Find your PDFs fast.
Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Microscopy, Immunoelectron (0):
Recent article
Microscopy, Immunoelectron
Abstract
We studied the localization, activation and function of protease-activated receptor 1 (PAR-1) at the CNS synapse utilizing rat brain synaptosomes and slices. Confocal immunofluoresence and transmission electron microscopy in brain slices with pre-embedding diaminobenzidine (DAB) immunostaining found...
|
PMID: 21854391
PDF is available here.
PDF is available here.
| < More recent | Older article > |
Abstract
We present a mouse model in which a polyclonal sheep anti-mouse podocyte Ab caused subepithelial immune complex formation. Mice developed a nephrotic syndrome with severe edema, proteinuria, hypoalbuminemia, and elevated cholesterol and triglycerides. Development of proteinuria was biphasic: an init...
|
PMID: 21844386
PDF is available here.
PDF is available here.
Abstract
We thus propose that the association of antigens from dead cells with DS is a possible origin of autoantigens and that autoreactive B-1a cells are positively selected and expanded by DS∙autoantigen complexes. This mechanism may also explain the clonal expansion of B-1a cells in certain B-cell mali...
|
PMID: 21514431
PDF is available here.
PDF is available here.
Abstract
We derived a 22 Å-resolution three-dimensional (3D) density map of LDL using cryo-electron microscopy and image reconstruction, which showed a backbone of high-density regions that encircle the LDL particle. Additional high-density belts complemented this backbone high density to enclose the edge o...
|
PMID: 21460103
PDF is available here.
PDF is available here.
Abstract
We clarified a unique molecular convergence of DGLα, CB(1), and MGL at specific inhibitory synapses in the basal nucleus (BA), but not lateral nucleus, of the basolateral amygdala. The synapses, termed invaginating synapses, consisted of conventional symmetrical contact and unique perisynaptic inva...
|
PMID: 21282604
PDF is available here.
PDF is available here.
Abstract
We performed subcellular fractionation and electron microscopic analysis using brains of normal and scrapie-infected mice. Peptidylarginine deiminase 2 was abundantly present in cytosol and weakly in microsomal and mitochondrial fractions and expression in these fractions was higher in brains of scr...
|
PMID: 21343880
PDF is available here.
PDF is available here.
Abstract
It is becoming clear that the nano/microtopography of a biomaterial in vivo is of first importance in influencing focal adhesion formation and subsequent cellular behaviour. When considering next-generation biomaterials, where the material's ability to elicit a regulated cell response will be key to...
|
PMID: 21042965
PDF is available here.
PDF is available here.
EMBO Journal (30)18 2011
Abstract
We found that ERK7 negatively regulates secretion in response to serum and amino-acid starvation, in both Drosophila and human cells. Under these conditions, ERK7 turnover through the proteasome is inhibited, and the resulting higher levels of this kinase lead to a modification in a site within the...
|
PMID: 21847093
PDF is available here.
PDF is available here.
Abstract
We identified differences in mitochondrial properties and function of synaptic vs. nonsynaptic mitochondrial populations in the transgenic mouse brain, which overexpresses the human mutant form of amyloid precursor protein and Aβ. Compared with nonsynaptic mitochondria, synaptic mitochondria showed...
|
PMID: 20937894
PDF is available here.
PDF is available here.
Histology and histopathology (25)10 2010
Abstract
We observed alpha5, beta1 and gamma3 subunit immunoreactivity in the mouse testes, specifically in the cytoplasm surrounding the nucleus in the spermatocytes and spermatids. In the current study, alpha1 subunit immunoreactivity was located in the nucleus of spermatogonia, spermatocytes and round spe...
|
PMID: 20712007
PDF is available here.
PDF is available here.
Abstract
We confirm here using different morphological and biochemical procedures that ubiquilin is present in autophagosomes in HeLa cells and in brain and liver tissue of mouse. Coimmunoprecipitation studies indicated that ubiquilin binds the autophagosome marker LC3 in a complex and that reduction of ubiq...
|
PMID: 20529957
PDF is available here.
PDF is available here.
Human Molecular Genetics (19)16 2010
Abstract
We confirm here using different morphological and biochemical procedures that ubiquilin is present in autophagosomes in HeLa cells and in brain and liver tissue of mouse. Coimmunoprecipitation studies indicated that ubiquilin binds the autophagosome marker LC3 in a complex and that reduction of ubiq...
|
PMID: 20529957
PDF is available here.
