Abstract
We were able to determine the cutoff value for the HRT threshold in relation to outcomes of OFCs. The cutoff value was 6 ng/ml of egg white antigen in HEA and REA (p < 0.01), 40 ng/ml of milk antigen in CMA (p < 0.01), and 500 ng/ml of wheat antigen in WA (p < 0.05). The efficiency was 70.3% for HEA...
|
PMID: 21646803
PDF is available here.
Abstract
Skin flaps were characterized by the necrotic loss of tissue starting from distal areas of the flaps in diabetic mice. Decay of epidermal and dermal structures within skin flap tissue was paralleled by an immune cell-mediated expression of chemokines (macrophage inflammatory protein 2, macrophage ch...
|
PMID: 20623767
PDF is available here.
Abstract
After extensive discussion with the Fukuoka City government of measures for the prevention and control of the 2009 H1N1 influenza pandemic, Kyushu University Hospital organized the infection control teams of 39 hospitals in the Fukuoka City area in preparation for a possible outbreak. A facility was...
|
PMID: 20845752
PDF is available here.
Abstract
Point-of-care diagnosis of influenza requires sound recognition of the prevalence of the signs and symptoms of the patients, which would help to define the pattern of clinical presentation of this infection. In severely ill patients, however, clinical features of influenza could be erroneously modif...
|
PMID: 20845739
PDF is available here.
Abstract
The impact of autoimmune diseases is growing from both a clinical and a laboratory point of view. Diagnostic assays are now being transferred from dedicated specialised laboratories into high-throughput service laboratories. The increasing number of available methods has raised the variability among...
|
PMID: 20511607
PDF is available here.
Abstract
Colorectal cancer is one of the most frequent malignant neoplasms which affects humans. Last year studies indicate a constantly increasing inception rate. Multidisciplinary teams direct all their efforts towards detection of cancer in it's asymptomatic phase. In parallel with development of diagnost...
|
PMID: 20842828
PDF is available here.
Abstract
Mean immune response in 61 samples was 368 +/- 179 ATP ng/ml. Immune response was lower during BK virus (BKV) or CMV viremia compared to no viremia (p = 0.009 and p = 0.017), and no viremia was seen if immune response was > 380. BK or JC viruria was not associated with low immune response. Immune re...
|
PMID: 20630132
PDF is available here.
Abstract
The need to verify sensitization to mycotic allergens in children with allergic bronchopulmonary diseases is due to the severity of their clinical course, the high frequency of complications, and the inadequate efficiency of conventional treatment regimens. The sensitivity of most techniques used in...
|
PMID: 20886720
PDF is available here.
José Raúl García-Lozano,
Cristina Abad,
Ana Escalera,
Belén Torres,
Olga Fernández,
Alicia García,
Julio Sánchez-Román,
José-Mario Sabio,
Norberto Ortego-Centeno,
Enrique Raya-Alvarez,
Antonio Núñez-Roldán,
Javier Martín and
María Francisca González-Escribano
Abstract
We defined four major haplotypes in our population: the two major haplotypes (named haplotypes A and B) bear both the 5383_5397del variant and the two most common SNP sets found in the CEU population. Quantification analysis revealed that genotype B/B had the highest median of mRNA expression levels...
|
PMID: 20549518
PDF is available here.
Abstract
We applied the QFT-2G tests to numerous contact investigations and found that the majority of contacts who had been supposed to be infected with M. tuberculosis (Mtb) based on TST were negative in the QFT-2G tests, strongly suggesting the possibility that the unnecessary preventive chemotheraphy was...
|
PMID: 20715545
PDF is available here.
Abstract
Hospital acquired infection mainly occurs at hospitals, not clinics. In Japan, QFT-G is the main tool for the diagnosis of tuberculous infection among health care workers. Contact examinations are basically done for contacts of sputum smear positive TB cases, but infection may occur at fiberbronchos...
|
PMID: 20715546
PDF is available here.
Abstract
We aim to give an overview about the generation, structure, properties of IgY, as well as the advantages of chicken antibodies use over mammalian antibodies in immunodiagnostics and immunotherapy.
Copyright 2010 Elsevier B.V. All rights reserved....
|
PMID: 20083313
PDF is available here.
