLG(36)-E, where PBLG is hydrophobic poly(gamma-benzyl l-glutamate) and E is a hydrophilic designed peptide. The block copolymer was first solubiliz...
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PMID: 20329745
PDF is available here.
PDF is available here.
Abstract
Although SDS-PAGE is the method of choice for most denaturing gel electrophoresis procedures, the anionic detergent SDS still presents some drawbacks. For example, SDS forms crystals at low temperatures and, in some cases, causes proteins to aggregate or precipitate. In addition, some proteins are n...
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PMID: 20360366
PDF is available here.
PDF is available here.
Abstract
We describe a novel utilization of serum that may have broad applications in tissue decellularization....
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PMID: 19419244
PDF is available here.
PDF is available here.
Abstract
Since 1998, the Aquitaine-Limousin branch of the French Blood Institute has set up a parvovirus B19 (PV B19) systematic screening on each unit of plasma to be treated by solvent-detergent procedure for virus inactivation. Parvovirus B19 nucleic acid systematic testing in plasma pools became mandator...
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PMID: 20674438
PDF is available here.
PDF is available here.
Abstract
We recently proposed that this is related to the fact that the N-terminal half of this protein (C82) is an intrinsically unstructured protein (IUP) domain. IUP domains can adopt a secondary structure when they are interacting with another molecule, such as a nucleic acid or a protein. It is also pos...
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PMID: 20453932
PDF is available here.
PDF is available here.
Journal of Membrane Biology (234)3 2010
Abstract
We report that DRMs can be obtained by treating human erythrocytes with the nonionic detergents Triton X-100 or octaethylene glycol monododecyl ether (C(12)E(8)) at 37 degrees C, and by treatment at 4 degrees C of cholesterol-depleted erythrocytes. Electron paramagnetic resonance with spin labels in...
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PMID: 20339840
PDF is available here.
PDF is available here.
Analytical Biochemistry (402)2 2010
Abstract
We describe a sandwich ELISA that allows for the quantification of a diverse array of synaptic membrane and scaffold proteins such as munc-13, gephyrin, the NMDA R1 receptor, synaptic vesicle membrane proteins and SNAREs. The assay is based on initial solubilization by the denaturing detergent SDS,...
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PMID: 20371221
PDF is available here.
PDF is available here.
Methods in Molecular Biology (607)607 2010
Abstract
We provide protocols for the cell-free expression of membrane proteins in different modes including their expression as precipitate as well as their direct synthesis into detergent micelles or lipid bilayers....
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PMID: 20204858
PDF is available here.
PDF is available here.
Methods in Molecular Biology (607)607 2010
Abstract
We describe a novel method for the production of functional membrane proteins based on a wheat germ cell-free translation system. Using this method, functional membrane proteins are successfully synthesized in the presence of liposomes and a detergent. In addition, the synthesized membrane proteins...
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PMID: 20204859
PDF is available here.
PDF is available here.
Audio, Transactions of the IRE Professional Group on (Chapter 16) 2010
Abstract
Detergents are required for the extraction of hydrophobic proteins and for the maintenance of their solubility in solution. However, the presence of detergents in the peptide samples severely suppresses ionization in mass spectrometry (MS) analysis and decreases chromatographic resolution in LC-MS....
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PMID: 20155730
PDF is available here.
PDF is available here.
Nature Neuroscience (13)2 2010
Abstract
We found just such an influence; if taste cortex was inactivated when an odor was first presented, later presentations were properly appreciated only if taste cortex was again inactivated....
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PMID: 20023656
PDF is available here.
PDF is available here.
Abstract
These results demonstrate the usefulness of alkaline detergents and proteases for the degradation or destabilization of PrPSc....
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PMID: 20043137
PDF is available here.
PDF is available here.
Abstract
Extractive microbial fermentation for production of lipase by Serratia marcescens ECU1010 has been carried out in cloud point system. The cloud point system is composed of mixture nonionic surfactants with a ratio of Triton X-114 to Triton X-45 4:1 in aqueous solution. The lipase pre...
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PMID: 19798498
PDF is available here.
PDF is available here.
Abstract
We test the suggestion that laundering is sufficient to kill all life stages and thus help prevent reinfestation and dispersion. We establish minimum temperatures for washing and minimum temperatures and times for tumble-drying, as well as testing cold soaking, dry cleaning, and freezing as alternat...
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PMID: 20214378
PDF is available here.
PDF is available here.
Abstract
In transnasal small-caliber EGD, oolong tea instead of water as a washing solution for endoscopic lens cleansing is useful to maintain good visibility.
(c) Georg Thieme Verlag KG Stuttgart . New York....
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PMID: 19967631
PDF is available here.
PDF is available here.
Biochemistry (New York) (75)2 2010
Abstract
The effect of fatty acids and mitochondria-targeted lipophilic cations (SkQ1, SkQ3, MitoQ, and C(12)TPP) on tightly-coupled mitochondria from yeasts Dipodascus (Endomyces) magnusii and Yarrowia lipolytica was investigated. Micromolar concentrations of saturated and unsaturated fatty acids were found...
