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Apyrase (0):
Recent article
Apyrase
Stem Cell Research (7)1 2011
Abstract
We investigated the involvement of ADO in the immunomodulation promoted by MSCs. Human T lymphocytes were activated and cultured with or without MSCs. Compared to lymphocytes cultured without MSCs, co-cultured lymphocytes were suppressed and expressed higher levels of ADORA2A and lower levels of ADA...
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PMID: 21546330
PDF is available here.
PDF is available here.
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Abstract
Schistosoma mansoni ATP diphosphohydrolase isoforms and potato apyrase share conserved epitopes. By enzyme-linked immunosorbent assays, elevated levels of IgM, IgG2a and IgG1 antibody reactivity against potato apyrase were observed in S. mansoni-infected BALB/c mice during the acute phase of infecti...
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PMID: 20721478
PDF is available here.
PDF is available here.
Abstract
We showed for the first time that the conserved domains within Schistosoma mansoni ATP diphosphohydrolase isoforms, shared with potato apyrase, possess epitopes for the IgG1 and IgG4 subtypes, as 24 (80%) of the 30 schistosomiasis patients were seropositive for this vegetable protein. The analyses f...
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PMID: 20721477
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (26)6 2010
Abstract
The expression of CTLA-4, HLA-DR, CD45RO and Ki-67 was significantly higher in CD39(+);FoxP3(+); Treg cells than those in CD39(-);FoxP3(+); Treg cells. The suppressive capacity of CD39(+);FoxP3(+); Treg cells on proliferation and IFN-gamma secretion of Treg-depleted PBMC was significantly higher tha...
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PMID: 20487644
PDF is available here.
PDF is available here.
Abstract
We tested this relationship in vitro and ex vivo by measuring upregulation of B-cell surface molecules, the percentage of cells activated, and cytokine secreted in response to lipopolysaccharide (LPS) activation. In vitro, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) improved the membr...
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PMID: 20071694
PDF is available here.
PDF is available here.
Transplantation Reviews (24)2 2010
Abstract
Despite tremendous improvements in short-term renal allograft survival, many patients still have chronic rejection or side effects of nonspecific immunosuppression. The discovery of Foxp3(+) regulatory T cells (Tregs) has revolutionized the concepts in immunoregulation and offers perspectives for ov...
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PMID: 20153159
PDF is available here.
PDF is available here.
Abstract
These results identify a glucose-sensitive transcription pathway in vivo, revealing a novel molecular mechanism that may underlie vascular complications of diabetes....
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PMID: 19965778
PDF is available here.
PDF is available here.
Thrombosis and Haemostasis (103)2 2010
Abstract
We investigated whether circulating human solCD39 could reduce platelet activation and accumulation, thus abating leukocyte infiltration and neointimal formation following vascular damage. Intraperitoneally-administered solCD39 ADPase activity in plasma peaked 1 hour (h) post-injection, with an elim...
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PMID: 20024507
PDF is available here.
PDF is available here.
Thrombosis and Haemostasis (103)2 2010
Abstract
Ukrainskij biokhimicheskij zhurnal/Ukrayins'kij biokhimichnij zhurnal/Ukrainian biochemical journal (82)2 2010
Abstract
Influence of DL-homocysteine thiolactone loading (100 mg/kg by intragastric administration for 28 days) on enzymes activity of adenylic nucleotide and adenosine metabolism in the blood serum, platelets and liver of rats was investigated. The relation between revealed disturbance and platelet hyper-r...
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PMID: 20684246
PDF is available here.
PDF is available here.
Journal of Immunology (183)10 2009
Abstract
We identify pericellular adenosine, interacting with T cell-associated G protein-coupled A(2A/B) adenosine receptors (AR), as contributing to FL T cell hyporesponsiveness. In a subset of FL patient samples, treatment of lymph node mononuclear cells (LNMC) with specific A(2A/B) AR antagonists results...
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PMID: 19864600
PDF is available here.
PDF is available here.
Methods in Molecular Biology (576)576 2009
Abstract
We chose qRT-PCR analysis of CD39 in pancreatic tissue as an example that is applicable to any gene of interest. CD39/ecto-nucleoside triphosphate diphosphohydrolase-type-1 (ENTPD1) is the dominant vascular ecto-nucleotidase that hydrolyzes extracellular nucleotides to integrate purinergic signaling...