Abstract
We can consider the use of immunotherapy in women with immune causes of reproductive failure. This review looks at the current available information on the immunology surrounding reproductive failure and the current therapies offered to rectify these abnormalities. Till date numerous studies have lo...
|
PMID: 20595949
PDF is available here.
Abstract
The standard in vitro assays used for the generation and characterization of antibodies that bind and neutralize the influenza virus have not been modified significantly in many years. The use of cultured human cells has been instrumental in understanding the basis of the immune response, and the in...
|
PMID: 20373254
PDF is available here.
Abstract
We investigated the role of auto-antibodies as detected by different serological assays. Nine reports containing data on 143 patients were analyzed. Pre-treatment data showed that indirect immunofluorescence, enzyme-linked immunosorbent assay (ELISA) and immunoblotting offer an 82.2% or greater prob...
|
PMID: 20169293
PDF is available here.
Abstract
Identification of Mycobacterium tuberculosis antigens inducing cellular immune responses is required to improve the diagnosis of and vaccine development against tuberculosis. To identify the antigens of M. tuberculosis that differentiated between tuberculosis (TB) patients and healthy contacts based...
|
PMID: 20031926
PDF is available here.
Abstract
Evidence regarding IGRA performance in children is accumulating rapidly. Overall, the findings demonstrate performance of IGRAs equivalent or superior to that of the TST. However, IGRAs have biological limitations similar to TST and some technical problems of their own, and critical gaps in our know...
|
PMID: 19952926
PDF is available here.
Abstract
Compared with the absence of screening and with G-FOBT, the biennial two-stool immunochemical test can be considered a promising method for mass screening for colorectal cancer....
|
PMID: 20059779
PDF is available here.
Abstract
Chikungunya (CHIK) is a re-emerging disease causing a large negative impact on global health and economics. Clinical manifestations of CHIK are non specific and difficult to differentiate from dengue hemorrhagic fever or other viral exanthema. A rapid, simple and reliable diagnostic assay is necessa...
|
PMID: 20578487
PDF is available here.
Abstract
Human visceral leishmaniasis is a serious public health problem in endemic countries because of its high potential lethality, particularly in children. Rapid diagnosis is essential to early treatment and control of visceral leishmaniasis.
|
PMID: 20890548
PDF is available here.
Abstract
Four N-terminal His6-tagged recombinant fragments of CagA protein were produced. Protein rCagAfr.1 (65 kDa) represents the most conserved N-terminal part of the cytotoxin. Fragment rCagAfr.2 (44 kDa) corresponds to the central conserved region of CagA whereas rCagAfr.3 (39 kDa) represents the highly...
|
PMID: 20465006
PDF is available here.
Abstract
Food-dependent exercise-induced anaphylaxis (FDEIA) is a special form of food allergy where a food-intake alone does not induce any symptoms. However, allergic symptoms are elicited when triggering factors such as exercise or aspirin-intake are added after ingestion of the causative food. The most f...
|
PMID: 19847096
PDF is available here.
Abstract
We found a relationship between the probability of failed challenge and the concentration of IgE antibodies to both wheat and soybean. Age influences the relationship of allergen specific IgE levels to wheat and oral food challenge outcome. Younger children are more likely to react to low levels of...
|
PMID: 19776678
PDF is available here.
Abstract
Diagnosis of food allergy should be based on the observation of allergic symptoms after intake of the suspected food. The oral food challenge test (OFC) is the most reliable clinical procedure for diagnosing food allergy. The OFC is also applied for the diagnosis of tolerance of food allergy. The Ja...
|
PMID: 19847093
PDF is available here.
Abstract
Unwanted immunogenicity, i.e., the developpement by patients of anti-drug antibodies is a significant problem with biologicals therapeutic reagents and can compromise clinical response. Over 20 antibodies currently on the market and over 100 drug candidates are currently in clinical trials; all ther...
|
PMID: 20035681
PDF is available here.
Abstract
Dogs and cats are commonly exposed to infectious disease agents. The following article is an update on the most common infectious disease assays used in small animal practice. In some situations, results of pathogen-specific molecular diagnostic assays can be used to assist the pract...
|
PMID: 19945088
PDF is available here.
