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Matrix Attachment Regions (0):
Recent article
Matrix Attachment Regions
Abstract
Abnormal trophoblast invasion is associated with the most common and most severe complications of human pregnancy. The biology of invasion, as well as the etiology of abnormal invasion remains poorly understood. The aim of this study was to characterize the transcriptome of the HTR-8/SVneo human cyt...
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PMID: 20346505
PDF is available here.
PDF is available here.
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Molecular and Cellular Biochemistry (336)1-2 2010
Abstract
The orchestration of the events in the cell during the progression of the cell cycle is modulated by various phenomenon which are regulated by structural modules of the cell. The nucleus is a major hub for all these regulatory units which harbour the nuclear matrix, matrix proteins and chromatin. Th...
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PMID: 19802523
PDF is available here.
PDF is available here.
Abstract
We have also shown that the transgene expression level is not directly proportional to the gene copy number, and gene copy number dependency does not exist....
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PMID: 19947951
PDF is available here.
PDF is available here.
BMC Genomics (11) 2010
Abstract
We have analysed the Giardia genome for the probable S/MARs predicted by the available computational tools; and then shown these sequences to be physically associated with the nuclear matrix. Our study also reflects that while no single computational tool is competent to predict such complex element...
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PMID: 20565887
PDF is available here.
PDF is available here.
Abstract
The clinical application of gene therapy has become a reality with the treatment of patients with X-linked SCID (SCID-X1) using a modified retrovirus. This success has been tempered by the toxicity of the vector used in this trial, which led to oncogenesis in several of the treated patients. The dev...
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PMID: 19649988
PDF is available here.
PDF is available here.
Abstract
We describe rare diagnosis of a complicated fetal ovarian cyst with edema of the labia and moderate ascites. The neonate had ambiguous genitalia with clitoromegaly. The newborn underwent surgery with oophorectomy. During the operation a uterus with fallopian tubes was found. The pathological finding...
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PMID: 18008216
PDF is available here.
PDF is available here.
Transgenic Research (18)3 2009
Abstract
We have carried out a more detailed analysis of the DNA elements in TM2 with the aim of improving its effect on transcription activation. Our study of the location effect of individual MARs on the expression of the adjacent 35S:gusA cassette indicated that the TM2 functions in a bidirectional manner...
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PMID: 19043795
PDF is available here.
PDF is available here.
Gene Therapy (15)24 2008
Abstract
We report the development of a non-viral episomal plasmid DNA (pDNA) vector that appears to fulfil these criteria. This pDNA vector combines a scaffold/matrix attachment region (S/MAR) with a human liver-specific promoter (alpha1-antitrypsin (AAT)) in such a way that long-term expression is enabled...
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PMID: 18633447
PDF is available here.
PDF is available here.
Genome Research (18)11 2008
Abstract
We have identified genomic anchors that define the organization of chromatin loop domains across the human major histocompatibility complex (MHC). This locus contains critical genes for immunity and is associated with more diseases than any other region of the genome. Classical MHC genes are express...
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PMID: 18849521
PDF is available here.
PDF is available here.
Current Gene Therapy (8)5 2008
Abstract
Matrix attachment regions (MARs) are DNA sequences that may be involved in anchoring DNA/chromatin to the nuclear matrix and they have been described in both mammalian and plant species. MARs possess a number of features that facilitate the opening and maintenance of euchromatin. When incorporated i...
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PMID: 18855632
PDF is available here.
PDF is available here.
Transgenic Research (17)4 2008
Abstract
We studied 56 independent poplar RNAi transgenic events in the field over 2 years. A resident BAR transgene was targeted with two different types of RNAi constructs: a 475-bp IR of the promoter sequence and a 275-bp IR of the coding sequence, each with and without the presence of flanking matrix att...
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PMID: 17929189
PDF is available here.
PDF is available here.
Abstract
We designed a short hairpin RNA (shRNA) expression vector called pEPI-RNAi for HBV suppression. HBV-replicating HepG2.2.15 cells were transfected with pEPI-RNAi, and the intracellular status of the plasmid was followed by PCR and Southern analysis. HBV replication was measured on the DNA, RNA, and p...
