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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Immunoglobulin Variable Region (2):
Recent article
Immunoglobulin Variable Region
Xenobiotica (41)5 2011
Abstract
We describe in detail the PBMC-based assay, which is more laborious but in our opinion represents a closer approximation of the in vivo situation. As with all other in vitro assays the results of such measurements are only an indication of the antibody potency in vivo, and animal studies and ultimat...
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PMID: 19252841
PDF is available here.
PDF is available here.
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Xenobiotica (41)5 2011
Abstract
We describe a recombinant method for the construction and production of a novel IgG-like BsAb molecule, using the variable domains of two fully human antibodies as the building blocks....
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PMID: 19252849
PDF is available here.
PDF is available here.
Abstract
We have expanded upon previous methods to develop a flow cytometry-based method to efficiently clone monoclonal antibodies (mAbs) from humans with expansions of RF-like, immunoglobulin heavy chain variable region (IgVH) 1-69 gene segment-containing B cells. The cloned variable regions are expressed...
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PMID: 20875420
PDF is available here.
PDF is available here.
Anti-Cancer Drugs (21)10 2010
Abstract
We generated a humanized GC33 from complementarity-determining region grafting with the aid of both the hybrid variable region and two-step design methods. The humanized antibody bound to GPC3 specifically and induced antibody-dependent cellular cytotoxicity as effectively as a chimeric GC33 antibod...
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PMID: 20847643
PDF is available here.
PDF is available here.
Hematology (15)4 2010
Abstract
We quantitated Ang-2 mRNA in purified mononuclear cells of 33 untreated CLL patients and compared the transcript levels to traditional as well as modern prognostic factors in patients with CLL (clinical stage, disease course, IgVH mutation status, CD38, and ZAP-70 expression). Elevated Ang-2 mRNA co...
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PMID: 20670479
PDF is available here.
PDF is available here.
Haematologica (95)7 2010
Abstract
Abstract
We failed in our initial effort to purify a prematurely developed Camelid-like E-tagged short scFv-K2 antibody that contained a complete variable region of the heavy chain and partial region of the light chain by using an anti-E-tag affinity column. To expedite the purification of this altered but i...
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PMID: 20060473
PDF is available here.
PDF is available here.
Abstract
We report our work on expression and purification of a mouse-derived anti-CBL single chain Fv (scFv) antibody in Escherichia coli (E. coli). An expression plasmid pBV220-CBL was constructed and transformed into E. coli BL21 (DH3) strain cells. After induction by temperature, the 6x His-tagged anti-C...
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PMID: 20206697
PDF is available here.
PDF is available here.
Immunogenetics (62)7 2010
Abstract
We have mapped and annotated the variable region of the immunoglobulin heavy (IGH) gene locus of the Brown Norway (BN) rat (assembly V3.4; Rat Genomic Sequence Consortium). In addition to known variable region genes, we found 12 novel previously unidentified functional IGHV genes and 1 novel functio...
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PMID: 20442993
PDF is available here.
PDF is available here.
Molecular Immunology (47)11-12 2010
Abstract
Some IgM cattle antibodies are amongst the largest known to exist in jawed vertebrates where CDR3H size may extend up to 61 amino acids. To understand the origin of such an exceptionally long CDR3H, bovine D(H) gene locus was completely characterized from Holstein cattle that revealed the presence o...
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PMID: 20435350
PDF is available here.
PDF is available here.
Journal of Cell Biology (189)7 2010
Abstract
We show that a POLH(-/-)/POLN(-/-)/POLQ(-/-) triple mutant displays no Ig gene conversion and reduced Ig hypermutation. Together, these data define a role for Polnu and Pol in recombination and suggest that the DNA synthesis associated with Ig gene conversion is accounted for by three specialized DN...
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PMID: 20584917
PDF is available here.
PDF is available here.
Journal of Cell Biology (189)7 2010
Abstract
We show that a POLH(-/-)/POLN(-/-)/POLQ(-/-) triple mutant displays no Ig gene conversion and reduced Ig hypermutation. Together, these data define a role for Polnu and Pol in recombination and suggest that the DNA synthesis associated with Ig gene conversion is accounted for by three specialized DN...
