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Microscopy, Electron (5):
Recent article
Microscopy, Electron
Abstract
We show that C0 and C1 can each bind to the same two distinctly different positions on F-actin. One position aligns well with the previously reported binding site that clashes with the binding of myosin to actin, but would force tropomyosin into an "on" position that exposes myosin binding sites alo...
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PMID: 21821050
PDF is available here.
PDF is available here.
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Abstract
In this paper, a pH-inductive protein-scaffold biosynthesis of shape-tunable crystalline gold nanoparticles at room temperature has been developed. By simple manipulation of the reaction solution's pH, anisotropic gold nanoparticles including spheres, triangles and cubes could be produced by incubati...
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PMID: 21828895
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1812)9 2011
Abstract
We previously showed that in mice, lipopolysaccharide (LPS) induces a reversible and macrophage-dependent hepatic platelet accumulation (HPA), including translocation of platelets into Disse spaces and their entry into hepatocytes. Concanavalin A (ConA), which induces hepatitis in mice via both T ce...
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PMID: 21722729
PDF is available here.
PDF is available here.
Abstract
The biopolymer matrigel loaded with cytokine can be used for the recruitment in vivo of specific cell populations and as a vector for the preparation of cell cultures. Data demonstrate that the injection of the matrigel biopolymer supplemented with interleukin-8 (IL-8) in the leech H...
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PMID: 21400659
PDF is available here.
PDF is available here.
Ukrainskij biokhimicheskij zhurnal/Ukrayins'kij biokhimichnij zhurnal/Ukrainian biochemical journal (82)6 2011
Abstract
The influence of novel biologically active substance potassium 4-toluenethiosulfonate in concentration of 4 x 10(-5) M on the changes of electrophysiological parameters of embryonic cells in early development of fish (Misgurnus fossilis L.) was investigated including the changes of membrane potential...
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PMID: 21805861
PDF is available here.
PDF is available here.
Abstract
five of seven had a complete remission of the nephrotic syndrome and two of seven had a partial response. After a mean 24-month follow-up, only one patient experienced relapse of the nephropathy. Conclusions GN with monoclonal Ig deposits can be associated with MPGN or MN, which are correlated with...
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PMID: 21700823
PDF is available here.
PDF is available here.
Abstract
We studied the development of secretory cavities, including the role of parenchyma sheath, using light and transmission electron microscopy. A Tunel assay was performed to verify whether programmed cell death (PCD) occurs during the process. The lumen is formed by schizogeny and lysigeny occur in la...
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PMID: 21784363
PDF is available here.
PDF is available here.
Abstract
The ovary of the freshwater planarian Schmidtea mediterranea has been studied for the first time using both light and electron microscopy methods. The ultrastructure of the ovary revealed two types of cells: accessory cells and germinal cells at various stages of differentiation, dis...
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PMID: 21784361
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1812)7 2011
Abstract
We analyzed an osteoblast primary culture derived from the cervical vertebrae of a mandibuloacral dysplasia type A patient bearing the homozygous R527H LMNA mutation. Mandibuloacral dysplasia type A osteoblasts showed nuclear abnormalities typical of laminopathic cells, but they proliferated in cult...
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PMID: 21419220
PDF is available here.
PDF is available here.
Abstract
Our present findings, coupled with earlier work with the three-dimensional small intestinal INT407 model, demonstrate the utility of 3-D cell culture methods to develop infectivity assays for enteric viruses that do not readily infect mammalian cell cultures....
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PMID: 21942189
PDF is available here.
PDF is available here.
Abstract
We performed penetrating keratoplasty on the undiagnosed right eye. During post-surgical follow-up, corneal edema and stromal opacity recurred, and penetrating keratoplasty was performed two more times. The patient's total serum protein level, which had previously been normal, was elevated prior to...
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PMID: 21655047
PDF is available here.
PDF is available here.
Abstract
Abstract
The biotransformation of digitoxin and some of its derivatives extracted from Digitalis lanata by Streptomyces isolated species was investigated. Cultures of a Streptomyces strain designated EUSA2003B, isolated from an Egyptian soil sample, efficiently induced selective 12beta-hydroxylation of the st...
