Find your PDFs fast.
Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
DNA Restriction Enzymes (3):
Recent article
DNA Restriction Enzymes
Voprosy Virusologii (56)1 2011
Abstract
The paper describes a simple, rapid screening of samples potentially containing Crimean-Congo hemorrhagic fever (CCHF) virus strains, by applying the restriction analysis of amplicones, for the differentiation of CCHF virus genotypes that are characteristic of Europe from virus biovariants uncharacte...
|
PMID: 21427952
PDF is available here.
PDF is available here.
| < More recent | Older article > |
Amplification of unknown RNAs and RNA mixtures based on unique restriction enzyme cleavage in vitro.
Abstract
We have developed a novel method to amplify target RNA. The amplification procedure was carried out by sequential RT-PCR, effective separation, restriction enzymatic cleavage of cDNA strand, and run-off transcription in vitro of target RNA from its cDNA. Introduction of a unique stem-loop linker int...
|
PMID: 21106769
PDF is available here.
PDF is available here.
Abstract
We present here a PCR-based method that allows a priori determination of the degree of chloroplast and mitochondrial contamination in DNA samples from plant environments. It is based on differential digestibility of chloroplast, mitochondrial and bacterial small subunit rRNA gene amplicons with the...
|
PMID: 20816905
PDF is available here.
PDF is available here.
Abstract
The persistence of covalently closed circular DNA (cccDNA) of hepatitis B virus (HBV) in hepatocytes plays a key role in viral persistence and resistance to therapy. Therefore, quantitative cccDNA measurement is of clinical importance for evaluating the efficacy of antiviral drugs, selecting an appr...
|
PMID: 20691734
PDF is available here.
PDF is available here.
Clinical Chemistry (56)7 2010
Abstract
Our novel DNA methylation assay reduces both the hands-on time and errors caused by handling and pipetting and allows methylation analyses to be completed within 90 min after DNA extraction. Combined with its precision and reliability, these features make the assay well suited for diagnostic procedu...
|
PMID: 20472822
PDF is available here.
PDF is available here.
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Clinical Chemistry (56)7 2010
Abstract
Abstract
We perform transposon insertion profiling by microarray (TIP-chip) to map human L1(Ta) retrotransposons (LINE-1 s) genome-wide. This identified numerous novel human L1(Ta) insertional polymorphisms with highly variant allelic frequencies. We also explored TIP-chip's usefulness to identify candidate...
|
PMID: 20602999
PDF is available here.
PDF is available here.
Aging Cell (9)3 2010
Abstract
We have a tool that can directly investigate the relationship between senescence and the load of short telomeres. The method is a variant of the chromosome-specific STELA method but has the advantage that it can demonstrate short telomeres regardless of chromosome. With Universal STELA, we find a st...
|
PMID: 20331440
PDF is available here.
PDF is available here.
Aging Cell (9)3 2010
Abstract
We have a tool that can directly investigate the relationship between senescence and the load of short telomeres. The method is a variant of the chromosome-specific STELA method but has the advantage that it can demonstrate short telomeres regardless of chromosome. With Universal STELA, we find a st...
|
PMID: 20331440
PDF is available here.
PDF is available here.
Aging Cell (9)3 2010
Abstract
We have a tool that can directly investigate the relationship between senescence and the load of short telomeres. The method is a variant of the chromosome-specific STELA method but has the advantage that it can demonstrate short telomeres regardless of chromosome. With Universal STELA, we find a st...
|
PMID: 20331440
PDF is available here.
PDF is available here.
Aging Cell (9)3 2010
Abstract
We have a tool that can directly investigate the relationship between senescence and the load of short telomeres. The method is a variant of the chromosome-specific STELA method but has the advantage that it can demonstrate short telomeres regardless of chromosome. With Universal STELA, we find a st...
|
PMID: 20331440
PDF is available here.
PDF is available here.
