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Protein Sorting Signals (2):
Recent article
Protein Sorting Signals
Abstract
We analyzed P30 derivatives having epitope tags engineered at various locations to demonstrate that posttranslational processing occurred in P30. Several potential cleavage sites predicted in silico were examined, and a processing-defective mutant was created to explore P30 maturation further. Our r...
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PMID: 21821772
PDF is available here.
PDF is available here.
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Abstract
Penicillin-binding proteins catalyze the biosynthesis of the peptidoglycan chains of the bacterial cell wall, which protects cells from osmotic pressure. Although Lmo0540 has been identified as a putative penicillin-binding protein that contributes to the virulence of Listeria monocytogenes, the bio...
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PMID: 21301102
PDF is available here.
PDF is available here.
Abstract
Glucoamylase from Aspergillus niger is an industrially important biocatalyst that is utilized in the mass production of glucose from raw starch or soluble oligosaccharides. The G1 isoform consists of a catalytic domain and a starch-binding domain connected by a heavily glycosylated linker region. Th...
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PMID: 21301084
PDF is available here.
PDF is available here.
Abstract
Vibrio hollisae, a halophilic species recently reclassified as Grimontia hollisae, is a causative agent of gastroenteritis and septicaemia. One important pathogenic Vibrio factor, thermostable direct haemolysin (TDH), has been purified and crystallized in two crystal forms using the vapour-diffusion...
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PMID: 21301091
PDF is available here.
PDF is available here.
Abstract
We have used an evolutionary approach to study DIA1 and DIA1R. We found DIA1 conserved from cnidarians to humans, indicating DIA1 evolution coincided with the development of the first primitive synapses. Nematodes lack a DIA1 homologue, indicating Caenorhabditis elegans is not suitable for studying...
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PMID: 21283809
PDF is available here.
PDF is available here.
Abstract
We hypothesized that Saccharomyces cerevisiae might represent a useful model system for studying torsinA function and the effects of its mutants. Since torsinA is proposed to function in protein homeostasis, we tested cells for their ability to respond to various stressors, using a fluorescent repor...
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PMID: 21818381
PDF is available here.
PDF is available here.
Abstract
We identify BORIS in the cytoplasm and nucleus of a wide range of normal and cancer cells. We compare the localization of CTCF and BORIS in the nucleus and demonstrate enrichment of BORIS within the nucleolus, inside the nucleolin core structure and adjacent to fibrillarin in the dense fibrillar com...
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PMID: 21811597
PDF is available here.
PDF is available here.
Abstract
I will review our current knowledge of the secretion of classical monomeric autotransporters and the methods that have been used to assess their folding during the translocation, both in vitro and in vivo....
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PMID: 21557061
PDF is available here.
PDF is available here.
Abstract
We expressed, purified, and characterized the recombinant protein containing immunomimetic peptide of benzo[a]pyrene....
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PMID: 21789801
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)3 2011
Abstract
The twin-arginine translocation (Tat) system operates in plant thylakoid membranes and the plasma membranes of most free-living bacteria. In bacteria, it is responsible for the export of a number of proteins to the periplasm, outer membrane or growth medium, selecting substrates by virtue of cleavabl...
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PMID: 21126506
PDF is available here.
PDF is available here.
Clinical Nephrology (74)6 2010
Abstract
A family was identified with autosomal dominant inheritance of anemia, polyuria, hyperuricemia, and chronic kidney disease. Mutational analysis revealed a novel heterozygous mutation c.58T > C resulting in the amino acid substitution of cysteine for arginine in the preprorenin signal sequence (p.cys...
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PMID: 21084044
PDF is available here.
PDF is available here.
Biochemical Journal (432)3 2010
Abstract
We generated a series of propeptide mutants to determine the minimal requirement to prevent PC-PLC enzymatic activity and to identify residues regulating compartmentalization and maturation. We found that a single residue at position P1 (Ser51) of the cleavage site is sufficient to prevent enzymatic...
