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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Response Elements (2):
Recent article
Response Elements
Cancer Research (71)22 2011
Abstract
We developed a curated database of 88 nuclear receptor cistrome data sets and other associated high-throughput data sets including 121 collaborating factor cistromes, 94 epigenomes, and 319 transcriptomes. Through integrative analysis of the curated nuclear receptor ChIP-chip/seq data sets, we disco...
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PMID: 21940749
PDF is available here.
PDF is available here.
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Endocrinology (152)10 2011
Abstract
We characterized to which GRE GR binds in the rat hippocampus. Using a position-specific scoring matrix, we identified evolutionary-conserved putative GREs from a microarray based set of hippocampal target genes. Using chromatin immunoprecipitation, we were able to confirm GR binding to 15 out of a...
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PMID: 21846803
PDF is available here.
PDF is available here.
Abstract
We report here that clusters of charged amino acids in the TR hormone-binding domain are required for this enhanced mode of coactivator recruitment and that mutations in these charge clusters, by disrupting TRβ2 coactivator binding, are a molecular basis for pituitary resistance to thyroid hormone,...
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PMID: 21622532
PDF is available here.
PDF is available here.
Abstract
We show that hypoxia induces the expression of Snail via HIF. In silico analysis identified a potential hypoxia-response element (HRE) close to the minimal promoter of the human and mouse genome of the snail gene. Gel shift assays demonstrated that a specific hypoxia-inducible complex is formed with...
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PMID: 21257819
PDF is available here.
PDF is available here.
Abstract
We have performed genomewide analyses of agonist-treated and PPARβ/δ-depleted human myofibroblasts to test this hypothesis and to identify global principles of PPARβ/δ-mediated gene regulation. Chromatin immunoprecipitation sequencing (ChIP-Seq) of PPARβ/δ, H3K4me3 and RNA polymerase II enrich...
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PMID: 21283829
PDF is available here.
PDF is available here.
BMC Systems Biology (5)Suppl 1 2011
Abstract
Hypoxia-inducible factors (HIFs) are transcription factors that play a crucial role in response to hypoxic stress in living organisms. The HIF pathway is activated by changes in cellular oxygen levels and has significant impacts on the regulation of gene expression patterns in cancer...
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PMID: 21689478
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)52 2010
Abstract
We demonstrate that Sre1-Scp1 senses ergosterol. Processing experimental data with a mathematical model of Sre1 and Scp1 function reveals a clear quantitative relationship between ergosterol concentration in the endoplasmic reticulum and Sre1 activation. Based on this relationship, we predict that t...
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PMID: 20959444
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)52 2010
Abstract
The expression of a variety of cytoprotective genes is regulated by short cis-acting elements in their promoters, called antioxidant response elements (AREs). A central regulator of ARE-mediated gene expression is the NF-E2-related factor 2 (Nrf2). Human hepatitis B virus (HBV) induces a strong acti...
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PMID: 20956535
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)52 2010
Abstract
We recently identified Grainyhead-like 2 (GRHL2) as a novel transcription factor that binds to and regulates the activity of the human telomerase reverse transcriptase (hTERT) gene promoter. In this study, we investigated the biological functions of GRHL2 and the molecular mechanism underlying hTERT...
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PMID: 20938050
PDF is available here.
PDF is available here.
Abstract
Luciferase reporter constructs and transient co-transfection approaches demonstrate that elevated expression of RORalpha1 augments 17-beta-estradiol (E(2))-induced transcriptional activation of the full-length ERalpha, but not truncated ERalpha constructs (ABCD or CDEF), in MCF-7 breast cancer and H...
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PMID: 20558189
PDF is available here.
PDF is available here.
Abstract
Activation of the KEAP1-NRF2 signaling pathway is an adaptive response to environmental and endogenous stresses and serves to render animals resistant to chemical carcinogenesis and other forms of toxicity, whereas disruption of the pathway exacerbates these outcomes. This pathway, w...
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PMID: 20367496
PDF is available here.
PDF is available here.
Abstract
We will discuss the current progress in the study of Nrf2 signaling, in particular, the mechanisms of Nrf2 activation by chemopreventive agents. We will also discuss some of the potential caveats of Nrf2 in cancer treatment and future opportunity and challenges on regulation of Nrf2-mediated antioxi...