PDF is available here.
Developmental Cell (19)1 2010
Abstract
We demonstrate that in migrating cells, a fraction of the endocytosed fibronectin receptor, alpha 5 beta 1 integrin, is sorted into multivesicular endosomes together with fibronectin and degraded in lysosomes. This sorting requires fibronectin-induced ubiquitination of the alpha 5 subunit, and the a...
|
PMID: 20643357
PDF is available here.
PDF is available here.
Abstract
These results suggest that in addition to its well-known effect on transcription regulation of mnp genes, manganese influences secretion of MnP to the extracellular medium. Experiments carried out in the presence of cycloheximide confirmed that the metal is required to secrete MnP already synthesize...
|
PMID: 20434578
PDF is available here.
PDF is available here.
Infection and Immunity (78)7 2010
Abstract
We have previously shown that pathogenic leptospiral strains are able to bind C4b binding protein (C4BP). Surface-bound C4BP retains its cofactor activity, indicating that acquisition of this complement regulator may contribute to leptospiral serum resistance. In the present study, the abilities of...
|
PMID: 20404075
PDF is available here.
PDF is available here.
Abstract
We developed an immunoelectron microscopic (IEM) detection method using anti-CML monoclonal antibody 6D12 in conjunction with computer-assisted image analysis. Antibody was pre-absorbed with purified AGE-bovine serum albumin to assure specificity. Antigen-antibody (ag-ab) complexes were individually...
|
PMID: 20490536
PDF is available here.
PDF is available here.
Abstract
FRalpha and PCFT are expressed in retinal Müller cells and colocalize in the endosomal compartment, suggesting that the two proteins may work coordinately to mediate folate uptake. The unexpected finding of RFC expression and activity in cultured Müller cells may reflect the upregulation of this p...
|
PMID: 20053979
PDF is available here.
PDF is available here.
Annals of Neurology (67)6 2010
Abstract
We studied CFTR expression in human skeletal muscle by Western blot with anti-CFTR antibody (Ab) L12B4 and demonstrated a single band with expected molecular weight of 168kDa. We isolated the cDNA by reverse transcription polymerase chain reaction and directly sequenced a 975bp segment (c. 3,600-4,5...
|
PMID: 20517942
PDF is available here.
PDF is available here.
Abstract
We have examined the cellular and subcellular localization of Kv1.3 in the medial nucleus of the trapezoid body (MNTB) in the auditory brainstem, a nucleus in which neurons fire at high rates with high temporal precision. A clear gradient of Kv1.3 immunostaining along the lateral to medial tonotopic...
|
PMID: 20575068
PDF is available here.
PDF is available here.
Abstract
We found intense staining in the piriform cortex, amygdala, hypothalamus (medial preoptic area, supra chiasmatic nucleus, lateral hypothalamus (LH), ventromedial hypothalamic nucleus, and the arcuate nucleus, habenular nuclei, laterodorsal tegmental nucleus (LDTg), pedunculopontine tegmental nucleus...
|
PMID: 20331985
PDF is available here.
PDF is available here.
Abstract
We attempted to reconstitute disrupted BBPP and blood vessels using skeletal muscle-derived multipotent stem cells that show synchronized reconstitution capacity of vascular, muscular, and peripheral nervous systems. METHODS.: Under pentobarbital anesthesia, intravesical pressure by electrical stimu...
|
PMID: 20150836
PDF is available here.
PDF is available here.
Human Vaccines (6)5 2010
Abstract
We demonstrate that truncation of the nuclear targeting and DNA binding signals at the C-terminus of the HPV-16 and HPV-18 L1 proteins prevented intranuclear formation of the VLPs in the host cells and led to cytoplasmic localization of the L1 proteins as shown by in situ immunogold detection and el...
|
PMID: 20953154
PDF is available here.
PDF is available here.
Abstract
Renal dysfunction is often seen in patients with plasma cell dyscrasias. The abnormal light and heavy chains that are produced by the neoplastic plasma cells in these patients are responsible for the renal abnormalities that occur. The renal manifestations are heterogeneous and include alterations i...
|
PMID: 20455664
PDF is available here.
PDF is available here.
