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Peptide Library (0):
Recent article
Peptide Library
Abstract
We used an antagonistic VEGF ligand as a molecular scaffold to engineer dual-specific proteins that bound to VEGFR2 and α(v)β(3) integrin with antibody-like affinities and inhibited angiogenic processes in vitro and in vivo. Mutations were introduced into a single-chain VEGF (scVEGF) ligand that r...
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PMID: 21825147
PDF is available here.
PDF is available here.
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Journal of Proteomics (74)8 2011
Abstract
Spontaneous isoaspartyl formation from aspartyl dehydration or asparaginyl deamidation is a major source of modifications in protein structures. In cells, these conformational changes could be reverted by the protein L-isoaspartyl methyltransferase (PIMT) repair enzyme that converts...
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PMID: 21586350
PDF is available here.
PDF is available here.
Abstract
The proteome of untreated white wines (a Recioto made with Garganega grapes from the Veneto region) was explored in depth via capture with combinatorial peptide ligand libraries (CPLL) at four different pH values: pH 2.2, 3.8, 7.2, and 9.3. The combined data on the discoveries in the...
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PMID: 21702502
PDF is available here.
PDF is available here.
New Microbiologica (34)3 2011
Abstract
We successfully generated a mouse monoclonal antibody fragment from a phage display library directed against protein E7 of HPV18 avoiding antigen purification as for immunizing mice as for antibody library selection. Our work demonstrates the feasibility of phage antibody selections on antigens tran...
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PMID: 21811748
PDF is available here.
PDF is available here.
Abstract
Our results make the case that these two strategies greatly enhance the sampling of the conformational space near the native state. A detailed comparative analysis shows that our approach performs as well as state-of-the-art ab initio structure prediction protocols....
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PMID: 21714131
PDF is available here.
PDF is available here.
Abstract
The "invisible" proteome of a Cola drink, stated to be produced with a kola nut extract, has been investigated via capture with combinatorial peptide ligand libraries (CPLL). Indeed, a few proteins in the M(r) 15-20 kDa range could be identified by treating large beverage volumes (1...
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PMID: 21452894
PDF is available here.
PDF is available here.
Abstract
We describe the generation of a panel of 38 nanobodies, small HER2-binding fragments that are derived from heavy-chain-only antibodies raised in an immunized dromedary. In search of a lead compound, a subset of nanobodies was biochemically characterized in depth and preclinically tested for use as t...
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PMID: 21478264
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
We describe a targeted approach for affinity improvement of therapeutic antibodies. Sets of CDR residues that are solvent accessible and relatively diverse in natural antibodies are targeted for diversification. Degenerate oligonucleotides are used to generate combinatorial phage-displayed antibody...
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PMID: 19252851
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
The discovery of novel target antigens for antibody-based immunotherapy is still a major challenge. Antibody phage display is one of the technologies that is widely applied for the identification of novel cell surface molecules on intact eukaryotic cells and many reports describe the isolation of ph...
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PMID: 19153691
PDF is available here.
PDF is available here.
Abstract
B-cell receptors (BCRs) and their recognition of specific epitopes may play a pivotal role in the development and progression of chronic lymphocytic leukemia (CLL). In this study, the authors set up a model system to explore epitope reactivity and its clinical relevance in CLL.
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PMID: 21509766
PDF is available here.
PDF is available here.
Journal of Proteomics (74)4 2011
Abstract
We use a large, plasma based, proteome-derived peptide library as substrates along with mass spectrometry to explore the peptide bond specificity of three PI SVMPs and one PIII SVMP to determine their individual peptide cleavage consensus sequences. All of the proteinases assayed displayed a clear p...
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PMID: 21156218
PDF is available here.
PDF is available here.
Abstract
We report several proteins and peptides that exhibit heightened degradation sensitivity, for instance superoxide dismutase in liver, and peptidyl-prolyl cis-trans isomerase and insulin C-peptides in pancreas. Tissue sampling based on snap freezing produces a greater amount of degradation products an...