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PMID: 20584917
PDF is available here.
PDF is available here.
Abstract
Our study revealed VH2 overexpression and unique rearrangement in Ig VH genes from peripheral blood of AS patients. This may imply aberrant immunoglobulin gene rearrangement in B cell occurs in Korean AS patients, which requires further investigation....
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PMID: 20177145
PDF is available here.
PDF is available here.
Abstract
We used mAb 2E2 amino acid sequence information to create a homology model for the 3-D structure of its Fv fragment. Subsequent computational docking studies revealed the intermolecular interactions potentially responsible for mAb 2E2's cocaine binding properties. The driving force of cocaine bindin...
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PMID: 20185210
PDF is available here.
PDF is available here.
Abstract
We previously generated an engineered DT40 line named DT40-SW, whose AID expression can be reversibly switched on or off, and developed an in vitro Ab generation system using DT40-SW cells. To efficiently create an Ab library with sufficient diversity, higher hypermutation frequency is advantageous....
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PMID: 20416279
PDF is available here.
PDF is available here.
Abstract
We present a novel non-FcRn-dependent approach to reduce the elimination of IgG antibodies. Pharmacokinetic studies conducted in normal mice of various humanized IgG4 antibodies, which had identical constant regions but different variable region sequences, revealed that an antibody with a lower isoe...
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PMID: 20159773
PDF is available here.
PDF is available here.
Abstract
Chronic lymphocytic leukemia (CLL) remains the most common adult leukemia. The recent progress on research of molecular and cellular genetics of CLL promotes the development of the diagnosis, treatment and prognosis for CLL patients. IGVH gene mutation status is the most important prognostic marker...
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PMID: 20544986
PDF is available here.
PDF is available here.
Molecular Immunology (47)9 2010
Abstract
We show for the first time ever how one mAb (mAb-5) has polyreactive (protease, DNase, and RNase) catalytic activities (catAbs). Amino acid sequence analysis of the encoded variable-genes showed that the light chains of the hybridomas expressed the germline family genes V kappa 1A, bb1.1 and V kappa...
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PMID: 20356628
PDF is available here.
PDF is available here.
Leukemia Research (34)5 2010
Abstract
We assessed the distribution of the four prognostic cytogenetic markers (deletion 11q23, 13q14, 17p13 and trisomy 12) and VH mutational status in 32 SLL and 119 CLL. Trisomy 12 was most frequent (36% vs 13%, p=0.014) and 13q14 deletion was less frequent (9% vs 44%, p=0.001) in SLL in comparison with...
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PMID: 19959229
PDF is available here.
PDF is available here.
International Immunology (22)4 2010
Abstract
We characterized IgD(+) tonsillar GC cells. GC dominated by IgD(+) B cells were present in 10 of 67 tonsils analyzed. Three GC were additionally positive for CD70. Detailed analysis of one such GC by microdissection and single-cell DNA PCR revealed IgD(+) GC B cells undergoing somatic hypermutation...
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PMID: 20139173
PDF is available here.
PDF is available here.
Abstract
We described a novel two-step amplification of V(kappa) and V(H) genes with newly designed primer sets. Initially, we amplified V(kappa) and V(H) genes from their signal sequences to the joining region to keep antibody diversity as large as possible. Thereafter, highly degenerated primers were used...
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PMID: 19554473
PDF is available here.
PDF is available here.
Methods in Molecular Biology (607)607 2010
Abstract
We have developed an efficient and highly controllable ribosome display system using the Protein synthesis Using Recombinant Elements (PURE) system. The mRNA-ribosome-polypeptide ternary complex is highly stable in the PURE system and then the selected mRNA can be easily recovered, because activitie...
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PMID: 20204860
PDF is available here.
PDF is available here.
Abstract
We describe an assay for the identification of the expressed V(H) segment and its mutation status in CLL. This test uses whole blood-derived RNA and PCR primers annealing to the leader regions and the joining region segments. This approach allows more accurate determination of the IgV(H) mutation st...
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PMID: 20110453
PDF is available here.