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PMID: 21699088
PDF is available here.
PDF is available here.
Klinichna khirurhiia / Ministerstvo okhorony zdorov'ia Ukrainy, Naukove tovarystvo khirurhiv Ukrainy ()6 2011
Abstract
Taking into account impossibility of reconstruction-restoration operations performance in cases of the distal arterial bed affection, it is necessary to find out and elaborate the methods of indirect revascularization. The cells transplantation performance for stimulation of reparation and angiogenes...
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PMID: 21846032
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
We have created an ophthalmic time-release prodrug form combined with a muco-adhesive lubricant compound carboxymethylcellulose and other essential corneal absorption promoter excipients tailoring the increased intraocular absorption of L-carnosine in the aqueous humor and optimizing its specific ef...
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PMID: 20408504
PDF is available here.
PDF is available here.
Abstract
These results revealed significant histological as well as ultrastructral lesions in the OTA treated group. The lesions included global congestion in the renal tissue and loss of demarcation between the cortex and medulla. The normal architecture of the renal corpuscles was destroyed and most of the...
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PMID: 21432769
PDF is available here.
PDF is available here.
Abstract
I included control rats injected only with liquid paraffin and saline; group II represents CCL(4) control (injected with CCL(4) 3 times a week for 6 weeks in a dose of 25μl/100gm.b.w i.p, diluted 1:6 with liquid paraffin); group III treated with aminoguanidine (100 mg/kg); group IV was given silyma...
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PMID: 21502683
PDF is available here.
PDF is available here.
Abstract
The purpose of this study was to form a standard electrical trauma model in rat, to investigate the pathological changes in vessels, and to determine the best day for performing microsurgery. In the preliminary study, 20 rats were divided into five groups. One was control, and the ra...
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PMID: 21467950
PDF is available here.
PDF is available here.
médecine/sciences (27)4 2011
Abstract
We raise here a few questions regarding the possible role of time as a key determinant of the metabolic energy budget, that impacts living cells from the organisation of macromolecular assemblies up to the morphogenesis and stabilisation of tissues....
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PMID: 21524409
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)10 2011
Abstract
We addressed how cationic amphipathic peptides-in particular a CAMP with Lysine-Leucine-Lysine repeats (termed KLK)-affect the localization and dynamics of molecules in eukaryotic membranes. We found KLK to selectively inhibit the endocytosis of a subgroup of membrane proteins and lipids by electros...
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PMID: 21718688
PDF is available here.
PDF is available here.
Development (138)8 2011
Abstract
We generated three mouse lines, each of which lacks one out of three prominent PCDH15 isoforms (CD1, CD2 and CD3). Surprisingly, mice lacking PCDH15-CD1 and PCDH15-CD3 form normal hair bundles and tip links and maintain hearing function. Tip links are also present in mice lacking PCDH15-CD2. However...
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PMID: 21427143
PDF is available here.
PDF is available here.
Abstract
Current Biology (21)5 2011
Abstract
We show that a conserved motif in the N-terminal region of Cenp-C binds directly and with high affinity to the Mis12 complex. Expression in HeLa cells of the isolated N-terminal motif of Cenp-C prevents outer kinetochore assembly, causing chromosome missegregation. The KMN network is also responsibl...
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PMID: 21353556
PDF is available here.
PDF is available here.
Presse Medicale (40)4 Pt 2 2011
Abstract
We consider that the intramuscle fiber degenerative component plays the primary and the major pathogenic role leading to muscle fiber destruction and clinical weakness. In this article we review the newest research advances that provide a better understanding of the s-IBM pathogenesis. Cellular abno...
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PMID: 21392932
PDF is available here.
PDF is available here.
Mutation Research (722)1 2011
Abstract
We have investigated the cyto- and genotoxic effects of single and multi-walled CNTs (SWCNTs, MWCNTs) and carbon black (CB) on the mouse macrophage cell line RAW 264.7. Specifically we have investigated inflammatory response, release of tumor necrosis factor-α (TNF-α), intracellular reactive oxyge...
