Abstract
We investigated whether signalling by TGF-beta3 and Wnt-5a cross-talk during chondrogenic differentiation of chick wing mesenchyme. Using differential display polymerase chain reaction screening, we found the expression of Wnt-5a to be significantly increased during transforming growth factor-beta3...
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PMID: 16183324
PDF is available here.
Abstract
We show that in the receiving cells of the Drosophila wing imaginal disc, lipid-unmodified Hh spreads across many more cell diameters than the wild type and this spreading leads to the activation of low but not high threshold responses. Unlipidated Hh forms become internalized through the apical pla...
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PMID: 16396909
PDF is available here.
Abstract
We provide data in three different epithelia - ventral and dorsal embryonic ectoderm, and larval imaginal disc tissue - showing that cholesterol modification is in fact necessary for the controlled long-range activity of Drosophila Hedgehog. We provide an explanation for the discrepancy between our...
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PMID: 16396912
PDF is available here.
Abstract
Pluripotent embryonic stem (ES) cells have complete potential for all the primary germ layers, such as ectoderm, mesoderm, and endoderm. However, the cellular and molecular mechanisms that control their lineage-restricted differentiation are not understood. Although embryoid bodies, which are formed...
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PMID: 16846024
PDF is available here.
Abstract
We reported that by hanging drops of mouse ES cells, embryoid bodies (EBs) formed gut-like structures in vitro composed of three layers corresponding to the epithelium, lamina propria, and musculature. The morphological features and the process of formation are similar to gut and its organogenesis i...
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PMID: 16846031
PDF is available here.
Abstract
We describe the usefulness and efficacy of this approach using GATA factors as an example....
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PMID: 16246163
PDF is available here.
Abstract
We have analyzed the expression during embryogenesis of the 11 zebrafish Iroquois genes, with special interest for nervous system formation and patterning. During the first 2 days of development, Iroquois genes are expressed in distinct domains in the neuroepithelium, as well as in groups of neurona...
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PMID: 16217788
PDF is available here.
Abstract
We have previously shown that an increase in intracellular Ca2+ is both necessary and sufficient to commit ectoderm to a neural fate in Xenopus embryos. However, the relationship between this Ca2+ increase and the expression of early neural genes has yet to be defined. Using a subtractive cDNA libra...
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PMID: 16214893
PDF is available here.
Abstract
In mammals, left-right symmetry is broken by a mechanically driven leftward flow of liquid at the embryonic node (nodal flow). Various models have emerged explaining how this may happen. Work from Tanaka and colleagues has provided a new mechanism by which nodal flow may be breaking symmetry. They d...
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PMID: 16163731
PDF is available here.
Abstract
We show that dE2F1 and dDP are important for the normal pattern of DNA damage-induced apoptosis in Drosophila wing discs. Unexpectedly, the role that E2F plays varies depending on the position of the cells within the disc. In irradiated wild-type discs, intervein cells show a high level of DNA damag...
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PMID: 16198289
PDF is available here.
Petra P Seemann,
Raphaela R Schwappacher,
Klaus W KW Kjaer,
Deborah D Krakow,
Katarina K Lehmann,
Katherine K Dawson,
Sigmar S Stricker,
Jens J Pohl,
Frank F Plöger,
Eike E Staub,
Joachim J Nickel,
Walter W Sebald,
Petra P Knaus and
Stefan S Mundlos
Abstract
We describe 2 mutations in growth and differentiation factor 5 (GDF5) that alter receptor-binding affinities. They cause brachydactyly type A2 (L441P) and symphalangism (R438L), conditions previously associated with mutations in the GDF5 receptor bone morphogenetic protein receptor type 1b (BMPR1B)...
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PMID: 16127465
PDF is available here.
Abstract
We show that the pharyngeal endoderm induces epibranchial neurogenesis in zebrafish, and that BMP signaling plays a crucial role in this process. Using a her5:egfp transgenic line to follow endodermal movements in living embryos, we show that contact between pharyngeal pouches and the surface ectode...