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PMID: 19882271
PDF is available here.
PDF is available here.
Transplantation (88)3 2009
Abstract
We show that the frequency of CD4/CD25/FoxP3 Treg in the peripheral blood increases after each cycle of ECP and also in the course of treatment. The suppressive capacity of Treg after ECP was increased compared with that of Treg before ECP, although not reaching the suppression levels obtained with...
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PMID: 19667946
PDF is available here.
PDF is available here.
Journal of Immunological Methods (346)1-2 2009
Abstract
Human regulatory T cells (Treg) have been variously defined as CD4(+)CD25(+), CD4(+)CD25(high) or CD4(+)CD25(high)FOXP3(+) cells which are responsible for maintaining peripheral tolerance. Their isolation from human peripheral blood or tissues depends on the expression level of CD25(IL-2Ralpha) - a...
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PMID: 19450601
PDF is available here.
PDF is available here.
Abstract
We noted that basal portal pressures (PPs) were comparable in wild-type and Cd39-null mice (n = 9). ATP infusions resulted in increments in PP in wild-type mice, but, in contrast, this significantly decreased in Cd39-null mice (n = 9) post-ATP in a nitric oxide-dependent manner. We then studied Cd39...
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PMID: 19520738
PDF is available here.
PDF is available here.
Hearing Research (253)1-2 2009
Abstract
Signalling actions of extracellular nucleotides via P2 receptors influence cellular function in most tissues. In the inner ear, P2 receptor signaling is involved in many processes including the regulation of hearing sensitivity and the cochlea's response to noise stress. CD39 (NTPDas...
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PMID: 19327391
PDF is available here.
PDF is available here.
Hearing Research (253)1-2 2009
Abstract
Signalling actions of extracellular nucleotides via P2 receptors influence cellular function in most tissues. In the inner ear, P2 receptor signaling is involved in many processes including the regulation of hearing sensitivity and the cochlea's response to noise stress. CD39 (NTPDas...
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PMID: 19327391
PDF is available here.
PDF is available here.
Hearing Research (253)1-2 2009
Abstract
Signalling actions of extracellular nucleotides via P2 receptors influence cellular function in most tissues. In the inner ear, P2 receptor signaling is involved in many processes including the regulation of hearing sensitivity and the cochlea's response to noise stress. CD39 (NTPDas...
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PMID: 19327391
PDF is available here.
PDF is available here.
Hearing Research (253)1-2 2009
Abstract
Signalling actions of extracellular nucleotides via P2 receptors influence cellular function in most tissues. In the inner ear, P2 receptor signaling is involved in many processes including the regulation of hearing sensitivity and the cochlea's response to noise stress. CD39 (NTPDas...
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PMID: 19327391
PDF is available here.
PDF is available here.
Hearing Research (253)1-2 2009
Abstract
Signalling actions of extracellular nucleotides via P2 receptors influence cellular function in most tissues. In the inner ear, P2 receptor signaling is involved in many processes including the regulation of hearing sensitivity and the cochlea's response to noise stress. CD39 (NTPDas...
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PMID: 19327391
PDF is available here.
PDF is available here.
Hearing Research (253)1-2 2009
Abstract
Signalling actions of extracellular nucleotides via P2 receptors influence cellular function in most tissues. In the inner ear, P2 receptor signaling is involved in many processes including the regulation of hearing sensitivity and the cochlea's response to noise stress. CD39 (NTPDas...
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PMID: 19327391
PDF is available here.
PDF is available here.
Hearing Research (253)1-2 2009
Abstract
Signalling actions of extracellular nucleotides via P2 receptors influence cellular function in most tissues. In the inner ear, P2 receptor signaling is involved in many processes including the regulation of hearing sensitivity and the cochlea's response to noise stress. CD39 (NTPDas...
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PMID: 19327391
PDF is available here.
PDF is available here.
Hearing Research (253)1-2 2009
Abstract
Signalling actions of extracellular nucleotides via P2 receptors influence cellular function in most tissues. In the inner ear, P2 receptor signaling is involved in many processes including the regulation of hearing sensitivity and the cochlea's response to noise stress. CD39 (NTPDas...
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PMID: 19327391
PDF is available here.
PDF is available here.
