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Immunoassay (4):
Recent article
Immunoassay
Abstract
We tested the hypothesis that circulating PICP and PIIINP are altered and may correlate with ventricular volume load and function in patients with repaired tetralogy of Fallot (TOF).
Serum PICP and plasma PIIINP levels were determined in 39 patients with repaired TOF aged 17.7 ± 4.1...
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PMID: 21884862
PDF is available here.
PDF is available here.
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Abstract
Federal Register (76)153 2011
Abstract
The Food and Drug Administration (FDA) is amending the special controls for the herpes simplex virus (HSV) serological assay device type, which is classified as class II (special controls). These device types are devices that consist of antigens and antisera used in various serologic...
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PMID: 21826824
PDF is available here.
PDF is available here.
Abstract
A rapid and simple method for the simultaneous quantification of AFB1 and OTA in rat plasma, liver and kidney by UHPLC-FLD has been successfully validated according to the following criteria: selectivity, stability, linearity, precision, accuracy, recovery, robustness and limits of q...
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PMID: 21868292
PDF is available here.
PDF is available here.
Abstract
We obtained and analysed samples of blood serum from 23 patients with MF, and 29 psoriasis patients and 22 healthy donors as controls. Protein profiling was carried out using SELDI TOF MS SELDI-TOF and also profiling of 27 cytokines with multiplex immunoassay technology was implemented....
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PMID: 21751413
PDF is available here.
PDF is available here.
Abstract
We aimed to analyze the diagnostic and prognostic potential of a newly developed high-sensitive troponin T assay and compared these results with those of a contemporary troponin T assay in 2 distinct patient cohorts, one including patients with evident ACS and the other one including patients with g...
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PMID: 21742093
PDF is available here.
PDF is available here.
Abstract
The data is presented concerning the implementation of nano-particles of aurum in the diagnostics of systemic lupus erythematosus. It is demonstrated that nano-particles sensibilized by deoxyribonucleoprotein are able to detect the specific antibodies--the known markers of systemic l...
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PMID: 21901893
PDF is available here.
PDF is available here.
Abstract
We present an overview of the challenges and approaches to overcome them when performing quantitative bioanalysis of peptides and proteins by LC-MS/MS....
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PMID: 21679032
PDF is available here.
PDF is available here.
Ugeskrift for laeger (173)19 2011
Abstract
Abstract
We used DIGE to compare protein expression in amastigote and promastigote forms of Leishmania chagasi. Nine hundred amastigote and promastigote spots were visualized. Five amastigote-specific, 25 promastigote-specific, and 10 proteins shared by the two parasite stages were identified. Furthermore, 4...
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PMID: 21355625
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
Many biosensors depend on molecular recognition reagents to achieve highly sensitive and specific detection levels of an analyte of interest. Although new and improved detection platforms continue to be developed, improvements in the affinity and specificity of the molecular recognition reagents oft...
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PMID: 19159106
PDF is available here.
PDF is available here.
Abstract
This review focuses on the toxicity and metabolism of T-2 toxin and analytical methods used for the determination of T-2 toxin. Among the naturally occurring trichothecenes in food and feed, T-2 toxin is a cytotoxic fungal secondary metabolite produced by various species of Fusarium. Following ingest...
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PMID: 21417259
PDF is available here.
PDF is available here.
Abstract
I monoclonal antibodies (Mabs) were prepared by hybridoma technique and firstly used to develop a Sudan I immunosensor by immobilizing the Mabs on a gold electrode. o-Mercaptobenzoic acid (MBA) was covalently conjugated on the gold electrode to form a self-assembled monolayer (SAM). The immobilizati...
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PMID: 21315921
PDF is available here.
PDF is available here.
Abstract
A 35-year-old female presented with complaints of tiredness, shortness of breath and recent onset of chest pain. The cardiac troponin I (cTnI) concentration was elevated at 6.4 μg/L (Abbott Architect reference value:
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PMID: 21388341
PDF is available here.
PDF is available here.