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PMID: 21382506
PDF is available here.
PDF is available here.
Abstract
These results suggest that the rotavirus VP6, NSP1 and NSP5 genes of Wa-like rotaviruses are more prone to temporal mutations. Both structural and nonstructural genes of the Western Indian rotavirus strains shared nucleotide and amino acid substitutions with the Bangladeshi strain, Dhaka16-03 (G1P[8...
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PMID: 21256248
PDF is available here.
PDF is available here.
Saudi journal of kidney diseases and transplantation : an official publication of the Saudi Center for Organ Transplantation, Saudi Arabia (22)2 2011
Abstract
We describe a 42-year-old male patient who presented with high grade fever associated with acute renal failure requiring hemodialysis. Renal biopsy revealed that he had focal proliferative glomerulonephritis on light microscopy, dominant mesangial deposition of C1q by immunofluorescent staining, and...
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PMID: 21422635
PDF is available here.
PDF is available here.
Abstract
We have experienced two cases of intramyometrial cysts. One was a 6.5 cm-sized cyst and the other was a 3.0 cm-sized cyst accompanied by adenomyosis. Case 1 was a 41-year-old female and case 2 was a 51-year-old female who had been suffering from menorrhagia for several days. A total hysterectomy was...
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PMID: 21314810
PDF is available here.
PDF is available here.
Abstract
Erythropoietin (EPO) has protective effects on myocardial infarction (MI). This study was to test whether EPO administrated after MI is as effective as EPO administrated before MI and to determine the role of AMP-activated protein kinase (AMPK) in the cardioprotective effects of EPO. Recombinant huma...
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PMID: 21415704
PDF is available here.
PDF is available here.
Abstract
The protozoan Paramecium caudatum was examined under normal conditions versus aside a switched-on GSM telephone (900 MHz; 2 Watts). Exposed individuals moved more slowly and more sinuously than usual. Their physiology was affected: they became broader, their cytopharynx appeared bro...
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PMID: 21554102
PDF is available here.
PDF is available here.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] (27)2 2011
Abstract
Our present results indicated that RSIV was the causative agent....
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PMID: 21528541
PDF is available here.
PDF is available here.
Abstract
We describe the establishment of procedures to direct the differentiation of human embryonic stem cells and human induced pluripotent stem cells into forebrain neurons that are capable of forming synaptic connections. In addition, HEK293T cells expressing Neuroligin (NLGN) 3 and NLGN4, but not those...
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PMID: 21278334
PDF is available here.
PDF is available here.
Abstract
We analyze the role of Schmidtea mediterranea Dvl homologs (Smed-dvl-1 and Smed-dvl-2) using gene silencing. We demonstrate that in addition to a role in AP axis specification, planarian Dvls are involved in at least two different β-catenin-independent processes. First, they are essential for neura...
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PMID: 21282632
PDF is available here.
PDF is available here.
Abstract
We thus investigated the permissivity of three epithelial cell lines, primate Vero kidney cells, human A549 pneumocytes and human Ishikawa endometrial cells to this strict intracellular bacteria. Bacterial growth kinetics in these cell lines was assessed by quantitative PCR and immunofluorescence an...
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PMID: 21315828
PDF is available here.
PDF is available here.
Thrombosis and Haemostasis (105)4 2011
Abstract
A novel dysfibrinogenaemia with a replacement of Tyr by Asn at Bβ41 has been discovered (fibrinogen Caracas VIII). An asymptomatic 39-year-old male was diagnosed as having dysfibrinogenaemia due to a mildly prolonged thrombin time (+ 5.8 seconds); his fibrinogen concentration was in the low normal r...
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PMID: 21301788
PDF is available here.
PDF is available here.
Abstract
We clarified a unique molecular convergence of DGLα, CB(1), and MGL at specific inhibitory synapses in the basal nucleus (BA), but not lateral nucleus, of the basolateral amygdala. The synapses, termed invaginating synapses, consisted of conventional symmetrical contact and unique perisynaptic inva...
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PMID: 21282604
PDF is available here.
PDF is available here.
