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Insulator Elements (0):
Recent article
Insulator Elements
Chromosoma (119)3 2010
Abstract
We have found an insulator, named Wari, located on the 3' side of the white gene. Here, we show that the previously identified 368-bp core of this insulator is sufficient for blocking Polycomb response element-mediated silencing. Although Wari does not contain binding sites for known insulator prote...
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PMID: 20082086
PDF is available here.
PDF is available here.
| < More recent | Older article > |
Genetika (Moskva) (46)5 2010
Abstract
Insulators are cis-regulatory elements that prevent improper gene activation and heterochromatin spreading. An insulator is contained in the Mcp boundary from the regulatory region of the Drosophila melanogaster Abd-B gene. The barrier function of the Mcp insulator was studied and proved to be due t...
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PMID: 20583593
PDF is available here.
PDF is available here.
Abstract
We explore what is known of the cell cycle dependence of epigenetic inheritance with particular emphasis on yeast loci and discuss the role of specific proteins responsible for the establishment and maintenance of these states....
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PMID: 20168086
PDF is available here.
PDF is available here.
Chromosoma (119)3 2010
Abstract
We have found an insulator, named Wari, located on the 3' side of the white gene. Here, we show that the previously identified 368-bp core of this insulator is sufficient for blocking Polycomb response element-mediated silencing. Although Wari does not contain binding sites for known insulator prote...
|
PMID: 20082086
PDF is available here.
PDF is available here.
Abstract
We investigated misexpressions that could cause the pronounced growth deficiency and death of fetuses with maternal duplication of distal chromosome (Chr) 7 (MatDup.dist7). Their small size could involve inactivity of Igf2, encoding a growth factor, with some contribution by over-expression of Cdkn1...
|
PMID: 20062522
PDF is available here.
PDF is available here.
Abstract
We have addressed the hypothesis that a barrier element in vertebrates should be capable of defending a gene from silencing by DNA methylation. Using an established stable reporter gene system, we find that HS4 acts specifically to protect a gene promoter from de novo DNA methylation. Notably, prote...
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PMID: 20062523
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)1 2010
Abstract
We analyzed the effects of interaction of two Drosophila insulators, Wari and Su(Hw). The functional interaction between these insulators was found to enhance the activity of the Su(Hw) insulator only, but not of the Wari insulator. This suggests that the formation of a chromatin loop between intera...
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PMID: 20198875
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We studied by ChIP-chip the genome-wide binding sites of 6 insulator-associated proteins-dCTCF, CP190, BEAF-32, Su(Hw), Mod(mdg4), and GAF-to obtain the first comprehensive map of insulator elements in Drosophila embryos. We identify over 14,000 putative insulators, including all classically defined...
|
PMID: 20084099
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We studied by ChIP-chip the genome-wide binding sites of 6 insulator-associated proteins-dCTCF, CP190, BEAF-32, Su(Hw), Mod(mdg4), and GAF-to obtain the first comprehensive map of insulator elements in Drosophila embryos. We identify over 14,000 putative insulators, including all classically defined...
|
PMID: 20084099
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We investigated misexpressions that could cause the pronounced growth deficiency and death of fetuses with maternal duplication of distal chromosome (Chr) 7 (MatDup.dist7). Their small size could involve inactivity of Igf2, encoding a growth factor, with some contribution by over-expression of Cdkn1...
|
PMID: 20062522
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We have addressed the hypothesis that a barrier element in vertebrates should be capable of defending a gene from silencing by DNA methylation. Using an established stable reporter gene system, we find that HS4 acts specifically to protect a gene promoter from de novo DNA methylation. Notably, prote...
|
PMID: 20062523
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We studied by ChIP-chip the genome-wide binding sites of 6 insulator-associated proteins-dCTCF, CP190, BEAF-32, Su(Hw), Mod(mdg4), and GAF-to obtain the first comprehensive map of insulator elements in Drosophila embryos. We identify over 14,000 putative insulators, including all classically defined...
