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Recent article
Germ Layers
Abstract
We show sheep fibroblasts can be reprogrammed to pluripotency by defined factors using a drug-inducible system. Sheep iPSCs derived in this fashion have a normal karyotype, exhibit morphological features similar to those of human ESCs and express AP, Oct4, Sox2, Nanog and the cell surface marker SSE...
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PMID: 21253598
PDF is available here.
PDF is available here.
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Abstract
We have previously reported an engineered three-dimensional culture system for controlled ES cell-ES cell interaction via the avidin-biotin binding complex. Here we investigate the effect of such engineering on ES cell differentiation. Engineered EBs exhibit enhanced osteogenic differentiation asses...
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PMID: 19751101
PDF is available here.
PDF is available here.
Abstract
We demonstrate that Rac1 ablation in embryonic stem cell-derived embryoid bodies (EBs) leads to massive apoptosis of epiblast cells in contact with the BM. Expression of wild-type Rac1 in the mutant EBs rescues the BM-contacting epiblast, while expression of a constitutively active Rac1 additionally...
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PMID: 20457815
PDF is available here.
PDF is available here.
Development (137)14 2010
Abstract
We investigate whether EG cell self-renewal and derivation are supported by 2i. We report that mouse EG cells can be established with high efficiency using 2i in combination with the cytokine leukaemia inhibitory factor (LIF). Furthermore, addition of fibroblast growth factor or stem cell factor is...
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PMID: 20519324
PDF is available here.
PDF is available here.
Abstract
Embryonic stem (ES) cells have the potential to develop into any type of tissue and are considered as a promising source of seeding cells for tissue engineering and transplantation therapy. The main catalyst for ES cells differentiation is the growth into embryoid bodies (EBs), which...
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PMID: 20659709
PDF is available here.
PDF is available here.
Abstract
We present evidence for a novel histological and embryological relationship at the human materno-fetal interface. Here an epi- endo- thelium forms an integrated unicellular layer lining the intervillus space in between the anchoring villi that attach the placenta to the uterus. This layer appears to...
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PMID: 20236701
PDF is available here.
PDF is available here.
Abstract
OxF1 is a human embryonic stem cell line derived from a surplus embryo donated through the Oxford IVF clinic. The cells have a stable 46 XX karyotype and show expression of Oct 4, Nanog and TRA-1-60. Embryoid bodies differentiate into cells that represent all three germ layers as demonstrated by imm...
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PMID: 20333479
PDF is available here.
PDF is available here.
Abstract
We report here the legislative issues related to embryo research and human embryonic stem cell (hESC) research in Spain and the derivation of nine hESC lines at the Center of Regenerative Medicine in Barcelona. You can find the information for obtaining our lines for research purposes at blc@cmrb.eu...
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PMID: 20177997
PDF is available here.
PDF is available here.
Genome Research (20)2 2010
Abstract
We constructed genome-wide replication-timing profiles of 22 independent mouse cell lines representing 10 stages of early mouse development, and transcription profiles for seven of these stages. Replication profiles were cell-type specific, with 45% of the genome exhibiting significant changes at so...
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PMID: 19952138
PDF is available here.
PDF is available here.
Abstract
We describe the expression of two novel transcripts, Ende (AK014119) and Npe (AK084355), during early mouse embryogenesis. Ende mRNA was first detected at embryonic day (E) 7.0 in a small population of epiblast cells in the distal half of the embryo. At E7.5, Ende was expressed by newly formed defin...
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PMID: 20153842
PDF is available here.
PDF is available here.
Abstract
We also describe basic assays used to characterize new EpiSC lines....
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PMID: 20336514
PDF is available here.
PDF is available here.
Abstract
The aim of the present study was to characterise bovine epiblast-derived outgrowth colonies (OCs) with respect to the embryonic origin of their cellular components. Epiblasts were isolated mechanically from bovine Day 12 embryos. Epiblasts were cultured on feeder layers of SNL cells (neomycin-resist...
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PMID: 20353722
PDF is available here.
PDF is available here.
