Find your PDFs fast.
Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Immunosorbent Techniques (4):
Recent article
Immunosorbent Techniques
Ukrainskij biokhimicheskij zhurnal/Ukrayins'kij biokhimichnij zhurnal/Ukrainian biochemical journal (82)6 2011
Abstract
The paper presents new information about the carbohydrate structures of 39-days chicken's fibronectin. It is found out that chicken fibronectin contains mainly biantennary N-glycans with a core fucose and fucosylated O-glycans. It is shown that N-glycans of chicken fibronectin are poorly sialated, si...
|
PMID: 21805863
PDF is available here.
PDF is available here.
| < More recent | Older article > |
MMW Fortschritte der Medizin (152)20 2010
Abstract
American Heart Journal (159)5 2010
Abstract
In patients with DCM, IA/IgG therapy may induce improvement in echocardiographic and cardiopulmonary exercise parameters.
2010 Mosby, Inc. All rights reserved....
|
PMID: 20435190
PDF is available here.
PDF is available here.
Proteomics (10)7 2010
Abstract
Efforts to discover protein biomarkers in plasma are hampered by the high abundance of few proteins, which interfere with the detection of low-abundant proteins. Different commercially available protein-partitioning products were tested for their ability to lower the detection limit...
|
PMID: 20127685
PDF is available here.
PDF is available here.
Abstract
We present our experience with extracorporeal immunoglobulin (Ig) elimination by immunoadsorption (adsorbers with human Ig antibodies). Acetylcholine receptor antibodies (AChRAs) were measured during long-term monitoring (4.7 +/- 2.9 years; range 1.1-8.0). A total of 474 samples (232 pairs) were ana...
|
PMID: 20300435
PDF is available here.
PDF is available here.
BMC Nephrology (11) 2010
Abstract
Plasma exchange is effective in patients with severe ARF secondary to vasculitis. Pulse cyclophosphamide results in an increased risk of relapse when compared to continuous oral use but a reduced total dose. Whilst cyclophosphamide is standard induction treatment, rituximab and mycophenolate mofetil...
|
PMID: 20573267
PDF is available here.
PDF is available here.
Dermatology (220)3 2010
Abstract
We report a patient with a severe generalized LAD in whom adjuvant tryptophan IA was associated with rapid healing of skin lesions. Our observation suggests that IA may also be a helpful adjuvant treatment option for severe LAD.
2010 S. Karger AG, Basel....
|
PMID: 20130384
PDF is available here.
PDF is available here.
Abstract
I and human platelet antigens (HPA). Sera from 38 thrombocytopenic patients and 5 mothers of thrombocytopenic newborns were screened for platelet reactive antibodies by these three methods using screening platelets and/or lymphocytes panels derived from six subjects. The sensitivity and specificity...
|
PMID: 21038788
PDF is available here.
PDF is available here.
Abstract
Immunoadsorption (IAS) with various methods is used as a rescue therapy in severely ill SLE patients who are refractory to conventional therapeutic procedures. The method aims at the rapid and extensive removal of pathogenic immunocomplexes (IC) and (auto-) antibodies (Abs). Long-ter...
|
PMID: 20129387
PDF is available here.
PDF is available here.
Abstract
The novel application of the immunochromatography assay to assess the total IgE in tears is a useful clinical tool to investigate ACD....
|
PMID: 19779297
PDF is available here.
PDF is available here.
Abstract
A 49-year old patient with chronic kidney disease was referred for evaluation of living donor kidney transplantation from his spouse in a blood group incompatible setting. CLINICAL INVESTIGATIONS: Patient blood group was 0, donor blood group was A, subtype A. recipient isoagglutinin titer against do...
|
PMID: 20013598
PDF is available here.
PDF is available here.
Abstract
Thrombocytopenia and hemolytic anemia, neurologic symptoms, and renal dysfunction were the clinical manifestations in 8 patients. Six patients had fever. Eight patients presented with rapid progressive glomerulonephritis, and 1 patient with continuous gross hematuria. The histologic features of the...
|
PMID: 19745703
PDF is available here.