|
PMID: 20084099
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We investigated misexpressions that could cause the pronounced growth deficiency and death of fetuses with maternal duplication of distal chromosome (Chr) 7 (MatDup.dist7). Their small size could involve inactivity of Igf2, encoding a growth factor, with some contribution by over-expression of Cdkn1...
|
PMID: 20062522
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We have addressed the hypothesis that a barrier element in vertebrates should be capable of defending a gene from silencing by DNA methylation. Using an established stable reporter gene system, we find that HS4 acts specifically to protect a gene promoter from de novo DNA methylation. Notably, prote...
|
PMID: 20062523
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We studied by ChIP-chip the genome-wide binding sites of 6 insulator-associated proteins-dCTCF, CP190, BEAF-32, Su(Hw), Mod(mdg4), and GAF-to obtain the first comprehensive map of insulator elements in Drosophila embryos. We identify over 14,000 putative insulators, including all classically defined...
|
PMID: 20084099
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We investigated misexpressions that could cause the pronounced growth deficiency and death of fetuses with maternal duplication of distal chromosome (Chr) 7 (MatDup.dist7). Their small size could involve inactivity of Igf2, encoding a growth factor, with some contribution by over-expression of Cdkn1...
|
PMID: 20062522
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We have addressed the hypothesis that a barrier element in vertebrates should be capable of defending a gene from silencing by DNA methylation. Using an established stable reporter gene system, we find that HS4 acts specifically to protect a gene promoter from de novo DNA methylation. Notably, prote...
|
PMID: 20062523
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We studied by ChIP-chip the genome-wide binding sites of 6 insulator-associated proteins-dCTCF, CP190, BEAF-32, Su(Hw), Mod(mdg4), and GAF-to obtain the first comprehensive map of insulator elements in Drosophila embryos. We identify over 14,000 putative insulators, including all classically defined...
|
PMID: 20084099
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We investigated misexpressions that could cause the pronounced growth deficiency and death of fetuses with maternal duplication of distal chromosome (Chr) 7 (MatDup.dist7). Their small size could involve inactivity of Igf2, encoding a growth factor, with some contribution by over-expression of Cdkn1...
|
PMID: 20062522
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We have addressed the hypothesis that a barrier element in vertebrates should be capable of defending a gene from silencing by DNA methylation. Using an established stable reporter gene system, we find that HS4 acts specifically to protect a gene promoter from de novo DNA methylation. Notably, prote...
|
PMID: 20062523
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We studied by ChIP-chip the genome-wide binding sites of 6 insulator-associated proteins-dCTCF, CP190, BEAF-32, Su(Hw), Mod(mdg4), and GAF-to obtain the first comprehensive map of insulator elements in Drosophila embryos. We identify over 14,000 putative insulators, including all classically defined...
|
PMID: 20084099
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We investigated misexpressions that could cause the pronounced growth deficiency and death of fetuses with maternal duplication of distal chromosome (Chr) 7 (MatDup.dist7). Their small size could involve inactivity of Igf2, encoding a growth factor, with some contribution by over-expression of Cdkn1...
|
PMID: 20062522
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We have addressed the hypothesis that a barrier element in vertebrates should be capable of defending a gene from silencing by DNA methylation. Using an established stable reporter gene system, we find that HS4 acts specifically to protect a gene promoter from de novo DNA methylation. Notably, prote...
|
PMID: 20062523
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We studied by ChIP-chip the genome-wide binding sites of 6 insulator-associated proteins-dCTCF, CP190, BEAF-32, Su(Hw), Mod(mdg4), and GAF-to obtain the first comprehensive map of insulator elements in Drosophila embryos. We identify over 14,000 putative insulators, including all classically defined...
