Abstract
We used sequential high-abundance protein depletion and isobaric tag for relative and absolute quantitation liquid chromatography-tandem mass spectrometry to characterize the serum proteome in serum samples of patients with or without ACR. Seven of the 41 proteins identified as differentially abunda...
|
PMID: 21618694
PDF is available here.
Abstract
We defined the diagnostic criteria and prognostic value for proteins tested. In GC patients, the serum concentrations of all the proteins tested were significantly higher than in healthy subjects. The IL-6, CEA and CA 19-9 levels correlated with nodal metastases, while CRP with tumor stage, gastric...
|
PMID: 20938721
PDF is available here.
Abstract
To investigate whether allitridin could interfere with the effects of murine cytomegalovirus (MCMV) infection on the transcription, expression and function of IL-12 genes in order to further explore the mechanism of allitridin against MCMV.
|
PMID: 22007556
PDF is available here.
Abstract
Arterial stiffness is a risk marker for cardiovascular events. In this study we aimed to compare the effect on calcineurin inhibitors (CNI) and mammalian Target of Rapamycine inhibitors (mTORi) on arterial stiffness in renal transplant patients.
|
PMID: 21612751
PDF is available here.
Abstract
To determine the concentration of the pro-angiogenic vascular endothelial growth factor VEGF(165) (VEGF) and the anti-angiogenic VEGF(165b) in vitreous samples of patients with branch retinal vein occlusion (BRVO) and central retinal vein occlusion (CRVO) in comparison to patients without retinal occ...
|
PMID: 21621189
PDF is available here.
Abstract
To determine the levels of 23 immune mediators in paired aqueous humor (AqH) and serum samples from patients with birdshot chorioretinopathy (BSCR).
|
PMID: 21570674
PDF is available here.
Abstract
We have recently shown that antibody-induced blockade of C5a, C5a receptors, or IL-17A greatly reduced the harmful outcomes of sepsis. In the current study, normal cardiomyocytes from young (300 g) male Sprague-Dawley rats responded in vitro to C5a (ED(50)=55 nM) with release of IL-6 and TNFα, peaki...
|
PMID: 21478262
PDF is available here.
Abstract
We report a multicenter study to assess the potential of hybridoma and phage-display technologies in a coordinated large-scale antibody generation and validation effort. We produced over 1,000 antibodies targeting 20 SH2 domain proteins and evaluated them for potency and specificity by enzyme-linked...
|
PMID: 21572409
PDF is available here.
Abstract
IL-33 and its soluble receptor and cell-associated receptor (ST2L) are all increased in clinical and experimental asthma. The present study addressed the hypothesis that ST2L impairs the therapeutic effects of CpG in a fungal model of asthma. C57BL/6 mice were sensitized to Aspergillus fumigatus and...
|
PMID: 21640974
PDF is available here.
Abstract
Melittin liposomes surface modified with poloxamer 188 were developed, and the effect of poloxamer 188 was investigated with regard to anti-cancer effect and vascular stimulation. Melittin liposomes surface modified with poloxamer 188 at different concentrations (0%, 2%, and 5%) were prepared using t...
|
PMID: 21699070
PDF is available here.
Abstract
We speculate that he develops hypogammaglobulinemia secondary to the documented association between constrictive pericarditis and intestinal lymphangiectasia because an extensive work-up for a primary immunodeficiency was negative. This is the first case ever to present constrictive pericarditis bec...
|
PMID: 21634309
PDF is available here.
Abstract
We measured anti-p53 autoantibodies by ELISA in plasma samples from patients with normal cytogenetics as well as those with 13q, 11q, and 17p deletions as well as trisomy 12. Anti-p53 autoantibodies were detected in over half of the patients with a 17p deletion but in very few of the others. There w...
|
PMID: 21570119
PDF is available here.
Abstract
We describe a targeted approach for affinity improvement of therapeutic antibodies. Sets of CDR residues that are solvent accessible and relatively diverse in natural antibodies are targeted for diversification. Degenerate oligonucleotides are used to generate combinatorial phage-displayed antibody...
|
PMID: 19252851
PDF is available here.