Abstract
We report the intracellular distribution of a novel L. donovani secretory serine protease in the flagellar pocket, as determined by immunogold labeling. Flow cytometry and confocal immunofluorescence analysis revealed that the expression of the protease diminishes sequentially from virulent to atten...
|
PMID: 20106998
PDF is available here.
PDF is available here.
Abstract
We report marvelD3, a novel tight junction protein that, like occludin and tricellulin, contains a conserved four-transmembrane MARVEL (MAL and related proteins for vesicle trafficking and membrane link) domain. Phylogenetic tree reconstruction; analysis of RNA and protein tissue distribution; immun...
|
PMID: 20164257
PDF is available here.
PDF is available here.
Abstract
The mechanism by which the embryo hatches out of the egg envelope, the vitelline membrane and egg white, was studied in the Chinese soft-shelled turtle Pelodiscus sinensis. The cDNA of the turtle hatching enzyme (HE) was 1555bp-long and a mature enzyme of 321 amino acids. The mature HE was composed...
|
PMID: 20109577
PDF is available here.
PDF is available here.
Abstract
The diameter of the lesion increased 24 hours after photocoagulation and then decreased by day 20. Most pyknotic nuclei seen in the outer nuclear layer 1 hour after lasering disappeared 3 days later. Remodeling began 3 days after lasering. By day 60, partial filling in of the empty area with sliding...
|
PMID: 19996833
PDF is available here.
PDF is available here.
Methods in Molecular Biology (638)638 2010
Abstract
We present methods to prepare fungi or plant tissues infected with fungi for transmission electron microscopy. This includes chemical fixations as well as cryofixation and the subsequent embedding in suitable resins. For cryofixation, the high pressure freeze fixation is described in detail. Further...
|
PMID: 20238278
PDF is available here.
PDF is available here.
Abstract
We report the three-dimensional (3D) structure of the membrane domain in an inhibitor-stabilized, outward-open conformation at 18A resolution. Unstained, frozen-hydrated tubular crystals containing the membrane domain of band 3 purified from human red blood cells (hB3MD) were examined using cryo-ele...
|
PMID: 20005958
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
We provide circumstantial evidence for a lower protein oxidative damage in CPD-SAGM-stored RBCs compared to the CPDA-stored cells. The different expression patterns of the senescence markers in the RBCs seem to be accordingly related to the oxidative stress management of the cells. We suggest that t...
|
PMID: 19874562
PDF is available here.
PDF is available here.
Abstract
We report the synthesis of colloidal palladium (cPd) nanoparticles of similar size but having two distinct shapes, umbonate and faceted, which are readily distinguishable from spherical colloidal gold particles. Their utility and fidelity as labels using a human platelet whole-mount model is also de...
|
PMID: 20030909
PDF is available here.
PDF is available here.
Abstract
During avian corneal development the nerves make close associations with a specialized type of ACE cell. There they form synapse-like structures, suggesting that not all nerves within the CE terminate as free nerve endings....
|
PMID: 19741242
PDF is available here.
PDF is available here.
médecine/sciences (26)2 2010
Abstract
Abstract
We have characterized its intracellular localization and function in the prostate cancer cell line LNCaP. Using light and electron microscopy, we identified myosin VI on Rab5-positive early endosomes, as well as on recycling endosomes and the trans-Golgi network. Intracellular targeting seems to inv...
|
PMID: 19855435
PDF is available here.
PDF is available here.
Journal of Neuroscience (30)3 2010
Abstract
We report that AP-3 and BLOC-1 differentially regulate the composition of presynaptic terminals in the striatum and dentate gyrus of the hippocampus. Quantitative immunoelectron microscopy demonstrated that the majority of AP-3 immunoreactivity in both wild-type striatum and hippocampus localizes to...
|
PMID: 20089890
PDF is available here.
PDF is available here.
Journal of Cell Biology (188)1 2010
Abstract
We provide evidence that the subunits of the conserved oligomeric Golgi (COG) complex are required for double-membrane cytoplasm to vacuole targeting vesicle and autophagosome formation. COG subunits localized to the phagophore assembly site and interacted with Atg (autophagy related) proteins. In a...
|
PMID: 20065092
PDF is available here.
PDF is available here.
Tsitologiia (52)9 2010
Abstract
We revealed MCs at different levels of their differentiation. Some MCs demonstrated an active process of granulogenesis in their cytoplasm. Among densely packed cytoplasmic organelles, immature secretory granules were closely associated with cisternae of RER and free ribosomes. Other MCs appeared as...
|
PMID: 21105364
PDF is available here.