|
PMID: 20084099
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We investigated misexpressions that could cause the pronounced growth deficiency and death of fetuses with maternal duplication of distal chromosome (Chr) 7 (MatDup.dist7). Their small size could involve inactivity of Igf2, encoding a growth factor, with some contribution by over-expression of Cdkn1...
|
PMID: 20062522
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We have addressed the hypothesis that a barrier element in vertebrates should be capable of defending a gene from silencing by DNA methylation. Using an established stable reporter gene system, we find that HS4 acts specifically to protect a gene promoter from de novo DNA methylation. Notably, prote...
|
PMID: 20062523
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We studied by ChIP-chip the genome-wide binding sites of 6 insulator-associated proteins-dCTCF, CP190, BEAF-32, Su(Hw), Mod(mdg4), and GAF-to obtain the first comprehensive map of insulator elements in Drosophila embryos. We identify over 14,000 putative insulators, including all classically defined...
|
PMID: 20084099
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We investigated misexpressions that could cause the pronounced growth deficiency and death of fetuses with maternal duplication of distal chromosome (Chr) 7 (MatDup.dist7). Their small size could involve inactivity of Igf2, encoding a growth factor, with some contribution by over-expression of Cdkn1...
|
PMID: 20062522
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We have addressed the hypothesis that a barrier element in vertebrates should be capable of defending a gene from silencing by DNA methylation. Using an established stable reporter gene system, we find that HS4 acts specifically to protect a gene promoter from de novo DNA methylation. Notably, prote...
|
PMID: 20062523
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We studied by ChIP-chip the genome-wide binding sites of 6 insulator-associated proteins-dCTCF, CP190, BEAF-32, Su(Hw), Mod(mdg4), and GAF-to obtain the first comprehensive map of insulator elements in Drosophila embryos. We identify over 14,000 putative insulators, including all classically defined...
|
PMID: 20084099
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We investigated misexpressions that could cause the pronounced growth deficiency and death of fetuses with maternal duplication of distal chromosome (Chr) 7 (MatDup.dist7). Their small size could involve inactivity of Igf2, encoding a growth factor, with some contribution by over-expression of Cdkn1...
|
PMID: 20062522
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We have addressed the hypothesis that a barrier element in vertebrates should be capable of defending a gene from silencing by DNA methylation. Using an established stable reporter gene system, we find that HS4 acts specifically to protect a gene promoter from de novo DNA methylation. Notably, prote...
|
PMID: 20062523
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We studied by ChIP-chip the genome-wide binding sites of 6 insulator-associated proteins-dCTCF, CP190, BEAF-32, Su(Hw), Mod(mdg4), and GAF-to obtain the first comprehensive map of insulator elements in Drosophila embryos. We identify over 14,000 putative insulators, including all classically defined...
|
PMID: 20084099
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We investigated misexpressions that could cause the pronounced growth deficiency and death of fetuses with maternal duplication of distal chromosome (Chr) 7 (MatDup.dist7). Their small size could involve inactivity of Igf2, encoding a growth factor, with some contribution by over-expression of Cdkn1...
|
PMID: 20062522
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We have addressed the hypothesis that a barrier element in vertebrates should be capable of defending a gene from silencing by DNA methylation. Using an established stable reporter gene system, we find that HS4 acts specifically to protect a gene promoter from de novo DNA methylation. Notably, prote...
|
PMID: 20062523
PDF is available here.
PDF is available here.
Abstract
Chromatin insulators are DNA-protein complexes with broad functions in nuclear biology. Drosophila has at least five different types of insulators; recent results suggest that these different insulators share some components that may allow them to function through common mechanisms. Data from genome...
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PMID: 19752045
PDF is available here.
PDF is available here.
Abstract
We show that the sea urchin chromatin insulator, sns5, protects a recombinant gamma-retroviral vector from the negative influence of chromatin in erythroid milieu. This element increases the probability of vector expression at different chromosomal integration sites, which reduces both silencing and...
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PMID: 19352322
PDF is available here.
PDF is available here.
