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Thiamin-Triphosphatase (0):
Recent article
Thiamin-Triphosphatase
Ukrainskij biokhimicheskij zhurnal/Ukrayins'kij biokhimichnij zhurnal/Ukrainian biochemical journal (82)1 2010
Abstract
Our results allow us to suppose that different active protein sites are responsible for the specific binding and for thiamine phosphates hydrolysis by TBP of synaptic membranes....
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PMID: 20684226
PDF is available here.
PDF is available here.
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Abstract
We report substantial improvements to the previously introduced automated NOE assignment and structure determination protocol known as PASD (Kuszewski et al. (2004) J Am Chem Soc 26:6258-6273). The improved protocol includes extensive analysis of input spectral data to create a low-resolution contac...
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PMID: 18668206
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)16 2008
Abstract
We have investigated the kinetic properties of recombinant mouse thiamine triphosphatase (mThTPase) and determined its solution structure by NMR spectroscopy. Residues responsible for binding Mg(2+) and ThTP were determined from NMR titration experiments. The binding of Mg(2+) induced only a minor l...
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PMID: 18276586
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1725)1 2005
Abstract
We have recently cloned the cDNA coding for a highly specific mammalian 25-kDa thiamine triphosphatase. The enzyme was active in all mammalian species studied except pig, although the corresponding mRNA was present. In order to determine whether the very low ThTPase activity in pig tissues is due to...
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PMID: 16000236
PDF is available here.
PDF is available here.
Abstract
Thiamine triphosphate (ThTP) is found in most organisms, but its biological role remains unclear. In mammalian tissues, cellular ThTP concentrations remain low, probably because of hydrolysis by a specific 25 kDa thiamine triphosphatase (ThTPase). The aim of the present study was to use quantitative...
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PMID: 15203116
PDF is available here.
PDF is available here.
Abstract
We have recently cloned the cDNA coding for a highly specific mammalian 25 kDa thiamine triphosphatase (ThTPase; EC 3.6.1.28). As the enzyme has a high catalytic efficiency and no sequence homology with known phosphohydrolases, it was worth investigating its structure and catalytic properties. For t...
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PMID: 15109578
PDF is available here.
PDF is available here.
Neuroscience (125)4 2004
Abstract
We have recently characterized a highly specific 25-kDa thiamine triphosphatase (ThTPase) that is expressed in most mammalian tissues. The role of this enzyme may be the control of intracellular concentrations of ThTP. As the latter has been considered to be a neuroactive form of thiamine, we have s...
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PMID: 15120844
PDF is available here.
PDF is available here.
Abstract
We show that ThTP exists not only in vertebrates but is present in bacteria, fungi, plants and invertebrates. Unexpectedly, we found that in Escherichia coli as well as in Arabidopsis thaliana, ThTP was synthesized only under particular circumstances such as hypoxia (E. coli) or withering (A. thalia...
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PMID: 12943234
PDF is available here.
PDF is available here.
Ukrainskij biokhimicheskij zhurnal/Ukrayins'kij biokhimichnij zhurnal/Ukrainian biochemical journal (75)5 2003
Abstract
These results imply the existence of ThTPase multiple forms in mammalian cells....
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PMID: 14681994
PDF is available here.
PDF is available here.
Ukrainskij biokhimicheskij zhurnal/Ukrayins'kij biokhimichnij zhurnal/Ukrainian biochemical journal (75)6 2003
Abstract
The distribution of thiamine-binding and thiamine triphosphatase activity typical of thiamine-binding proteins was studied in intracellular structures of rats liver and kidneys. It was found that the fraction of microsomes has the highest rate of specific thiamine-binding activity amide fractions of...
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PMID: 15143527
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (277)16 2002
Abstract
We purified a soluble thiamine triphosphatase (ThTPase; EC ) from calf brain. The bovine ThTPase is a 24-kDa monomer, hydrolyzing ThTP with virtually absolute specificity. Partial sequence data obtained from the purified bovine enzyme by tandem mass spectrometry were used to search the GenBank data...
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PMID: 11827967
PDF is available here.
PDF is available here.
Abstract
Properties of soluble thiamine triphosphatase (ThTPase), adenosine triphosphatase, nucleoside triphosphatase and alkaline phosphatase activities in bovine kidney were compared. ThTPase and the other phosphatases differed clearly in their pH-dependences, K(m) and molecular masses. Apparent K(m) and p...
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PMID: 9784846
PDF is available here.
PDF is available here.
Ukrainskij biokhimicheskij zhurnal/Ukrayins'kij biokhimichnij zhurnal/Ukrainian biochemical journal (70)1 1998
Abstract
The analysis of the steady-state kinetics of the thiamine triphosphate ester hydrolysis reaction catalyzed by homogeneous thiamine triphosphatase (EC 3.6.1.28; thiamine triphosphate phosphohydrolase) from bovine brain enables us to suggest, that the ThTP binding to the catalytic site of the ThTPase...
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PMID: 9848135
PDF is available here.
PDF is available here.
Ukrainskij biokhimicheskij zhurnal/Ukrayins'kij biokhimichnij zhurnal/Ukrainian biochemical journal (69)5-6 1997
Abstract
The steady-state kinetics of the ThTP hydrolysis by thiamine triphosphatase (EC 3.6.1.28) from bovine brain testified to the presence of two kinetically significant conformational states of the protein, their equilibrium being determined by the substrate concentration. The ThTPase isomeric forms had...
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PMID: 9606824
PDF is available here.
PDF is available here.
Annals of Neurology (39)5 1996
Abstract
We measured directly levels of free (nonphosphorylated) thiamine and its phosphate esters, thiamine monophosphate and thiamine diphosphate (TDP), and activities of three TDP-metabolizing enzymes (thiamine pyrophosphokinase, thiamine diphosphatase, and thiamine triphosphatase) in autopsied cerebral c...