Abstract
To determine the characteristics of obese children presenting at a tertiary care hospital and the frequency of metabolic syndrome (MS) in them using two paediatric definitions.
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PMID: 21419020
PDF is available here.
PDF is available here.
Abstract
These results indicate that the PATH-Lemos rapid test shows promise as an improved and reliable tool for screening and diagnosis of Chagas disease....
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PMID: 21342808
PDF is available here.
PDF is available here.
Abstract
We assessed whether immune activation would differentially influence such outcomes as a function of stressor reactivity related to genetic differences. To this end, we assessed the influence of a social stressor (exposure to a dominant mouse) in combination with an acute immune challenge on behavior...
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PMID: 21093579
PDF is available here.
PDF is available here.
Lab On a Chip (11)1 2011
Abstract
We report a fully integrated device that can perform both multiple biochemical analysis and sandwich type immunoassay simultaneously on a disc. The whole blood is applied directly to the disposable "lab-on-a-disc" containing different kinds of freeze-dried reagents for the blood chemistry analysis a...
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PMID: 21042620
PDF is available here.
PDF is available here.
Abstract
We have examined the effect of grape seed proanthocyanidins (GSPs) on melanoma cancer cell migration and the molecular mechanisms underlying these effects using highly metastasis-specific human melanoma cell lines, A375 and Hs294t. Using in vitro cell invasion assays, we observed that treatment of A...
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PMID: 21738696
PDF is available here.
PDF is available here.
Abstract
We have investigated the presence of Th17 cells in the mucosa of active CD patients and their functional implications in the pathogenesis of the disease....
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PMID: 21206487
PDF is available here.
PDF is available here.
Abstract
We investigated expressional changes in the hippocampus and cortex at 72 h after infection when dexamethasone was given to infant rats with pneumococcal meningitis. Nursing Wistar rats were intracisternally infected with Streptococcus pneumoniae to induce experimental meningitis or were sham-infecte...
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PMID: 21412436
PDF is available here.
PDF is available here.
Acta Biomaterialia (7)1 2011
Abstract
We therefore aimed to investigate whether bone-implant interface strength and osseointegration of a novel biodegradable magnesium alloy (Mg-Y-Nd-HRE, based on WE43) is comparable to that of a titanium control (Ti-6Al-7Nb) currently in clinical use. Biomechanical push-out testing, microfocus computed...
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PMID: 20804867
PDF is available here.
PDF is available here.
Abstract
We present the method for developing a custom CHO-HCP AMMP assay for bioprocess development. Developing AMMP assays or modifying existing assays into AMMP assays can provide better performance (reproducibility, sensitivity) in complex samples and reduced operator time. The protocol shows the steps f...
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PMID: 21339717
PDF is available here.
PDF is available here.
Abstract
We describe a strategy, termed in vivo microbial antigen discovery (InMAD), that we used to identify circulating bacterial antigens. This technique starts with "InMAD serum," which is filtered serum that has been harvested from BALB/c mice infected with a bacterial pathogen. The InMAD serum, which i...
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PMID: 21846829
PDF is available here.
PDF is available here.
Abstract
We describe how to extract and quantify Rubisco activase content in leaf....
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PMID: 20960145
PDF is available here.
PDF is available here.
Abstract
Botulinum type A toxin (BoNT/A) is defined by its specific endopeptidase cleavage of SNAP25 between Gln(197) and Arg(198) under reducing conditions. The neurotoxin is widely used for therapeutic or cosmetic purposes, but should not contain other toxin serotypes or unwanted protease activities. Using...
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PMID: 20832960
PDF is available here.
PDF is available here.
Lab On a Chip (10)23 2010
Abstract
We demonstrate for the first time continuous DNA extraction from cell lysate on a microfluidic chip. After mixing bacterial Escherichia coli culture with superparamagnetic bead suspension, lysis and binding buffers, DNA is released from cells and captured by the beads. These DNA carrying beads are c...
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PMID: 20938545
PDF is available here.
PDF is available here.