Abstract
Colorectal cancer (CRC) can be prevented by screening programs in the population at average risk (men and women aged between 50 and 74 years) and at high risk (first degree relatives, CRC hereditary syndromes and chronic inflammatory bowel disease). Early CRC (with submucosal invasion) and advanced...
|
PMID: 19577340
PDF is available here.
Abstract
The comparison between the Vitek Immunodiagnostic Assay System (VIDAS) and PCR methods for the detection of the pathogenic microorganisms Salmonella Typhimurium, Listeria monocytogenes, Escherichia coli O157:H7, Clostridium perfringens, and Staphylococcus aureus in salchich6n (a type of Spanish ferm...
|
PMID: 19777902
PDF is available here.
Abstract
Provocation tests are regarded as the "gold standard" to establish or exclude the presence of hypersensitivity to a certain drugs because they reproduce not only allergy symptoms but other adverse manifestations, irrespective of their pathomechanism. Provocation testing is potentially harmful and sh...
|
PMID: 19563998
PDF is available here.
Abstract
The diagnosis of a drug hypersensitivity reaction (DHR) is a challenging task because multiple and complex mechanisms are involved. Better understanding of immunologic pathomechanisms in DHRs and rapid progress in cellular-based in-vitro tests can help to adjust the correct diagnostic strategy to in...
|
PMID: 19563996
PDF is available here.
Abstract
We describe the structure, selection and production of single-chain antibodies. Furthermore, we review current applications of antibody fragments focusing on thrombus targeting using fibrin- and platelet-specific single-chain antibodies as well as describing novel noninvasive imaging approaches for...
|
PMID: 19492141
PDF is available here.
Abstract
In this article review diagnostic value of protein group-lectins for practical oncology are presented. It is indicated, that with some carbohydrate structures lectins revealed the possibility of reversible and selective action and with earlier elaborated methods, which are based on identification of...
|
PMID: 19556645
PDF is available here.
Abstract
Although the IGRA, compared with the tuberculin skin test (TST), has greater specificity in BCG-vaccinated individuals, treatment of latent tuberculosis infection is not a priority in high-burden setting. Nevertheless, in high-burden settings, the TST performs reasonably well and correlates as well,...
|
PMID: 19387262
PDF is available here.
Chang-rong CR Chen,
Quan Q Yuan,
Sheng-xiang SX Ge,
Shan-hai SH Ou,
Hong-ying HY Ni,
Yong-chang YC Zhang,
Bin B Pei,
An A Li,
Qiang Q Yan,
Yong-cai YC Lin,
Qing-rui QR Chen,
Ning-shao NS Xia and
Jun J Zhang
Abstract
Occult hepatitis B virus (HBV) infection status of blood donors in a southern city in China was investigated by immunological assays and nucleic acid testing. Overall, 17 (0.19%, 95% CI: 0.11%-0.30%) of the 9023 HBsAg negative samples were found to be positive for the presence of HBV DNA. "A" epitop...
|
PMID: 19634759
PDF is available here.
U Mack,
G B Migliori,
M Sester,
H L Rieder,
S Ehlers,
D Goletti,
A Bossink,
K Magdorf,
C Hölscher,
B Kampmann,
S M Arend,
A Detjen,
G Bothamley,
J P Zellweger,
H Milburn,
R Diel,
P Ravn,
F Cobelens,
P J Cardona,
B Kan,
I Solovic,
R Duarte,
D M Cirillo,
C. Lange and
TBNET
Abstract
We were to gain a better understanding on M. tuberculosis latency and reactivation. This statement by the TBNET summarises knowledge and limitations of the currently available tests used in adults and children for the diagnosis of latent tuberculosis infection. In summary, the main issue regarding t...
|
PMID: 19407047
PDF is available here.
Abstract
The ELISPOT assay detecting MTB-specific immune response is promising both in diagnosing MTB and monitoring responsiveness to tuberculosis therapy....
|
PMID: 19522306
PDF is available here.
Abstract
Evidence from several studies supports that the epigenetic, transcriptional and translational regulation and expression of O6-methylguanine-methyltransferase (MGMT) is relevant for prognostic and predictive considerations in glioblastoma patients. MGMT status is being used as a stratifying factor or...
|
PMID: 19224454
PDF is available here.
