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Nucleoside-Triphosphatase (0):
Recent article
Nucleoside-Triphosphatase
Journal of Biological Chemistry (285)30 2010
Abstract
We found that the 5' termini of the transcripts are diphosphorylated. The 5'-terminal nucleotide is G, and GDP was the best substrate among those examined to prime the reaction. When GTP was used, the triphosphate of GTP incorporated into the 5'-end was converted to diphosphate. This activity was no...
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PMID: 20511225
PDF is available here.
PDF is available here.
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Abstract
We concluded that the mAbs against NTPase-II can reduce the replication of T. gondii and have a crucial effect on the protection of host from T. gondii infection....
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PMID: 20056166
PDF is available here.
PDF is available here.
Malaria Journal (8)1 2009
Abstract
Based upon distinct sequence features and secondary structure profile of the P-loop domain of obtained sequences, a cladistic classification is also conceded: nucleotide kinases and GTPases, ABC and SMC family, SF1/2 helicases, AAA+ and AAA protein families. Attempts are made to identify any ortholo...
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PMID: 19374766
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)45 2008
Abstract
We recently identified a TTM protein from the bacterium Clostridium thermocellum (CthTTM) with the opposite substrate preference. Here we report that CthTTM catalyzes hydrolysis of guanosine 5'-tetraphosphate to yield GTP and P(i) (K(m) = 70 microm, k(cat) = 170 s(-1)) much more effectively than it...
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PMID: 18782773
PDF is available here.
PDF is available here.
Abstract
We have identified that the NTP binding site occurs close to Trp106 and the area around the nucleotide binding site is predominantly negatively charged. UsfX binds to a variety of nucleotides unlike other reported anti-sigma factors and exhibits an unusual dual NTPase activity. In silico computation...
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PMID: 18722345
PDF is available here.
PDF is available here.
Abstract
NTPDase and 5'-nucleotidase activities increased with increasing glucose and fructose concentrations (P<0.001) and the different times of pre-incubation did not interfere in ectonucleotidases activities (P>0.5). NTPDase and 5'-nucleotidase activities demonstrated a positive correlation between serum...
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PMID: 18644642
PDF is available here.
PDF is available here.
Abstract
We synthesized adenine and uracil nucleotide mimetics, in which the phosphate residues were replaced by phosphonic acid esters attached to the nucleoside at the 5'-position by amide linkers. Among the synthesized uridine derivatives, we identified the first potent and selective inhibitors of human N...
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PMID: 18630897
PDF is available here.
PDF is available here.
Virus Research (135)1 2008
Abstract
We were unable to detect helicase activity at protein concentrations up to 500nM. It has been reported that longer constructions of NS3 helicase domains from other flavivirus, like those which include residues of the linker region between the protease and the helicase domains, have helicase activity...
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PMID: 18448185
PDF is available here.
PDF is available here.
Abstract
I and either ATP or dATP. This argues for a two step model: the specifying step, requiring both VTF and UUUUUNU, and the energy-dependent step employing NPH I and ATP. Evaluation of NPH I mutants for the ability to stimulate transcription elongation demonstrated that ATPase activity and a stable int...
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PMID: 18433825
PDF is available here.
PDF is available here.
Molecular Microbiology (68)6 2008
Abstract
We show that genomic regions flanking 16 parasite genes, which encompass examples of constitutive and tachyzoite- and bradyzoite-specific genes, are able to reproduce the appropriate developmental stage expression in a transient luciferase assay. Mapping of cis-acting elements in several bradyzoite...
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PMID: 18433450
PDF is available here.
PDF is available here.
Biochemistry (New York) (73)6 2008
Abstract
A method has been developed for obtaining a full-length protein NS3 of hepatitis C virus with the yield of 6.5 mg/liter of cell culture, and conditions for measuring its NTPase and helicase activities have been optimized. The helicase reaction can proceed in two modes depending on the enzyme and sub...
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PMID: 18620531
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)22 2008
Abstract
We describe the first crystal structure of active MazG from E. coli, which is composed of two similarly folded globular domains in tandem. Among the two putative catalytic domains, only the C-terminal domain has well ordered active sites and exhibits an NTPase activity. The MazG-ATP complex structur...
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PMID: 18353782
PDF is available here.
PDF is available here.
Abstract
These results suggest that the pool of selected aptamers might be potentially useful as anti-SCV agents....
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PMID: 18082623
PDF is available here.