Abstract
We report development of a low-cost dipstick p24 antigen assay using a visual readout format that can facilitate the diagnosis of HIV for infants in resource-poor conditions. A heat shock methodology was developed to optimize disruption of immune complexes present in the plasma of infected infants....
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PMID: 20811289
PDF is available here.
PDF is available here.
Mutation Research (711)1-2 2011
Abstract
We highlight the potential of a new technology allowing direct visualization of different types of DNA lesions in human cells and document the emerging significance of live-cell imaging for elucidation of the spatio-temporal characterization of complex DNA damage. We focus on the recent insights int...
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PMID: 21126526
PDF is available here.
PDF is available here.
Abstract
We developed a Clinical Data Mining Software to collect cytokine profiles evaluated on healthy subjects and patients by multiplex immunoassays also annotated with their clinical and laboratory data, to compare patient profiles by statistical tools and to evaluate their disease progression....
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PMID: 20889532
PDF is available here.
PDF is available here.
Journal of Glaucoma (19)9 2010
Abstract
To investigate whether there is an association of elevated plasma levels of homocysteine (Hcy) and normal tension glaucoma (NTG).
Plasma levels of Hcy (fluorescence polarization immunoassay), vitamin B6 (high-performance liquid chromatography), vitamin B12, and folate levels (immunoassay) were deter...
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PMID: 20179629
PDF is available here.
PDF is available here.
Lab On a Chip (10)22 2010
Abstract
We have demonstrated that 0.4 and 1 μm beads conjugated with antibodies can be rapidly assembled into the microwells by applying a pulsed electric field across the chamber. By assembling step-wise a mixture of fluorescently labeled antibody-conjugated microbeads, we incorporated both spatial and fl...
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PMID: 20820631
PDF is available here.
PDF is available here.
Lab On a Chip (10)22 2010
Abstract
We describe an automated, self-powered chip based on lateral flow immunoassay for rapid, quantitative, and multiplex protein detection from pinpricks of whole blood. The device incorporates on-chip purification of blood plasma by employing inertial forces to focus blood cells away from the assay sur...
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PMID: 20924527
PDF is available here.
PDF is available here.
Chemical Communications (46)42 2010
Abstract
A promising one-step homogeneous fluoroimmunoassay based on nanoscale-graphene sheets as powerful fluorescence acceptors and CdTe quantum dots as vigorous donors was designed to detect trace biomarker protein with distance-independent quenching efficiency, which significantly broke the distance limi...
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PMID: 20856985
PDF is available here.
PDF is available here.
Immunotherapy (2)6 2010
Abstract
We highlight potential sources of variability, both due to assay nuances and the diversity of human populations. We present strategies for analyzing data, emphasizing both multidimensional analysis and the value of treating each donor as his or her own control....
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PMID: 21091113
PDF is available here.
PDF is available here.
Thrombosis and Haemostasis (104)5 2010
Abstract
We hypothesised that plasma exchange (PE) is efficient for lowering excess Ang-2 levels in critically ill patients with thrombocytic microangiopathy (TMA) or anti-glomerular basement membrane (anti-GBM) disease. Plasma Ang-1 and Ang-2 were measured by immuno-luminometric assays in patients with TMA...
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PMID: 20806109
PDF is available here.
PDF is available here.
Thrombosis and Haemostasis (104)5 2010
Abstract
We aimed to investigate the complication rate of untreated isolated calf DVT (ICDVT). Symptomatic outpatients were prospectively managed with serial compression ultrasonography (SCUS). Those without proximal DVT and with likely pre-test clinical probability (PCP) or altered D-dimer received immediat...
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PMID: 20694281
PDF is available here.
PDF is available here.
Abstract
This article reviews the relevance of the following areas to the contemporary management of cast nephropathy in multiple myeloma: immunoassays that quantify immunoglobulin free light chain (FLC), novel chemotherapy agents and high cut-off (protein-permeable) haemodialysis, which are under evaluation...
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PMID: 20827195
PDF is available here.
PDF is available here.