PDF is available here.
Annals of Hematology (88)4 2009
Abstract
We performed a survey among 73 physicians from 57 haemophilia treatment centres in order to describe current practice patterns and critical issues for future research in acquired haemophilia. The results demonstrate a high diversity of first- and second-line treatments. Factors influencing treatment...
|
PMID: 19083235
PDF is available here.
PDF is available here.
Parasitology (92 ( Pt 2))02 2009
Abstract
Polyclonal antisera raised against Plasmodium knowlesi reacted with NADP-specific glutamate dehydrogenase (GLDH) of P. knowlesi, GLDH of P. falciparum and GLDH of Proteus spp. The antisera did not react with NAD(P) GLDH from bovine liver. Polyclonal antisera raised against the GLDH of Proteus spp. c...
|
PMID: 3086819
PDF is available here.
PDF is available here.
Abstract
Thirty-three episodes of MG crisis with respiratory failure from 26 patients (9 males and 17 females) were documented. Plasmapheresis and IVIG were prescribed in 21 and 9 episodes of MG crisis, respectively. There was no statistical significant difference in baseline characteristics between both gro...
|
PMID: 19374297
PDF is available here.
PDF is available here.
Thrombosis and Haemostasis (101)3 2009
Abstract
Abstract
We developed five lectin immunosorbant assays to analyze the glycosylation patterns of PSA in serum. Each assay sandwiches serum PSA between a PSA monoclonal antibody and a biotinylated lectin and then tags the biotin complex using a streptavidin SULFO TAG for electrochemiluminescence detection. Low...
|
PMID: 19035787
PDF is available here.
PDF is available here.
Immunogenetics (61)2 2009
Abstract
We investigated the polymorphisms that are recognized by antibodies against MICA. Soluble MICA recombinant proteins representing 11 common alleles, two hybrid alleles, and two single amino acid mutated alleles were produced. Patterns of reactivity were determined with MICA bound to Luminex beads. In...
|
PMID: 19066881
PDF is available here.
PDF is available here.
Vojnosanitetski pregled (66)2 2009
Abstract
The pre-transplant treatment consisting of 1-5 PE procedures and immunosuppressive therapy resulted in target ABO agglutinins titers below 4. During a 10-24 month follow-up three patients had an early acute rejection, one patient acute rejection and hemolytic anemia, two patients surgical complicati...
|
PMID: 19281122
PDF is available here.
PDF is available here.
Abstract
The results indicate that the methods compared are all adequate for diagnosing Legionella infection, although some have certain limitations regarding sensitivity....
|
PMID: 19254639
PDF is available here.
PDF is available here.
Abstract
We describe a new method based on polypyrrole chemistry, allowing the covalent immobilization of peptides in a microarray format and on a gold surface compatible with the use of surface plasmon resonance. This technique is then illustrated by the detection and characterization of antibodies induced...
|
PMID: 19649603
PDF is available here.
PDF is available here.
Journal of Internal Medicine (234)5 2009
Abstract
Objective. Immunoadsorption (IMA) and dextran sulfate adsorption (DSA) are two methods for selective extracorporeal elimination of low-density lipoproteins which are known as LDL apheresis. Their influence on haemostasis until now is widely unknown. Design. The effects of both LDL apheresis procedure...
|
PMID: 8228792
PDF is available here.
PDF is available here.
Abstract
We compared the concentrations of remnant lipoproteins as RemL-C with those as RLP-C in various conditions. RemL-C assay was intra-assay-reproducible (n=20, CVs: 0.6-2.2%), and reproducible for 2 days in the refrigeration and for 8 hours in room temperature. This assay was also inter-assay-reproduci...
|
PMID: 19227187
PDF is available here.
PDF is available here.
Abstract
The immunosuppressive drug therapy (IDT) is not always effective to avoid the development of complications in hepatitis C virus-related cryoglobulinemia (HCV-Cr). Removal of cryoglobulins by therapeutic plasmapheresis is currently accepted. In this randomized, parallel group study, 1...
|
PMID: 19927363
PDF is available here.