PDF is available here.
Microbiology (154)Pt 1 2008
Abstract
These results provide experimental evidence differentiating widely conserved Asp and His Box residues that are essential for function from those whose roles are modulated by specific local environments....
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PMID: 18174131
PDF is available here.
PDF is available here.
Genome Biology (9)4 2008
Abstract
SUMMARY: The AAA+ superfamily is a large and functionally diverse superfamily of NTPases that are characterized by a conserved nucleotide-binding and catalytic module, the AAA+ module. Members are involved in an astonishing range of different cellular processes, attaining this functional diversity t...
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PMID: 18466635
PDF is available here.
PDF is available here.
Ukrainskij biokhimicheskij zhurnal/Ukrayins'kij biokhimichnij zhurnal/Ukrainian biochemical journal (80)1 2008
Abstract
Soluble nucleoside triphosphatase differing in its properties from all known proteins with NTPase activity was partially purified from bovine kidneys. The enzyme has pH optimum of 7.5, molecular mass of 60 kDa, as estimated by gel chromatography, and shows an absolute dependence on divalent metal io...
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PMID: 18710021
PDF is available here.
PDF is available here.
Neuroscience (150)4 2007
Abstract
We analyzed the expression of TNAP at the lateral ventricles of the adult and developing mouse brain. In the adult brain, TNAP was expressed by type B, type A and at least subsets of type C cells of the SVZ and throughout the rostral migratory stream. Almost 100% of the proliferating, Ki-67-positive...
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PMID: 18031938
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (25)6 2007
Abstract
NTPase-II gene was specifically amplified, and the homology of DNA sequence was 100% to that in the GenBank. SDS-PAGE showed that the recombinant NTPase protein with correct molecular weight was expressed highly in E.coli BL21 (DE3). Western blotting testified that the purified recombinant protein c...
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PMID: 18441888
PDF is available here.
PDF is available here.
Mutational analysis of a helicase motif-based RNA 5'-triphosphatase/NTPase from bamboo mosaic virus.
Abstract
The helicase-like domain of BaMV replicase possesses NTPase and RNA 5'-triphosphatase activities. In this study, mutational effects of the helicase signature motifs and residue L543 on the two activities were investigated. Either activity was inactivated by K643A-S644A, D702A, D730A, R855A, or L543P...
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PMID: 17585982
PDF is available here.
PDF is available here.
Life Sciences (81)15 2007
Abstract
We investigate the effect of in vivo (acute and chronic) and in vitro treatments with the antidepressant drugs on nucleotidases activities in rat blood serum. In acute treatment, nortriptyline decreased ATP hydrolysis (41%), but not altered ADP and AMP hydrolysis. In contrast, fluoxetine did not alt...
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PMID: 17889906
PDF is available here.
PDF is available here.
Abstract
We tested a small interfering RNA (siRNA) directed against the M1 plus-strand RNAs of reovirus strains Type 1 Lang (T1L) and Type 3 Dearing (T3D). The siRNA strongly suppressed mu2 expression by either strain and reduced infectious yields in a strain-dependent manner. This first strain difference wa...
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PMID: 17451769
PDF is available here.
PDF is available here.
Journal of General Virology (88)Pt 6 2007
Abstract
Analysis of the amino acid sequence of core protein muA of avian reovirus has indicated that it may share similar functions to protein mu2 of mammalian reovirus. Since mu2 displayed both nucleotide triphosphatase (NTPase) and RNA triphosphatase (RTPase) activities, the purified recombinant muA ( muA...
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PMID: 17485541
PDF is available here.
PDF is available here.
Abstract
The title compound (4) was synthesized by the reaction of ethyl 1-(2,3,5-tri-O-benzoyl-beta-d-ribofuranosyl)-5-formylimidazole-4-carboxylate with excess guanidine in ethanol at reflux. Compound 4 was evaluated in vitro against NTPases/helicases of four different viruses of the Flaviviridae family, i...
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PMID: 17289387
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)16 2007
Abstract
We have conducted a biochemical and structure-function analysis of a TTM protein (CthTTM) from the bacterium Clostridium thermocellum. CthTTM is a metal-dependent tripolyphosphatase and nucleoside triphosphatase; it is not an adenylate cyclase. We have identified 11 conserved amino acids in the tunn...
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PMID: 17303560
PDF is available here.
PDF is available here.