Abstract
We report a new testing method named colloidal gold-immunochromatography assay (GICA) to detect syphilis instead of fluorescent treponemal antibody-absorption (FTA-Abs). Syphilis-specific immunoglobulin G (IgG) antibody was detected with GICA established on syphilis-specific recombinant proteins, TP...
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PMID: 20846810
PDF is available here.
PDF is available here.
Abstract
The ADVANCED QUALITY One Step Multi-Drug Screen test is a new urine on-site immunoassay test that is designed to detect multiple drugs of abuse in one time (barbiturates, benzodiazepines, cocaine, methylenedioxymethamphetamine (MDMA), methamphetamine, and opiates group). Thus, the present research w...
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PMID: 21114210
PDF is available here.
PDF is available here.
Abstract
In this study, a 10-channel up-converting phosphor technology-based lateral flow (TC-UPT-LF) assay was developed to profile antibodies against Yersinia pestis. Ten expressed Y. pestis proteins were covalently conjugated with an up-converting phosphor particle to develop double-antigen sandwich immun...
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PMID: 20801166
PDF is available here.
PDF is available here.
Abstract
In this paper, a sensitive immunoassay method was proposed for Listeria monocytogenes detection by using highly fluorescent bioconjugated nanoparticles probe. (FITC-IgG)-doped fluorescent silica nanoparticles (fsNPs) firstly were synthesized by a microemulsion method and characterized by TEM and flu...
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PMID: 20807555
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (135)11 2010
Competitive surface-enhanced Raman scattering assay for the 1,25-dihydroxy metabolite of vitamin D3.
Abstract
This paper describes the development and preliminary testing of a competitive surface-enhanced Raman scattering (SERS) immunoassay for calcitriol, the 1,25-dihydroxy metabolite (1,25-(OH)(2)-D(3)) of vitamin D(3). Deficiencies in 1,25-(OH)(2)-D have been linked to renal disease, while elevations are...
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PMID: 20830325
PDF is available here.
PDF is available here.
Abstract
We have developed an immunomicroarray for systematic studies of the binding properties of 10 different micro-/nano-sized streptavidin-functionalized beads to a biotin substrate immobilized on SiO(2) with or without surface modification. SiO(2) surface cleaning, immobilized substrate concentration an...
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PMID: 20417734
PDF is available here.
PDF is available here.
Biophysical Chemistry (151)3 2010
Abstract
The behaviour of binding reactions in immunoassays can be predicted and studied by modelling methods. Simple antibody-analyte binding reaction kinetics can be simulated by e.g. a mechanistic assay model based on differential equations. However, the mathematical modelling becomes more complicated if...
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PMID: 20580482
PDF is available here.
PDF is available here.
Brain and Cognition (74)1 2010
Abstract
In independent studies delirium was associated with higher levels of cortisol, interleukin(IL)s, and S100B. The aim of this study was to simultaneously compare cortisol, IL-6, IL-8, and S100B levels in patients aged 65years and older admitted for hip fracture surgery with and without delirium. Corti...
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PMID: 20580479
PDF is available here.
PDF is available here.
Anticancer Research (30)10 2010
Abstract
The clinical utility of tumour markers in pleural effusion is still controversial with regard to the efficient detection of malignant pleural mesothelioma (MPM).
The levels of carcinoembryonic antigen (CEA) and cytokeratin 19 fragment (CYFRA 21-1) were retrospectively studied in pleural effusion of...
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PMID: 21036761
PDF is available here.
PDF is available here.
Abstract
Young adults born very preterm show elevated adrenal androgens, particularly when born small for gestational age. Postnatal corticosteroid administration is positively associated with serum Adione in young women....
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PMID: 20682633
PDF is available here.
PDF is available here.
Abstract
The burden of childhood tuberculosis (TB) is influenced by the human immunodeficiency virus (HIV) epidemic and this dangerous synergy affects various aspects of both diseases; from pathogenesis and the epidemiologic profile to clinical presentation, diagnosis, treatment, and prevention. HIV-infected...
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PMID: 20651637
PDF is available here.
PDF is available here.