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PMID: 20367600
PDF is available here.
PDF is available here.
Abstract
This article demonstrates how the intracellular compartmentalization of the S. cerevisiae host cell can be exploited to impart selectivity during the primary purification of lipid-envelope virus-like particles (VLPs). The hepatitis B surface antigen (HBsAg) was used as the VLP model in this study. E...
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PMID: 19856403
PDF is available here.
PDF is available here.
Abstract
Air conditioning systems represent one important source of microbial pollutants for indoor air. In the past few years, numerous strategies have been conceived to reduce the contamination of air conditioners, mainly in hospital settings. The biocidal detergent BATT2 represents a natural product obtai...
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PMID: 20939877
PDF is available here.
PDF is available here.
Abstract
The 2009 Viral Clearance Symposium (Indianapolis, IN, USA) was held to interactively discuss methods for virus removal and inactivation during biopharmaceutical manufacture. Its origin was the result of worldwide regulatory and industry recognition that challenges, gaps, and opportun...
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PMID: 21516942
PDF is available here.
PDF is available here.
Abstract
The aza-Michael addition of 1,2,3,6-tetrahydrophthalimide with symmetrical fumaric esters has been performed efficiently in a solvent-free system at 100 °C and using 1,4-diazabicyclo[2.2.2]octane (DABCO) as a base in the presence of tetrabutylammonium bromide (TBAB). The products were obtained in g...
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PMID: 20966877
PDF is available here.
PDF is available here.
Journal of Oleo Science (59)2 2010
Abstract
The removal of the carbon black (CB) particles from the polymer substrates was investigated in water/ethanol mixtures. As substrates, the films and the fibrous assemblies of polyethylene, nylon 6 and cellulose acetate were used. The ultrasonic wave was applied as a mechanical action for cleaning. Th...
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PMID: 20103984
PDF is available here.
PDF is available here.
Gastroenterology Nursing (33)1 2010
Abstract
Journal of WOCN (37)3 2010
Abstract
Both washing procedures resulted in a low level of residual bacteria on the skin. No-rinse cleansers can be safely used as an alternative to soap and water washing of fragile skin....
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PMID: 20386330
PDF is available here.
PDF is available here.
Human Antibodies (19)1 2010
Abstract
IVIG can be prepared from fractionation of normal human plasma and it is used as a therapeutic drug for treatment of various diseases. IVIG has been for some time the high-growth product within the plasma derived products, at both a global and a national country level. Fractionation was performed ac...
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PMID: 20555125
PDF is available here.
PDF is available here.
Molecular Vision (16) 2010
A serine-type protease activity of human lens βA3-crystallin is responsible for its autodegradation.
Abstract
The purpose of the study was to determine whether the autodegradation of human βA3-crystallin is due to its intrinsic protease activity.
Recombinant His-tagged human βA3-crystallin was expressed in E. coli and purified by a Ni+2-affinity column chromatographic method. To determine protease activit...
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PMID: 21139689
PDF is available here.
PDF is available here.
Mikrobiologiia (79)5 2010
Abstract
Bacillus licheniformis 3C5, isolated as mesophilic bacterium, exhibited tolerance towards a wide range of non-polar and polar organic solvents at 45 degrees C. It produced an extracellular organic solvent-stable protease with an apparent molecular mass of approximately 32 kDa. The inhibitory effect...
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PMID: 21090506
PDF is available here.
PDF is available here.
British Journal of Nursing (19)13 2010
Abstract
Skin care is a fundamental aspect of basic nursing care, with the outcome of these interventions often used to gauge the quality of the care provided. However, possibly as a result of the 'basic' nature of this aspect of care, little attention has been paid to developing a firm evidence base to guid...
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PMID: 20606609
PDF is available here.
PDF is available here.
Abstract
Biosurfactant or microbial surfactants produced by microbes are structurally diverse and heterogeneous groups of surface-active amphipathic molecules. They are capable of reducing surface and interfacial tension and have a wide range of industrial and environmental applications. The present chapter...
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PMID: 20545273
PDF is available here.
PDF is available here.
Lakartidningen (107)3 2010
Abstract
Abstract
We discuss whether the amines present in the surfactant or the agent itself might be the cause of the condition. Because of the extensive use of surfactants in several types of industries, it is reasonable to think that their possible relationship with OA may have relevant health implications....
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PMID: 19806278
PDF is available here.
PDF is available here.
Biochemical Journal (424)2 2009
Abstract
Since evidence first appeared for 'detergent-resistant membranes' in the early to mid-1990s, cell biologists from a wide spectrum of biological sciences have been intrigued by the functional relevance of this indication of membrane heterogeneity, commonly referred to as 'lipid rafts'. Model membrane...
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PMID: 19888914
PDF is available here.
PDF is available here.
Abstract
These results indicate that it may be a potential component to use as a detergent additive for cold washing and in environmental bioremediation in cold regions....
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PMID: 19940938
PDF is available here.
PDF is available here.