Biochemical Journal (403)1 2007
Abstract
Our results showed that, among these four proteins, only P42 exhibited ATPase activity. Biochemical characteristics--optimal conditions for activity, substrate specificities, and inhibiting effects by ATP analogues--of the recombinant P42 proteins were very similar to those of a putative ATPase spec...
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PMID: 17083328
PDF is available here.
PDF is available here.
Journal of Molecular Biology (367)3 2007
Abstract
We showed that the purified recombinant protein is active as a non-specific nucleoside triphosphatase, with k(cat)=7.6x10(-3) s(-1). The structural basis for the enzymatic activity of HCR-NTPase was further characterized by site-directed mutagenesis of the Walker B motif, which further contributes t...
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PMID: 17291528
PDF is available here.
PDF is available here.
Journal of Virology (81)6 2007
Abstract
We have analyzed the biochemical consequences of mutations that affect viral RNA synthesis in Semliki Forest virus temperature-sensitive (ts) mutants. Of the six mutations mapping in the multifunctional replicase protein nsP2, three were located in the N-terminal helicase region and three were in th...
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PMID: 17202213
PDF is available here.
PDF is available here.
Biochemical Journal (401)3 2007
Abstract
We show that, for CENTG1 (centaurin gamma-1) the GTPase-like domain has broader trinucleotide specificity. Alterations within the G4 motif of CENTG1 from the highly conserved NKXD found in typical GTPases to TQDR result in the loss of specificity, a lower affinity for the nucleotides and higher turn...
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PMID: 17037982
PDF is available here.
PDF is available here.
Abstract
We demonstrate the interaction of the non-structural protein 3 (NS3) of hepatitis C virus (HCV) with alkaloide tropolone (2-hydroxy-2,4,6-heptatriene-1-one) and its derivatives. The compounds were biochemically screened separately against the ATPase and helicase activities of HCV NS3. In the investi...
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PMID: 17542155
PDF is available here.
PDF is available here.
Acta Oncologica (46)5 2007
Abstract
We measured cell survival and the effect of FMdC, AZT and FMdC + AZT on deoxynucleotide triphosphate pool. FMdC results in a significant increase of radiosensitivity. The enhancement ratios (ER =surviving fraction irradiated cells/surviving fraction drug treated and irradiated cells), obtained by FM...
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PMID: 17562437
PDF is available here.
PDF is available here.
Archives of Virology (152)8 2007
Abstract
We cloned and expressed full-length CSFV NS3 protein (NS3FL) and its N-terminal truncated version (ntNS3) in E. coli. NTPase activities of the purified NS3FL and ntNS3 proteins and their reaction conditions were investigated. The results showed that CSFV NS3FL and ntNS3 proteins contained a specific...
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PMID: 17447110
PDF is available here.
PDF is available here.
Human Reproduction (21)12 2006
Abstract
NALP14 was, as are the three other genes in 11p15, exclusively expressed in testis. Within the testis, the NALP14 protein was mainly expressed in A dark spermatogonia, mid and late spermatocytes and spermatids. The mutation screen revealed five mutations that occurred only in the patient group. One...
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PMID: 16931801
PDF is available here.
PDF is available here.
Journal of Molecular Biology (362)3 2006
Histidine triad-like motif of the rotavirus NSP2 octamer mediates both RTPase and NTPase activities.
Abstract
We investigated the possibility that the RNA-binding activity of the groove promoted the insertion of the 5'-triphosphate moiety of the RNA into the cleft, and the subsequent hydrolysis of its gamma-beta phosphoanhydride bond. Our results show that NSP2 hydrolyzes the gammaP from RNAs and NTPs throu...
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PMID: 16934294
PDF is available here.
PDF is available here.
International Journal for Parasitology (36)10-11 2006
Abstract
A putative nucleoside triphosphate hydrolase (NTPase) gene was identified in a database of expressed sequence tags (ESTs) from the apicomplexan parasite Sarcocystis neurona. Analysis of culture-derived S. neurona merozoites demonstrated a dithiol-dependent NTPase activity, consistent with the presen...
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PMID: 16790243
PDF is available here.
PDF is available here.
Abstract
We probe the active site of Plasmodium falciparum RNA triphosphatase by targeted mutagenesis and thereby identify eight residues essential for catalysis. The functional data engender an improved structural alignment in which the Plasmodium counterparts of the Cet1 tunnel strands and active-site func...
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PMID: 16809816
PDF is available here.
PDF is available here.