Abstract
Extracellular nucleotides might influence aspects of the biology of reproduction in that ATP affects smooth muscle contraction, participates in steroidogenesis and spermatogenesis, and also regulates transepithelial transport, as in oviducts. Activation of cellular nucleotide purinergic receptors is...
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PMID: 19125273
PDF is available here.
PDF is available here.
Abstract
We show that astrocytes in culture exhibit basal P2X7R activity. Primary mouse astrocytes were found to take up the P2X7R permeant dyes YO-PRO-1 (YP) and propidium iodide in absence of any added ligands. By contrast, cultured rat astrocytes took up very little YP, consistent with their much lower le...
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PMID: 18942742
PDF is available here.
PDF is available here.
Abstract
We show a detailed immunocytochemical localization of two enzymes of the E-NTPDase family in the retinal layers of two vertebrate species, namely, the mouse and the zebrafish. In the mouse retina, NTPDase2 was chiefly localized in Müller glia and ganglion cell processes. NTPDase1 was located on n...
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PMID: 19116950
PDF is available here.
PDF is available here.
Electrophoresis (30)6 2009
Abstract
We successfully prepared and used beaded apyrase, which can be removed from the detection mixture before sample typing, to decrease the high background due to adenosine 5'-triphosphate and inorganic pyrophosphate or sodium pyrophosphate decahydrate contamination. Unlike gel-based pyrosequencing, chi...
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PMID: 19235806
PDF is available here.
PDF is available here.
Abstract
CD4+ T cells expressed CD39 and CD73, particularly in CD25+Foxp3+ Treg from peripheral blood or gastric mucosa. Activation significantly increased CD73 expression on all Th cells. Inhibition of CD73 enhanced production of interferon-gamma. Gastritis in H. felis-infected CD73-/- mice was significantl...
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PMID: 19281303
PDF is available here.
PDF is available here.
Abstract
ATP was primarily metabolized via ecto-ATPDase (adenosine 5'-triphosphate diphosphohydrolase) into AMP, which was then dephosphorylated into adenosine by ecto-5'-nucleotidase. Alternative conversion of ATP into ADP by ecto-ATPase (adenosine 5'-triphosphatase) was more relevant at high ATP concentrat...
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PMID: 19154428
PDF is available here.
PDF is available here.
Abstract
Xenopus follicle-enclosed oocytes are endowed with purinergic receptors located in the follicular cell membrane; their stimulation by ATP elicits an electrical response that includes generation of a fast inward current (F(Cl)) carried by Cl(-). Here, it was found that mechanical stimulation of the f...
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PMID: 18932209
PDF is available here.
PDF is available here.
Transplantation (86)12 2008
Abstract
Compared with wild-type mice, Cd73 null mice showed reduced levels of serum creatinine and urea, apoptosis of renal cells, and histologic damage after I/RI. Deletion of CD39 was associated with severe renal injury. Administration of apyrase, a soluble form of CD39, decreased global apoptosis and I/R...
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PMID: 19104409
PDF is available here.
PDF is available here.
Gastroenterology (135)5 2008
Abstract
Regulated phosphohydrolysis of extracellular nucleotides by CD39 coordinates both hepatocyte and endothelial cell proliferation following partial hepatectomy. Lack of CD39 activity is associated with decreased hepatic regeneration and failure of vascular reconstitution....
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PMID: 18804472
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)42 2008
Abstract
We show that that ecto-nucleoside triphosphate diphosphohydrolase 1 (E-NTPDase1, CD39) is a critical enzyme for hydrolysis of released ATP by neutrophils and for cell migration in response to multiple agonists (N-formyl-methionyl-leucyl-phenylalanine, interleukin-8, and C5a). Upon stimulation of hum...
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PMID: 18713747
PDF is available here.
PDF is available here.
Plant and Cell Physiology (49)10 2008
Abstract
We used intact potato (Solanum tuberosum) tuber slices as an experimental system enabling access to the apoplast without interference of cytosolic contamination. (i) Incubation of intact tuber slices with ATP led to the formation of ADP, AMP, adenosine, adenine and ribose, indicating operation of ap...
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PMID: 18772187
PDF is available here.
PDF is available here.
Abstract
We determined whether the effect of triamcinolone is mediated by a receptor-dependent mechanism. Hyperglycemia was induced in rats with streptozotocin injection. After 6-10 months, the swelling properties of glial cells in retinal slices upon hypotonic challenge were determined. Nucleotide-degrading...