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PMID: 20110281
PDF is available here.
PDF is available here.
Abstract
To find specific polypeptides that bind porcine reproductive and respiratory syndrome virus (PRRSV) with high affinity and inhibit replication of PRRSV, we screened ligands on intact PRRSV virion by phage display library.
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PMID: 21465799
PDF is available here.
PDF is available here.
Biopolymers (95)5 2011
Abstract
Apical membrane antigen 1 (AMA1) is essential for malaria parasite invasion of erythrocytes and is therefore an attractive target for drug development. Peptides that bind AMA1 have been identified from random peptide libraries expressed on the surface of phage. Of these, R1, which binds to a hydropho...
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PMID: 21213258
PDF is available here.
PDF is available here.
Abstract
We have developed optimized methods that allow the rapid assembly of scFv libraries from chickens immunized with pools of immunogens. These methods allow the simplified generation of a single moderately sized library of single-chain Fv (scFv) and the subsequent isolation of diverse, high-affinity, a...
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PMID: 20978977
PDF is available here.
PDF is available here.
Abstract
We discuss the inherent limitations of classical polyclonal and monoclonal antibody generation and highlight an attractive alternative: generating high specificity, high affinity recombinant antibodies from alternative immune sources such as chickens, via phage display....
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PMID: 20978962
PDF is available here.
PDF is available here.
Abstract
We show that the peptides bind on the surface of these cells and obtained clones that could even specifically bind to only specific regions of cells indicating restricted distribution of the epitopes. What was peculiar and interesting was that the epitopes were carbohydrate in nature. This gives ins...
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PMID: 21364990
PDF is available here.
PDF is available here.
Abstract
The 21st Annual Antibody Engineering and 8th Annual Antibody Therapeutics international conferences, and the 2010 Annual Meeting of The Antibody Society, organized by IBC Life Sciences with contributions from The Antibody Society and two Scientific Advisory Boards, were held December...
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PMID: 21304271
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)45 2010
Abstract
We searched for novel ECE-specific peptide substrates. To this end, peptides derived from the big endothelin intermediate were tested using ECE-1 and ECE-2, leading to the identification of an ECE-1-specific substrate. Moreover, screening of our proprietary fluorigenic peptide Fluofast® libraries u...
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PMID: 20807771
PDF is available here.
PDF is available here.
Journal of Proteomics (73)12 2010
Abstract
We find that the only fining agent used is bovine casein, just like in white wines. Although the typical levels of casein found range between 45 to 85μg/L, in one case as little as 3.8μg/L of casein could be detected, an extremely high level of sensitivity, close to our lower detection limit of 1...
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PMID: 20813213
PDF is available here.
PDF is available here.
Abstract
We previously obtained a strong LPS-binding peptide, Li5-001, using the phage display method (Matsumoto et al., 2010. J. Microbiol. Methods. 82, 54-58). We modified the sequence the amino acid sequence of this peptide (KNYSSSISSIHAC), by replacing and deleting amino acids to obtain higher LPS-bindin...
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PMID: 20816904
PDF is available here.
PDF is available here.
Nature Biotechnology (28)11 2010
Abstract
We apply the latter strategy to antibody discovery, using a library of ∼10,000 human germline antibody Fabs created by de novo DNA synthesis and automated protein expression and purification. In multiplexed screening assays, we obtained specific hits against seven of nine antigens. Using sequence-...
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PMID: 20972421
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (26)10 2010
Abstract
to isolate novel breast cancer antigen for developing cancer marker and vaccine, a phage display peptide library was screened with serum antibody from breast cancer patients.
a phage display peptide library was screened with serum IgG from six breast cancer patients, and then identified the positive...
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PMID: 20937226
PDF is available here.
PDF is available here.