Aging Cell (9)3 2010
Abstract
We have a tool that can directly investigate the relationship between senescence and the load of short telomeres. The method is a variant of the chromosome-specific STELA method but has the advantage that it can demonstrate short telomeres regardless of chromosome. With Universal STELA, we find a st...
|
PMID: 20331440
PDF is available here.
PDF is available here.
Abstract
We describe the potential of microchip electrophoresis with a Hitachi SV1100, which can be used to determine DNA sizes between 500 and 5000 bp with good quantification (DNA concentration, <8 ng/l) within 5 min, for the analysis of DNA ligation. On analysis of an electropherogram of a ligation mixtur...
|
PMID: 20196235
PDF is available here.
PDF is available here.
Abstract
1) The positive relationship previously found between the percentage of homoplasy and genome size is a direct consequence of the number of observed bands and the GC content. For the same number of observed bands, the percentage of homoplasy is independent of the genome size of the species. 2) The di...
|
PMID: 20056690
PDF is available here.
PDF is available here.
Abstract
This paper reports a new strategy, recursive directional ligation by plasmid reconstruction (PRe-RDL), to rapidly clone highly repetitive polypeptides of any sequence and specified length over a large range of molecular weights. In a single cycle of PRe-RDL, two halves of a parent plasmid, each cont...
|
PMID: 20184309
PDF is available here.
PDF is available here.
Abstract
I enzymes that cut DNA randomly, but in terms of gene organization and protein assembly, most Type IIB restriction-modification systems have more in common with Type I than with other Type II systems. Our current knowledge of the Type IIB systems is reviewed in the present paper....
|
PMID: 20298193
PDF is available here.
PDF is available here.
Methods in Molecular Biology (631)631 2010
Abstract
We have shown that the combined bisulfite restriction analysis (COBRA) assay makes it possible to analyze methylation levels at a defined locus. The major steps are: bisulfite conversion of nonmethylate cytosines to uracils, locus-specific PCR amplification of converted DNA, restriction digestion, a...
|
PMID: 20204865
PDF is available here.
PDF is available here.
Methods in Molecular Biology (607)607 2010
Abstract
We have shown that the wheat-germ-based cell-free protein synthesis is useful for X-ray crystallography of one of the 4-bp cutter restriction enzymes, which are expected to be very toxic to all forms of cells retaining the genome. Our report on its structure represents the first report of structure...
|
PMID: 20204855
PDF is available here.
PDF is available here.
Genome Research (20)2 2010
Abstract
We have developed a novel approach for using massively parallel short-read sequencing to generate fast and inexpensive de novo genomic assemblies comparable to those generated by capillary-based methods. The ultrashort (<100 base) sequences generated by this technology pose specific biological and c...
|
PMID: 20123915
PDF is available here.
PDF is available here.
Abstract
We have explored the possibility of modulating the activity of a restriction enzyme with light. By cross-linking two suitably located cysteine residues with a bifunctional azobenzene derivative, which can adopt a cis- or trans-configuration when illuminated by UV or blue light, respectively, enzymat...
|
PMID: 20080559
PDF is available here.
PDF is available here.
Abstract
Abstract
Abstract
We will learn much from the study of epigentics --factors that can affect the phenotype of an organism without changing the genetic information--. He is proud that, in that studying restriction degradation and DNA methylation in the 1960s, he was among the first in studying epigenetic phenomenon....
|
PMID: 20220745
PDF is available here.
PDF is available here.
Abstract
Stable integration of cloned gene products into the Xenopus genome is necessary to control the time and place of expression, to express genes at later stages of embryonic development, and to define how enhancers and promoters regulate gene expression within the embryo. The protocol demonstrated here...
|
PMID: 20811326
PDF is available here.
PDF is available here.
Abstract
Screening of plasmid DNA was carried out among 94 streptomycetes cultures which were isolated from the samples of Ukrainian soils with different anthropogenic contamination. Seventeen streptomycetes strains containing plasmid DNA were found. It is established that some cultures contain more than one...
|
PMID: 21117293
PDF is available here.
PDF is available here.