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Osmolar Concentration (2):
Recent article
Osmolar Concentration
Abstract
The heat of lysozyme adsorption on mesostructured cellular foam (MCF) silica was measured using flow microcalorimetry (FMC) to investigate the influence of a neutral salt, sodium sulfate. At concentrations up to 0.5 M sodium sulfate, a complex initial exotherm was followed by an endotherm. Protein su...
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PMID: 21835415
PDF is available here.
PDF is available here.
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Saudi journal of kidney diseases and transplantation : an official publication of the Saudi Center for Organ Transplantation, Saudi Arabia (22)5 2011
Abstract
We have developed since 1994 a new hemodialysis (HD) technique called acetate biofiltration 84% (AFB 84%) that is characterized by the absence of acetate in the dialysate and the complete correction of buffer balance by post- dilutional infusion of bicarbonate- based replacement solution. Our study...
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PMID: 21912029
PDF is available here.
PDF is available here.
Abstract
We review the current knowledge on the molecular stress adaptation responses in L. monocytogenes cells as revealed through transcriptome, proteome, genetic, and physiological analysis. The adaptation of L. monocytogenes to stress exposure is achieved through global expression changes in a large numb...
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PMID: 21495855
PDF is available here.
PDF is available here.
Abstract
We investigated the function and calcium regulation of the COOH-terminal end segment of TnI. A TnI model molecule was labeled with Alexa fluor 532 at a Cys engineered at the COOH-terminal end and used to reconstitute tertiary troponin complex. A Ca(2+) -regulated conformational change in the COOH te...
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PMID: 21777381
PDF is available here.
PDF is available here.
Abstract
We combined methods of computer simulation and experiments based on circular dichroism (CD) to investigate the structural characteristics of CaM that influence its target recognition in crowded cell-like conditions. We developed a unique multiscale solution of charges computed from quantum chemistry...
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PMID: 21829336
PDF is available here.
PDF is available here.
Abstract
Patients exhibited greater fatigue, reduced cardiorespiratory function, higher Medical Research Council (MRC) scores and higher plasma TNF-α concentrations than controls at all times. Plasma IL-1β levels did not differ between cohorts. Patients exhibited a 28% increase (statistically not signif...
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PMID: 21679351
PDF is available here.
PDF is available here.
Abstract
Abstract
Objectives Sulfation via sulfotransferases is an important metabolic pathway contributing to the low bioavailability of flavonoids. This study aims to characterize the sulfation of mono-hydroxyflavones (MHFs) to obtain useful information on structure-metabolizing relationships in animal species an...
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PMID: 21635263
PDF is available here.
PDF is available here.
Abstract
Pediatric Annals (40)6 2011
Abstract
Abstract
We electronically measured the mean cell volume of rat glioma cells under three different experimental conditions, namely: at different osmolarities and constant NaCl concentration; at different NaCl concentrations and constant osmolarity and at different osmolarities caused by changes in NaCl conce...
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PMID: 21613671
PDF is available here.
PDF is available here.
Abstract
We have investigated the effect of adsorbed semi-hydrophobic nanoparticles (NPs) on the dynamics and morphology of model cell membrane. We have systematically varied the degree of surface hydrophobicity of carboxyl end-functionalized polystyrene NPs of varied size in buffer solutions with varied ion...
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PMID: 21631111
PDF is available here.
PDF is available here.
Abstract
The spontaneous folding of two Neisseria outer membrane proteins, opacity-associated (Opa)(60) and Opa(50) into lipid vesicles was investigated by systematically varying bulk and membrane properties. Centrifugal fractionation coupled with sodium dodecyl sulfate polyacrylamide gel electrophoresis mobi...
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PMID: 21539780
PDF is available here.
PDF is available here.
Abstract
I and avoids use of proteases or detergents that degrade the ECM. Characterization of the decellularized muscles using hematoxylin and eosin staining along with DNA quantification suggested complete removal of DNA, whereas biochemical analyses indicated no loss of collagens and only a slight reducti...
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PMID: 20973753
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1810)4 2011
Abstract
We evaluated the mechanism of hypertonicity-induced TNF-α release from activated human monocytic THP-1 cells....
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PMID: 21256190
PDF is available here.
PDF is available here.
Abstract
This investigation provides a simple quantitative and graphical tool that can aid in determining the probability that a person has become dehydrated when serial measures of P(osm), U(sg), or B(m) are made....
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PMID: 21428854
PDF is available here.
PDF is available here.
Abstract
The objective of this study was to review loss of response and need for adalimumab dose intensification in adult and pediatric patients with Crohn's disease. Studies were identified through the electronic databases of MEDLINE and the annual meetings of Digestive Disease Week, of the United European G...