PDF is available here.
Abstract
We have designed and prepared single-chain antibody fragments (scFvs) derived from the PrP(Sc)-specific murine monoclonal antibody V5B2. Using a new expression vector pMD204, we produced scFvs in two opposing chain orientations in the periplasm of Escherichia coli. Both recombinant antibody fragment...
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PMID: 19597999
PDF is available here.
PDF is available here.
Abstract
A 1557 bp DNA encoding scFv (G)-ETA-GAL4 protein gene was cloned and successfully expressed in inclusion body with approximate molecular weight of 57.0 kDa, which could be recognized by anti-His mAb. The scFv(G)-ETA-GAL4 proteins were purified by Ni-NTA column , and after renatured with the yield of...
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PMID: 20387470
PDF is available here.
PDF is available here.
Cancer Biomarkers (6)1 2010
Abstract
We identified 30% as being the best-cut-off for 17p-, 11q- and 6q-. In univariate analysis 17p- was found to be a significant predictor of the event only for the whole population. Multivariate analysis including all biological parameters, identified 11q deletion as the only significant regressor....
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PMID: 20164537
PDF is available here.
PDF is available here.
Cancer Biomarkers (6)1 2010
Abstract
We identified 30% as being the best-cut-off for 17p-, 11q- and 6q-. In univariate analysis 17p- was found to be a significant predictor of the event only for the whole population. Multivariate analysis including all biological parameters, identified 11q deletion as the only significant regressor....
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PMID: 20164537
PDF is available here.
PDF is available here.
Cancer Biomarkers (6)1 2010
Abstract
We identified 30% as being the best-cut-off for 17p-, 11q- and 6q-. In univariate analysis 17p- was found to be a significant predictor of the event only for the whole population. Multivariate analysis including all biological parameters, identified 11q deletion as the only significant regressor....
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PMID: 20164537
PDF is available here.
PDF is available here.
Cancer Biomarkers (6)1 2010
Abstract
We identified 30% as being the best-cut-off for 17p-, 11q- and 6q-. In univariate analysis 17p- was found to be a significant predictor of the event only for the whole population. Multivariate analysis including all biological parameters, identified 11q deletion as the only significant regressor....
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PMID: 20164537
PDF is available here.
PDF is available here.
Cancer Biomarkers (6)1 2010
Abstract
We identified 30% as being the best-cut-off for 17p-, 11q- and 6q-. In univariate analysis 17p- was found to be a significant predictor of the event only for the whole population. Multivariate analysis including all biological parameters, identified 11q deletion as the only significant regressor....
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PMID: 20164537
PDF is available here.
PDF is available here.
Cancer Biomarkers (6)1 2010
Abstract
We identified 30% as being the best-cut-off for 17p-, 11q- and 6q-. In univariate analysis 17p- was found to be a significant predictor of the event only for the whole population. Multivariate analysis including all biological parameters, identified 11q deletion as the only significant regressor....
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PMID: 20164537
PDF is available here.
PDF is available here.
Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders.
Abstract
We describe the results of a multiparametric analysis of the innate and early adaptive immune response after ex vivo stimulation of human immune cells with the VK3-20 protein. This objective has been pursued by implementing high-throughput technologies such as multiparameter flow cytometry and multi...
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PMID: 20170491
PDF is available here.
PDF is available here.
Italian journal of anatomy and embryology = Archivio italiano di anatomia ed embriologia (115)1-2 2010
Abstract
The past 15 years have witnessed an enormous effort in studying B-cell Chronic Lymphocytic Leukemia. A great number of researches brought significant novel information and a better understanding of the natural history of this disease. This mini review will focus on the studies related to the Immunog...
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PMID: 21072994
PDF is available here.
PDF is available here.
Abstract
We used flow cytometry analysis and sorting to determine immunophenotypic characteristics, clonality and molecular features of MBL from familial CLL kindreds. Single-cell analysis indicated four of six low-count MBL consisted of two or more unrelated clones; the other two MBL were monoclonal. 87% of...
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PMID: 19946263
PDF is available here.
PDF is available here.