Abstract
More than 40 million people are affected by food-borne trematode infections. Diagnosis is unsatisfactory and there are only two drugs available for treatment and control: praziquantel and triclabendazole. This review provides an update on recent developments in the diagnosis and treatment of food-bo...
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PMID: 20683264
PDF is available here.
PDF is available here.
Lab On a Chip (10)18 2010
Abstract
This paper presents a rapid, ultra-low-cost approach to fabricate microfluidic devices using a polyolefin shrink film and a digital craft cutter. The shrinking process (with a 95% reduction in area) results in relatively uniform and consistent microfluidic channels with smooth surfaces, vertical sid...
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PMID: 20680207
PDF is available here.
PDF is available here.
Abstract
We verified the reliability of FT4 results by 3 commercial IAs throughout pregnancy, by comparison of the pattern to that obtained with an equilibrium dialysis isotope dilution-mass spectrometry (ED ID-MS) candidate reference measurement procedure.
Pregnant females (107) and age-matched non-pregnant...
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PMID: 20510682
PDF is available here.
PDF is available here.
Brain research (1350) 2010
Abstract
Second-generation antipsychotics are widely used in the treatment of all forms of psychoses, but they often produce undesirable side effects, among which are weight gain and other elements of metabolic syndrome. The mechanisms of these adverse effects are not known. The liver and adipose tissue are...
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PMID: 20570665
PDF is available here.
PDF is available here.
Acta Tropica (115)3 2010
Abstract
Recent studies suggest excretory-secretory (ES) antigen specific antibody detection tests to be of promising utility in laboratory diagnosis of many parasitic diseases in human including neurocysticercosis (NCC). The objective of the present study was to characterize the ES antigens collected from i...
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PMID: 20399738
PDF is available here.
PDF is available here.
Abstract
This paper reports on an inkjet printing method for the fabrication of lateral flow immunochromatographic devices made from a single piece of filter paper by patterning microfluidic channels and dispensing immunosensing inks, requiring only a single printing apparatus. This "paperfluidic" immunosens...
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PMID: 20652543
PDF is available here.
PDF is available here.
Abstract
A new detection format for multiplexed analysis based on fluorescent encoded magnetic composite nanoparticles is presented. Two kinds of virus were analyzed by this new method: equine influenza virus (EIV) and equine infectious anemia virus (EIAV). Firstly, EIV antigen and EIAV antigen were conjugat...
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PMID: 20652548
PDF is available here.
PDF is available here.
Abstract
The Chagas disease particle gel immunoassay (PaGIA-Chagas) is a simple, fast and practical test used for the diagnosis of the chronic Chagas disease and is based on anti-Trypanosoma cruzi antibody detection in serum. This study aimed to validate the PaGIA-Chagas on plasma collected with capillary tu...
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PMID: 21045375
PDF is available here.
PDF is available here.
Bioanalysis (2)9 2010
Abstract
We describe the development and preclinical validation of a highly sensitive Luminex(®) assay specific to IL-23 that is suitable for its measurement in support of early-phase clinical trials.
Intra-assay precision for the BioSource™ ELISA was under 12.3%, and under 5.2% for the eB...
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PMID: 21083285
PDF is available here.
PDF is available here.
Biomarkers (15)6 2010
Abstract
We tested the hypothesis that plasma concentration of matrix metalloproteinase (MMP)-2, tissue inhibitor of matrix metalloproteinases (TIMP)-4 and tenascin C (TNC) might be useful as biomarkers for assessing the severity of PH. Therefore, the concentrations of MMP-2, TIMP-4, TNC and N-terminal b-typ...
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PMID: 20528622
PDF is available here.
PDF is available here.
Abstract
Autoantibodies against thyroxin (T4AA) and triiodothyronine (T3AA) are present in dogs with autoimmune thyroiditis and have been reported to interfere with immunoassays. The objectives of this study were to determine the frequency of autoantibodies and to determine whether interference occurs by T4A...
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PMID: 20706025
PDF is available here.
PDF is available here.