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PMID: 20486765
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)45 2010
Abstract
We investigated the regulatory mechanism of Srx induction by lipopolysaccharide (LPS) in mouse macrophages. LPS up-regulated Srx expression on the transcriptional level. The promoter region of the Srx gene contained putative NF-κB and AP-1 (activator protein-1) sites, and the proximal site of three...
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PMID: 20826812
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)45 2010
Abstract
We describe the isolation of the hTLR8 promoter and the characterization of the molecular mechanisms involved in its regulation. Reporter gene analysis and ChIP assays demonstrated that the hTLR8 regulation of the basal transcription is regulated via three C/EBP cis-acting elements that required C/E...
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PMID: 20829351
PDF is available here.
PDF is available here.
Abstract
We investigated NURR1, a GC-responsive transcription factor overexpressed in RA, as a MIF signaling target. We reveal abrogation by recombinant MIF (rMIF) of GC-induced MKP1 expression in RA fibroblast-like synoviocytes (FLS). rMIF enhanced NURR1 expression, artificial NBRE (orphan receptor DNA-bind...
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PMID: 20829434
PDF is available here.
PDF is available here.
Abstract
We solved the crystal structure of a Rev dimer at 2.5-Å resolution. The dimer arrangement organizes arginine-rich helices at the ends of a V-shaped assembly to bind adjacent RNA sites and structurally couple dimerization and RNA recognition. A second protein-protein interface arranges higher-order...
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PMID: 20953181
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)43 2010
Abstract
We revealed that Bmi-1 regulates the expression of p16 by binding directly to the Bmi-1-responding element (BRE) within the p16 promoter. The BRE resided at bp -821 to -732 upstream of the p16 ATG codon. BRE alone was sufficient to allow Bmi-1-mediated regulation of the CMV promoter. Bmi-1 typically...
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PMID: 20551323
PDF is available here.
PDF is available here.
Abstract
We characterized the functional domains of BFV BTas. BTas contains two major functional domains: the N-terminal DNA-binding domain (residues 1-133) and the C-terminal activation domain (residues 198-249). The complete BTas responsive regions were mapped to the positions -380/-140 of LTR and 9205/927...
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PMID: 20615521
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)10 2010
Abstract
The Tsix regulatory region was examined in vole Microtus rossiaemeridionalis. The minimal promoter region, three potential enhancer regulatory elements and one transcription suppressor element were identified. The enhancer regions contained potential binding sites of transcription activators, while...
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PMID: 21254563
PDF is available here.
PDF is available here.
Abstract
I small heat shock protein (sHSP-CI) genes were found to be selectively induced by L-azetidine-2-carboxylic acid (AZC) on chromosome 3 but not chromosome 1. Here it is shown that a novel cis-responsive element contributed to the differential regulation. By serial deletion and computational analysis,...
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PMID: 20643810
PDF is available here.
PDF is available here.
Abstract
We reported cAMP/protein kinase A pathway positively regulates PAI-1 expression through cAMP-response element binding protein binding to hypoxia response element-1 at -158 to -153bp of human PAI-1 promoter in human MCs. Moreover, cAMP synergistically augments PAI-1 expression with ionomycin- or IgE...
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PMID: 20816667
PDF is available here.
PDF is available here.
Abstract
We for the first time report the role of ecdysteroids in the regulation of hexamerin synthesis in a lepidopteran insect Corcyra cephalonica. The hormonal studies were carried out using the normal and the thorax-ligated insects with both 20E and its non-steroidal agonist RH-5992. The in vitro as well...
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PMID: 20361975
PDF is available here.
PDF is available here.
Proteomics (10)17 2010
Abstract
We investigated AR protein signaling in human peripheral blood lymphocytes treated with supraphysiological doses of DHT. We performed a comparative proteomic analysis and we identified about 30 differentially expressed proteins. At least five species contained a consensus androgen-response elements...
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PMID: 20677326
PDF is available here.
PDF is available here.
Proteomics (10)17 2010
Abstract
We investigated AR protein signaling in human peripheral blood lymphocytes treated with supraphysiological doses of DHT. We performed a comparative proteomic analysis and we identified about 30 differentially expressed proteins. At least five species contained a consensus androgen-response elements...