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PMID: 18687327
PDF is available here.
PDF is available here.
Biophysical Journal (95)7 2008
Abstract
We have used negative staining electron microscopy to study the effects of blebbistatin on the organization of the myosin heads on muscle thick filaments. Loss of ADP and Pi from the heads causes thick filaments to lose their helical ordering. In the presence of 100 microM blebbistatin, disordering...
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PMID: 18599626
PDF is available here.
PDF is available here.
Abstract
CD39/Entpd1 is a modulator of extracellular nucleotide signaling and also influences metabolism. Deletion of Cd39/Entpd1 both directly and indirectly impacts insulin regulation and hepatic glucose metabolism. Extracellular nucleotides serve as "metabolokines," indicating further links between inflam...
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PMID: 18567823
PDF is available here.
PDF is available here.
Hepatology (48)3 2008
Abstract
We show that NKT cells express both CD39 and CD73/ecto-5'-nucleotidase and can therefore generate adenosine from extracellular nucleotides, whereas natural killer cells do not express CD73. In vivo, mice null for CD39 are protected from Con A-induced liver injury and show substantively lower serum l...
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PMID: 18752325
PDF is available here.
PDF is available here.
Abstract
NTPDase and 5'-nucleotidase activities increased with increasing glucose and fructose concentrations (P<0.001) and the different times of pre-incubation did not interfere in ectonucleotidases activities (P>0.5). NTPDase and 5'-nucleotidase activities demonstrated a positive correlation between serum...
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PMID: 18644642
PDF is available here.
PDF is available here.
Abstract
We found that P. gingivalis provoked aggregation, whereas epinephrine alone never had any effect. Intriguingly, pre-treatment of platelets with a low, sub-threshold number of P. gingivalis (i.e. a density that did not directly provoke platelet aggregation) resulted in a marked aggregation response w...
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PMID: 18791941
PDF is available here.
PDF is available here.
Abstract
The presented data suggest that increasing CD39/NTPDase-1 activity in VSMC could represent a novel therapeutic approach for the prevention of stenosis associated with angioplasty and other occlusive vascular diseases....
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PMID: 18485080
PDF is available here.
PDF is available here.
Parasitology (135)8 2008
Abstract
Evolutionary and closer structural relationships are demonstrated by phylogenetic analysis, peptide prediction and molecular modelling between Solanum tuberosum apyrase, Schistosoma mansoni SmATPase 2 and Leishmania braziliensis NDPase. Specific protein domains are suggested to be potentially involv...
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PMID: 18598576
PDF is available here.
PDF is available here.
Plant Physiology (147)3 2008
Abstract
We used reverse genetic and biochemical approaches to investigate the role of potato (Solanum tuberosum)-specific apyrase. Silencing of the apyrase gene family with RNA interference constructs under the control of the constitutive 35S promoter led to a strong decrease in apyrase activity to below 10...
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PMID: 18480378
PDF is available here.
PDF is available here.
Abstract
We examined the role of a novel soluble apyrase/ADPase, APT102, and an inhibitor of TXA(2) synthesis, acetylsalicylic acid (aspirin or ASA), in mouse models of experimental bone metastases. We found that treatment with ASA and APT102 in combination (ASA + APT102), but not either drug alone, signific...
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PMID: 18260128
PDF is available here.
PDF is available here.
Abstract
We reported that activation of G protein-coupled receptors (GPCR) in 1321N1 human astrocytoma cells elicits a rapid release of ATP that is partially dependent on a G(q)/phophospholipase C (PLC)/Ca(2+) mobilization signaling cascade. In this study we assessed the role of Rho-family GTPase signaling a...
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PMID: 18495810
PDF is available here.
PDF is available here.
Experimental Parasitology (119)2 2008
Abstract
Nucleotidase cascades (apyrase, 5' nucleotidase, and adenosine deaminase (ADA) were investigated in the parasitic trematode Fasciola gigantica. ADA had the highest activity in the nucleotidase cascades. Adenosine deaminase was purified from F. gigantica through acetone precipitation and chromatograp...
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PMID: 18436213
PDF is available here.
PDF is available here.
Neurochemical Research (33)6 2008
Abstract
Our results suggest that alterations caused by GAA in the activities of these enzymes may contribute to the understanding of the neurological dysfunction of GAMT-deficient patients....