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PMID: 21407178
PDF is available here.
PDF is available here.
Abstract
The Consortium for Radiologic Imaging Studies of Polycystic Kidney Disease (CRISP) was created to identify markers of disease progression in patients with autosomal dominant polycystic kidney disease (ADPKD).
Linear mixed models were utilized to model effects of basel...
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PMID: 21088290
PDF is available here.
PDF is available here.
Abstract
The Consortium for Radiologic Imaging Studies of Polycystic Kidney Disease (CRISP) was created to identify markers of disease progression in patients with autosomal dominant polycystic kidney disease (ADPKD).
Linear mixed models were utilized to model effects of basel...
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PMID: 21088290
PDF is available here.
PDF is available here.
Abstract
The Consortium for Radiologic Imaging Studies of Polycystic Kidney Disease (CRISP) was created to identify markers of disease progression in patients with autosomal dominant polycystic kidney disease (ADPKD).
Linear mixed models were utilized to model effects of basel...
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PMID: 21088290
PDF is available here.
PDF is available here.
Abstract
The Consortium for Radiologic Imaging Studies of Polycystic Kidney Disease (CRISP) was created to identify markers of disease progression in patients with autosomal dominant polycystic kidney disease (ADPKD).
Linear mixed models were utilized to model effects of basel...
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PMID: 21088290
PDF is available here.
PDF is available here.
Abstract
The Consortium for Radiologic Imaging Studies of Polycystic Kidney Disease (CRISP) was created to identify markers of disease progression in patients with autosomal dominant polycystic kidney disease (ADPKD).
Linear mixed models were utilized to model effects of basel...
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PMID: 21088290
PDF is available here.
PDF is available here.
Abstract
We report a case of voluntary poisoning after the patient swallowed antifreeze. Successful treatment began early, with monitoring of the osmolar gap and plasma level correspondence. We also comment on the range of therapeutic options, emphasizing antidotes such as ethanol and fomepizole which provid...
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PMID: 21534295
PDF is available here.
PDF is available here.
Abstract
These results suggest a role for Ca(2+)-activated K(+) channels in human articular chondrocytes, leading to hyperpolarization as a consequence of K(+) efflux through these channels. These channels could have a role in the articular chondrocyte's response to a hyperosmotic challenge and matrix metabo...
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PMID: 21460408
PDF is available here.
PDF is available here.
Specificity of the volume-activated amino acid efflux pathway in cultured human breast cancer cells.
Abstract
It has been shown that cell swelling stimulates the efflux of taurine from MCF-7 and MDA-MB-231 cells via a pathway which has channel-like properties. The purpose of this study was to examine the specificity of the volume-activated taurine efflux pathway in both cell lines. A hyposmotic shock increas...
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PMID: 21460411
PDF is available here.
PDF is available here.
Abstract
The solute rejection versus concentration behaviour of five different amino acids has been investigated using a Nitto Denko NTR7450 nanofiltration membrane. The experimental data for amino acid rejection was also compared against a combined steric and charge rejection model. At its isoelectric point,...
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PMID: 21470590
PDF is available here.
PDF is available here.
Abstract
To evaluate the use of tear osmolarity in the diagnosis of dry eye disease.
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PMID: 21310379
PDF is available here.
PDF is available here.
Abstract
Exercise-induced anaphylaxis (EIA) and food-dependent, exercise-induced anaphylaxis (FDEIA) are rare but potentially life-threatening clinical syndromes in which association with exercise is crucial. The range of triggering physical activities is broad, including as mild an effort as a stroll. EIA is...
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PMID: 20922508
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1814)3 2011
Abstract
We compared the proteome of a) the hyperosmotic renal papilla with that of adjacent iso-osmotic cortex tissue and b) the renal papilla of diuretic versus that of anti-diuretic mice. Though functionally distinct the papilla is in close physical proximity to the renal cortex, an iso-osmotic region. Pr...
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PMID: 21236367
PDF is available here.
PDF is available here.
Abstract
Most proteins and large polypeptides have hydrophobic regions at their surface. These hydrophobic "patches" are due to the presence of the side chains of hydrophobic or nonpolar amino acids such as phenylalanine, tryptophan, alanine, and methionine. These surface hydrophobic regions are interspersed...
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PMID: 20978980
PDF is available here.
PDF is available here.
Abstract
We review the basic principles of IEC, as well as the broader criteria for selecting IEC conditions. By way of further illustration, we outline protocols necessary to partially purify a serine peptidase from bovine whole brain cytosolic fraction, covering crude tissue extract preparation through to...
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PMID: 20978968
PDF is available here.
PDF is available here.