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PMID: 20879990
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)45 2010
Abstract
We now performed pulldown experiments to systematically map the differential binding of adaptor proteins of the endosomal sorting machinery (adaptor proteins and GGAs (Golgi-localized, γ-ear containing, Arf binding)) as well as clathrin to the cytosolic regions of the intracellular CLCs. The result...
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PMID: 20817731
PDF is available here.
PDF is available here.
Abstract
Abstract
Although many antimicrobial components (i.e. antimicrobial peptides) have been found in many social Hymenoptera venoms, no antimicrobial compound is purified and characterized from parasitic Hymenoptera. From the venoms of the ectoparasitic wasp, Nasonia vitripennis, a defensin-like antimicrobial pe...
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PMID: 20362606
PDF is available here.
PDF is available here.
Abstract
We expressed Abeta peptide as a new causing agent to endoplasmic reticulum (ER) stress to study ER stress occurred in plant. When the dimer of Abeta(1-42) peptide was expressed in maturing seed under the control of the 2.3-kb glutelin GluB-1 promoter containing its signal peptide, a maximum of about...
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PMID: 20331531
PDF is available here.
PDF is available here.
Journal of General Virology (91)Pt 8 2010
Abstract
Cell-to-cell movement of potexviruses requires coordinated action of the coat protein and triple gene block (TGB) proteins. The structural properties of Alternanthera mosaic virus (AltMV) TGB3 were examined by methods differentiating between signal peptides and transmembrane domains, and its subcell...
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PMID: 20392901
PDF is available here.
PDF is available here.
Infection and Immunity (78)8 2010
Abstract
We further characterize this domain by identifying six residues that are essential for ExoU activity. Substitutions at each of these positions resulted in abrogation of membrane targeting, decreased ExoU-mediated cytotoxicity, and reductions in PLA(2) activity. Likewise, each of the six MLD residues...
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PMID: 20479080
PDF is available here.
PDF is available here.
Abstract
Endoglucanase is a major cellulolytic enzyme produced by the fungus Trichoderma viride. The 1,317 bp cDNA of endoglucanase gene egVIII was cloned from T. viride AS3.3711, encoding a 438 amino acid protein with a calculated molecular mass of 46.86 kDa and isoelectric point of 4.32. Sequence analysis...
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PMID: 19590984
PDF is available here.
PDF is available here.
Abstract
We searched the predicted Theileria annulata and T. parva proteomes for putative schizont surface proteins. This led to the identification of gp34, a GPI-anchored protein that is stage-specifically expressed by schizonts of both Theileria species and is downregulated upon induction of merogony. Tran...
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PMID: 20381541
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)29 2010
Abstract
We demonstrated that protein kinase Cdelta (PKCdelta) inhibits F(1)F(0) activity via an interaction with the "d" subunit of F(1)F(0)-ATP synthase (dF(1)F(0)) in neonatal cardiac myocytes (NCMs) (Nguyen, T., Ogbi, M., and Johnson, J. A. (2008) J. Biol. Chem. 283, 29831-29840). We have now identified...
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PMID: 20460381
PDF is available here.
PDF is available here.
Journal of Cell Science (123)Pt 14 2010
Abstract
Abstract
We present here, an HMM that combine a transmembrane barrel submodel and an SP submodel for both topology and SP predictions. A new genetic algorithm (GA) is presented here to training the model, at the same time the Posterior-Viterbi algorithm is adopted for decoding. A dataset including 33 TMBs th...
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PMID: 20488436
PDF is available here.
PDF is available here.
Journal of Virology (84)13 2010
Abstract
We found that widespread mitochondrial distribution occurs within 1 h of pUL37x1 synthesis. The pUL37x1 mitochondrial targeting signal (MTS) spans its first antiapoptotic domain (residues 5 to 34) and consists of a weak hydrophobicity leader (MTSalpha) and proximal downstream residues (MTSbeta). Thi...
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PMID: 20410282
PDF is available here.
PDF is available here.
Abstract
We found that EspJ was unstable in host cells, which might be attributable to the N-terminal part beginning from amino acid number 59. Using stable forms of EspJ derivatives, we demonstrated for the first time that EspJ has the ability to translocate into mitochondria via an atypical mitochondrial t...