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PMID: 18474581
PDF is available here.
PDF is available here.
Abstract
We assayed gephyrin protein in two brain areas susceptible to neuronal loss in AD, and in a spared area, in autopsy tissue from normal subjects (n=15) and AD patients (n=5). Quantification was by in-gel immunodetection against known concentrations of a recombinant truncated gephyrin standard. Gephyr...
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PMID: 18599957
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)27 2008
Abstract
We previously showed that genomic imprinting regulates matrix attachment region activities at the mouse Igf2 (insulin-like growth factor 2) locus and that these activities are functionally linked to neighboring differentially methylated regions (DMRs). Here, we investigate the similarly structured D...
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PMID: 18458080
PDF is available here.
PDF is available here.
Abstract
We have sequenced the full-length cDNA and the gene encoding the precursor of denmotoxin. This is the first glimpse of genomic organization of a colubrid three-finger toxin. Denmotoxin cDNA shows low similarity to elapid three-finger toxins, except for the conserved signal peptide region. The open r...
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PMID: 18343233
PDF is available here.
PDF is available here.
Nucleic Acids Research (36)7 2008
Abstract
We have determined its optimal DNA-binding sequence by random oligonucleotide selection. The consensus SATB1-binding sequence (CSBS) comprises a palindromic sequence in which two identical AT-rich half-sites are arranged as inverted repeats flanking a central cytosine or guanine. Strikingly, the CSB...
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PMID: 18187506
PDF is available here.
PDF is available here.
Abstract
Adipose-derived stem cells (ASCs) display multilineage plasticity and, under appropriate conditions, can mineralize their extracellular matrix and undergo osteogenesis. The aims of this study are to examine in vitro osteogenic differentiation properties of ASCs to assess the role of gender, fat depo...
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PMID: 18443514
PDF is available here.
PDF is available here.
Genome Research (18)1 2008
Abstract
We demonstrated the existence in the vicinity of the D4Z4 repeat of a nuclear matrix attachment site, FR-MAR, efficient in normal human myoblasts and nonmuscular human cells but much weaker in muscle cells from FSHD patients. We now report that the D4Z4 repeat contains an exceptionally strong transc...
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PMID: 18032730
PDF is available here.
PDF is available here.
Abstract
There is increasing awareness that processes, such as development, aging and cancer, are governed, to a considerable extent, by epigenetic processes, such as DNA and histone modifications. The sites of these modifications in turn reflect their position and role in the nuclear architecture. Since epi...
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PMID: 18491049
PDF is available here.
PDF is available here.
Abstract
These results indicate that ARS905 enhances the expression of the EGFP gene and that it should be as a component of expression vectors in further fish biotechnological studies....
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PMID: 17586046
PDF is available here.
PDF is available here.
Laryngoscope (117)10 2007
Hyaluronan-based scaffolds to tissue-engineer cartilage implants for laryngotracheal reconstruction.
Abstract
None of the 18 rabbits showed signs of respiratory distress. A smooth, noninflammatory scar was visible intraluminally. Histologically, the native auricular cartilage implants showed excellent integration without any signs of inflammation or cartilage degradation. In contrast, all tissue-engineered...
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PMID: 17690606
PDF is available here.
PDF is available here.
Abstract
We previously showed that plasmids containing a mammalian replication initiation region and a matrix attachment region were efficiently amplified to few thousand copies per cell, and that they formed extrachromosomal double minutes (DMs) or chromosomal homogeneously staining regions (HSRs). In these...
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PMID: 17390337
PDF is available here.
PDF is available here.
Abstract
Nuclear matrix attachment regions (MARs) are known to bind specifically to the nuclear scaffold and are thought to influence expression of the transgenes. In our previous studies, a new deoxyribonucleic acid fragment isolated from Dunaliella salina could bind to the nuclear matrix in vitro and had t...
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PMID: 17611755
PDF is available here.
PDF is available here.
Transgenic Research (16)4 2007
Abstract
We describe the transgenic expression of a previously identified seed-specific and a so far uncharacterized plant defensin gene in their host A. thaliana using a formerly developed plant expression system. Therefore, both genes were cloned in a matrix attachment region (MAR) based plant transformati...