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PMID: 20677326
PDF is available here.
PDF is available here.
Abstract
Allergic contact dermatitis (ACD) is a significant safety concern for developers of cosmetic, personal care, chemical, pharmaceutical, and medical device products. The guinea pig maximization test (GMPT) and the murine local lymph node assay (LLNA) are accepted methods for determinin...
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PMID: 20491607
PDF is available here.
PDF is available here.
Molecular and Cellular Endocrinology (325)1-2 2010
Abstract
We characterized a binding site for PPARalpha in the second intron of the rat CPT-1A gene. Our studies indicated that WY14643 and long chain fatty acids induce CPT-1A gene expression through this element. In addition, we found that mutation of the PPARalpha binding site reduced the expression of CPT...
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PMID: 20638986
PDF is available here.
PDF is available here.
Abstract
We have investigated the influence of hypoxia on Col1 fiber density in solid breast and prostate tumor models. Second harmonic generation (SHG) microscopy was used to detect differences in Col1 fiber density and volume between hypoxic and normoxic tumor regions. Hypoxic regions were detected by fluo...
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PMID: 20689755
PDF is available here.
PDF is available here.
Abstract
We describe key aspects of the fundamentals of p53-mediated transcriptional regulation and target gene promoter selectivity....
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PMID: 20679336
PDF is available here.
PDF is available here.
Abstract
We describe key aspects of the fundamentals of p53-mediated transcriptional regulation and target gene promoter selectivity....
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PMID: 20679336
PDF is available here.
PDF is available here.
Cell Cycle (9)15 2010
Abstract
We describe here a novel, non-radioactive assay to measure p53-DNA binding which involves the sequential use of in vitro transcription/ translation (IVT), immunoprecipitation and real-time PCR. The method reliably enables the detection of sequence-specific DNA binding of full-length p53 at low conce...
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PMID: 20714218
PDF is available here.
PDF is available here.
Abstract
We show that NHR-6 is able to bind the canonical NR4A monomer response element and can transactivate from this site in mammalian HEK293 cells. Using a functional GFP-tagged NHR-6 fusion, we also demonstrate that NHR-6 is nuclear localized during development of the spermatheca. Mutation of the DNA-bi...
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PMID: 20506374
PDF is available here.
PDF is available here.
Abstract
We generated a pancreas-specific ERalpha knockout mouse (PERalpha KO(-/-)) using the Cre-loxP strategy and used a combination of genetic and pharmacologic tools in cultured islets and beta cells. Whereas 17beta-estradiol (E2) treatment up-regulates pancreatic insulin gene and protein content in cont...
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PMID: 20616010
PDF is available here.
PDF is available here.
Brain research (1344) 2010
Abstract
Both over expression of cyclic AMP response element binding protein (CREB) in the nucleus accumbens (NAc), and intra-accumbal injection of cocaine- and amphetamine-regulated transcript (CART) peptides, have been shown to decrease cocaine reward. Also, over expression of CREB in the rat NAc increased...
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PMID: 20451507
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)29 2010
Abstract
We have mapped an antioxidant response element (ARE) in the p62 promoter that is responsible for its induction by oxidative stress via NRF2. Chromatin immunoprecipitation and gel mobility-shift assays verified that NRF2 binds to this cis-element in vivo and in vitro. Also, p62 docks directly onto th...
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PMID: 20452972
PDF is available here.
PDF is available here.
Abstract
We show that the RRE controls the oligomeric state and solubility of Rev and guides its assembly into discrete Rev-RNA complexes. SAXS and EM data were used to derive a structural model of a Rev dimer bound to an essential RRE hairpin and to visualize the complete Rev-RRE RNP, demonstrating that RRE...
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PMID: 20616058
PDF is available here.
PDF is available here.
Abstract
We have isolated a new Ty1-copia-like retrotransposon, named Ttd1a from the Triticum durum L. genome. To get insight into stress activation pathways in Ttd1a, we investigated the effect of salt and light stresses by RT-PCR and S-SAP profiling. We screened for Ttd1a insertion polymorphisms in plants...
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PMID: 20237753
PDF is available here.
PDF is available here.