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PMID: 20618683
PDF is available here.
PDF is available here.
Abstract
We investigated the signals that determine apical expression of NaPi-IIc with a focus on the role of the N- and the C-terminal tails of mouse NaPi-IIc in renal epithelial cells [opossum kidney (OK) and Madin-Darby canine kidney cells]. Wild-type NaPi-IIc, the cotransporter NaPi-IIa, as well as sever...
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PMID: 20410212
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
A chimeric mammalian globular cytochrome b(5) fused to Escherichia coli alkaline phosphatase signal sequence (SS) was used as a model probe to investigate the influence of substituting each one of the standard 20 amino acids at its N-terminus on the Sec-dependent export of the precur...
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PMID: 20506367
PDF is available here.
PDF is available here.
Archives of Microbiology (192)7 2010
Abstract
Pseudomonas fluorescens GcM5-1A was isolated from the pine wood nematode (PWN), Bursaphelenchus xylophilus, obtained from wilted Japanese black pine, Pinus thumbergii, in China. In this paper, a genomic library of the GcM5-1A strain was constructed and a toxin-producing clone was isolated by bioassa...
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PMID: 20495785
PDF is available here.
PDF is available here.
Abstract
We have produced a fusion protein between Sulfolobus solfataricus SRP54 (Ffh) and a signal peptide connected via a flexible linker. This fusion protein oligomerizes in solution through interaction between the SRP54 and signal peptide moieties belonging to different chains, and it is functional, as d...
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PMID: 20364120
PDF is available here.
PDF is available here.
Abstract
Regulatory nascent chains interact with the ribosomal exit tunnel and modulate their own translation. To characterize nascent chain recognition by the ribosome at the atomic level, extensive molecular dynamics simulations of TnaC, the leader peptide of the tryptophanase operon, inside the exit tunne...
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PMID: 20462496
PDF is available here.
PDF is available here.
Abstract
We suggest a new class of peroxisomes involved in microtubule (MT) formation. Eukaryotic cells assemble MTs from distinct points in the cell. In the fungus Aspergillus nidulans, septum-associated microtubule-organizing centers (sMTOCs) are very active in addition to the spindle pole bodies (SPBs). P...
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PMID: 20348383
PDF is available here.
PDF is available here.
Abstract
We have been unable to construct the mcsADelta citADelta double mutant, and the expression of mcsA is subject to CreA-mediated carbon repression. Therefore, McsA can substitute for the loss of CitA activity. Deletion of citA does not affect conidiation or sexual development but results in delayed co...
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PMID: 20173036
PDF is available here.
PDF is available here.
Protoplasma (240)1-4 2010
Abstract
Abstract
The gene coding for rice chloroplastic L-myo-inositol-1-phosphate synthase (MIPS; EC 5.5.1.4) has been identified by matrix-assisted laser desorption time-of-flight mass spectrometry analysis of the purified and immunologically cross-reactive approximately 60 kDa chloroplastic protein following two-...
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PMID: 20213122
PDF is available here.
PDF is available here.
Microbiology (156)Pt 4 2010
Abstract
These results indicate that secretion and translocation signals are present in the N terminus of DspA/E, and that at least one DspB/F-binding motif is required for efficient translocation into plant cells....
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PMID: 20110301
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (26)4 2010
Abstract
The 293FT cells infected by the recombinant lentivirus could express the fused protein SP-TAT-Apoptin. Anexin-V PI assay showed that SP-TAT-Apoptin carried by the recombinant lentivirus could cause the HepG2 cell apoptosis, and its apoptosis rate was significantly more than paired control group and...
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PMID: 20368104
PDF is available here.
PDF is available here.
Journal of Biochemistry (147)4 2010
Abstract
We report on a novel C1q family protein specifically expressed in the central nervous system (CNS), which we designated neural C1q-like protein (nCLP) 2. nCLP2 was secreted as disulphide-bonded multimers comprising trimeric units. The multimers were stabilized by interchain disulphide bonds involvin...
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PMID: 19996152
PDF is available here.