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PMID: 8619543
PDF is available here.
PDF is available here.
Abstract
Total thiamine (the sum of thiamine and its phosphate esters) concentrations are two- to fourfold lower in human brain than in the brain of other mammals. There were no differences in the total thiamine content between biopsied and autopsied human brain, except that in the latter, thiamine triphosph...
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PMID: 8522961
PDF is available here.
PDF is available here.
Abstract
Our results show that a net synthesis of thiamine triphosphate (TTP) can be demonstrated in vitro using rat brain extracts. The total homogenate was preincubated with thiamine or its diphosphate derivative (TDP), centrifuged, and washed twice. With TDP (1 mM) as substrate, a 10-fold increase in TTP...
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PMID: 8380431
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1117)3 1992
Abstract
Soluble thiamine triphosphatase (EC 3.6.1.28) of bovine brain has been purified 68,000-fold to an electrophoretically homogeneous state with an overall recovery of 5.5% by hydrophobic chromatography on Toyopearl HW-60, Sephadex G-75 gel filtration, DEAE-Toyopearl 650M chromatography and Blue Sepharo...
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PMID: 1329971
PDF is available here.
PDF is available here.
Abstract
Journal of Chromatography A (566)2 1991
Abstract
Our results suggest that this compound could play an important role in the regulation of chloride permeability....
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PMID: 1658022
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1073)1 1991
Abstract
The membrane-associated, anion-regulated thiamine triphosphatase from Electrophorus electricus electric organ can be solubilized by various neutral detergents. Polyoxyethylene ethers are the most effective. Anionic detergents readily inactivate the enzyme. A 6.4-fold increase in specific activity is...
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PMID: 1846755
PDF is available here.
PDF is available here.
Abstract
1. The activities of 2-oxoglutarate dehydrogenase, transketolase, thiamine pyrophosphokinase and thiamine triphosphatase and the concentrations of thiamine phosphates were almost the same between rat extensor digitorum longus and soleus muscles at 2 weeks of age. 2. These enzyme activities changed a...
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PMID: 1847884
PDF is available here.
PDF is available here.
Abstract
1. Thiamine triphosphatase activity in particulate fraction, but not in soluble, of rat skeletal muscle was stimulated by several anions. 2. The stimulative effect of anions was dependent on pH of reaction medium and was reversible. 3. The activities of ATPase in rat muscle particulate preparation a...
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PMID: 1664809
PDF is available here.
PDF is available here.
Abstract
Thiamine and thiamine mono-, pyro- and triphosphate were found at detectable levels in synaptosomes isolated from whole rat brain. Synaptosomes prepared from whole brain, cerebellum and medulla were also found to contain uridine and inosine mono- and diphosphatases as well as the thiamine pyrophosph...
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PMID: 1963298
PDF is available here.
PDF is available here.
Abstract
Our data suggest the existence of a high-affinity binding site (KD of approximately 0.5 microM) for the Mg-TTP complex.(ABSTRACT TRUNCATED AT 250 WORDS)...
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PMID: 2547899
PDF is available here.
PDF is available here.
Abstract
The activities of thiamine diphosphatase (TDPase), thiamine triphosphatase (TTPase), and thiamine pyrophosphokinase and the contents of thiamine and its phosphate esters were determined in rat brain cortex, cerebellum, and liver from birth to adulthood. Microsomal TTPase activity in the cerebral cor...
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PMID: 2537379
PDF is available here.
PDF is available here.
Abstract
Thiamine triphosphatase (TTPase) from membranes isolated from the main electric organ of E. electricus is activated about 8 fold by NO3-, I- and SCN- while SO42- is inhibitory. Activating anions shift the pH optimum of the enzyme from 5.0 to 8.0. The enzyme is irreversibly inactivated by low concent...
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PMID: 2841936
PDF is available here.
PDF is available here.
Abstract
The main electric organ of Electrophorus electricus is particularly rich in thiamine triphosphate, which represents 87% of the total thiamine content in this tissue. The thiamine pyrophosphate concentration, however, is very low in the eel electric organ and skeletal muscle as compared with other ee...
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PMID: 3037030
PDF is available here.
PDF is available here.
Abstract
Thiamin triphosphate (ThTP) in early stationary phase cells of Escherichia coli grown in nutrient broth with 0.1% yeast extract was found to constitute approximately 5-7% of cellular thiamin diphosphate (ThDP) or around 5 nmol/g cell. Nearly the same level of ThTP was obtained in a Bacillus strain....
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PMID: 3039089
PDF is available here.
PDF is available here.
Abstract
A membrane-bound nonspecific triphosphatase of E. coli was solubilized and purified to a homogeneous SDS-acrylamide gel electrophoresis band. It was found to be a single polypeptide of 16 kDa requiring no Mg2+, with an optimal pH at 6.5. The substrate specificity was broad and a nonspecific Mg2+-ind...
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PMID: 3026493
PDF is available here.
PDF is available here.
Abstract
Neurochemical Research (10)6 1985
Abstract
These results suggest that thiamine triphosphate is synthesized in vivo in rat brain....
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PMID: 2993937
PDF is available here.
PDF is available here.
Rinsho Shinkeigaku (23)12 1983
Abstract
Abstract
Medical biology (59)3 1981
Abstract
Polyacrylamide gel electrophoresis was used to investigate the relation of the soluble thiamine triphosphatase activity of various rat tissues to other phosphatases. This technique separated the thiamine triphosphatase of rat brain, heart, kidney, liver, lung, muscle and spleen from alkaline phospha...
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PMID: 6273668
PDF is available here.
PDF is available here.
Analytical Biochemistry (102)2 1980
Abstract
Abstract