Abstract
We reported that both wild-type and mutant p53 can also drive transactivation at noncanonical half-site response elements (REs), a finding that greatly expands the universe of genes and regions potentially affected by p53. Furthermore, we found that p53-mediated transcription can be dramatically inc...
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PMID: 20670604
PDF is available here.
PDF is available here.
Abstract
We found that in cultured coronary arterial endothelial cells, resveratrol, in a dose-dependent manner, significantly increases transcriptional activity of Nrf2. Accordingly, resveratrol significantly upregulates the expression of the Nrf2 target genes NAD(P)H:quinone oxidoreductase 1, gamma-glutamy...
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PMID: 20418481
PDF is available here.
PDF is available here.
Abstract
Abstract
We examine the role of these two nuclear factors in the regulation of the expression of human MRP4. HepG2 cells and human hepatocytes were treated with the AhR and Nrf2 activators, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 3-methylcholanthrene (3-MC), or oltipraz and other nuclear receptor agonist...
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PMID: 20395535
PDF is available here.
PDF is available here.
Abstract
We also show that miR-1226 interacts with the MUC1 mRNA 3'UTR and that miR-1226 downregulates endogenous MUC1 protein levels. Consistent with miR-1226-induced downregulation of MUC1 expression, the results demonstrate that miR-1226 induces i) an increase in reactive oxygen species, ii) loss of the m...
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PMID: 20514397
PDF is available here.
PDF is available here.
Endocrinology (151)7 2010
Abstract
We investigated the functional correlation between ERp44 and adiponectin in a pig model. The transcription of porcine ERp44 was regulated by PPARgamma, which was consistent with the finding of putative peroxisome proliferator response element sites within ERp44 promoter. Using chromatin immunoprecip...
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PMID: 20484463
PDF is available here.
PDF is available here.
Abstract
We describe a fast and efficient method for the isolation of the ERalpha receptosome for proteomics analysis. Using immobilized estrogen response element on a Sepharose column in combination with two-dimensional electrophoresis and MALDI-TOF MS, significant amounts of proteins could be isolated and...
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PMID: 20348541
PDF is available here.
PDF is available here.
Molecular Microbiology (77)1 2010
Abstract
We explore the regulation of aox expression and AOX function. Using quantitative PCR and reporter assays, we demonstrate that aox expression is induced in the presence of nitric oxide (NO), and that the NO-responsive regulatory protein NsrR mediates the response. We have identified key amino acid re...
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PMID: 20487270
PDF is available here.
PDF is available here.
Molecular Microbiology (77)1 2010
Abstract
We explore the regulation of aox expression and AOX function. Using quantitative PCR and reporter assays, we demonstrate that aox expression is induced in the presence of nitric oxide (NO), and that the NO-responsive regulatory protein NsrR mediates the response. We have identified key amino acid re...
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PMID: 20487270
PDF is available here.
PDF is available here.
Molecular Microbiology (77)1 2010
Abstract
We explore the regulation of aox expression and AOX function. Using quantitative PCR and reporter assays, we demonstrate that aox expression is induced in the presence of nitric oxide (NO), and that the NO-responsive regulatory protein NsrR mediates the response. We have identified key amino acid re...
|
PMID: 20487270
PDF is available here.
PDF is available here.
Molecular Microbiology (77)1 2010
Abstract
We explore the regulation of aox expression and AOX function. Using quantitative PCR and reporter assays, we demonstrate that aox expression is induced in the presence of nitric oxide (NO), and that the NO-responsive regulatory protein NsrR mediates the response. We have identified key amino acid re...
|
PMID: 20487270
PDF is available here.
PDF is available here.
Molecular Microbiology (77)1 2010
Abstract
We explore the regulation of aox expression and AOX function. Using quantitative PCR and reporter assays, we demonstrate that aox expression is induced in the presence of nitric oxide (NO), and that the NO-responsive regulatory protein NsrR mediates the response. We have identified key amino acid re...
|
PMID: 20487270
PDF is available here.
PDF is available here.
Molecular Microbiology (77)1 2010
Abstract
We explore the regulation of aox expression and AOX function. Using quantitative PCR and reporter assays, we demonstrate that aox expression is induced in the presence of nitric oxide (NO), and that the NO-responsive regulatory protein NsrR mediates the response. We have identified key amino acid re...
|
PMID: 20487270
PDF is available here.