PDF is available here.
Transplantation (86)8 2008
Abstract
Normal males were found to have HLA antibodies to infrequent HLA specificities. It is likely that these HLA antibodies are produced to cross-reactive epitopes found in microorganisms, ingested proteins and allergens-making them natural antibodies....
|
PMID: 18946350
PDF is available here.
PDF is available here.
Abstract
Average duration of analyzed apheresis treatment was 6.4 years, with a mean treatment-interval of 1.1 per week. Mean reduction rate of autoantibodies against acetylcholine-receptor-protein was 50-60 % per session. After initiation of immunoadsorption the mean time of hospitalisation decreased signif...
|
PMID: 18988129
PDF is available here.
PDF is available here.
Abstract
We have learned that these relatively new protocols carry very promising short-term and intermediate-term results which compare favorably to the outcome of ABO-compatible living donor transplantations. Latest reports suggest that combining immunoadsorptions with rituximab does not result in an incre...
|
PMID: 19001813
PDF is available here.
PDF is available here.
Transplantation (86)3 2008
Abstract
Three DQA1 and 15 DQB1 epitopes were identified. We found that 21 patients produced antibodies against one of the DQA1 epitopes; 27 patients produced antibodies against one of the DQB1 epitopes. CONCLUSION: The DQA1 and DQB1 epitopes identified here seem to be immunogenic and to elicit DQ antibodies...
|
PMID: 18698250
PDF is available here.
PDF is available here.
Transplantation (86)3 2008
Abstract
We describe the evolution in laboratory technology, the techniques currently available and consider their application in antibody specificity definition and in understanding a patient's sensitization profile. We discuss the importance of antibody specificity definition in facilitating efficient nati...
|
PMID: 18698240
PDF is available here.
PDF is available here.
Abstract
Abstract
We explore all parameters for effective use of fluorescence as a plate based detection method, including which microtiter plates can be used, the most effective means of immobilization, and the use of different fluorescent dyes or fluorescent proteins. These studies indicate that fluorescent immunos...
|
PMID: 18514691
PDF is available here.
PDF is available here.
Abstract
We need to (a) evaluate the potential role of autoantibodies in disease prevention and progression, and understand their importance as markers of disease progression; (b) clarify the role of immunoregulation in exacerbating the disease....
|
PMID: 18545064
PDF is available here.
PDF is available here.
Analytica Chimica Acta (618)1 2008
Abstract
The use of accurate mass measurement as a confirmation tool is examined on a TOF-MS and compared with confirmation using a triple quadrupole mass spectrometer (QqQ-MS). Confirmation of the identity of a substance using mass-spectrometric detection has been described. However, the use of accurate mas...
|
PMID: 18501249
PDF is available here.
PDF is available here.
Tissue Antigens (71)6 2008
Abstract
I and II antibodies in recipient sera. Many laboratories rely on their specificity detection and use the information obtained for allocation of donor organs. A donor-specific crossmatch in the Luminex technique (LumXm) is for that reason desirable. A LumXm, in which the actual donor HLA are coated o...
|
PMID: 18380779
PDF is available here.
PDF is available here.
Abstract
This review describes the three ways of plasmapheresis in patients with myasthenia gravis: plasma exchange (PE), double filtration plasmapheresis (DFP) and immunoadsorption (IA). DFP or IA was used for selective removal of antibodies to acetylcholine receptor without replacement of albumin. Plasmaph...
|
PMID: 18540364
PDF is available here.
PDF is available here.
Journal of Immunological Methods (335)1-2 2008
Abstract
We compared carboxylated and Penta-His beads. For carboxylated as compared to Penta-His beads, the non-specific background is lower (Median Fluorescence Intensity; MFI>250, 0% versus 15%), the specific signal intensity is higher (mean MFI 2860 versus 722) and not dependent on the configuration of th...
|
PMID: 18417151
PDF is available here.