PDF is available here.
Abstract
We show that F12 has structural similarity to kinesin light chain (KLC), a subunit of the kinesin-1 motor that binds cargo. F12 and KLC share similar size, pI, hydropathy and cargo-binding tetratricopeptide repeats (TPRs). Moreover, molecular modeling of F12 TPRs upon the crystal structure of KLC2 T...
|
PMID: 20195521
PDF is available here.
PDF is available here.
Abstract
We describe a protocol for transcardiac perfusion of mice with acrolein fixative, removal and sectioning of the brain, as well as immunoperoxidase-diaminobenzidine (DAB) staining, resin embedding, and ultrathin sectioning of the brain sections. Upon completion of these procedures, the immunostained...
|
PMID: 20689505
PDF is available here.
PDF is available here.
Abstract
The mitochondrial ATP-dependent K channel (mitoKATP) has been shown to play a role in cellular protection against apoptosis, or programmed cell death. This channel has been identified and characterized in a number of cell and tissue types but to date the possible existence of mitoKATP in osteoblasti...
|
PMID: 20036918
PDF is available here.
PDF is available here.
Abstract
We saw morphological differences between lymphocytes from MF and benign dermatoses. In MF, CD3 and CD4 were present rather in form of clusters placed on the surface of cell. On the contrary -CD3 to CD7 distribution analysis showed that these antigens were present rather individually, however there w...
|
PMID: 19895171
PDF is available here.
PDF is available here.
Methods in Enzymology (470) 2010
Abstract
We present a high-pressure freezing and freeze-substitution protocol which fulfills the key criterion for correlative microscopy, namely, the ability to achieve excellent visibility of fine structures without disrupting the antigens recognized by the immunolabeling protocol. This is achieved by a fi...
|
PMID: 20946827
PDF is available here.
PDF is available here.
Abstract
We induced cerebrovascular hypoperfusion by bilateral carotid occlusion surgery in adult rats and investigated its impacts on spatial memory, synapses, and accumulation of oligomeric amyloid-β. We found progressive spatial memory deficits, as tested by Morris water maze, starting 30 days after occl...
|
PMID: 20634588
PDF is available here.
PDF is available here.
BMC Gastroenterology (10)1 2010
Abstract
From the morphological appearance, it was impossible to distinguish terminal portal venules from small lymphatic vessels in the portal tract even using histochemical microscopic techniques. Recently, D2-40 was found to be expressed at a high level in lymphatic endothelial cells (LECs). This study wa...
|
PMID: 21059220
PDF is available here.
PDF is available here.
Abstract
We were able to detect the source of membranes following the breakup of cells using flow cytometry and immuno Electron Microscopy (immuno EM). Fractionation processes produced a small fraction of new membrane entities from two distinctively different origins generated during the initial disruption s...
|
PMID: 21060726
PDF is available here.
PDF is available here.
Nature Neuroscience (13)1 2010
Abstract
We show that continuous synthesis and availability of phosphatidylinositol-(3,4,5)-trisphosphate (PIP(3)) at the postsynaptic terminal is necessary for sustaining synaptic function in rat hippocampal neurons. This requirement was specific for synaptic, but not extrasynaptic, AMPA receptors, nor for...
|
PMID: 20010819
PDF is available here.
PDF is available here.
BMC Neuroscience (11) 2010
Abstract
Consistent with earlier findings, GT1b attenuated the DA neuron number and dopamine uptake level in mesencephalic cultures. Morphological evidence revealed GT1b-induced chromatin condensation and nuclear fragmentation as well as an increased number of TUNEL-positive cells, compared to control cultur...
|
PMID: 20540782
PDF is available here.
PDF is available here.
Abstract
Opsonins found on the surface of microbial cells in vivo and ex vivo are characterized. The possibility of their visualization by immune electron and fluorescent microscopy has been demonstrated. Opsonins are shown to play a role in immune-mediated adhesion of bacteria to phagocytes...
|
PMID: 21312383
PDF is available here.
PDF is available here.
Development (137)1 2010
Abstract
We use live cell imaging, super-resolution microscopy in fixed cells and immunoelectron microscopy on ultrathin frozen sections to study the distribution of Swallow, Staufen, actin and dynein relative to bicoid mRNA during late oogenesis. We show that Swallow and bicoid mRNA are transported independ...
|
PMID: 20023172
PDF is available here.