PDF is available here.
Molecular Microbiology (77)1 2010
Abstract
We explore the regulation of aox expression and AOX function. Using quantitative PCR and reporter assays, we demonstrate that aox expression is induced in the presence of nitric oxide (NO), and that the NO-responsive regulatory protein NsrR mediates the response. We have identified key amino acid re...
|
PMID: 20487270
PDF is available here.
PDF is available here.
Molecular Microbiology (77)1 2010
Abstract
We explore the regulation of aox expression and AOX function. Using quantitative PCR and reporter assays, we demonstrate that aox expression is induced in the presence of nitric oxide (NO), and that the NO-responsive regulatory protein NsrR mediates the response. We have identified key amino acid re...
|
PMID: 20487270
PDF is available here.
PDF is available here.
Molecular Microbiology (77)1 2010
Abstract
We explore the regulation of aox expression and AOX function. Using quantitative PCR and reporter assays, we demonstrate that aox expression is induced in the presence of nitric oxide (NO), and that the NO-responsive regulatory protein NsrR mediates the response. We have identified key amino acid re...
|
PMID: 20487270
PDF is available here.
PDF is available here.
Molecular Microbiology (77)1 2010
Abstract
We explore the regulation of aox expression and AOX function. Using quantitative PCR and reporter assays, we demonstrate that aox expression is induced in the presence of nitric oxide (NO), and that the NO-responsive regulatory protein NsrR mediates the response. We have identified key amino acid re...
|
PMID: 20487270
PDF is available here.
PDF is available here.
Molecular Microbiology (77)1 2010
Abstract
We explore the regulation of aox expression and AOX function. Using quantitative PCR and reporter assays, we demonstrate that aox expression is induced in the presence of nitric oxide (NO), and that the NO-responsive regulatory protein NsrR mediates the response. We have identified key amino acid re...
|
PMID: 20487270
PDF is available here.
PDF is available here.
Molecular Microbiology (77)1 2010
Abstract
We explore the regulation of aox expression and AOX function. Using quantitative PCR and reporter assays, we demonstrate that aox expression is induced in the presence of nitric oxide (NO), and that the NO-responsive regulatory protein NsrR mediates the response. We have identified key amino acid re...
|
PMID: 20487270
PDF is available here.
PDF is available here.
Molecular Microbiology (77)1 2010
Abstract
We explore the regulation of aox expression and AOX function. Using quantitative PCR and reporter assays, we demonstrate that aox expression is induced in the presence of nitric oxide (NO), and that the NO-responsive regulatory protein NsrR mediates the response. We have identified key amino acid re...
|
PMID: 20487270
PDF is available here.
PDF is available here.
Molecular Microbiology (77)1 2010
Abstract
We explore the regulation of aox expression and AOX function. Using quantitative PCR and reporter assays, we demonstrate that aox expression is induced in the presence of nitric oxide (NO), and that the NO-responsive regulatory protein NsrR mediates the response. We have identified key amino acid re...
|
PMID: 20487270
PDF is available here.
PDF is available here.
Molecular Microbiology (77)1 2010
Abstract
We explore the regulation of aox expression and AOX function. Using quantitative PCR and reporter assays, we demonstrate that aox expression is induced in the presence of nitric oxide (NO), and that the NO-responsive regulatory protein NsrR mediates the response. We have identified key amino acid re...
|
PMID: 20487270
PDF is available here.
PDF is available here.
Molecular Microbiology (77)1 2010
Abstract
We explore the regulation of aox expression and AOX function. Using quantitative PCR and reporter assays, we demonstrate that aox expression is induced in the presence of nitric oxide (NO), and that the NO-responsive regulatory protein NsrR mediates the response. We have identified key amino acid re...
|
PMID: 20487270
PDF is available here.
PDF is available here.
Molecular Microbiology (77)1 2010
Abstract
We explore the regulation of aox expression and AOX function. Using quantitative PCR and reporter assays, we demonstrate that aox expression is induced in the presence of nitric oxide (NO), and that the NO-responsive regulatory protein NsrR mediates the response. We have identified key amino acid re...
|
PMID: 20487270
PDF is available here.
PDF is available here.