PDF is available here.
Journal of Biochemistry (147)4 2010
Abstract
We examined organelle-targeting properties of the N-terminal portions of the peroxisomal isoform of the ABC transporter PMP70 (ABCD3) using enhanced green fluorescent protein (EGFP) fusion. When the N-terminal 80 amino acid residue (N80)-segment preceding transmembrane segment (TM) 1 was deleted and...
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PMID: 20007743
PDF is available here.
PDF is available here.
Journal of Biochemistry (147)4 2010
Abstract
We have identified a putative sialidase gene, nanH, from C. diphtheriae KCTC3075 and characterized its product for enzyme activity. Interestingly, the recombinant NanH protein was secreted as a catalytically active sialidase into the periplasmic space in Escherichia coli, while the short region at i...
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PMID: 20007980
PDF is available here.
PDF is available here.
Methods in Molecular Biology (607)607 2010
Abstract
We should obtain mg quantities of (15)N and/or (13)C labeled pure proteins of interest. Here, we describe the method to produce dual amino acid-selective (13)C-(15)N labeled proteins for NMR study using the improved wheat germ cell-free system, which enables sequence-specific assignments of amide si...
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PMID: 20204853
PDF is available here.
PDF is available here.
Molecular Biotechnology (44)3 2010
Abstract
We applied a directed evolution strategy to improve the oligovalent display of a poorly behaving Fab fragment fused to phage gene-3 for minor coat protein (g3p). The Fab displaying clones were enriched from a randomly mutated Fab gene library with polyclonal anti-mouse IgG antibodies. Contribution o...
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PMID: 20012710
PDF is available here.
PDF is available here.
Abstract
We show that the protein responsible for cleavage of this motif is plasmepsin V (PMV), an aspartic acid protease located in the endoplasmic reticulum. PMV cleavage reveals the export signal (xE/Q/D) at the amino terminus of cargo proteins. Expression of an identical mature protein with xQ at the N t...
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PMID: 20130643
PDF is available here.
PDF is available here.
Abstract
We report that plasmepsin V, an ER aspartic protease with distant homology to the mammalian processing enzyme BACE, recognizes the PEXEL motif and cleaves it at the correct site. This enzyme is essential for parasite viability and ER residence is essential for its function. We propose that plasmepsi...
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PMID: 20130644
PDF is available here.
PDF is available here.
Abstract
We observed, however, that the protein downstream of 2A (no signal) was translocated into the endoplasmic reticulum (ER). We interpreted these data as a form of 'slipstream' translocation: downstream proteins, without signals, were translocated through a translocon pore already formed by the signal...
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PMID: 19946875
PDF is available here.
PDF is available here.
Abstract
We have demonstrated that the 26-47 segment of Salmonella enterica serovar Typhimurium flagellin is capable of mediating flagellar export. In order to reveal whether other parts of the N-terminal region have any significant influence on secretion, a series of plasmids were constructed containing the...
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PMID: 20008166
PDF is available here.
PDF is available here.
Abstract
We investigated the contributions of the pre (pre(lip)) and pro (pro(lip)) sequences of a Staphylococcus hyicus lipase to secretion of a heterologous protein, the alkaline phosphatase PhoA of Escherichia coli, by B. subtilis. The results indicate that the presence of the pro(lip)-peptide, in combina...
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PMID: 19948853
PDF is available here.
PDF is available here.
Journal of Virology (84)4 2010
Abstract
We performed genetic analyses to map the essential determinants of the HCV core C-terminal region, as well as to define the minimal length of the protein that can function for infectious virus production in trans....
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PMID: 20007277
PDF is available here.
PDF is available here.
Abstract
We demonstrated that conformational changes were sufficient to cause Bax to move from the cytosol to the mitochondria. Moreover, we found that homo-oligomerization of Bax contributed to its mitochondrial translocation. These results suggest that Bax is targeted to the mitochondria through the exposu...
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PMID: 19880508
PDF is available here.
PDF is available here.