Abstract
Abstract
We analysed the function of key developmental growth factors such as Activin, FGF and BMP in the control of early cell fate decisions of human pluripotent stem cells. This analysis resulted in the development and validation of chemically defined culture conditions for achieving specification of huma...
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PMID: 19564924
PDF is available here.
PDF is available here.
Abstract
Pluripotent cells can be isolated from the mammalian inner cell mass (ICM) of the embryo at the blastocyst stage, and maintained in culture as undifferentiated, embryonic stem cells (ES). These cells are an important model of mammalian development in vitro and are the focus of a great deal of resear...
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PMID: 19757397
PDF is available here.
PDF is available here.
Developmental Biology (325)1 2009
Abstract
We show that depletion of maternal irf6 transcripts in X. laevis embryos leads to gastrulation defects and rupture of the superficial epithelium. These experiments reveal a conserved role for maternally-encoded Irf6 in differentiation of a simple epithelium in X. laevis and D. rerio. This epithelium...
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PMID: 19013452
PDF is available here.
PDF is available here.
Abstract
We have investigated potential differences between embryos of permissive and recalcitrant strains during diapause and ES cell derivation. We found that in diapause embryos of the recalcitrant C57BL/6 and CBA strains, the epiblast failed to expand during the primary explant phase of ES cell derivatio...
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PMID: 18837461
PDF is available here.
PDF is available here.
médecine/sciences (24)12 2008
Abstract
In mammals, embryonic and extraembryonic cell lineages segregate during the first steps of cell differentiation in the preimplantation embryo. Indeed, mammal embryos contain very low energy stocks and thus get ready for implantation very early to be able to absorb nutrients from the mother, first th...
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PMID: 19116112
PDF is available here.
PDF is available here.
Genetika (Moskva) (44)12 2008
Abstract
Embryonic stem cells are able to give rise after differentiation to derivatives of three germinal layers (ectoderm, endoderm, and mesoderm) and to functional gametes. This property of cells is referred to as pluripotency. The pluripotent status of preimplantation embryo cells and embryonic stem cell...
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PMID: 19178078
PDF is available here.
PDF is available here.
Cell Stem Cell (3)6 2008
Abstract
We employ a temporally restricted genetic tracing strategy to selectively label the endothelium, and separately its underlying mesenchyme, during AGM development. Lineage tracing endothelium, via an inducible VE-cadherin Cre line, reveals that the endothelium is capable of HSC emergence. The endothe...
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PMID: 19041779
PDF is available here.
PDF is available here.
Cell Stem Cell (3)6 2008
Abstract
We generated monkey iPS cells by retrovirus-mediated introduction of monkey transcription factors OCT4, SOX2, KLF4, and c-MYC....
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PMID: 19041774
PDF is available here.
PDF is available here.
Journal of Neuroscience (28)42 2008
Abstract
We report that mice with epiblast-specific loss of dystroglycan develop brain and eye defects that broadly resemble the clinical spectrum of the human disease, including aberrant neuron migration, hydrocephalus, and malformations of the anterior and posterior chambers of the eye. Breaches of basemen...
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PMID: 18923033
PDF is available here.
PDF is available here.
Genes & Development (22)17 2008
Abstract
We report on the identification of the RNA-binding protein XSeb4R as a positive regulator of VegT. XSeb4R interacts directly with the 3'-untranslated region of VegT mRNA, stabilizes it, and stimulates translation. Ablation of XSeb4R activity results in impairment of endoderm and mesoderm formation,...
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PMID: 18765788
PDF is available here.
PDF is available here.
Abstract
We have devised a strategy for isolating cells from diverse embryonic regions and stages in the zebrafish, using computer-guided laser photoconversion of injected Kaede protein and flow cytometry. This strategy enabled us to perform a genome-wide transcriptome comparison of germ layer precursor cell...
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PMID: 18719100
PDF is available here.
PDF is available here.
Cardiovascular Research (79)3 2008
Abstract
A Cited2 conditional allele enabled its deletion in particular cell lineages in mouse development. A lacZ reporter cassette allowed indication of deletion. Congenic Cited2 heterozygous mice were used to investigate haploinsufficiency. Embryos were examined by magnetic resonance imaging, by sectionin...