Abstract
By detecting the myelin structure in acoustic tumor tissues, the cell origin and state of acoustic tumor tissues were investigated.
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PMID: 21563465
PDF is available here.
PDF is available here.
Abstract
Our previous studies have shown that the hypoglossal nerve in hamsters contains somatic motor and postganglionic sympathetic fibers. The aim of this study was to investigate the ultrastructural changes in the nervous system following intraneural injection of mercuric chloride into the hypoglossal ne...
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PMID: 21377063
PDF is available here.
PDF is available here.
Abstract
We describe a 65-year old female with this diagnosis, who underwent an abdominal hysterectomy with bilateral salpingo-oophorectomy and lymph node dissection. Also included is a discussion on the different types of rhabdomyosarcoma with reviews of their histological features, epidemiology and common...
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PMID: 21476391
PDF is available here.
PDF is available here.
Abstract
Lysosomal storage diseases are a group of inherited disorders caused by deficiency of lysosomal enzymes or structural components. The manifestations of lysosomal storage diseases are complicated due to different enzyme deficiency. It has been reported that a range of metabolic diseases resulting in a...
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PMID: 21426694
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)4 2011
Abstract
We found that the PUN1 (YLR414c) gene is transcriptionally induced upon metal ion stress. Northern blot analysis of the transcriptional regulation of PUN1 showed that the calcium dependent transcription factor Crz1p is required for PUN1 induction upon heavy metal stress. Here we report that mutants...
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PMID: 21223946
PDF is available here.
PDF is available here.
Saudi journal of kidney diseases and transplantation : an official publication of the Saudi Center for Organ Transplantation, Saudi Arabia (22)4 2011
Abstract
We report an immune complex glomerulonephritis with "lupus-like" features in a ten-year-old HIV+ boy who was evaluated at the University Hospital of Kinshasa. The light microscopic examination of the renal biopsy displayed a predominantly membranoproliferative glomerulonephritis with prominent focal...
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PMID: 21743226
PDF is available here.
PDF is available here.
Saudi journal of kidney diseases and transplantation : an official publication of the Saudi Center for Organ Transplantation, Saudi Arabia (22)4 2011
Abstract
We report a patient who presented with features of acute glomerulonephritis. Kidney biopsy showed crescents in 30% of the glomeruli, diffuse mesangial expansion and hypercellularity with IgA deposits on immunofluorescence. The relevance of the Lee-Haas classification is discussed in relation to asse...
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PMID: 21743230
PDF is available here.
PDF is available here.
Abstract
The toxicity of naphthalene to a freshwater microalga, Chlorella pyrenoidosa, and the subsequent recovery of algae from the damage were investigated under two nutrient conditions, either enriched with nitrogen (N) and phosphorus (P), or starved of N and P. Results showed that C. pyre...
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PMID: 21517006
PDF is available here.
PDF is available here.
Abstract
Electron microscopy was used to study the peculiarities of the development of nervous elements in the sympathetic caudal mesenteric ganglion (CMG) in the cat from the moment of birth until the end of the second month of life. The discordance in the rate of maturation of both neurons and their endings...
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PMID: 21539084
PDF is available here.
PDF is available here.
Abstract
Tumor microenvironments present significant barriers to penetration by antibodies and immunoconjugates. Tumor microenvironments, however, are difficult to study in vitro. Cells cultured as monolayers exhibit less resistance to therapy than those grown in vivo and an alternative research model more re...
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PMID: 21305058
PDF is available here.
PDF is available here.
Abstract
We examined the structures of Escherichia coli Sec intermediates in solution as visualized by negatively stained electron microscopy in order to probe the oligomeric states of SecA during this process. The symmetric interaction pattern between the SecA dimer and SecB becomes asymmetric in the presen...
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PMID: 21304597
PDF is available here.
PDF is available here.
Abstract
We developed potential Drosophila models for Zellweger syndrome, in which the Drosophila pex3 or pex16 gene was disrupted. As found in Zellweger syndrome patients, peroxisomes were not observed in the homozygous Drosophila pex3 mutant, which was larval lethal. However, the pex16 homozygote lacking i...