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PMID: 18256932
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)19 2008
Abstract
We identified an ecto-nucleoside triphosphate diphosphohydrolase (NTPDase) from L. pneumophila, termed Lpg1905, which enhances intracellular replication of L. pneumophila in eukaryotic cells. Lpg1905 is the first prokaryotic member of the CD39/NTPDase1 family of enzymes, which are characterized by t...
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PMID: 18337253
PDF is available here.
PDF is available here.
Abstract
Embryonic human retina has a pool of precursors (CXCR4(+) and c-Kit(+)) that enlarged centrifugally during fetal development. From this pool emerges angioblasts, which migrate anteriorly into the nerve fiber layer where SDF-1 and SCF levels are highest. c-Kit expression declines with apparent migrat...
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PMID: 18436851
PDF is available here.
PDF is available here.
Abstract
We hypothesized that transfected Giardia might be used to identify the UDP-Glc transporter of Entamoeba. Here we show Giardia membranes transport UDP-GlcNAc and have apyrases, which hydrolyze nucleoside-diphosphates to make the antiporter nucleoside-monophosphate. The only NST of Giardia (GlNst), wh...
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PMID: 18346800
PDF is available here.
PDF is available here.
Abstract
Our results indicate that the strengths of interaction of the respective TMD pairs of the human NTPDase2 and chicken NTPDase8 determine their different responses to membrane perturbation and substrate....
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PMID: 18295590
PDF is available here.
PDF is available here.
Abstract
Our results suggest that ATP release is an early event and that eATP plays a signaling role in the elicitation of plant cell responses; Ca2+ is required for activation of the elicitor-induced ATP release and the eATP signal transduction. This is the first report on ATP release induced by a fungal el...
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PMID: 18325935
PDF is available here.
PDF is available here.
Abstract
Studies in mice with null mutations of adenosine 1 receptor or ecto-5'-nucleotidase genes suggest a critical role of adenosine and its precursor 5'-AMP in tubulovascular signaling. To assess whether the source of juxtaglomerular nucleotides can be traced back to ATP dephosphorylation, experiments we...
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PMID: 18256308
PDF is available here.
PDF is available here.
Parasitology (135)3 2008
Abstract
A Leishmania (Leishmania) amazonensis ATP diphosphohydrolase isoform was partially purified from plasma membrane of promastigotes by preparative non-denaturing polyacrylamide gel electrophoresis. SDS-PAGE followed by Western blots developed with polyclonal anti-potato apyrase antibodies identified d...
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PMID: 18005473
PDF is available here.
PDF is available here.
Abstract
We show that inhibition of adenosine receptors impedes ATP triggered migration. CD39 is the dominant cellular ectonucleotidase that degrades nucleotides to nucleosides, including adenosine. Importantly, ATP fails to stimulate P2 receptor mediated migration in cd39(-/-) microglia. However, the effect...
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PMID: 18098126
PDF is available here.
PDF is available here.
Abstract
We observed previously that the extent of ADP-induced platelet aggregation in blood from patients with leucocytosis is markedly reduced. We obtained evidence that this is via enhanced ADP metabolism consequent to the high leucocyte count, and speculated that ecto-NTPDase CD39 on leucocytes may be in...
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PMID: 18231939
PDF is available here.
PDF is available here.
Abstract
We present the analysis of a set of 1534 (SG) cDNA sequences, 645 of which coded for proteins of a putative secretory nature. Most salivary proteins described as lipocalins matched peptide sequences obtained from proteomic results....
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PMID: 18207082
PDF is available here.
PDF is available here.
Abstract
Abstract
We demonstrated that the mutation of glutamic acid 130 (located in the dimer interface) to tyrosine increased both the tendency to form dimers and the ADPase activity. In the present study, we investigated the importance of the dimeric state for enzymatic activity and biological function in this nuc...
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PMID: 18067325
PDF is available here.
PDF is available here.
Acta Biochimica Polonica (55)1 2008
Abstract
Nucleoside triphosphate diphosphohydrolase--NTPDase1 (apyrase, EC 3.6.1.5) was modeled based on sequence homology. The single polypeptide chain of apyrase is folded into two domains. The putative catalytic site with the apyrase conserved regions (ACR 1-5) is located between these two domains. Modeli...
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PMID: 18335113
PDF is available here.
PDF is available here.