Abstract
The main methods for the simplification of the NMR of complex mixtures by selective attenuation/suppression of the signals of certain components are presented. The application of relaxation, diffusion and PSR filters and other techniques to biological samples, pharmaceuticals, foods, living organism...
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PMID: 20936238
PDF is available here.
Abstract
This review will detail the motivations, experimental approaches, and growing list of successful cases associated with the heterologous production of complex natural products.
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PMID: 21060956
PDF is available here.
Abstract
Because yeast is exceptionally well suited to genetic analysis, both classical and molecular, it is an attractive system for expressing recombinant animal proteins for purification purposes. Methods available for lysing yeast cells include autolysis, pressure cells (e.g., French pres...
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PMID: 21205845
PDF is available here.
Abstract
Astrocytes are often referred to, and historically have been regarded as, support cells of the mammalian CNS. Work over the last decade suggests otherwise-that astrocytes may in fact play a more active role in higher neural processing than previously recognized. Because astrocytes can potentially se...
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PMID: 20880499
PDF is available here.
Abstract
Although matrix-assisted laser desorption and ionization (MALDI) mass spectrometry (MS)--often but not exclusively coupled with a time-of-flight (TOF) mass analyzer--is primarily established in the protein field, there is increasing evidence that MALDI MS is also very useful in lipid research: MALDI...
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PMID: 20643161
PDF is available here.
Abstract
Human skeletal stem cells (STRO-1 positive/STRO-1+) respond to different topographical features in various ways. On a flat surface these cells spread and tend to develop a fibroblast-like morphology. On a microgrooved surface enriched skeletal stem cell populations prefer to stretch...
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PMID: 20302977
PDF is available here.
Abstract
Comparing breakthrough cures of five starch-based materials experimentally prepared for ethanol dehydration, a compound adsorptive agent ZSG-1 was formulated with high adsorption capacity, low energy and material cost. The selective water adsorption was conducted in a fixed-bed absorber packed with...
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PMID: 20304634
PDF is available here.
Abstract
Frondanol-A5 causes cell cycle arrest and apoptosis in human pancreatic cancer cells. These changes are associated with decreased expression of cyclin A, cyclin B, and cdc25c and increased expression of p21 that, at least in part, is mediated by a p38 kinase-dependent mechanism. Because Frondanol-A5...
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PMID: 20124937
PDF is available here.
Abstract
A hydraulic coagulation-flocculation processes combined with aerated spiral-wound ultrafiltration membranes (ASWUF) was designed with the objective of improving natural organic matter (NOM) removal by ASWUF in the treatment of water for human consumption. The pilot-scale experimental system had capa...
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PMID: 20153578
PDF is available here.
Abstract
ECD was 2726 +/- 419 cells/mm(2) in the enucleation and 2645 +/- 395 cells/mm(2) in the in situ group (P < .001). D-to-P was 9.81 +/- 3.56 hours and 8.90 +/- 3.65 hours, respectively (P < .001). The mean grade of the stroma was 1.44 +/- 0.51 in the enucleation group and 1.50 +/- 0.56 in the in situ...
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PMID: 20570238
PDF is available here.
Abstract
This technical note presents a simple and disposable lab-on-a-tube (LOT) for point-of-care measurements of multiple analytes. LOT is a one-step device that can perform both sample collection and multi-sensing on-site. Sample collection is conducted by taking advantage of its inherent micro/macro cha...
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PMID: 20480114
PDF is available here.
Abstract
We create a platform where only elongational viscoelastic instabilities, and diffusion, affect mixing. In contrast to earlier studies with significant shear that found up to orders of magnitude increase in mixing we find that inclusion of polymers excites large viscoelastic instabilities yet mixing...
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PMID: 20480108
PDF is available here.
Roberto Christ Vianna Santos,
Adroaldo Lunardelli,
Eduardo Caberlon,
Carolina Maria Alves Bastos,
Fernanda Bordignon Nunes,
Melissa Guerra Simões Pires,
Vanderlei Biolchi,
Elisiane Lozza Paul,
Francesco Breno Campolongo Vieira,
Alzira Resende do Carmo Aquino,
Elio Corseuil and
Jarbas Rodrigues de Oliveira
Abstract
We used an acute inflammation model of injury, injection of carrageenan into the pleural cavity of rats. The rats were treated intraperitoneally with the aqueous extract of U. dermestoides 8 and 16 mg/kg. The exudate volume, protein concentration, polymorphonuclear leukocytes (PMNs) and total leukoc...
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PMID: 20020191
PDF is available here.
