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Embryonic Structures (24)
- Branchial Region
- Cleavage Stage, Ovum
- Embryo, Mammalian
- Embryo, Nonmammalian
- Germ Layers
- Limb Buds
- Mullerian Ducts
Articles on Embryonic Structures
The activity of Pax3 and Zic1 regulates three distinct cell fates at the neural plate border.
We have analyzed the role of two transcription factors, Pax3 and Zic1, which are among the earliest genes activated in response to neural plate border-inducing signals. At the end of gastrulation, Pax3 and Zic1 are coexpressed in the neural crest forming region. In addition, Pax3 is expressed in pro...
Rapid Evaluation of Soluble HLA-G Levels in Supernatants of In Vitro Fertilized Embryos
We established a rapid detection assay based on Luminex technology, which can be integrated into ART proceedings, allowing sHLA-G quantification in sample volumes of only 10 microl within 1.5 hours. Using this method, sHLA-G levels of 526 single-embryo cultures, 47 two-embryo cultures, and 15 three-...
Metalloproteinase axes increase beta-catenin signaling in primary mouse mammary epithelial cells lacking TIMP3.
We demonstrate that primary mouse embryonic fibroblasts (MEFs) and mammary epithelial cells (MECs) deficient in Timp3 have increased beta-catenin signaling. Functionally, the loss of TIMP3 exerted cell-type-specific effects, with Timp3(-/-) MEFs being more sensitive and Timp3(-/-) MECs more resistan...
Ciliation and gene expression distinguish between node and posterior notochord in the mammalian embryo
We find that Hensen's node in the rabbit is devoid of cilia, and that ciliated cells are restricted to the notochordal plate, which emerges from the node rostrally. In a comparative approach, we use the organizer marker gene Goosecoid (Gsc) to show that a region of densely packed epithelium-like cel...
Interpopulation variation in life-history traits of Poeciliopsis prolifica: implications for the study of placental evolution.
We studied variation in life-history traits in two populations of the placental poeciliid fish Poeciliopsis prolifica to determine whether differences in post-fertilization maternal provisioning to embryos have a genetic basis and how food availability affects reproduction. Life histories were chara...
Retinoic acid is required for specification of the ventral eye field and for Rathke's pouch in the avian embryo.
We have investigated the role of retinoic acid (RA) in eye development using the vitamin A deficient quail model system, which overcomes problems of retinoic acid synthesising enzyme redundancy in the embryo. In the absence of retinoic acid, the ventral optic stalk and ventral retina are missing, wh...
Asymmetric cell divisions in the early embryo of the leech Helobdella robusta.
We review the early development of Helobdella, focusing on the variety of unequal cell divisions. We then summarize an experimental analysis of the mechanisms underlying the unequal first cleavage in Helobdella, concluding that the unequal first cleavages in Helobdella and Tubifex proceed by differe...
Establishment of three-dimensional culture of neural stem/progenitor cells in collagen Type-1 Gel.
In viability assay, the viability rate increased as the NSPC density increased, and peaked at 1 x 10(7) to 5 x 10(7) cell/ml. For collagen concentration, the viability rate increased as the collagen concentration decreased. In migration assay, cell migration was most extensive at collagen concentrat...
Heterotopic, not homotopic, fetal occipital allografts in adult hosts project to visual-related extracortical targets.
These puzzling results question the use of transplantation strategies for repairing damaged networks in adults where rewiring involves complex white matter trajectories....
Identification of the Drosophila skpA gene as a novel target of the transcription factor DREF
SKPa is component of a Drosophila SCF complex that functions in combination with the ubiquitin-conjugating enzyme UbcD1. skpA null mutation results in centrosome overduplication, unusual chromatin condensation, defective endoreduplication and cell-cycle progression. While the molecular mechanisms th...