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PMID: 18440989
PDF is available here.
PDF is available here.
Current Biology (18)14 2008
Abstract
During vertebrate gastrulation, cells separate into different germ layers. A recent study investigates and quantifies the roles of cell adhesion and cortical tension during germ layer formation in zebrafish.
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PMID: 18644340
PDF is available here.
PDF is available here.
Abstract
We focus on the role of Notch signaling in governing cell-fate choices in human embryonic stem cells (hESCs). Using genetic and pharmacological approaches, we achieved both blockade and conditional activation of Notch signaling in several hESC lines. We report here that activation of Notch signaling...
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PMID: 18462696
PDF is available here.
PDF is available here.
Abstract
Our studies show that ablation of BAF250a in early mouse embryos results in developmental arrest (about embryonic day 6.5) and absence of the mesodermal layer, indicating its critical role in early germ-layer formation. Moreover, BAF250a deficiency compromises ES cell pluripotency, severely inhibits...
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PMID: 18448678
PDF is available here.
PDF is available here.
Differentiation (76)5 2008
Abstract
We analyzed the expression of the protein during early embryonic development and organogenesis of the mouse. The results showed differential tissue-specific regulation of CD2AP in developing and maturing organs. In oocytes and pre-implantation embryos, CD2AP was located diffusely in the cytoplasm, w...
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PMID: 18177421
PDF is available here.
PDF is available here.
Nature Cell Biology (10)4 2008
Abstract
We use atomic force microscopy (AFM) to quantify the adhesive and mechanical properties of individual ectoderm, mesoderm and endoderm progenitor cells from gastrulating zebrafish embryos. Combining these data with tissue self-assembly in vitro and the sorting behaviour of progenitors in vivo, we hav...
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PMID: 18364700
PDF is available here.
PDF is available here.
Acta Tropica (105)3 2008
Abstract
"Anomalous", as yet unreported forms of brood capsules (BC) in cystic echinococcosis larvae from humans and sheep are described, thought to be the likely effect of degenerative involution of the parasite. Morphological examination showed an inner laminated layer (LL) in the brood capsules in intimat...
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PMID: 18243150
PDF is available here.
PDF is available here.
Abstract
Our results suggested that expression of pluripotency markers in undifferentiated pig epiblast cells more closely resembled that observed in hESC. Expression alterations of ESC-associated factors in epiblast cells were also observed during in vitro culture. Our data demonstrate the potential use of...
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PMID: 17680630
PDF is available here.
PDF is available here.
Abstract
We investigated the role of fibroblast growth factor-8 (FGF8) in the expression of SMA. In situ hybridization and reverse transcription-polymerase chain reaction showed that Fgf8b is expressed predominantly in the nascent hypoblast. Anti-FGF8b antibody inhibited the expression of SMA, cTNT, and Tbx5...
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PMID: 18312428
PDF is available here.
PDF is available here.
Journal of Cell Biology (180)3 2008
Abstract
We address the mechanisms underlying generation of skeletal muscle, smooth muscle, and endothelium from epithelial progenitors in the dermomyotome. Lineage analysis shows that of all epithelial domains, the lateral region is the most prolific producer of smooth muscle and endothelium. Importantly, i...
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PMID: 18268106
PDF is available here.
PDF is available here.
Stem Cells (26)2 2008
Activin alters the kinetics of endoderm induction in embryonic stem cells cultured on collagen gels.
Abstract
We describe a novel approach to induce endoderm from mouse embryonic stem (mES) cells using fibronectin-coated collagen gels. This technique results in a homogeneous endoderm-like cell population, demonstrating endoderm-specific gene and protein expression, which remains committed following in vivo...
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PMID: 18065398
PDF is available here.
PDF is available here.
Development (135)3 2008
Abstract
We have used a novel Eomes conditional allele to test Eomes functions in the embryo proper. Eomes-deficient embryos express both Fgf8 and its downstream target Snail at normal levels but surprisingly fail to downregulate E-cadherin. Eomes functional loss thus efficiently and profoundly blocks EMT an...