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PMID: 21826223
PDF is available here.
PDF is available here.
Abstract
We established stable cell lines that ectopically over-express dusp12 in isolation and determined whether this cell line acquired properties frequently associated with transformed cells. Here, we demonstrate that cells over-expressing dusp12 display increased cell motility and resistance to apoptosi...
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PMID: 21556130
PDF is available here.
PDF is available here.
Abstract
ABSTRACT: BACKGROUND: primary tumours of the middle ear are much less commonly encountered in clinical practice than non neoplastic lesions. Middle ear adenocarcinoma is a very rare, locally invasive neoplasm assumed to arise from the middle ear mucosa. Because the natural course and...
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PMID: 21736736
PDF is available here.
PDF is available here.
Abstract
The cuticle of the longhorn beetle Pseudomyagrus waterhousei shows a diffuse pattern of mixed blue and violet colors. These colorations arise from a dense layer of droplet-shaped scales covering the dorsal parts of the cuticle. In spite of their lack of iridescence, these colors are...
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PMID: 21405714
PDF is available here.
PDF is available here.
Abstract
Nanodisperse powder of zinc-substituted magnetite has been developed. Functional characteristics (biocompatibility, dispersion, magnetic state) allow to recommend it for approbation in medical and biologic technologies. The character of the temperature dependences of magnetization investigated in the...
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PMID: 21796937
PDF is available here.
PDF is available here.
Abstract
We hypothesized that CXCL13 and CXCR5 could be involved in cardiac remodeling during HF.
We sought to analyze the role of the chemokine CXCL13 and its receptor CXCR5 in cardiac pathophysiology leading to HF.
Mice harboring a systemic knockout of the CXCR5 (CXCR5(-/-))...
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PMID: 21533157
PDF is available here.
PDF is available here.
Oncology Research (19)7 2011
Abstract
We determined the 50% embryo infective dose (EID50) and 50% tissue culture infective dose (TCID50) of the NDV strains. The MTT assay was used to evaluate the effects of NDV strains on cell viability. We determined the expression of Annexin V and Bcl-2 proteins in NDV-infected cells. Light microscopy...
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PMID: 21936402
PDF is available here.
PDF is available here.
Tsitologiia (53)3 2011
Abstract
We have shown partial loss of ribosomes and nucleolus inactivation in CA3 hippocampal pyramidal neurons in each bout of torpor with rapid and full recovery after warming up. Here we describe reversible structural changes in endoplasmic reticulum (ER) and Golgi complex (G) in these neurons. Transform...
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PMID: 21598689
PDF is available here.
PDF is available here.
Tsitologiia (53)5 2011
Abstract
Epithelium of the hepatic region of the intestine in Saccoglossus mereschkowskii, a representative of enteropneusts (Enteropneusta, Hemichordata) standing at the base of Chordata, has been investigated using electron microscope. The ultrastructure of ciliated and granular epithelial cells, elements o...
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PMID: 21786687
PDF is available here.
PDF is available here.
Abstract
The heterogeneity of tumor cell populations according to binding of lectins from lentil (LcL), wheat germs (WGA), peanut (PNA) and concanavalin A was investigated on a model of murine Nemeth-Kellner lymphoma (NK/Ly) and leukemia L-1210. Bound lectins were detected by indirect immunochemical method us...
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PMID: 21574425
PDF is available here.
PDF is available here.
Abstract
Abstract
Growth stimulation in potato Solanum tuberosum L. tubers by melafen preparation caused an increase in area ofmitochondrial apparatus (increase in mitochondrial size) in apical meristem cells. Melafen stimulated mitochondrial differentiation (increase in number of condensed mitochondria enriched in cr...
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PMID: 21950126
PDF is available here.
PDF is available here.
Abstract
Methods of obtaining cell walls (CW) for specimens of mucoraceous molds and ascomycetic affined fungi are developed at the stage of mycelium and resting cells, or spores. CW purity was assessed by electron microscopy, specific staining methods, scourage control, presence of ribose and desoxyribose, a...
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PMID: 21950120
PDF is available here.
PDF is available here.