Abstract
Abstract
Consumption of meals with different macronutrient contents, especially high in carbohydrates, may influence the stress-induced physiological and psychological response. The objective of this study was to investigate effects of consumption of a high-protein vs. high-carbohydrate meal on the physiologi...
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PMID: 21304815
PDF is available here.
PDF is available here.
Abstract
We had observations of the peristalsis of the renal pelvis surrounding the papilla. The urine concentration falls if the peristalsis is stopped. I was wrong; together these lines of evidence show that the renal papilla is not just an elongation of the inner medulla. We are left without a full explan...
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PMID: 21281458
PDF is available here.
PDF is available here.
Abstract
We report the case of a 82 year-old woman with hyperosmolar hyperglycemic state (HHS) associated with hypernatremia, who was treated using 0.2 % sodium chloride in 5 % dextrose in water which resulted in the amelioration of the neurological symptoms in a short period of time, followed the decrease i...
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PMID: 21839004
PDF is available here.
PDF is available here.
Abstract
Changes in the rigidity of the polymetric chain of phage lambda double-strand DNA have been studied by laser correlation spectroscopy. It was shown that, as the ionic strength increases, the effect of the screening of the hydrodynamic interaction of the links of the polymeric chain specific for polym...
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PMID: 21950067
PDF is available here.
PDF is available here.
Abstract
We found that Japanese refractometers overestimated USG in both dogs and cats. This study shows that the correlation formulas described in this study are useful for the accurate evaluation of USG....
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PMID: 21187683
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1807)6 2011
Abstract
We identified control coefficients with fixed signs in order to predict the pattern of changes in key enzyme activities which can explain the observed changes in fluxes and concentrations underlying the metabolic adaptations in oncogenic K-ras transformation in NIH-3T3 cells. The fixed signs of cont...
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PMID: 21185256
PDF is available here.
PDF is available here.
Abstract
Our objective was to describe cases of hyperosmolar hyperglycemic state (HHS) in advanced amyotrophic lateral sclerosis (ALS) patients and discuss its pathophysiology. Five ventilator-dependent patients with ALS, with no previous history of diabetes, showed development of marked hyperglycemia (plasm...
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PMID: 21126160
PDF is available here.
PDF is available here.
Abstract
We examined the effects of ASL volume expansion on ENaC activity in primary human bronchial epithelial (HBE) cell cultures. We found that ENaC activity increases dramatically after rapid dilution of endogenous ASL. Approximately 35% of the increase in Na(+) absorption was attributable to activation...
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PMID: 20097829
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1807)6 2011
Abstract
Most cancer cells exhibit an accelerated glycolysis rate compared to normal cells. This metabolic change is associated with the over-expression of all the pathway enzymes and transporters (as induced by HIF-1α and other oncogenes), and with the expression of hexokinase (HK) and phosphofructokinase t...
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PMID: 21110941
PDF is available here.
PDF is available here.
Revue medicale suisse (6)269 2010
Abstract
We summerize its diagnostic approach based on the physiopathology. Assessment of volume status, measurement of plasma and urinary osmolality remain key steps in the management of this electrolyte disorder....
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PMID: 21140961
PDF is available here.
PDF is available here.
Biochemistry (Washington) (49)43 2010
Abstract
The prevention of aggregation in therapeutic antibodies is of great importance to the biopharmaceutical industry. In our investigation, acid-induced aggregation of monoclonal IgG1 and IgG2 antibodies was studied at pH 3.5 as a function of salt concentration and buffer type. The extent of aggregation...
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PMID: 20843079
PDF is available here.
PDF is available here.
Journal of Chromatography A (1217)43 2010
Abstract
A simple and fast sample preparation method for the determination of nonylphenol (NP) and octylphenol (OP) in aqueous samples by simultaneous derivatization and dispersive liquid-liquid microextraction (DLLME) was investigated using gas chromatography-mass spectrometry (GC/MS). In this method, a com...
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PMID: 20615510
PDF is available here.
PDF is available here.
Abstract
A fluorescent probe for mercury(II) ions, based on the quenching of fluorescence of terbium(III) ions doped in CdS nanoparticles, has been developed. The terbium(III)-doped cadmium sulfide composite nanoparticles were successfully synthesized through a straightforward one-pot process, with the biomo...
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PMID: 20663708
PDF is available here.
PDF is available here.
Abstract
The interaction between photosensitizer anticancer drug hematoporphyrin monomethyl ether (HMME) and ctDNA has been studied based on the decreased resonance light scattering (RLS) phenomenon. The RLS, UV-vis and fluorescence spectra characteristics of the HMME-ctDNA system were investigated. Besides,...
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PMID: 20643575
PDF is available here.