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PMID: 17180735
PDF is available here.
PDF is available here.
Abstract
We have identified a significant portion of the scaffold/matrix attachment regions (S/MARs) within this region. Forty independent putative S/MAR elements were assigned within the 16q22.1 locus. More than 90% of these S/MARs are AT rich, with GC contents as low as 27% in 2 cases. Thirty-nine (98%) of...
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PMID: 17188460
PDF is available here.
PDF is available here.
Regulation of expression of 1,25D3-MARRS/ERp57/PDIA3 in rat IEC-6 cells by TGF beta and 1,25(OH)2D3.
Abstract
We examined the transcriptional regulation of expression of the redox-sensitive Membrane-Associated-Rapid Response, Steroid-binding (1,25D(3)-MARRS) protein specific for 1,25(OH)(2)D(3) in a rat small intestinal cell line, IEC-6, that demonstrates rapid responses to 1,25(OH)(2)D(3). 1,25D(3)-MARRS b...
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PMID: 17188725
PDF is available here.
PDF is available here.
Abstract
The H1 complement of native chromatin was perturbed by adding an additional amount of one of the subtypes. A certain amount of SAR (scaffold-associated region) DNA was present in the mixture to avoid precipitation of chromatin by excess H1. SAR DNA also provided a set of reference relative affinitie...
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PMID: 17498293
PDF is available here.
PDF is available here.
BMC Genomics (8)1 2007
Abstract
Using custom software, we have mapped the occurrence of MRS across the entire C. elegans genome. We find that MRS have a distinctive chromosomal distribution, in which they appear more frequently in the gene-rich chromosome centres than in arms. Comparison to distributions of MRS estimated from chro...
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PMID: 18005410
PDF is available here.
PDF is available here.
Genes and Immunity (8)1 2007
Abstract
We analyzed nuclear matrix attachment regions (MARs) in the Ifng gene by DNA array technique in unactivated and activated CD4+ Th cells. This approach was combined with analysis of spatial organization of the Ifng gene by chromosome conformation capture approach to assess the relationship between th...
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PMID: 17093503
PDF is available here.
PDF is available here.
Abstract
We discuss the role of MARs and MARBPs in eukaryotic gene regulation, recombination, transcription and viral integration by altering the local chromatin structure and their dysregulation in disease manifestation...
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PMID: 17484130
PDF is available here.
PDF is available here.
Nucleic Acids Research (35)15 2007
Abstract
We describe the development of a high capacity episomal vector system exploiting human episomal retention sequences to provide efficient vector maintenance and regulated gene expression through the delivery of a genomic DNA locus. The iBAC-S/MAR vector is capable of the infectious delivery and reten...
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PMID: 17675302
PDF is available here.
PDF is available here.
Nucleic Acids Research (35)15 2007
Abstract
We have revealed a five-helix structure of the N-terminal CUT domain, which is essentially the folded region in the MAR-binding domain, of human SATB1 by NMR. Here we determined crystal structure of the complex of the CUT domain and a MAR DNA, in which the third helix of the CUT domain deeply enters...
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PMID: 17652321
PDF is available here.
PDF is available here.
Abstract
Cell Biology International (30)12 2006
Abstract
We studied the extracted cell morphology and distribution of a major scaffold component - topoisomerase IIalpha, in extracted metaphase and anaphase cells. An extraction using 2M NaCl leads to destruction of chromosomes at the light microscope level. Immunogold studies demonstrate that the only resi...
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PMID: 17029868
PDF is available here.
PDF is available here.
Abstract
We have found and characterized an unusual extended area of DNA association with the nuclear matrix in the human dystrophin gene. This extended DNA loop anchorage region (LAR) has been mapped and characterized using a variety of biochemical and microscopy techniques. It spans approximately 200 kbp a...
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PMID: 16883579
PDF is available here.
PDF is available here.
Molecular Pharmacology (70)4 2006
Abstract
We examined the role of the caveolin-1 scaffolding domain (CSD) in regulating the SOC [i.e., transient receptor potential channel-1 (TRPC1)] in human pulmonary artery endothelial cells (HPAECs). We used the cell-permeant antennapedia (AP)-conjugated CSD peptide, which competes for protein binding pa...