Gene Therapy (16)7 2009
Abstract
We designed a lentiviral vector carrying two transcriptional units separated by polyadenylation, terminator and insulator sequences. With this design, the expression level of both genes was as high as that yielded from lentiviral vectors containing only a single transcriptional unit. Similar results...
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PMID: 19440229
PDF is available here.
PDF is available here.
EMBO Reports (10)7 2009
Abstract
Abstract
We will address how such a variety of apparently different genomic sequences converge in a similar function, namely, to adequately insulate a gene expression domain, thereby allowing the locus to be expressed according to their own gene regulatory elements without interfering itself and being interf...
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PMID: 19752046
PDF is available here.
PDF is available here.
EMBO Journal (28)7 2009
Abstract
We analysed genome-wide binding of CP190 and dCTCF. CP190 binding was detected at CTCF, su(Hw) and GAF sites and unexpectedly at the transcriptional start sites of actively transcribed genes. Both insulator and transcription start site CP190-binding elements are strictly marked by a depletion of his...
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PMID: 19229299
PDF is available here.
PDF is available here.
EMBO Journal (28)9 2009
Abstract
Long-range regulatory elements and higher-order chromatin structure coordinate the expression of multiple genes in cluster, and CTCF/cohesin-mediated chromatin insulator may be a key in this regulation. The human apolipoprotein (APO) A1/C3/A4/A5 gene region, whose alterations increase the risk of dy...
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PMID: 19322193
PDF is available here.
PDF is available here.
Abstract
We apply the boundary element method to describe the fluid velocity profiles in pockets in protein surfaces that are crucial to their function as enzymes. First, we study a simplified model, that of a dimpled sphere, in order to properly interpret the behavior in more complex surfaces such as protei...
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PMID: 19708204
PDF is available here.
PDF is available here.
Genetika (Moskva) (45)3 2009
Abstract
Insulators are regulatory elements having two properties. First, they are able to disturb the interaction between promoters and enhancers/silencers. Second, they are able to block distribution of the heterochromatin. The best-studied are the Su(Hw)-dependent insulators of Drosophila melanogaster, ac...
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PMID: 19382683
PDF is available here.
PDF is available here.
BMC Biotechnology (9)1 2009
Abstract
In this study we cloned in tandem copies of the chicken beta-globin insulator (cHS4) on both sides of the transgene cassette in order to enhance the insulating effect. Our insulator vectors were produced at significantly lower titers compared to control vectors, and we show that this reduction in ti...
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PMID: 19239708
PDF is available here.
PDF is available here.
Abstract
We explored the activity of human beta-globin HS5, the orthologue of the CTCF-dependent chicken HS4 insulator. An extra copy of HS5 placed between the beta-globin locus control region (LCR) and downstream genes on a transgene fulfills the classic predictions for an enhancer-blocker. Ectopic HS5 does...
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PMID: 19074263
PDF is available here.
PDF is available here.
Genetika (Moskva) (44)12 2008
Abstract
Fine regulation of complex gene loci in higher eukaryotes is realized through the interaction of promoters with enhancers and repressors, which can be located long distance from the promoter regulated. A question arises, what mechanisms determine proper contacts between the regulatory elements over...
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PMID: 19178089
PDF is available here.
PDF is available here.
Nucleic Acids Research (36)22 2008
Abstract
We have demonstrated that pairs of well-studied insulators such as scs-scs, scs'-scs', 1A2-1A2 and Wari-Wari support distant activation of the white promoter by the yeast GAL4 activator in an orientation-dependent manner. The same is true for the efficiency of the enhancer that stimulates white expr...
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PMID: 18987002
PDF is available here.
PDF is available here.
Eukaryotic Cell (7)12 2008
Abstract
We show that in Saccharomyces cerevisiae tRNA genes (tDNAs) and even partially assembled Pol III complexes containing only the transcription factor TFIIIC can exhibit chromatin boundary functions both as heterochromatin barriers and as insulators to gene activation. Both the TRT2 tDNA and the ETC4 s...