PDF is available here.
Abstract
We evaluated the possible routes of the β-CN (193-209) peptide transport, using selective inhibitors of the different routes for peptide transfer through the intestinal barrier. The results showed that the β-CN (193-209) peptide resisted the action of brush-border membrane peptidases, and that it...
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PMID: 20397193
PDF is available here.
PDF is available here.
Abstract
We evaluated the incidence of CI-AKI, in-hospital dialysis and in-hospital death in 58,957 patients who underwent PCI in 2007 and 2008 in a large regional consortium of 31 hospitals and who were treated with iodixanol (n = 17,814) or LOCM (n = 41,143). Propensity-matched analysis was performed to ad...
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PMID: 20944185
PDF is available here.
PDF is available here.
Bioresource Technology (101)19 2010
Abstract
For the sensitive determination of Pb(II) from aqueous solutions, a new voltammetric biosensor based on carbon paste electrode modified with Rhizopus arrhizus was developed. The preconcentrated ions at open circuit were reduced by using differential pulse stripping voltammetry techni...
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PMID: 20576569
PDF is available here.
PDF is available here.
Abstract
To investigate the effects of medium osmolality on the expansion of human articular chondrocytes (HACs) with serum-free media (SFM), proprietary SFM of various osmolalities (290, 320, 350, 400, and 450 mOsm/kg), supplemented with components known to enhance chondrocyte growth, were constructed by th...
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PMID: 20001269
PDF is available here.
PDF is available here.
Abstract
Tear film osmolarity measured with the OcuSense TearLab system correlates well with the Clifton Osmometer. The new instrument has the potential to provide clinicians with a readily available clinically applicable measure, which could become the gold standard in DED....
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PMID: 20577088
PDF is available here.
PDF is available here.
Meat Science (86)1 2010
Abstract
As myofibrils consist of a three-dimensional network of long, solid protein particles with the shortest dimension of less than 20 nm, the theoretical foundations of water-holding in meat should be studied from a colloid or surface chemistry point of view. The classical hypotheses for water-holding i...
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PMID: 20627421
PDF is available here.
PDF is available here.
Abstract
To better address the need for developing stable formulations of plasmid DNA-based biopharmaceuticals, 37 compounds from a generally regarded as safe library were examined for their potential use as stabilizers. A plasmid DNA-based therapeutic vaccine, BHT-DNA, was used as a model system. Initial stu...
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PMID: 20740679
PDF is available here.
PDF is available here.
Abstract
The influence of confinement on the persistence length of dsDNA molecules under a high ionic strength environment was explored by coarse-grained Monte Carlo simulations in channels of different profiles. It was found that under confinement three definitions of the persistence length of DNA molecules...
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PMID: 20589298
PDF is available here.
PDF is available here.
Abstract
Our results indicate that the OpdA Tyr(607) residue plays an important role in the enzyme-substrate interaction and in the hydrolytic activity.
2010 Elsevier Inc. All rights reserved....
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PMID: 20513640
PDF is available here.
PDF is available here.
Animal Science Journal (81)4 2010
Abstract
These results showed that the high osmolarity of a culture medium induced by increasing NaCl concentration during the first 2 days improves the development of pig oocytes and miniature pig SCNT embryos activated by ultrasound....
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PMID: 20662814
PDF is available here.
PDF is available here.
Hematology (15)4 2010
Abstract
We used a specific anti-miR-21 oligonucleotide (AMO-miR-21) to sensitize leukemic HL60 cells to arabinosylcytosine (Ara-C) by down-regulating miR-21. AMO-miR-21 alone effectively inhibited HL60 cell viability as measured by MTT assays and induced apoptosis as evaluated by flow cytometry, whereas AMO...
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PMID: 20670480
PDF is available here.
PDF is available here.
Abstract
We show that resveratrol inhibited cholera toxin (CT)-induced cyclic AMP accumulation in Vero cells. Resveratrol suppressed the CT activity by suppressing the internalization of CT and traffic to the Golgi apparatus without affecting CT binding to cells. Further, resveratrol partially precipitated C...
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PMID: 20307562
PDF is available here.
PDF is available here.
Abstract
We report six new three-finger like toxins isolated from the venom of the coral snake M. frontalis which we named Frontoxin (FTx) I-VI. Toxins were purified using multiple steps of RP-HPLC. Molecular masses were determined by MALDI-TOF and ESI ion-trap mass spectrometry. The complete amino acid sequ...
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PMID: 20331995
PDF is available here.
PDF is available here.
Abstract
We recommend that the practice of administering repeated dose of mannitol for prophylactic purposes be reevaluated....
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PMID: 20804114
PDF is available here.
PDF is available here.