Abstract
A one-step homogeneous colorimetric immunoassay format coupled with zeta potential measurements for determination of specific diabetic biomarker glycated hemoglobin (HbA1c) using functionalised gold nanoparticles as bioprobes is reported. The assay exhibited an excellent sensitivity based on absorba...
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PMID: 20571696
PDF is available here.
Abstract
A simple and sensitive immunosensor for the detection of cancer biomarker prostate specific antigen (PSA) has been developed. Around the percolation threshold of the graphene film, the conductivity of the graphene film varies significantly with the surface adsorption of molecules, which can be used...
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PMID: 20585690
PDF is available here.
Abstract
The introduction into routine diagnostic laboratories of solid phase assays for human leukocyte antigen (HLA) antibody detection has resulted in the application of new laboratory matching algorithms in clinical organ transplantation which have improved pre-transplant detection of immunization, in tu...
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PMID: 20403141
PDF is available here.
Abstract
Soy supplementation reduced total and subcutaneous abdominal fat and interleukin-6. No difference between groups was noted for glucose metabolism, C-reactive protein, tumor necrosis factor-alpha, leptin, or adiponectin. CONCLUSION: Soy supplementation reduced abdominal fat in obese postmenopausal wo...
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PMID: 20435291
PDF is available here.
Abstract
Human cytomegalovirus (HCMV)-specific CD4(+) and CD8(+) T-cells were measured in the immunocompetent host as well as in 13 solid-organ transplant recipients (SOTR), and 12 young hematopoietic stem cell transplant recipients (HSCTR) by using a long-term (7-day) assay based on PBMC stimulation by HCMV...
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PMID: 20493777
PDF is available here.
Abstract
We evaluated two new, rapid, automated, semi-quantitative chemiluminescent immunoassays in HIT suspected patients: HemosIL AcuStar HIT-IgG(PF4-H) (specific for IgG anti-PF4/heparin antibodies) and HemosIL AcuStar HIT-Ab(PF4-H) (detecting IgG, IgM and IgA anti-PF4/heparin antibodies) (both from Instr...
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PMID: 20539902
PDF is available here.
Abstract
Data on immune responses of young children using ATP release-based Cylex assay are insufficient. This study measured the immune response of healthy children less than three years of age to mitogens, PHA and Con-A. Blood was obtained from children attending routine health care visits. The Cylex assay...
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PMID: 20345614
PDF is available here.
Abstract
The measurement of antiepileptic drugs (AEDs) in different samples has received considerable attention due to the directly correlation between clinical effects and plasma concentration. Numerous methods have been extensively applied to the analysis of AEDs since many years ago providing reliable and...
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PMID: 20402636
PDF is available here.
Abstract
Acute kidney injury (AKI) following cardiac surgery with cardiopulmonary bypass (CPB) causes increased morbidity and mortality.
To evaluate the plasma profile of biomarkers potentially involved in AKI development following CPB.
In a nested case-control study, plasma levels of 27 biomarkers in 11 AKI...
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PMID: 20482449
PDF is available here.
Abstract
We therefore explored whether a missense mutation (rs1042044) in the GLP-1 receptor was associated with morning and evening salivary cortisol levels in preschool aged children. Morning and evening saliva samples and individual buccal swabs for DNA extraction were collected from seventy-seven prescho...
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PMID: 20483198
PDF is available here.
Abstract
We investigated human lipoprotein-associated neutral glycosphingolipids (GSLs) with emphasis on high (globotriaosylceramide) and low (globotetraosylceramide) affinity Stx-receptors. TLC overlay employing Stx1, Stx2, and anti-GSL antibodies demonstrated preferential distribution of globo-series GSLs...
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PMID: 20444989
PDF is available here.
Abstract
We describe the optimization of a protocol for extracting proteins from human tissues in a format that is compatible with the functional analysis of human T cells. The tissue was homogenized in a mixture of butan-1-ol, acetonitrile and water and then lyophilized. Lyophilized protein extracts were di...
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PMID: 20470778
PDF is available here.
Abstract
Luminescent samarium (Sm) and europium (Eu) doped silica nanoparticles (SiO(2) NPs) were investigated for use in multiple target immunoassays. Particles with a diameter of 40 +/- 10 nm were synthesized and applied to multiplexed immunoassays in a single well of an assay plate. The luminescent behavi...
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PMID: 20571623
PDF is available here.
Abstract
A new strategy was described for amplifying the response of the antigen-antibody sensing processes by functionalizing SiO(2) nanoparticles labeled secondary antibodies based on a sandwich immunoassay in this work. At first, the multi-walled carbon nanotubes (CNTs) were individually dispersed in the...
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PMID: 20523934
PDF is available here.
Abstract
These results demonstrate the high sensitivity achieved with this immunosensor design which compares favourably with other previous reports. The immunosensor also exhibited a high selectivity with respect to other corticosteroid compounds closely related to cortisol. The usefulness of the immunosens...
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PMID: 20577675
PDF is available here.
Abstract
This RCT provides no evidence for treating elderly subjects with SCH with T4 replacement therapy to improve cognitive function....
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PMID: 20501682
PDF is available here.
Abstract
We developed a simple, rapid and inexpensive one-step immunomagnetic method for separating and counting CD4(+) T lymphocytes on microfluidic devices with enlarged reaction chambers. CD4(+) T lymphocytes were successfully separated and captured from the cell suspension obtained from mouse thymus. CD4...
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PMID: 20602931
PDF is available here.
Abstract
We compared an automated quantitative heart-type fatty acid-binding protein (H-FABP) assay with other cardiac-marker assays to examine its usefulness as an early diagnostic marker of acute myocardial infarction (AMI). Serum samples for cardiac troponin T (cTnT), creatine kinase-MB isozyme (CK-MB), m...
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PMID: 20551280
PDF is available here.
Abstract
We studied the level of ASO (anti streptolysin O) titer and throat culture for GABHS (group A beta hemolytic streptococci) in 200 normal healthy school children between 5 to 15 years of age. We noted the upper limit of normal titer of 242 IU in the healthy school children around Mysore city. Throat...
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PMID: 20683117
PDF is available here.
Abstract
Study patients were divided into two groups: 61 patients with bacterial infection and 26 patients without it. Semi-quantitative PCT test (cut-off value; > or = 0.5 ng/ml) showed sensitivity of 55.7% and specificity of 84.6% for diagnosis of bacterial infection. The diagnostic value of PCT was higher...
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PMID: 20715510
PDF is available here.
Abstract
These data can be useful for optimizing the immunization strategy against varicella and should be confirmed by a prospective study including a large number of immunized infants.
Copyright (c) 2010 The British Infection Society. Published by Elsevier Ltd. All rights reserved....
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PMID: 20380851
PDF is available here.
Abstract
BACKGROUND: Newborn screening is a state-based public health program established as a means for the early detection and treatment of certain medical conditions to minimize developmental disability and mortality. The program was initiated more than 40 years ago to detect and prevent phenylketonuria....
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PMID: 20489136
PDF is available here.
Abstract
n 2005, CDC published guidelines for using the QuantiFERON-TB Gold test (QFT-G) (Cellestis Limited, Carnegie, Victoria, Australia) (CDC. Guidelines for using the QuantiFERON-TB Gold test for detecting Mycobacterium tuberculosis infection, United States. MMWR;54[No. RR-15]:49-55). Subsequently, two n...
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PMID: 20577159
PDF is available here.