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Enzymes and Coenzymes (4)
Articles on Enzymes and Coenzymes
Eukaryotic pyruvate formate lyase and its activating enzyme were acquired laterally from a Firmicute.
We have comprehensively searched for homologs of Pfl and Pfla in publicly available large-scale eukaryotic genomic and cDNA sequencing data, including our own from the anaerobic amoebozoan Mastigamoeba balamuthi. Surprisingly, we find that these enzymes are widely distributed and are present in dive...
Effect of BTH on anthocyanin content and activities of related enzymes in Strawberry after harvest.
These results indicated that the increase in anthocyanin content by BTH might result from the activation of its related enzymes. These data are the first evidence that BTH induces enzyme activities related to anthocyanin metabolism in strawberry fruit after harvest....
Mass spectrometric method incorporating enzymatic amplification for attomole-level analysis of target metabolites in biological samples.
We report a sensitive method for selective detection of target metabolites from the central metabolic pathway by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), in which the MS signal is enhanced by up to three orders of magnitude in the course of in situ enzymatic amplif...
Regulation of cellular metabolism by protein lysine acetylation.
We show that lysine acetylation is a prevalent modification in enzymes that catalyze intermediate metabolism. Virtually every enzyme in glycolysis, gluconeogenesis, the tricarboxylic acid (TCA) cycle, the urea cycle, fatty acid metabolism, and glycogen metabolism was found to be acetylated in human...
Cell biology. Rise of the rival.
Spontaneous high-yield production of hydrogen from cellulosic materials and water catalyzed by enzyme cocktails.
Correction of pre-steady-state KIEs for isotopic impurities and the consequences of kinetic isotope fractionation.
We show, both experimentally and by kinetic modeling, that enzymatic single-turnover (pre-steady-state) H-transfer reactions can be significantly complicated by kinetic isotope fractionation. This fractionation results in the formation of more protiated than deuterated product and is a unique proble...
Augmented antioxidant status in Tamoxifen treated postmenopausal women with breast cancer on co-administration with Coenzyme Q10, Niacin and...
Enhanced oxidative stress as evidenced by increased lipids and lipid peroxides with decreased AO levels in untreated breast cancer patients was observed. Adjuvant TAM-treated group had a limited impact on the increased oxidative stress with decreased AO status. Severe hypertriglycerdemia was observe...
Structure-based drug design targeting biosynthesis of isoprenoids: a crystallographic state of the art of the involved enzymes.
Biosynthesis of the universal terpenoid precursors, isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), from three acetyl CoA moieties through mevalonate was studied extensively in the 1950s. For several decades, the mevalonate paradigm reigned supreme and a mevalonate origin was at...
Acetamido sugar biosynthesis in the Euryarchaea.
Archaea and eukaryotes share a dolichol phosphate-dependent system for protein N-glycosylation. In both domains, the acetamido sugar N-acetylglucosamine (GlcNAc) forms part of the core oligosaccharide. However, the archaeal Methanococcales produce GlcNAc using the bacterial biosynthetic pathway. Key...