PDF is available here.
Environmental Research (107)2 2008
Abstract
An AOZ method, based on high-performance liquid chromatography (HPLC), was optimized on HPLC condition such as mobile phase and wavelength to simultaneously quantify six kinds of mycotoxins [four aflatoxins (AFs), ochratoxin A (OTA) and zearalenone (ZEA)]. Conditions for immunoaffinity clean-up, HPL...
|
PMID: 18313042
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (133)6 2008
Abstract
The versatility of immunoassays for the detection of antigens can be combined with the signal amplification power of nucleic acid amplification techniques in a broad range of innovative detection strategies. This review summarizes the spectrum of both, DNA-modification techniques used for assay enha...
|
PMID: 18493669
PDF is available here.
PDF is available here.
Abstract
Journal of Hypertension (26)5 2008
Abstract
Angiotensin (Ang) II in cardiac, renal and adrenal tissue is known to originate mainly from locally produced Ang I. Experimental evidence and theoretical considerations show that a simple relation between Ang II receptor occupancy, in tissue micromilieu, and the circulating levels of Ang II or renin...
|
PMID: 18398335
PDF is available here.
PDF is available here.
Abstract
We have developed a simple and sensitive method for an immunoassay based on surface-enhanced resonance Raman scattering (SERRS). For the first time, a SERRS-based immunoassay on the bottom of a microtiter plate is reported. We have applied the main pretreatment method of enzyme-linked immunoabsorben...
|
PMID: 18318511
PDF is available here.
PDF is available here.
Abstract
In a multicenter study a new, fully automated Roche Diagnostics Elecsys HBsAg II screening assay with improved sensitivity to HBsAg mutant detection was compared to well-established HBsAg tests: AxSYM HBsAg V2 (Abbott), Architect HBsAg (Abbott), Advia Centaur HBsAg (Bayer) Enzygnost HBsAg 5.0 (Dade-...
|
PMID: 17899185
PDF is available here.
PDF is available here.
Journal of Neuroimmunology (194)1-2 2008
Abstract
These results demonstrate that a minor disease-specific immunoglobulin fraction present in IVIG is essential for its suppressive activity....
|
PMID: 18178258
PDF is available here.
PDF is available here.
Abstract
We have examined interactions between AVA (mAb and serum from patients) and various leukocyte populations using whole blood and flow cytometry. Normal blood treated with patient sera containing high AVA-IgM titers or with a vimentin-specific monoclonal IgM led to activation of platelets and other le...
|
PMID: 17974709
PDF is available here.
PDF is available here.
Abstract
All seven patients showed a sharp decline of circulating autoantibody levels and rapid improvement of cutaneous and mucosal lesions within 4 weeks of therapy. Long-term remission was induced in three patients and one further patient showed a partial improvement of his disease. The three remaining pa...
|
PMID: 18070210
PDF is available here.
PDF is available here.
BMC Biochemistry (9)1 2008
Abstract
Purifying BDH from jerboa liver overcomes its low specific activity in mitochondria for further biochemical characterization of the enzyme. This new procedure is based on the use of polyclonal antibodies raised against BDH from bacterial Pseudomonas aeruginosa. This study improves the procedure for...
|
PMID: 18826626
PDF is available here.
PDF is available here.
Abstract
ABO-incompatible kidney transplantation seems to be successful and safe. Modifications of the current protocol may be possible and may further reduce potential side effects and costs....
|
PMID: 18728155
PDF is available here.
PDF is available here.
Nutrition and Cancer (60)4 2008
Abstract
We show that a fraction [nondialyzable material (NDM) of a molecular weight range 12,000-30,000 (NDM 12-30K)] derived from cranberry juice impairs in vitro growth and invasion through extracellular matrix of Rev-2-T-6 murine lymphoma cells. Furthermore, intraperitoneal injection of this fraction at...
|
PMID: 18584485
PDF is available here.
PDF is available here.