New Microbiologica (33)4 2010
Abstract
We have shown that monoclonal antibodies targeting the ectodomain of the A/M2 proton channel have antiviral activity in vitro. In this study, we generated both monoclonal antibody and phage displayed peptide against the eight amino acids comprising the ectodomain of the BM2 proton channel and invest...
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PMID: 21213589
PDF is available here.
PDF is available here.
Anticancer Research (30)9 2010
Abstract
These results suggest a potential use of this antibody generating method by phage display and indicate the potential of Fc-fusion proteins as therapeutic candidates....
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PMID: 20944114
PDF is available here.
PDF is available here.
Abstract
The advancement of molecular imaging hardware and disease targets has driven the demand of novel probes for diagnosis of human diseases. The high-throughput property of phage-display techniques makes the fast developments of these probes possible. The antibody and peptide phage display libraries hav...
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PMID: 20497117
PDF is available here.
PDF is available here.
Abstract
We describe a 21-residue peptide tag (designated as TARGET tag) and its use for rapid stable cell line development and purification. The ability of the anti-tag antibody P20.1 to withstand repetitive regeneration cycles has enabled the development of a sensitive surface plasmon resonance-based scree...
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PMID: 20566373
PDF is available here.
PDF is available here.
Abstract
Combinatorial peptide ligand libraries (CPLLs) have been adopted for harvesting and identifying traces of casein (used as a fining agent) present in white wines. Although minute amounts (200 microL) of CPLL beads are added to the entire content of a wine bottle (750 mL), they are able to sequester w...
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PMID: 20510390
PDF is available here.
PDF is available here.
Abstract
Abstract
We have previously described methodology to screen T7-phage NSCLC-cDNA libraries for phage-expressed proteins recognized by NSCLC-associated antibodies, and developed a multiplex assay that has excellent ability to discriminate NSCLC from control samples. This follow-up report describes the developm...
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PMID: 20543565
PDF is available here.
PDF is available here.
Inflammation Research (59)8 2010
Abstract
Our data demonstrate that phage display technology is a powerful tool for the identification of bioactive peptides. The identified FQHPSFI peptide may be used for the modulation of LPS-stimulated MIP-2 production in AMs....
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PMID: 20186460
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)30 2010
Abstract
We used phage display to identify novel peptides that specifically modulate GlyR function. Peptide D12-116 markedly enhanced GlyR currents at low micromolar concentrations but had no effects on the closely related gamma-aminobutyric acid type A receptors. This approach can readily be adapted for use...
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PMID: 20501662
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)29 2010
Abstract
We recently developed activatable cell-penetrating peptides (ACPPs) that target contrast agents to in vivo sites of matrix metalloproteinase activity, such as tumors. Here we use parallel in vivo and in vitro selection with phage display to identify novel tumor-homing ACPPs with no bias for primary...
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PMID: 20460372
PDF is available here.
PDF is available here.
FASEB Journal (24)7 2010
Abstract
We selected 16-mer peptides that interact with Bcl-X(L) from random and degenerate peptide libraries using mRNA display. The selected peptides have sequence similarity with the Bcl-2 family BH3 domains, and one of them has higher affinity (IC(50)=0.9 microM) than Bak BH3 (IC(50)=11.8 microM) for Bcl...
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PMID: 20181936
PDF is available here.
PDF is available here.
Abstract
To obtain the soluble single-chain fragment V (ScFv) monoclonal antibodies (McAbs) against the SSA antigen epitopes.
Three octapeptides (60000 SSA antigen residues 482-493 termed as P1 epitope, residues 310-323 termed as P2 epitope and residues 230-241 termed as P3 epitope) were synthesized on the l...
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PMID: 20979776
PDF is available here.
PDF is available here.
Infection and Immunity (78)7 2010
Abstract
We investigated the binding of YadA to collagen Toolkits, which are libraries of triple-helical peptides spanning the sequences of type II and III human collagens. YadA bound to many of them, in particular to peptides rich in hydroxyproline but with few charged residues. We were able to block the bi...