Abstract
We describe a recombinant method for the construction and production of a novel IgG-like BsAb molecule, using the variable domains of two fully human antibodies as the building blocks....
|
PMID: 19252849
PDF is available here.
Jiri Mestecky,
Peter F Wright,
Lucia Lopalco,
Herman F Staats,
Pamela A Kozlowski,
Zina Moldoveanu,
Rashada C Alexander,
Rose Kulhavy,
Claudia Pastori,
Leonard Maboko,
Gabriele Riedner,
Yuwei Zhu,
Terri Wrinn and
Michael Hoelscher
Abstract
To address an existing controversy concerning the presence of HIV-1-specific antibodies of the IgA isotype in the female genital tract secretions of highly-exposed but persistently seronegative (HEPSN) women, 41 samples of plasma and cervicovaginal lavage (CVL) fluid were distributed...
|
PMID: 21091128
PDF is available here.
Susanne M Rittig,
Maik Haentschel,
Katrin J Weimer,
Annkristin Heine,
Martin R Muller,
Wolfram Brugger,
Marius S Horger,
Olga Maksimovic,
Arnulf Stenzl,
Ingmar Hoerr,
Hans-Georg Rammensee,
Tobias A W Holderried,
Lothar Kanz,
Steve Pascolo and
Peter Brossart
Abstract
The aim of this phase I/II nonrandomized trial was to assess feasibility, safety as well as immunological and clinical responses of a mRNA-based vaccination in patients with stage IV renal cell cancer using granulocyte-macrophage colony stimulating factor (GM-CSF) as adjuvant. Intrad...
|
PMID: 21189474
PDF is available here.
Abstract
We focused on mitochondria im pairment in the apoptotic process of MPP+ -elicited SH-SY5Y cells and detected the protection of puerarin. As evidenced by Trypan blue assay, the cell viability was significantly decreased by 1 mmol x L(-1) MPP+, but reversed by different concentrations puerarin pre tre...
|
PMID: 21842654
PDF is available here.
Abstract
To investigate the influences of extracts of Oxytropis falcata on proliferation of SMMC-7721 cells and expression of MMP-2 (matrix metalloproteinase-2).
|
PMID: 21842655
PDF is available here.
S S Tripathy,
A A Anand,
V V Inamdar,
M M MM Manoj,
K M KM Khillare,
A S AS Datye,
R R Iyer,
D M DM Kanoj,
M M Thakar,
V V Kale,
M M Pereira and
A R AR Risbud
Abstract
Background & objectives : In the Revised National Tuberculosis Control Programme (RNTCP) in India prior to 2005, TB patients were offered standard DOTS regimens without knowledge of HIV status. Consequently such patients did not receive anti-retroviral therapy (ART) and the influence of concomitant H...
|
PMID: 21623038
PDF is available here.
Abstract
Macrophage migration inhibitory factor (MIF) was originally described as a cytokine that inhibits migration of macrophages at the site of inflammation. Subsequently it was also identified as a stress-induced hormone released from the anterior pituitary lobe in response to some pro-in...
|
PMID: 21091281
PDF is available here.
Abstract
We evaluate the role of A(₂A)AR and its underlying signaling in retinal complications associated with diabetes. Initial studies in wild-type mice revealed that the treatment with the A(₂A)AR agonist resulted in marked decreases in hyperglycemia-induced retinal cell death and tumor necrosis facto...
|
PMID: 21514428
PDF is available here.
Abstract
Peroxisome proliferator-activated receptor-γ (PPARγ) is an anti-inflammatory molecule. To assess its biological function in lung alveolar epithelial cells, a CCSP-rtTA/(tetO)(7)-dnPPARγ bitransgenic mouse model was generated. In this model, a dominant negative (dn) PPARγ-Flag fusion protein was o...
|
PMID: 21514433
PDF is available here.