Journal of Endocrinology (196)1 2008
Abstract
These results suggest a potential novel mechanism for progesterone's immunoregulatory function through activation of mPRs....
|
PMID: 18180318
PDF is available here.
PDF is available here.
Abstract
Cardiac autoantibodies play a pathogenic role in dilated cardiomyopathy (DCM). Removal of antibodies by immunoadsorption (IA) induces hemodynamic improvement in DCM patients. The present study investigated the effects of IA on myocardial gene expression of the intermediate cytoskeletal filament desm...
|
PMID: 17924085
PDF is available here.
PDF is available here.
Transplantation Proceedings (39)10 2007
Abstract
We report on PE and IA in the treatment of two children with recurrent nephrotic proteinuria. Patient 1 was a 16-year-old girl who had recurrence of nephrotic proteinuria on the first day after transplantation (proteinuria-19 g/d). Primary immunosuppressive therapy was changed to high-dose cyclospor...
|
PMID: 18089416
PDF is available here.
PDF is available here.
Journal of Immunological Methods (328)1-2 2007
Abstract
We propose a test system based on a proteomics screen to simultaneously identify proteins adsorbed from serum to the surface of silicone. Herein, we describe protein adsorption kinetics on the surface of silicone implants, correlate the adhesion properties of serum proteins with the occurrence of ad...
|
PMID: 17920619
PDF is available here.
PDF is available here.
Nature Methods (4)11 2007
Abstract
We describe a rapid affinity-purification method for efficient isolation of the subcomplexes that dynamically organize different RNP biogenesis pathways in Saccharomyces cerevisiae. Our method overcomes many of the previous limitations to produce large RNP interactomes with almost no contamination....
|
PMID: 17922018
PDF is available here.
PDF is available here.
Abstract
Abstract
Dilated cardiomyopathy (DCM) is a common myocardial disease characterized by ventricular dilatation and progressive depression of myocardial contractile function. Disturbances in both humoral and cellular immunity have been described among these patients. A number of antibodies against various cardi...
|
PMID: 18036989
PDF is available here.
PDF is available here.
Abstract
A shorter pre-operative length on dialysis, a blood-type constellation of donor A1/recipient 0 and 9a high initial starting-titer were identified as predictors for post-operative immunoadsorptions. CONCLUSION: Using this on-demand strategy, our data reveal that a titer-dependent protocol reduces cos...
|
PMID: 17623716
PDF is available here.
PDF is available here.
Transfusion (47)10 2007
Abstract
Adverse reactions, most minor, were reported in 55 percent of procedures in 82 percent of patients. The most frequent complications, per procedure and per patient during an entire course of therapy, were hypotension (14 and 48.4%), hypotension requiring fluid bolus (4.8 and 26.9%), symptomatic hypoc...
|
PMID: 17880609
PDF is available here.
PDF is available here.
Journal of Neuroimmunology (190)1-2 2007
Abstract
We used the N-terminal extracellular domain (ECD) of the AChR alpha subunit to immunoadsorb anti-alpha subunit autoantibodies from MG sera. In this study, we immobilised the beta -, gamma- and epsilon-AChR ECDs on Sepharose and tested them as immunoadsorbents on 50 MG sera. A given ECD removed a dif...
|
PMID: 17764755
PDF is available here.
PDF is available here.
Journal of Neuroimmunology (189)1-2 2007
Abstract
We have studied nAChR antigenic modulation caused by isolated anti-subunit autoantibodies. Autoantibodies against the nAChR alpha or beta subunits were isolated from four MG sera by affinity chromatography on columns carrying immobilized recombinant extracellular domains of human nAChR expressed in...
|
PMID: 17617475
PDF is available here.
PDF is available here.
Abstract
We observed inhibition of anti-CD45, but not anti-CD3, -CD4, or -CD8 staining in the whole blood of an individual clinical specimen. Subsequent experiments demonstrated the presence of a plasma factor, which likewise blocked CD45 staining of third party lymphocytes. The blocking activity was anti-CD...
|
PMID: 17311353
PDF is available here.
PDF is available here.