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PMID: 20439473
PDF is available here.
PDF is available here.
Abstract
HLA-A 0201-restricted virus-specific CD8(+) CTL do not appear to control HIV effectively in vivo. To enhance the immunogenicity of a highly conserved subdominant epitope, TV9 (TLNAWVKVV, p24 Gag(19-27)), mimotopes were designed by screening a large combinatorial nonapeptide library w...
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PMID: 20432235
PDF is available here.
PDF is available here.
Abstract
We confirmed that the initial DDDAM regimen, which mimics one of the clinical schedules (DDDCM), is highly immunogenic in macaques. Furthermore, adjuvanted synthetic long peptides divided into sub-pools and delivered into anatomically separate sites induced T-cell responses that were markedly broade...
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PMID: 20468055
PDF is available here.
PDF is available here.
Biochemistry (Washington) (49)26 2010
Abstract
Our results show that although all three enzymes require small residues at the P1' position, they have differential tolerance for Val and Thr at this position. The catalytic activity of human MetAP2 toward Met-Val peptides is consistently 2 orders of magnitude higher than that of MetAP1, suggesting...
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PMID: 20521764
PDF is available here.
PDF is available here.
Biochemistry (Washington) (49)26 2010
Abstract
We describe comprehensive mutagenesis of the light chain complementarity determining regions (CDRs) of HIV-1 antibody D5 (which binds its target, "5-Helix", with a reported K(D) of 50 pM). Combinatorial scanning mutagenesis libraries were prepared in which CDR residues on the D5 light chain were var...
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PMID: 20518570
PDF is available here.
PDF is available here.
Abstract
We report a solid-phase synthetic route to large combinatorial variations of this fundamental scaffold, extending the variety of substituted biomimetic molecules available to successfully implement the Distributed Drug Discovery (D3) project. In a single solid-phase sequence, compatible with basic a...
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PMID: 20657403
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (28)3 2010
Abstract
To screen and analyze the peptides in 12 phage-display peptide library specifically binding to the schistosomulum tegument of Schistosoma japonicum.
A 12 phage-display peptide library was screened with the S. japonicum schistosomula as the target cells for biopanning by degrees, positive clones pick...
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PMID: 20806497
PDF is available here.
PDF is available here.
Veterinary Microbiology (143)1 2010
Abstract
We show that boIFN-alpha-specific antibodies bind to the 90kDa gB subunit and efficiently neutralize BHV-1/gB2FuIN-alpha infectivity. We also show that inactivated BHV-1/gB2FuIN-alpha virions induce an antiviral state in cells incubated with UV-inactivated particles. These results demonstrate that t...
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PMID: 20193990
PDF is available here.
PDF is available here.
Abstract
Abstract
Abstract
We describe the use of two landscape phage libraries to obtain phage ligands against PC3 prostate carcinoma cells. Following a very stringent selection scheme, we were able to identify three phage ligands, bearing the fusion peptides, DTDSHVNL, DTPYDLTG and DVVYALSDD that demonstrated specificity an...
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PMID: 20185523
PDF is available here.
PDF is available here.
Abstract
The present study was focused on identifying cancer cell-specific internalizing ligands using a new kind of phage display library in which the linear or cysteine-constrained random peptides were at amino-terminus fusion to catalytically active P99 beta-lactamase molecules. The size and quality of li...
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PMID: 20219829
PDF is available here.
PDF is available here.
Abstract
We present the results of combining design and selection to remodel a protein-peptide binding interface, using the peptide PTIEEVD and the TPR1 module interaction as our test case. We initially used the program Rosetta to interrogate possible TPR1 sequences compatible with binding the peptide PTIEEV...
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PMID: 20304973
PDF is available here.
PDF is available here.