PDF is available here.
Abstract
We have identified cytoplasmic P-body components encoded by DHH1, KEM1, LSM1, and PAT1 as cofactors that posttranscriptionally enhance Ty1 retrotransposition. Using fluorescent in situ hybridization and immunofluorescence microscopy, we found that Ty1 mRNA and Gag colocalize to discrete cytoplasmic...
|
PMID: 19901074
PDF is available here.
PDF is available here.
Abstract
I Bcr-Abl specific pentamers). In addition, no proliferative response of T-cells of i.n. immunized mice after in vitro restimulation with antigen-pulsed bone marrow-derived dendritic cells was observed. Taken together, Bcr-Abl breakpoint epitopes appeared to be weak immunogens and even MPyV-VLPs did...
|
PMID: 19885546
PDF is available here.
PDF is available here.
Abstract
We assessed the importance of specific stretches of hydrophobic residues in driving the intrinsic ability of alpha-synuclein to polymerize. Several small deletions, even one with as few as two amino acid residues (A76 and V77), dramatically impaired the ability of alpha-synuclein to polymerize into...
|
PMID: 19722699
PDF is available here.
PDF is available here.
Abstract
We identified that whirlin is localized to chromosome 9q32-33, and is up-regulated in colorectal cancer tissues by using oligonucleotide array techniques and the Sosui system (http://www.tuat.ac.jp/~mitaku/sosui/). The deduced 920-amino acid protein encoded by the whirlin gene contains three PDZ dom...
|
PMID: 19724906
PDF is available here.
PDF is available here.
Abstract
We immunohistochemically examined the brain and spinal cord affected by two CAG repeat (polyglutamine) diseases, Machado-Joseph disease (MJD) and spinal and bulbar muscular atrophy (SBMA), using polyclonal antibody against TDP-43. In all the MJD cases, TDP-43-immunoreactive (ir) neuronal cytoplasmic...
|
PMID: 19526244
PDF is available here.
PDF is available here.
Avian Pathology (38)3 2009
Abstract
We describe a case of SCC on the underside of a pelican's foot. Histologically, the tumour consisted of irregular cords of pleomorphic epithelial cells that invaded the adjacent tissues. Additionally, keratinized epithelial cells and moderate numbers of keratin pearls were observed. Intracytoplasmic...
|
PMID: 19468940
PDF is available here.
PDF is available here.
Cell Host & Microbe (6)2 2009
Abstract
We observed that proteins associated with vacuolar membrane remnants are polyubiquinated, recruit the autophagy marker LC3 and adaptor p62, and are targeted to autophagic degradation. Further, inflammasome components and caspase-1 were localized to these membranes and correlated with dampened inflam...
|
PMID: 19683680
PDF is available here.
PDF is available here.
Abstract
We used immunofluorescence microscopy to characterise the presence and distribution of plateins, a sub-family of articulins, in T. gondii tachyzoites. A dispersed labelling of the whole protozoan body was observed. Electron microscopy of detergent-extracted cells revealed the presence of a network o...
|
PMID: 19820829
PDF is available here.
PDF is available here.
Abstract
Bestrophin-labeled gold particles were found in quasi-linear arrays on the basal surface of the macular RPE and also within drusen where bestrophin was found in segments of membranous-like material. The array density of the bestrophin-linked gold particles on the basal membrane of the epithelium had...
|
PMID: 19421767
PDF is available here.
PDF is available here.
Journal of Experimental Biology (212)Pt 15 2009
Abstract
We found that killifish CFTR (kfCFTR) and the tyrosine kinase focal adhesion kinase (FAK) phosphorylated at Y407 (FAK pY407) are colocalized in the apical membrane and in subjacent membrane vesicles of MR cells. We showed previously that basolateral FAK pY407, unlike other FAK phosphorylation sites,...
|
PMID: 19617429
PDF is available here.
PDF is available here.
Abstract
We have recently developed an immunoelectron microscopy (IEM) protocol adapted from the Tokuyasu method to prepare cryosections from mildly fixed yeast. This novel approach allows excellent cell preservation and a unique resolution of the yeast morphology. Here, we present a protocol that combines t...
|
PMID: 19435716
PDF is available here.
PDF is available here.