Abstract
The cycP gene encoding a periplasmic cytochrome c' from the denitrifying beta-proteobacterium Achromobacter xylosoxidans was characterized. The genes flanking cycP encode components of a mobile genetic element characteristic of the beta-proteobacteria, suggesting that cycP has inserted within a tran...
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PMID: 20185933
PDF is available here.
PDF is available here.
Abstract
We have extended these in vivo analyses to additional Hfx. volcanii substrates, supporting our previous in silico predictions and confirming the diversity of predicted Hfx. volcanii lipoproteins. Moreover, using extensive comparative secretome analyses, we identified genes encodining putative lipopr...
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PMID: 20886060
PDF is available here.
PDF is available here.
Abstract
The skc gene encoding streptokinase (SK), with a molecular weight of approximately 47.4 kDa, was cloned from Streptococcus eouisimilis ATCC9542 and heterologously overexpressed in Streptomyces lividans TK24 and E. coli using various strong promoters. When the sprT promoter was used in the S. lividan...
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PMID: 20134244
PDF is available here.
PDF is available here.
Abstract
We have accumulated distinct transport intermediates of preproteins to analyze the translocases in their active, preprotein-carrying state. We identified two different forms of active TOM-TIM23 supercomplexes, TOM-TIM23(SORT) and TOM-TIM23-PAM. These two supercomplexes do not represent separate path...
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PMID: 19884344
PDF is available here.
PDF is available here.
Abstract
We isolated mitochondrial fractions from yeast cells lacking the major import receptor, Tom20, and compared their mRNA repertoire to that of wild-type cells by DNA microarrays. Most mRNAs encoding mitochondrial proteins were less associated with mitochondria, yet the extent of decrease varied among...
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PMID: 19858288
PDF is available here.
PDF is available here.
Abstract
The filamentous ascomycete Sclerotinia sclerotiorum is well known for its ability to produce a large variety of hydrolytic enzymes for the degradation of plant polysaccharide material. Two alpha-amylases designated as ScAmy54 and ScAmy43 were biochemically characterized and predicted to play an impo...
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PMID: 19996681
PDF is available here.
PDF is available here.
Abstract
Our results also indicated that tatC is essential for C. jejuni to sustain colonization in chickens. These findings suggest that the TAT pathway affects Campylobacter physiology and contributes to stress responses, allowing this fastidious pathogen to adapt to various environmental conditions....
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PMID: 19799526
PDF is available here.
PDF is available here.
Abstract
The alginate lyase encoding gene (alyPI) of marine bacterium Pseudoalteromonas sp. CY24 was cloned using a battery of PCR techniques. Gene alyPI was composed of a 1575 bp open reading frame encoding a protein of 57.4 kDa containing 524 amino acid residues with a signal peptide of 23 amino acids. The...
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PMID: 19898554
PDF is available here.
PDF is available here.
Abstract
Dirofilaria immitis is an important filarial parasite of dogs and cats, and a useful model for human filariasis. Current diagnostic tests for heartworm infection in animals rely on the presence of fecund female worms (usually found 6.5 months post-infection or later) and therefore fail to detect pre...
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PMID: 8728997
PDF is available here.
PDF is available here.
Abstract
I transmembrane protein. It includes an N-terminal signal sequence, a carbohydrate recognition domain, which is calcium-dependent and pH-sensitive, a stalk region, a transmembrane domain, and a short cytoplasmic domain; ERGIC-53 mainly acts as a receptor of a limited number of glycoprotein and trans...
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PMID: 19609866
PDF is available here.
PDF is available here.
Abstract
Because of its eukaryotic nature, simple fermentation requirements, and pliable genetics, there have been many attempts at improving recombinant protein production in Saccharomyces cerevisiae. These strategies typically involve altering the expression of a native protein thought to be involved in he...
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PMID: 19459139
PDF is available here.
PDF is available here.
Biotechnology Letters (31)8 2009
Abstract
Our results demonstrated the potential usefulness of the root-specific promoter for the exudation of recombinant phytases and offered a new perspective on the mobilization of phytate in soil to inorganic phosphates for plant uptake....
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PMID: 19357813
PDF is available here.
PDF is available here.