Journal of Virology (80)16 2006
Abstract
We demonstrate that NSP2 of group C rotavirus, a group that fails to reassort with group A viruses, retains the unique architecture of the group A octamer but differs in surface charge distribution. By using an NSP2-dependent complementation system, we show that the HIT-dependent NTPase activity of...
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PMID: 16873255
PDF is available here.
PDF is available here.
Abstract
I cells. In agreement with this observation, NTPDase2 was located to the same membrane as GLAST, indicating that this enzyme is present in type I cells. The presence of ecto-ATPase in taste buds likely reflects the importance of ATP as an intercellular signaling molecule in this system....
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PMID: 16680780
PDF is available here.
PDF is available here.
Glycoconjugate Journal (23)3-4 2006
Abstract
Our laboratory reported earlier a significant increase of PBR density in the nucleus of DMBA-induced malignant submandibular glands of rats, suggesting a role of PBR in nuclear events of peripheral tissues. Since then numerous studies have demonstrated the abundance of PBR in tumors. Numerous studie...
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PMID: 16691503
PDF is available here.
PDF is available here.
Molecular Microbiology (59)1 2006
Abstract
We discuss the structural diversity of these enzymes, their phylogenetic distribution, substrate specificity and the problem of identifying their true substrates....
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PMID: 16359314
PDF is available here.
PDF is available here.
Journal of Biomolecular NMR (36 Suppl 1) 2006
Abstract
Abstract
We designated as pAc3249A, pAc3249B and pAc3249C. We investigated and report here the existence and genetic organization of a Conjugal Type IV Secretion System (TFSS) in pAc3249A. METHODOLOGY/PRINCIPLE FINDING: The complete operon is 11,061 bp in length and has G+C content of 47.20% code for 12 ORFs...
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PMID: 17205119
PDF is available here.
PDF is available here.
Abstract
We have solved the structure of an enzymatically active fragment of the dengue virus NTPase/ helicase C-terminal catalytic domain in several related crystal forms. The structure is composed of three domains, bears an asymmetric distribution of charges and comprises a tunnel large enough to accommoda...
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PMID: 17319156
PDF is available here.
PDF is available here.
Nucleic Acids Research (34)1 2006
Abstract
The human gene ddx42 encodes a human DEAD box protein highly homologous to the p68 subfamily of RNA helicases. In HeLa cells, two ddx42 poly(A)+ RNA species were detected both encoding the nuclear localized 938 amino acid Ddx42p polypeptide. Ddx42p has been heterologously expressed and its biochemic...
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PMID: 16397294
PDF is available here.
PDF is available here.
Abstract
We demonstrate that Fap7 is strictly required for cleavage of the 20S pre-rRNA at site D in the cytoplasm. Genetic depletion of Fap7 causes accumulation of only the 20S pre-rRNA, which could be detected not only in 43S preribosomes but also in 80S-sized complexes. Fap7 is not a structural component...
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PMID: 16287850
PDF is available here.
PDF is available here.
Journal of Virology (79)21 2005
Abstract
We tested mutations in the conserved helicase motifs of 1a to further define the roles of this domain in RNA template recruitment. All 1a helicase mutations tested showed normal 1a accumulation, localization to perinuclear endoplasmic reticulum membranes, and recruitment of 2a(pol). Most 1a helicase...
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PMID: 16227294
PDF is available here.
PDF is available here.
FEBS Letters (579)22 2005
Abstract
We report that the CPs of two filamentous RNA viruses, potato virus X (PVX, Potexvirus) and potato virus A (PVA, Potyvirus) exhibit an enzyme activity. The CP isolated from PVX virions possesses ATP-binding and ATPase activities. Recombinant PVX and PVA CPs produced in Escherichia coli show Mg2+-dep...
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PMID: 16115626
PDF is available here.
PDF is available here.
Toxicology (212)2-3 2005
Abstract
We verified the effect of exposure in vitro and in vivo to carbofuran and malathion on ectonucleotidase activities from brain membranes of zebrafish. To verify if carbofuran and malathion have a direct inhibitory effect on NTPDase and 5'-nucleotidase activities in brain membranes of zebrafish, we ha...
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PMID: 15951093
PDF is available here.
PDF is available here.
Journal of Virology (79)16 2005
Abstract
We present the structure of an enzymatically active fragment of the Dengue virus NTPase/helicase catalytic domain to 2.4 A resolution. The structure is composed of three domains, displays an asymmetric distribution of charges on its surface, and contains a tunnel large enough to accommodate single-s...