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PMID: 18171685
PDF is available here.
PDF is available here.
Reproductive Toxicology (25)1 2008
Abstract
Although L-CAM is distributed normally after Cd, post-translational modification may occur causing breakdown of its normal association with the catenins and actin, and allowing beta-catenin to translocate to the nucleus in peri-ectodermal tissue, mimicking the canonical Wnt pathway....
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PMID: 18031986
PDF is available here.
PDF is available here.
Immunology (26) 2008
Abstract
The epithelial architecture of the thymus fosters growth, differentiation, and T cell receptor repertoire selection of large numbers of immature T cells that continuously feed the mature peripheral T cell pool. Failure to build or to maintain a proper thymus structure can lead to defects ranging fro...
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PMID: 18304000
PDF is available here.
PDF is available here.
Abstract
We provide a brief overview of grid-free methods for modeling multicellular systems. We focus on an approach based on Langevin equations, in which inertia is ignored, and stochastic effects on cell motion are included. The discussion starts with simpler models, in which cells are modeled as adhesive...
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PMID: 18023727
PDF is available here.
PDF is available here.
Abstract
Developmental Biology (312)1 2007
Abstract
We demonstrate that embryos lacking the signaling factor Wnt3 exhibit defects in this axial realignment. In addition, chimeric analyses and conditional removal of Wnt3 activity reveal that Wnt3 expression in the epiblast is required for induction of the primitive streak and mesoderm whereas activity...
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PMID: 18028899
PDF is available here.
PDF is available here.
Abstract
We detail the established functional roles of miRNAs in the embryonic and adult stem cell model systems. Finally, we explore new techniques that exploit endogenous miRNA processing and function for applications in basic and clinical research....
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PMID: 18220910
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)43 2007
Abstract
We demonstrate here that sustained activation of the Wnt pathway (using Wnt3A or an inhibitor of glycogen synthase kinase 3beta) in multiple mouse and human ESCs results in meso/endoderm-specific differentiation. Using monolayer culture conditions, we have generated multipotential "mesendodermal pro...
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PMID: 17711862
PDF is available here.
PDF is available here.
Developmental Dynamics (236)10 2007
Abstract
We describe the expression of Trh during early embryonic development in the mouse. We find Trh to be highly expressed during postimplantation stages in the mouse embryo, with expression first observed in the epiblast at embryonic day (E) 6.5. During gastrulation, Trh is expressed in the newly formed...
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PMID: 17849455
PDF is available here.
PDF is available here.
Developmental Dynamics (236)10 2007
Abstract
We have investigated the effect of ER stress on Xenopus embryogenesis. ER stress induced by tunicamycin (TM) treatment of embryos resulted in defects affecting germ layer formation. We observed up-regulation of ER stress response genes, enhanced cytoplasmic splicing of xXBP1 RNA, and increased rate...
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PMID: 17849439
PDF is available here.
PDF is available here.
Lab On a Chip (7)8 2007
Abstract
We microfabricated adhesive stencils to make mouse ES (mES) cell aggregates of specific sizes ranging from 100 microm to 500 microm in diameter. With this technique, we studied the effect of the initial aggregate size on ES cell differentiation. After 20 days of induction of differentiation, we anal...
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PMID: 17653344
PDF is available here.
PDF is available here.
Differentiation (75)6 2007
Abstract
We review past observations and present new data from studies on the clawed frog Xenopus laevis. Animally localized Sox3 acts to inhibit Nodal (Xnr5 and Xnr6) expression, and induces the expression of genes (Ectodermin, Xema, and Coco) whose products repress Nodal signaling. Vegetally localized Sox7...
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PMID: 17608734
PDF is available here.
PDF is available here.
Biomaterials (28)17 2007
Abstract
We propose a new methodology to enhance the vascular differentiation of human embryonic stem cells (hESCs) by encapsulation in a bioactive hydrogel. hESCs were encapsulated in a dextran-based hydrogel with or without immobilized regulatory factors: a tethered RGD peptide and microencapsulated VEGF(16...