Abstract
The adsorption of two basic dyes, CI Basic Yellow 28 and CI Basic Green 4, was studied in single and binary solute systems using two classes of inorganic-organic pillared clay granules as sorbents (300-400 microm and 700-800 microm). These were prepared by high-shear wet granulation from an Al cetyl...
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PMID: 20586243
PDF is available here.
Abstract
Knowledge about the protein targets of therapeutic agents is critical for understanding drug mode of action. Described here is a mass spectrometry-based proteomics method for identifying the protein target(s) of drug molecules that is potentially applicable to any drug compound. The method, which in...
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PMID: 20439767
PDF is available here.
Abstract
Rates for local and systemic reactions that might possibly be related to the study medication were 2.36 and 4.56/1000 injections, respectively. With 5923 injections given, 14 local reactions were reported (5 patients), and 27 systemic reactions were reported, including 20 of grade 1 (6 patients) and...
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PMID: 20167138
PDF is available here.
Abstract
We develop a method to separate the sources through minimizing a p-norm-like diversity measure under some mild assumptions which are generally valid for EPR signals. Simulations demonstrate that the proposed method performs well on EPR signal separation, with better robustness to noise compared to o...
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PMID: 20194039
PDF is available here.
Abstract
The main objective of the present study was to investigate the efficiency of titanium dioxide (TiO2) assisted photocatalytic degradation (PCD) process for the removal of ammonium-ammonia (NH4(+)-NH3) from the aqueous phase and in the presence of co-pollutants thiosulfate (S2O3(2-)) and p-cresol (C6H...
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PMID: 20540426
PDF is available here.
Abstract
Surface-enhanced Raman spectroscopy (SERS) allows a new insight into the analysis of cell physiology. In this work, the difficulty of producing suitable substrates that, besides permitting the amplification of the Raman signal, do not interact with the biological material causing alteration, has bee...
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PMID: 20348602
PDF is available here.
Abstract
To investigate whether human limbal epithelial cells (HLEC) that have been cultured on intact or de-epithelialized amniotic membranes (AMs) demonstrate differences in morphology after 1 week of eye bank storage.
HLEC were cultured from limbal explants for 3 weeks on intact AM and AM deprived of the...
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PMID: 20164752
PDF is available here.
Abstract
After short term exposure increased secretion of all investigated steroids was noted only under the influence of small dose of mixture. However, after longer time of exposure the stimulation of estradiol and testosterone secretion was observed under the influence of both the low and medium dose of m...
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PMID: 20429633
PDF is available here.
Abstract
Cilia were not measured quantitatively but were equally present on the treated and untreated mucosa. There was no histological evidence of inflammation, epithelial injury, or significant morphological changes. CONCLUSION: The application of a manuka honey solution to rabbit nasal respiratory mucosa...
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PMID: 20338104
PDF is available here.
Abstract
House dust mite is a major cause of allergic asthma and rhinitis in Thai population. Skin prick test (SPT) is a useful tool for the diagnosis of the IgE-mediated reactions. The imported commercial mite vaccine for SPT is available but it is relatively expensive. Aim of this study is to compare Sirir...
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PMID: 20527515
PDF is available here.
Abstract
The purpose of this study was to determine whether a mineral-rich extract derived from the red marine algae Lithothamnion calcareum could be used as a dietary supplement for chemoprevention against colon polyp formation. A total of 60 C57bl/6 mice were divided into 3 groups based on...
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PMID: 20150219
PDF is available here.
Abstract
We conclude that ME and FBE glucans may exert a direct antiproliferative effect on cancer cells expressing high galectin-3 concentrations and concomitantly downregulate tumor cell adherence, the latter being directly related to cancer progression and metastasis....
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PMID: 19830415
PDF is available here.
Abstract
We need to explore biology-based models. In this paper, we present an integrated approach to deal with the toxic effects of mixtures on growth, reproduction and survival, over the life cycle. Toxicokinetics is addressed with a one-compartment model, accounting for effects of growth. Each component o...
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PMID: 19771510
PDF is available here.
Abstract
We need to explore biology-based models. In this paper, we present an integrated approach to deal with the toxic effects of mixtures on growth, reproduction and survival, over the life cycle. Toxicokinetics is addressed with a one-compartment model, accounting for effects of growth. Each component o...
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PMID: 19771510
PDF is available here.
Abstract
We need to explore biology-based models. In this paper, we present an integrated approach to deal with the toxic effects of mixtures on growth, reproduction and survival, over the life cycle. Toxicokinetics is addressed with a one-compartment model, accounting for effects of growth. Each component o...