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PMID: 16077092
PDF is available here.
Abstract
We analyzed the expression profiles of cells induced by ectopic Wingless expression to transdetermine from leg to wing by dissecting transdetermined cells and hybridizing probes generated by linear RNA amplification to DNA microarrays. Changes in expression levels implicated a number of genes: lamin...
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PMID: 16077094
PDF is available here.
Abstract
We demonstrate that endoderm is required for neurogenesis of the zebrafish epibranchial placodes. Mosaic analyses confirm that endoderm is the source of the neurogenic signal. Using a morpholino knockdown approach, we find that fgf3 is required for the majority of placode cells to undergo neurogenes...
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PMID: 16077091
PDF is available here.
Abstract
We have investigated the function of Dlx2 using in ovo electroporation and cell culture. Ectopic expression of Dlx2 within the neural tube beginning prior to emigration of neural crest cells at E1.25 drastically inhibits the migration of transfected cells and induces aggregation of transfected neuro...
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PMID: 15848386
PDF is available here.
Abstract
In both species the branchless, funnel-shaped DV coursed cranially, posteriorly, and slightly oblique to the left side. The DV and the efferent hepatic veins drained into a dilated ampullary area (the collectus venosus) that joined directly with the inferior cava. The length of the DV in baboons inc...
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PMID: 15846194
PDF is available here.
Abstract
There were 9 of 2396 (0.4%) unaffected cases with absent nasal bone (95% confidence interval 0.2%, 0.7%) and 8 of 15 (53.3%) Down syndrome cases (95% confidence interval 26.6%, 78.7%). Using a 1 in 250 risk cut-off, the detection rate of standard first-trimester screening was 87%, with a false-posit...
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PMID: 15846188
PDF is available here.
Abstract
Telomere length negatively predicted fragmentation in day 3 preimplantation embryos, after controlling for patient age and basal follicle stimulating hormone level. Telomere length did not predict other features of preimplantation embryo morphology. CONCLUSION: The finding that telomere length in hu...
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PMID: 15846215
PDF is available here.
Abstract
Lens arises from ectoderm situated next to the optic vesicles. By thickening and invaginating, the ectoderm forms the lens vesicle. Growth factors are key regulators of cell fate and behavior. Current evidence indicates that FGFs and BMPs are required to induce lens differentiation from ectoderm. In...
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PMID: 15766743
PDF is available here.
Abstract
We used vital dyes to label exposed anterior endoderm cells of early somite stage mouse embryos, cultured the embryos into the tissue bud phase of development, and determined the tissue fate of the dye labeled cells. This approach was performed at three embryonic stages that are prior to, or coincid...
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PMID: 15766750
PDF is available here.
Abstract
We investigated the expressions of Delta like (Dll) 1 and Dll4 in the mouse thymus anlages. In the normal thymus anlages on embryonic day 13, Dll4 is strongly expressed throughout the epithelial region, but Dll1 is expressed only in scattered cells. In contrast, epithelial cells of the nude thymus a...
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PMID: 16095582
PDF is available here.
Abstract
We have examined wound healing during regeneration of Drosophila wing imaginal discs fragments by confocal microscopy and assessed the role of components of the JNK pathway in this process. After cutting, columnar and peripodial epithelia cells at the wound edge start to close the wound through form...
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PMID: 15766749
PDF is available here.
Abstract
We found that both testosterone and estradiol promoted proliferation of cells in both normal DRG and the Froriep's ganglia. By contrast, estradiol significantly increased the number of apoptotic cells, while testosterone strongly inhibited apoptosis. These actions of sex steroids on DRG development...
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PMID: 15763271
PDF is available here.
Abstract
We found that neurogenesis in Pax6 mutant optic vesicles was not arrested, but instead accelerated as numerous neurons differentiated precociously, more than a day earlier than normal. To identify potential mechanisms for Pax6 repression of neuron differentiation, we examined retinal proliferation a...
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PMID: 15733660
PDF is available here.