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PMID: 16051821
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (280)29 2005
Abstract
Dengue virus type 2 (DEN2), a member of the Flaviviridae family, is a re-emerging human pathogen of global significance. DEN2 nonstructural protein 3 (NS3) has a serine protease domain (NS3-pro) and requires the hydrophilic domain of NS2B (NS2BH) for activation. NS3 is also an RNA-stimulated nucleos...
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PMID: 15917225
PDF is available here.
PDF is available here.
Biochemistry (Washington) (44)28 2005
Abstract
We characterize the RNA-binding, NTP hydrolysis, and RNA unwinding functions of Prp22. Using nitrocellulose filter binding assays, we determined that the apparent affinity of Prp22 is approximately 20-fold greater for single-stranded RNA than for single-stranded DNA or duplex nucleic acids. Inclusio...
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PMID: 16008364
PDF is available here.
PDF is available here.
Neurological Research (27)4 2005
Abstract
Numbers of apoptotic cells increased significantly (p<0.0001) from 3 to 8 hours after exposure, but returned to baseline levels by 24 hours. Little evidence of apoptosis was observed in non-irradiated chiasms. Similar patterns of increase in apoptotic rate were observed in the genu of the corpus cal...
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PMID: 15949230
PDF is available here.
PDF is available here.
Biochemistry (Washington) (44)21 2005
Abstract
Previous work has demonstrated that Escherichia coli DbpA is a nonprocessive RNA helicase that can disrupt short RNA helices on either the 5' side or 3' side of hairpin 92 of 23S rRNA. Here the directionality of the helicase activity of DbpA was determined by using substrates containing a short repo...
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PMID: 15910005
PDF is available here.
PDF is available here.
Abstract
We hypothesized that altered nucleotide concentrations in HPRT deficiency change G-protein-mediated signal transduction. Therefore, our original study aim was to examine the high-affinity GTPase activity of G-proteins in membranes from primary human skin and immortalized mouse skin fibroblasts, rat...
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PMID: 15935074
PDF is available here.
PDF is available here.
Developmental Brain Research (156)1 2005
Abstract
We did find increased MBP protein expression by Western blotting. ZO-1 protein and DM20 mRNA levels were increased during the course of differentiation and after IGF-I administration. The present findings suggest that ZO-1 may be used as a marker for OLN-93 oligodendroglia differentiation....
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PMID: 15862630
PDF is available here.
PDF is available here.
Biology of Reproduction (72)4 2005
Abstract
Microscopy of sectioned neonatal mouse ovaries established the predominance of primordial follicles in Day 3 samples and the predominance of primary follicles by Day 8. To identify genetic determinants of the primordial to primary follicle transition, the transcriptome of Day 1 or Day 3 mouse ovarie...
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PMID: 15590904
PDF is available here.
PDF is available here.
Psychiatry Research (138)1 2005
Abstract
We examined how lithium's demonstrated effects on various cellular processes in human brain would be reflected in the (31)P magnetic resonance spectra of living human beings with respect to brain high-energy phosphate metabolites. Eight healthy volunteers received a baseline (31)P magnetic resonance...
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PMID: 15708301
PDF is available here.
PDF is available here.
Abstract
Abstract
To improve anti-helical activity of analogues of 1H-benzotriazole and 1H-benzimidazole their N-alkyl derivatives were synthesized and tested for antihelicase activity against enzymes of selected Flaviviridae including hepatitis C virus (HCV), West Nile virus (WNV), Dengue virus (DENV) and Japanese e...
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PMID: 16245647
PDF is available here.
PDF is available here.
Nucleic Acids Research (33)15 2005
Abstract
We also demonstrate that PstII and EcoP15I cannot interact and cleave a DNA substrate suggesting that Type III enzymes must make specific protein-protein contacts to activate endonuclease activity....
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PMID: 16120967
PDF is available here.
PDF is available here.
Acta poloniae pharmaceutica (61 Suppl) 2004
Abstract
Abstract: In the search for inhibitors of the non-structural protein 3 (NS3)-associated NTPase/helicase activities of the hepatitis C virus (HCV), and of the related West Nile Virus (WNV), and Japanese Encephalitis Virus (JEV), random screening of a broad range of unrelated low-molecular weight comp...
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PMID: 15909930
PDF is available here.
PDF is available here.