Abstract
Telomere length was evaluated by terminal repeat fragment method in 66 previously untreated patients with B-chronic lymphocytic leukemia (B-CLL) to ascertain whether telomere shortening was associated with genomic aberrations, immunoglobulin variable heavy chain (IgVH) mutational status, CD38 and ZA...
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PMID: 20353271
PDF is available here.
PDF is available here.
Abstract
We previously reported that some of the rare broadly reactive, HIV-1 neutralizing antibodies are polyreactive, leading to the hypothesis that induction of these types of neutralizing antibody may be limited by immunologic tolerance. However, the notion that such antibodies are sufficiently autoreact...
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PMID: 20018688
PDF is available here.
PDF is available here.
Abstract
We describe the application of recombinant DNA and phage-display technologies for the construction of a phage-displayed human VH library, the panning of the library against a protein, and the expression, purification, and characterization of non-aggregating VHs isolated by panning....
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PMID: 19252860
PDF is available here.
PDF is available here.
Abstract
The rabbit antibody repertoire is an exceptional source for both polyclonal antibodies (pAbs) and monoclonal antibodies (mAbs) that combine high specificity with high avidity and affinity, respectively. In contrast to rabbit pAbs, which have been utilized for decades, rabbit mAbs are defined reagent...
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PMID: 19252834
PDF is available here.
PDF is available here.
Abstract
We have shown that mammalian cells can be used to display single-chain antibody fragments (scFvs) for affinity maturation. Using mammalian cell display one can isolate and engineer scFvs, Fabs, or whole IgGs for increased affinity and other specific biological functions. Here, we describe a mammalia...
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PMID: 19252852
PDF is available here.
PDF is available here.
Abstract
We have developed a general platform for engineering stability into antibody fragments. By promoting Escherichia coli cultures to secrete scFvs directly into growth media, automated screening methods can be applied to empirically evaluate multiple stability design strategies including rational, sequ...
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PMID: 19252856
PDF is available here.
PDF is available here.
Abstract
We describe a method for high-level production and purification of a fully functional immunoRNase fusion protein from supernatants of stably transfected mammalian cells....
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PMID: 19252845
PDF is available here.
PDF is available here.
Molecular Therapy (17)12 2009
Abstract
We hypothesized that targeting an antibody-based therapeutic to specifically abrogate intracellular A beta accumulation could prevent or slow disease onset. A beta 42-specific intracellular antibodies (intrabodies) with and without an intracellular trafficking signal were engineered from a previousl...
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PMID: 19638957
PDF is available here.
PDF is available here.
New Biotechnology (26)6 2009
Abstract
We systematically analyzed different multimerization domains in respect to three key parameters, crucial for the high-throughput generation of binders. (i) The compatibility to be displayed on phage (assessed for at least three different antibody formats, scFv, Fab and scFab) in combination with fiv...
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PMID: 19631299
PDF is available here.
PDF is available here.
médecine/sciences (25)12 2009
Abstract
The properties of monoclonal antibodies explain why they are such a successful class of therapeutic molecules. However, pionneered initial antibodies were of murine origin and triggered an immune response which limited the therapeutic potential of the antibody and generated deleterious effects. Cons...
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PMID: 20035696
PDF is available here.
PDF is available here.
Leukemia & Lymphoma (50)12 2009
Abstract
Our results have demonstrated significant up-regulation of WNT-3, WNT-4, WNT-5B, WNT-7B, WNT-9A, WNT-10A, and WNT-16B in patients with CLL compared to normal subjects (p < 0.05 to p < 0.0001). WNT gene expression analysis in different CLL subtypes showed a similar pattern of expression in progressiv...
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PMID: 19863181
PDF is available here.
PDF is available here.
Leukemia & Lymphoma (50)11 2009
Abstract
Analytical Biochemistry (395)1 2009
Abstract
We believe that the effectiveness of the competitive panning elution method and antigen-specific recombinant scFv antibodies obtained in this study are excellent candidates for antimycotic drugs....
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PMID: 19665444
PDF is available here.
PDF is available here.