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PMID: 18849469
PDF is available here.
PDF is available here.
Molecular Cell (32)1 2008
Abstract
Enhancer-blocking insulators are DNA elements that disrupt the communication between a regulatory sequence, such as an enhancer or a silencer, and a promoter. Insulators participate in both transcriptional regulation and global nuclear organization, two features of chromatin that are thought to be m...
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PMID: 18851828
PDF is available here.
PDF is available here.
Abstract
CTCF plays diverse roles in the regulation of eukaryotic genes. A new study by Lefevre et al. in a recent issue of Molecular Cell reveals a novel mechanism in which noncoding RNA transcription and nucleosome repositioning evicts CTCF from a regulatory element to facilitate induction of a nearby gene....
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PMID: 18854131
PDF is available here.
PDF is available here.
Human Molecular Genetics (17)19 2008
Abstract
We used chromosome conformation capture to assay the native three-dimensional organization of the H19/Igf2 locus on each parental copy. Furthermore, we compared wild-type chromosomes to several mutations that affect the insulator. Our results show that promoters and enhancers reproducibly co-localiz...
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PMID: 18617529
PDF is available here.
PDF is available here.
Abstract
We previously generated transgenic mice carrying a large P1 artificial chromosome (PAC160) encompassing a 160-kb segment containing the human renin gene, two upstream genes, and one downstream gene. We also previously generated mutant PAC160 constructs lacking the distal enhancer and concluded it is...
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PMID: 18632798
PDF is available here.
PDF is available here.
Abstract
Developmental Dynamics (237)9 2008
Abstract
We have adopted an I-SceI meganuclease-mediated transgenesis method. Several types of promoter-GFP gene constructs flanked by two I-SceI recognition sequences were co-injected with I-SceI into sea urchin fertilized eggs. Using cell-lineage-specific promoter constructs, the frequency of transgene exp...
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PMID: 18729225
PDF is available here.
PDF is available here.
Abstract
We studied the interplay of different Su(Hw)-dependent Drosophila insulators. The set of transgenic constructs comprised two consecutive genes (yellow and white) with their enhancers and insulator elements differently arranged in between and/or around the gene(s). All insulators were found to intera...
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PMID: 18573869
PDF is available here.
PDF is available here.
Genome Research (18)8 2008
Abstract
We developed an experimental strategy to identify silencers and EBs using transient transfection assays. A known insulator and EB from the chicken beta-globin locus, cHS4, served as a control element for these assays. We examined 47 sequences from a 1.8-Mb region of human chromosome 7 for silencer a...
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PMID: 18436892
PDF is available here.
PDF is available here.
FASEB Journal (22)7 2008
Abstract
We show that transgenes escape this silencing in Xenopus laevis and Xenopus tropicalis embryos and that a stable, uniform, and heritable expression pattern is obtained when transgenes are flanked with tandem copies of the chicken beta-globin 5'HS4 insulator. Our data also indicate that the insulator...
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PMID: 18359926
PDF is available here.
PDF is available here.
Abstract
We study SI 62D and show that interactions occur between two SI 62D elements, but not between SI 62D and the gypsy insulator, limiting the scope of genomic gypsy insulator interactions. Enhancer blocking by SI 62D requires fewer Su(Hw)-binding sites than needed for gypsy insulator function, with the...
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PMID: 18562648
PDF is available here.
PDF is available here.
Abstract
We show that the STAG1 (Scc3/SA1) subunit of cohesin interacts with the CCTC-binding factor CTCF bound to the c-myc insulator element. Both allele-specific binding of CTCF and Scc3/SA1 at the imprinted IGF2/H19 gene locus and our analyses of human DM1 alleles containing base substitutions at CTCF-bi...
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PMID: 18550811
PDF is available here.
PDF is available here.