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PMID: 19771510
PDF is available here.
Abstract
We need to explore biology-based models. In this paper, we present an integrated approach to deal with the toxic effects of mixtures on growth, reproduction and survival, over the life cycle. Toxicokinetics is addressed with a one-compartment model, accounting for effects of growth. Each component o...
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PMID: 19771510
PDF is available here.
Abstract
We need to explore biology-based models. In this paper, we present an integrated approach to deal with the toxic effects of mixtures on growth, reproduction and survival, over the life cycle. Toxicokinetics is addressed with a one-compartment model, accounting for effects of growth. Each component o...
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PMID: 19771510
PDF is available here.
Abstract
We need to explore biology-based models. In this paper, we present an integrated approach to deal with the toxic effects of mixtures on growth, reproduction and survival, over the life cycle. Toxicokinetics is addressed with a one-compartment model, accounting for effects of growth. Each component o...
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PMID: 19771510
PDF is available here.
Abstract
We need to explore biology-based models. In this paper, we present an integrated approach to deal with the toxic effects of mixtures on growth, reproduction and survival, over the life cycle. Toxicokinetics is addressed with a one-compartment model, accounting for effects of growth. Each component o...
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PMID: 19771510
PDF is available here.
Abstract
We need to explore biology-based models. In this paper, we present an integrated approach to deal with the toxic effects of mixtures on growth, reproduction and survival, over the life cycle. Toxicokinetics is addressed with a one-compartment model, accounting for effects of growth. Each component o...
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PMID: 19771510
PDF is available here.
Abstract
We need to explore biology-based models. In this paper, we present an integrated approach to deal with the toxic effects of mixtures on growth, reproduction and survival, over the life cycle. Toxicokinetics is addressed with a one-compartment model, accounting for effects of growth. Each component o...
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PMID: 19771510
PDF is available here.
Abstract
We need to explore biology-based models. In this paper, we present an integrated approach to deal with the toxic effects of mixtures on growth, reproduction and survival, over the life cycle. Toxicokinetics is addressed with a one-compartment model, accounting for effects of growth. Each component o...
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PMID: 19771510
PDF is available here.
Abstract
We need to explore biology-based models. In this paper, we present an integrated approach to deal with the toxic effects of mixtures on growth, reproduction and survival, over the life cycle. Toxicokinetics is addressed with a one-compartment model, accounting for effects of growth. Each component o...
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PMID: 19771510
PDF is available here.
Abstract
We need to explore biology-based models. In this paper, we present an integrated approach to deal with the toxic effects of mixtures on growth, reproduction and survival, over the life cycle. Toxicokinetics is addressed with a one-compartment model, accounting for effects of growth. Each component o...
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PMID: 19771510
PDF is available here.
Abstract
There was investigated the influence of zinc complex with enzymatic hydrolyzate of mussels' meat added into zinc-deficient diet on a body mass rats, contents in blood alimentary antigen and activity alkalis phosphatase The results obtained showed a good advance of mussels' meat enzymatic hydrolyzate...
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PMID: 20560489
PDF is available here.
Abstract
Investigations with the use of a rat model of adjuvant arthritis have demonstrated long-term immune, biochemical, and anti-inflammatory effects of preparations based on green and brown marine algae from the Anapa region (the Black Sea). Comparative studies showed that the algal preparations are in m...
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PMID: 21086605
PDF is available here.
Abstract
The solubility of chlorhexidine is highly dependent on the salt present in solution. The aim of present research was evaluation of the effect of two polymers: polyacrylic acid and methylcellulose on the pH of resulting preparation, containing chlorhexidine. Also the solubility aspect was considered...
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PMID: 20446528
PDF is available here.
Abstract
The most important allergen manufacturers are based in Europe and in the US. In some countries local products are also sold. No comparison between European, US and local products has been made until now.
To determine total protein content and total specific IgE binding capacity or major allergen con...
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PMID: 20338683
PDF is available here.
Abstract
Beta-galactosidase of Aspergillus sp. AF crude homogenate was immobilized in Ca-alginate gel beads and used for the production of galactooligosaccharides (GOS) from lactose. Optimum pH and temperature, thermal and storage stability of the enzyme activity were investigated and compared with those of...
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PMID: 20082600
PDF is available here.
Abstract
Inhalation of gases, vapors and aerosols can cause a wide range of adverse health effects, ranging from simple irritation to systemic diseases. The large number of chemicals and complex mixtures present in indoor and outdoor air coupled with the introduction of new materials such as nanoparticles an...
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PMID: 20358692
PDF is available here.