Abstract
We have shown that the IgE measurement induced by systemic exposure of BALB/c mice to a range of proteins correlates broadly with what is known of their allergenic potential in humans. The approach used a homologous passive cutaneous anaphylaxis (PCA) assay that reflects IgE-dependent biological act...
|
PMID: 18937215
PDF is available here.
Abstract
Detection the Anti-dsDNA antibody of the serum sample with the methods of IIF, ELISA and Farr. The positive percentage of Anti-dsDNA in SLE is 25%, 32% and 32%, while in RA is 0, 0 and 0; in PSS is 0, 0 and 5%; in SS is 0, 0 and 0; in healthy is 0, 0 and 0. CONCLUSION: Detection the Anti-dsDNA antib...
|
PMID: 19799026
PDF is available here.
Abstract
We describe data collected by using high-throughput techniques such as antigen microarrays and mass spectrometry for antibody profiling. While recently collected data demonstrate satisfactory analytical sensitivity and reproducibility, some issues such as standardization and data interpretation have...
|
PMID: 18718878
PDF is available here.
Abstract
An overview is given on recent trends and applications of rapid immunodiagnostic tests for screening of food and feed for mycotoxins. Different test formats are discussed, and challenges in the development of lateral-flow devices for on-site determination of mycotoxins, with requirements such as bei...
|
PMID: 18936919
PDF is available here.
Jose J Domínguez,
Malú M De Souza-Galvão,
Juan J Ruiz-Manzano,
Irene I Latorre,
Cristina C Prat,
Alicia A Lacoma,
Celia C Milà,
Maria Angeles MA Jiménez,
Silvia S Blanco,
José J Maldonado,
Neus N Altet and
Vicente V Ausina
Abstract
The objectives of the study were to assess the performance of the QuantiFERON-TB Gold In-Tube (QFN-G-IT) and the T-SPOT.TB tests in the immunodiagnosis of active tuberculosis (TB) in adult patients, and to study the T-cell interferon gamma (IFN-gamma) responses during treatment and in patients who h...
|
PMID: 19026511
PDF is available here.
Abstract
In modern society, there is a rise in the incidence of tuberculosis in all age groups, including children and adolescents. In old age group, a specific inflammation is detectable from Mantoux test results only in every four children. Tuberculous infection is diagnosed in half of cases when they turn...
|
PMID: 19253683
PDF is available here.
Abstract
The data of a comprehensive study of 86 children aged 6 to 14 years, who were examined and treated at the Research Institute of Phthisiology for various manifestations of tuberculous infection: 25.6% with infected Mycobacterium tuberculosis with varying specific sensitization; 34.9% with minor forms...
|
PMID: 19253682
PDF is available here.
Abstract
These results indicate that the lack of reports should not be the only criterion for an area to be considered as Trichinella-free. The awareness of the existence of the parasite in a region will favor the establishment of control and prevention strategies which is of fundamental importance to avoid...
|
PMID: 19622475
PDF is available here.
Abstract
There was a significant relationship of the levels of Ab to Cat and XO to the activity of SLE. It was shown that Ab to Cat and XO could affect the functional activity of serum enzymes. The level of Ab to CL in patients with SLE was found to depend on two parameters - the intensity of the disease and...
|
PMID: 20481049
PDF is available here.
Abstract
CIC levels, serum aDNA, IL-1beta and IL-4, oxygen-dependent neutrophil metabolism were higher both in SLE and ILE. Only SLE was characterized by high oxygen stress of monocytes, acid phosphatase and cation proteins in neutrophils and monocytes. Lymphocyte phenotype both in SLE and ILE was characteri...
|
PMID: 19514420
PDF is available here.
Abstract
Determination of plasma IL-4 and IL-5 levels can be a useful tool for evaluation of the effects of food challenge tests on children with asthma and coexisting IgE-dependent food allergy. The results of determining serum IL-10, TNF-alpha, interleukin (IL) IFN-gamma, sIL-2R, and sCD23 levels during th...
|
PMID: 19274928
PDF is available here.
Abstract
Drug provocation tests in children are always a problematic task. In the present article the most important aspects of this technique are reviewed, including the differences between children and adults; the main mechanisms involved in drug reaction; how to perform the different tests; and when they...
|
PMID: 19945774
PDF is available here.