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PMID: 16822931
PDF is available here.
PDF is available here.
Molecular Pharmacology (70)4 2006
Abstract
We know that the main structural protein of caveolae is caveolin, how caveolin interacts with membrane proteins to facilitate their integration into lipid raft domains is unclear. A caveolin-scaffolding domain (CSD) on caveolin itself can associate with membrane proteins such as G proteins and endot...
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PMID: 16873578
PDF is available here.
PDF is available here.
Plant Journal (47)5 2006
Abstract
We generated large numbers of transgenic barley lines carrying a single copy of the non-autonomous maize Ds element at defined positions in the genome. Independent Ds lines were either generated by activating Ds elements in existing single-copy lines after crossing with AcTPase-expressing plants or...
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PMID: 16889649
PDF is available here.
PDF is available here.
Abstract
We find evidence for one highly preferred initiation site and two less utilized sites within a 6 kb region of the NTS. The initiation sites colocalize with significant DNA unwinding elements (DUEs), matrix attachment regions (MARs), and ARS-like sequences. An intrinsic DNA bending site was localized...
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PMID: 16721058
PDF is available here.
PDF is available here.
Biochemistry (New York) (71)5 2006
Abstract
We review the biological importance of the co-localization between bent DNA sites and S/MARs....
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PMID: 16732725
PDF is available here.
PDF is available here.
Abstract
We describe a vector incorporating the human 7SK promoter for shRNA-mediated gene silencing in the P19 embryonic carcinoma stem cell line. Our preliminary experiments with the 7SK shRNA expression vector indicated that its activity could be hindered by random genomic integration. In order to counter...
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PMID: 16439020
PDF is available here.
PDF is available here.
Journal of Molecular Biology (358)2 2006
Abstract
We have shown that S/MARs do share a characteristic structural property, they have a markedly high predicted propensity to undergo strand separation when placed under negative superhelical tension. This result agrees with experimental observations, that S/MARs contain base-unpairing regions (BURs)....
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PMID: 16516920
PDF is available here.
PDF is available here.
Abstract
We show here that a significant amount of Bright resides in the cytoplasm of normal and transformed B cells. Leptomycin B, chromosome region maintenance 1 (CRM1) overexpression, and heterokaryon experiments indicate that Bright actively shuttles between the nucleus and the cytoplasm in a CRM1-depend...
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PMID: 16507996
PDF is available here.
PDF is available here.
Abstract
We demonstrate that the 3'-enhancer is not only required for gene activation but is also essential for the maintenance of gene expression. Deletion of the MAR on the other hand increases IgL transcription, indicating that the MAR acts as negative regulator. We demonstrate that Id1 and Id3, dominant-...
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PMID: 16342328
PDF is available here.
PDF is available here.
Abstract
We tested the hypothesis that an anatomic scaffold placed in continuity with viable bowel might allow intestinal growth. Male ACI rats were used for the study. Acellular human dermis in the form of tubular scaffolds with an intraluminal diameter of approximately 0.3 cm was oriented with the luminal...
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PMID: 16908302
PDF is available here.
PDF is available here.
Abstract
The aim of the study was to evaluate the use of Surgisis (Cook Biotech Inc.), a porcine derived extracellular matrix already used in tissue engineering, as a scaffold for neointestinal regeneration in a rat model. A 3-cm length of tubular Surgisis graft was interposed with bilateral anastomoses in t...
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PMID: 16908301
PDF is available here.
PDF is available here.
Abstract
We summarize the biochemical evidence for the modalities of DNA-matrix binding alongside the in-silico predictions. Concordance is exhibited at various, but not all levels. On the one hand, both the reiteration and sequence similarity of some elements of Matrix Attachment Regions suggest conservatio...
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PMID: 16847565
PDF is available here.
PDF is available here.
Abstract
We report the first example of an Alu-mediated VWF gross gene deletion. Since a number of recombination-associated motifs were also identified in the vicinity of the breakpoints, it may be that multiple sequence elements have acted in concert to give rise to this deletion event....
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PMID: 16690331
PDF is available here.
PDF is available here.