Abstract
Protein-protein interactions are critical to cellular processes yet the ability to predict and rationally design interactions is limited because of incomplete knowledge of the principles governing these interactions. The beta-lactamase inhibitory protein (BLIP)/beta-lactamase interaction has become...
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PMID: 20308189
PDF is available here.
PDF is available here.
Journal of Neuroimmunology (223)1-2 2010
Abstract
We selected a novel set of human anti-amyloid-beta peptide 1-42 (Abeta1-42) recombinant monoclonal antibodies in a single chain fragment variable (scFv) and a single-domain (VH) format. We demonstrated that these antibody fragments recognize in a specific manner amyloid-beta deposits in APP/Tg mouse...
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PMID: 20451261
PDF is available here.
PDF is available here.
Analytical Biochemistry (401)1 2010
Abstract
We present a new application of the noncompetitive phage anti-immunocomplex assay (PHAIA) by converting an existing competitive assay to a versatile noncompetitive sandwich-type format using immunocomplex binding phage-borne peptides to detect the brominated flame retardant, brominated diphenyl ethe...
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PMID: 20152791
PDF is available here.
PDF is available here.
Abstract
We previously generated an engineered DT40 line named DT40-SW, whose AID expression can be reversibly switched on or off, and developed an in vitro Ab generation system using DT40-SW cells. To efficiently create an Ab library with sufficient diversity, higher hypermutation frequency is advantageous....
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PMID: 20416279
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (26)6 2010
Abstract
The 20 clones were identified to be specific reactivity with the mAb E(9);F(10);. Amino acid sequence analysis revealed seven different types of mimotope sequence, most of which contained a common motif DXLXPP(X presents random acid amino), X was random amino acid. The Phage No.2(phage 2), the clone...
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PMID: 20487642
PDF is available here.
PDF is available here.
Abstract
The latest advances in combinatorial peptide ligand libraries, with their unique performance in discovering low-abundance species in proteomes, are reviewed here. Explanations of mechanism, potential applications, capture of proteomes at different pH values to enhance the total catch and quantitativ...
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PMID: 20536309
PDF is available here.
PDF is available here.
Abstract
We summarize and discuss the current status of proteomic techniques to identify protease specificity, cleavage sites and natural substrates with a particular focus on the cytotoxic lymphocyte granule serine proteases granzymes....
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PMID: 20536307
PDF is available here.
PDF is available here.
Abstract
We report the development of target-specific binding proteins based on the kringle domain (KD) ( approximately 80 residues), a ubiquitous modular structural unit occurring across eukaryotic species. By exploiting the highly conserved backbone folding by core residues, but using extensive sequence va...
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PMID: 20460308
PDF is available here.
PDF is available here.
Journal of Molecular Biology (398)5 2010
Abstract
We used two complementary techniques--yeast surface display screening from combinatorial peptide libraries and SPOT peptide array analysis--to elucidate specificity determinants for binding to Bcl-x(L)versus Mcl-1, two prominent prosurvival proteins. We screened a randomized library and identified B...
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PMID: 20363230
PDF is available here.
PDF is available here.
Chemical Reviews (110)5 2010
Abstract
Journal of Proteomics (73)7 2010
Abstract
We have unambiguously detected several others like, for instance: heat shock protein (81 kDa), proteasome subunit (30 kDa), protease inhibitor (8 kDa), hevamine A (43 kDa) and glyceraldehyde-3-phosphate dehydrogenase (37 kDa). Gene Ontology analysis of analyzed fractions has shown that major functio...
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PMID: 20226888
PDF is available here.
PDF is available here.
Chinese Medical Journal (123)9 2010
Abstract
Thirty-three out of fifty-eight (56.9%) of the isolated monoclonal phages exhibited high binding activity by ELISA. Ten of fifteen obtained phage model peptides were similar to KGF or epidermal growth factor (EGF). MTT assay data showed that four (No. 1 - 4) of the ten phage model peptides could pro...
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PMID: 20529562
PDF is available here.
PDF is available here.