Yonsei Medical Journal (50)4 2009
Abstract
In the peripheral blood of acute phase patients, T cells were significantly decreased (p < 0.05), whereas B cells were significantly increased (p < 0.05). When the first PCR was done on the B cell chains, V(H)1 to V(H)6 were all found to be expressed. The number of micro gene clones obtained from 3...
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PMID: 19718396
PDF is available here.
PDF is available here.
Molecular Biotechnology (43)1 2009
Abstract
Phage display technology has been widely used to isolate antibodies with specific properties. The objective of this study was to isolate anti-rhTNF-alpha scFvs from phage display library. However, the inserted genes of eluted phages were either incorrect or truncated. In order to address this issue,...
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PMID: 19412671
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)33 2009
Abstract
We formed disulfide bonds between Cys residues whose location in a peptide template representing the entire V3(CONSENSUS) epitope recognized by the broadly neutralizing 447-52D antibody was changed systematically. In a previous study [Mor, A., et al. (2009) Biochemistry 48, 3288-3303] we showed that...
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PMID: 19552398
PDF is available here.
PDF is available here.
Abstract
The overall results further demonstrate the potential utility of these two prosthetic groups for the radiohalogenation of internalizing monoclonal antibodies and their fragments. Specifically, the trastuzumab-derived double mutant fragment in combination with these residualizing agents warrants furt...
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PMID: 19647173
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (284)31 2009
Abstract
We applied a novel approach to select monoclonal phosphospecific antibodies directly from the full-length protein and without up-front phosphoamino acid identification. We chose as antigen GRASP65, a well studied Golgi phosphoprotein. Bacterially produced full-length protein was first incubated with...
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PMID: 19473967
PDF is available here.
PDF is available here.
Abstract
We found, however, that addition of the highly basic NLS severely compromised expression in the host-vector system used for the parental scFv. After testing a variety of host strains, fusion partners, and NLS sequences and placements, successful expression was obtained with a construct containing a...
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PMID: 19281848
PDF is available here.
PDF is available here.
Abstract
We examined the expression of AID and GANP in clinical CCA specimens to obtain information whether their expression levels are associated with the malignant grade of CCA. AID expression was similarly detected in the clinical cases of both well-differentiated and poorly-differentiated CCAs. On the co...
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PMID: 19578742
PDF is available here.
PDF is available here.
Biotechnology Letters (31)7 2009
Abstract
Abstract
scFv-H4 could be expressed in both BL21(DE3) and Origami (DE3). Compared with BL21(DE3), Origami(DE3) could express multifunctional scFv-H4 (35 mg/mL) and less in inclusion body (11% of the total expression). The expression of scFv-H4 was significantly affected by induction temperature rather than I...
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PMID: 19873751
PDF is available here.
PDF is available here.
Abstract
ErbB2 is a transmembrane tyrosine kinase, the overexpression of which causes abnormality and disorder in cell signalling and leads to cell transformation. Previously, an anti-ErbB2 single-chain chimeric antibody chA21 that specifically inhibits the growth of ErbB2-overexpressing cancer cells in vitro...
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PMID: 19574641
PDF is available here.
PDF is available here.
Abstract
These studies using phage display provide an alternative and viable method to screen for antibodies quickly and results show that good affinity antibodies against Ku86 have been successfully isolated and they can be used for further studies on MM and form the basis for further development as anti-ca...
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PMID: 19652854
PDF is available here.
PDF is available here.
Molecular Immunology (46)11-12 2009
Abstract
Classically, recombination between immunoglobulin gene segments uses a pair of recombination signal sequences (RSSs) with dissimilar spacers (the "12/23 rule"). Using a series of different genotyping assays, four different kinds of atypical rearrangements were identified at the murin...
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PMID: 19467709
PDF is available here.
PDF is available here.
Molecular Immunology (46)11-12 2009
Abstract
Classically, recombination between immunoglobulin gene segments uses a pair of recombination signal sequences (RSSs) with dissimilar spacers (the "12/23 rule"). Using a series of different genotyping assays, four different kinds of atypical rearrangements were identified at the murin...
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PMID: 19467709
PDF is available here.
PDF is available here.