Gene Therapy (13)1 2006
Abstract
We investigated the potential of this vector, pEPI-eGFP, to mediate gene transfer in hematopoietic progenitor cell lines and primary human cells. pEPI-eGFP was episomally maintained and conferred sustained eGFP expression even in nonselective conditions in the human cell line, K562, as well as in pr...
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PMID: 16094410
PDF is available here.
PDF is available here.
Hereditas (Beijing) (28)1 2006
Abstract
I and Xho I to 5' upstream of sensitive and antisensitive primers respectively, expressing vector BE was constructed after BMAR was cloned into the downstream of the reporter gene in pEGFP-C1. Bovine ear fibroblast cells were transfected by expressing vector BE with Lipofectamine.Compared with contr...
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PMID: 16469713
PDF is available here.
PDF is available here.
Acta Genetica Sinica (32)12 2005
Abstract
Nuclear DNA of eukaryotic organism attaches to the proteinaceous nuclear matrices via specific matrix attachment regions (MARs). In order to investigate the interactions between chromosomal DNA and nuclear matrices,we isolated the MARs from unicellular alga Dunaliella salina. As the first step,a ran...
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PMID: 16459661
PDF is available here.
PDF is available here.
Genetika (Moskva) (41)11 2005
Abstract
The bithorax (BX) complex of Drosophila is a complex polygenic region with a multifactorial system of regulation. One of the levels of the regulatory system of the BX complex is its association with the nuclear skeleton structures through a specific interaction of the M/SAR DNA with the nuclear matr...
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PMID: 16358714
PDF is available here.
PDF is available here.
Abstract
We analyzed the integrity of c-myc S/MAR containing a base-unpairing region (BUR)-like element, which we established to be a binding site of the transcription factor Sox2. An accumulation of DNA breaks in close proximity to this element and a degradation of Sox2 were observed early in the OGD-induce...
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PMID: 15920534
PDF is available here.
PDF is available here.
Abstract
These results strongly suggest that the characteristic organization of interphase chromosomes is supported by the proteinous nuclear matrix and is not maintained by simple repulsing of negatively charged chromosomes.
Copyright 2005 Wiley-Liss, Inc....
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PMID: 16149066
PDF is available here.
PDF is available here.
Abstract
We obtained. These plant expression vectors with one or two MARs were transformed into tobaccos via Agrobacterium-mediated transformation method, with the plant expression vector pCAMBIA2301 without MAR and wild type tobacco as controls. GUS histochemical staining results showed that the uidA gene e...
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PMID: 16468355
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (280)40 2005
Abstract
We show that SIP engages Siah1 by means of two elements, both of which are required for mediating beta-catenin destruction in cells. An N-terminal dimerization domain of SIP sits across the saddle-shaped upper surface of Siah1, with two extended legs packing against the sides of Siah1 by means of a...
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PMID: 16085652
PDF is available here.
PDF is available here.
Abstract
A major function of J-C intronic matrix attachment regions (MAR) during immune diversification via somatic hypermutation (SHM) at immunoglobulin loci may be to manipulate the topology of DNA within the upstream target domain. The suggestion that SHM induction requires MAR-induced torsional strain, i...
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PMID: 16033533
PDF is available here.
PDF is available here.
Abstract
We have evaluated their use in various stable transfection settings to increase the production of recombinant proteins such as monoclonal antibodies from Chinese hamster ovary (CHO) cell lines. Using the green fluorescent protein coding sequence, we show that S/MAR elements mediate a dual effect on...
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PMID: 15889435
PDF is available here.
PDF is available here.
Plant Cell Reports (24)3 2005
Abstract
We transformed rice with nine different constructs containing seven and six different promoters and coding sequences, respectively. Genomic Southern blot analyses of 357 independent transgenic lines revealed that in the presence of BP-MAR, 57% of the lines contained a single copy of the transgene, w...
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PMID: 15714322
PDF is available here.
PDF is available here.
Abstract
Early in female mammalian embryogenesis, one of the two X chromosomes is inactivated to compensate the gene dosage between males and females. One of the features of X chromosome inactivation (XCI) is the late replication of the inactivated X chromosome. This study reports the identification, by comp...
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PMID: 15779006
PDF is available here.
PDF is available here.