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PMID: 17346788
PDF is available here.
PDF is available here.
Differentiation (75)5 2007
Abstract
We have transplanted EBD cells into the fetal sheep liver. We show that these cells respond appropriately to environmental cues and give rise to hepatocytes and well-structured bile ducts. These results suggest that EBD cells are relatively uncommitted early progenitors capable of effective incorpor...
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PMID: 17286597
PDF is available here.
PDF is available here.
Cryobiology (54)3 2007
Abstract
We evaluated survival adhesion rate, cell structure, gene expression, and multipotency of frozen and thawed embryoid bodies (EBs) derived from mouse ES cells. EBs were cryopreserved using the BICELL and the Program Freezer. After one week the EBs were thawed and cultured. EBs prepared in the Program...
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PMID: 17442298
PDF is available here.
PDF is available here.
Abstract
We developed a novel instrument that uses a pair of parallel wires that are glued to the surface of an embryo normal to the direction in which the stress is to be determined. When a slit is made parallel to the wires and between them, tension in the surrounding tissue causes the slit to open. Under...
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PMID: 17237990
PDF is available here.
PDF is available here.
Stem Cells (25)2 2007
Abstract
We described the derivation of four stable pluripotent rabbit embryonic stem cell (ESC) lines, one (RF) from blastocysts fertilized in vivo and cultured in vitro and three (RP01, RP02, and RP03) from parthenogenetic blastocysts. These ESC lines have been cultivated for extended periods (RF >1 year,...
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PMID: 17038672
PDF is available here.
PDF is available here.
Abstract
Abstract
Here, we show that Xenopus Tsukushi (X-TSK), a member of the secreted small leucine rich repeat proteoglycan (SLRP) family, is expressed in ectoderm, endoderm, and the organizer during early development. We have previously reported that X-TSK binds to and inhibits BMP signaling in cooperation with c...
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PMID: 17925852
PDF is available here.
PDF is available here.
Nucleic Acids Research (35)18 2007
Abstract
We present here two transgenic hESC lines, named ZUN. Each line harbors one copy of the UTF1 promoter/enhancer driving a resistance gene and yielded highly homogeneous cultures under selection pressure, with a larger proportion of Oct4 and Sox2 positive cells. While ZUN cultures, like parental HUES8...
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PMID: 17855398
PDF is available here.
PDF is available here.
Development Genes and Evolution (216)12 2006
Abstract
We used a 4D-microscopy system, which enables us to analyse every cell event in the living embryo. The study was combined with the analysis of the expression of the gene engrailed (en) at different stages of germ band formation. Our findings confirm the results of earlier investigations of the cell...
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PMID: 17006664
PDF is available here.
PDF is available here.
Developmental Dynamics (235)11 2006
Abstract
MicroRNAs (miRNAs) are small, abundant, noncoding RNAs that modulate protein abundance by interfering with target mRNA translation or stability. miRNAs are detected in organisms from all domains and may regulate 30% of transcripts in vertebrates. Understanding miRNA function requires a detailed dete...
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PMID: 17013880
PDF is available here.
PDF is available here.
Abstract
We isolated the full-length cDNA sequence from Lytechinus variegatus and utilized morpholinos to further investigate the role of Endo16 during embryonic development in this species. Endo16-deficient embryos failed to undergo gastrulation and the blastocoele became filled with dissociated cells after...
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PMID: 17026713
PDF is available here.
PDF is available here.
Abstract
The induction of mesoderm and/or endoderm from prospective ectoderm and dorsalization of the marginal zone mesoderm may be linked to inhibition of cell cycling and DNA synthesis in early amphibian embryos. In turn, this may lead to reduction of somatic H1 histone accumulation. A greater number of ce...
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PMID: 17026711
PDF is available here.
PDF is available here.
Abstract
We review organogenesis of the ascidian neural complex comparing embryos and buds in terms of topology, developmental mechanisms and terminology. We propose a new interpretation of bud territories, and reconsider nervous system development based on recent results suggesting that ascidians have verte...
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PMID: 16937358
PDF is available here.
PDF is available here.