Abstract
We describe an experimental approach that fractionates proteins released from human platelets, linking bioassay activity to identity. We used consecutive orthogonal separation platforms to ensure sensitive detection: (a) ion-exchange of intact proteins, (b) SDS-PAGE separation of ion-exchange fracti...
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PMID: 20368775
PDF is available here.
Abstract
CTP provided a significant (p = 0.01) antiplaque effect versus CRT. The results are consistent with previously reported data for CTT. All three examiners demonstrated a strong correlation for this clinical study utilizing the MGMPI methodology. CONCLUSION: This clinical investigation examined the ef...
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PMID: 20527504
PDF is available here.
Abstract
We report on the direct measurement of critical Casimir forces by total internal reflection microscopy with femtonewton resolution. The corresponding potentials are determined for individual colloidal particles floating above a substrate under the action of the critical thermal noise in the solvent...
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PMID: 20365154
PDF is available here.
Abstract
We attempt to elucidate this phenomenon and propose a qualitative mechanism for the separation of species in binary associating mixtures. A correlation between the sign change in the thermal diffusion factor and a change in the molecular structure, mixture viscosity, and the excess entropy of mixing...
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PMID: 20365155
PDF is available here.
Abstract
We use molecular simulations to investigate whether Rosenfeld's observation is a special case of a more general scaling law relating the tracer diffusivities of particles in mixtures to the excess entropy. Specifically, we study the tracer diffusivities, static structure, and thermodynamic propertie...
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PMID: 20365159
PDF is available here.
Abstract
Clipping a Gaussian random field at a level that is position-dependent yields statistically inhomogeneous morphologies, relevant to many ordered nanostructured materials. The one-point and two-point probability functions of the morphology are derived, as well as a general relation between the specif...
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PMID: 20365169
PDF is available here.
Abstract
We focus on dynamics upon supercooling and in particular on testing the mode coupling theory properties of the confined mixture. Parameters of mode coupling theory in going from bulk to weak confinement, and from weak to strong confinement are extracted from simulations and analyzed. We focus on the...
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PMID: 20365174
PDF is available here.
Abstract
The peptide antibiotic mutacin 1140 belongs to the group of antibiotics called lantibiotics. They are ribosomally synthesized and undergo extensive enzymatic modifications before being excreted into the culture medium. By using reverse-phase-high-performance liquid chromatography (RP-HPLC) and a sem...
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PMID: 19940934
PDF is available here.
Abstract
We unveil the shear-driven aggregation mechanism at the origin of structure formation in sheared colloidal systems. The theory, verified against numerics and experiments, explains the induction time followed by explosive (irreversible) rise of viscosity observed in charge-stabilized colloidal and pr...
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PMID: 20364982
PDF is available here.
Abstract
Self-consistent field theory is applied to model the structure and stability of polyelectrolyte complex micelles with incorporated protein (molten globule) molecules in the core. The electrostatic interactions that drive the micelle formation are mimicked by nearest-neighbor interactions using Flory...
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PMID: 20364984
PDF is available here.
Abstract
We show that for the Boolean model of random composite media one can, from a single image of a system at any particle fraction, define a set of parameters which allows one to accurately reconstruct the medium for all other phase fractions. The morphological characterization is based on a family of m...
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PMID: 20364976
PDF is available here.
Abstract
We here describe a protocol for the discovery of natural products with antimalarial, antileishmanial and antitrypanosomal activity, from extracts libraries in 96-well format. Analytical gradient HPLC on a 3 x 150 mm column of 350 microg of extract, and collection of one-minute fractions into 96 deep...
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PMID: 19911575
PDF is available here.
Abstract
The small-time asymptotic properties of the reaction front formed by a reaction A+B-->C coupled to diffusion are considered. Reactants A and B are initially separately dissolved in two identical solvents. The solvents are brought into contact and the reactants meet through diffusion. The small-time...
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PMID: 19905401
PDF is available here.
Abstract
In the present paper, a one-temperature model of transport properties in chemically nonequilibrium neutral gas-mixture flows with electronic excitation is developed. The closed set of governing equations for the macroscopic parameters taking into account electronic degrees of freedom of both molecul...
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PMID: 19905461
PDF is available here.
Abstract
Recently developed analytic approximation for the equation of state of fully ionized nonideal electron-ion plasma mixtures [A. Y. Potekhin, G. Chabrier, and F. J. Rogers, Phys. Rev. E 79, 016411 (2009)], which covers the transition between the weak and strong Coulomb coupling regimes and reproduces...
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PMID: 19905488
PDF is available here.