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Transposases (1):
Recent article
Transposases
Molecular Therapy (18)10 2010
Abstract
We compared the genomic integration efficiencies and transposition site preferences of Sleeping Beauty (SB or SB11), Tol2, and piggyBac (PB) transposon systems in primary T cells derived from peripheral blood lymphocytes (PBL) and umbilical cord blood (UCB). We found that PB demonstrated the highest...
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PMID: 20606646
PDF is available here.
PDF is available here.
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Abstract
We found a novel piggyBac-like DNA transposon that potentially encodes an intact transposase (610 amino acid residues), which is flanked by 16-bp perfect inverted terminal repeats and a duplicated TTAA target site. Interestingly, we also identified another intact copy of this transposon on an autoso...
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PMID: 20725145
PDF is available here.
PDF is available here.
Genetics Research (92)4 2010
Abstract
TP5, a P element inserted in the telomere-associated sequences of the X chromosome, represses the excision of other P elements in the germ line through a combination of maternal and zygotic effects. The maternal component of this repression is impaired by heterozygous mutations in the aubergine and...
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PMID: 20943007
PDF is available here.
PDF is available here.
Abstract
We have discovered that the cryptic endonuclease activity reported for the isolated C-terminal domain of the transposase MuA [Wu Z, Chaconas G (1995) A novel DNA binding and nuclease activity in domain III of Mu transposase: Evidence for a catalytic region involved in donor cleavage. EMBO J 14:3835-...
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PMID: 20167799
PDF is available here.
PDF is available here.
Abstract
We set out to synthesize what is currently known about Mos1 insertion sites, and to define the characteristics of Mos1 insertion sequences in vitro and in vivo. Statistical analysis can be used to identify the TA dinucleotides that are non-randomly targeted for transposon integration. In vitro, no s...
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PMID: 19629719
PDF is available here.
PDF is available here.
Abstract
We review the use of viruses and the limitations of current approaches to gene therapy, followed by a more detailed analysis of transposon length and the physical properties of internal sequences, which both affect transposition efficiency. As transposon length increases, transposition decreases: th...
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PMID: 20084428
PDF is available here.
PDF is available here.
Abstract
Tc1, one of the founding members of the Tc1/mariner transposon superfamily, was identified in the nematode Caenorhabditis elegans more than 25 years ago. Over the years, Tc1 and other endogenous mariner transposons became valuable tools for mutagenesis and targeted gene inactivation in C. elegans. H...
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PMID: 19347589
PDF is available here.
PDF is available here.
Abstract
We termed the protein Metnase (also called SETMAR). Metnase methylates histone H3 lysine 36 (H3K36), improves the integration of foreign DNA, and enhances DNA double-strand break (DSB) repair by the non-homologous end joining (NHEJ) pathway, potentially dependent on its interaction with DNA Ligase I...
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PMID: 20309721
PDF is available here.
PDF is available here.
Abstract
In the last 20 years, tools derived from DNA transposons have made major contributions to genetic studies from gene delivery to gene discovery. Various complementary and fairly ubiquitous DNA vehicles have been developed. Although many transposons are efficient DNA vehicles, they appear to have limi...
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PMID: 19662501
PDF is available here.
PDF is available here.
Abstract
The mariner-like transposon Mos1 is used for insertional mutagenesis and transgenesis in different animals (insects, nematodes), but has never been used in plants. In this paper, the transposition activity of Mos1 was tested in Nicotiana tabacum, but no transposition event was detected. In an attemp...
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PMID: 19847655
PDF is available here.
PDF is available here.
Abstract
We summarize the current mariner-based transposon tools and techniques for conducting genetic studies in bacteria....
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PMID: 19757097
PDF is available here.
PDF is available here.
Abstract
Mariners are small DNA mediated transposons of eukaryotes that fortuitously function in bacteria. Using bacterial genetics, it is possible to study a variety of properties of mariners, including transpositional ability, dominant-negative regulation, overexpresson inhibition, and the function of cis-...
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PMID: 19711186
PDF is available here.
PDF is available here.
Abstract
The stable introduction of therapeutic transgenes into human cells can be accomplished using viral and nonviral approaches. Transduction with clinical-grade recombinant viruses offers the potential of efficient gene transfer into primary cells and has a record of therapeutic successes. However, wide...
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PMID: 20104209
PDF is available here.
PDF is available here.
Human Gene Therapy (21)4 2010
Abstract
We apply PB transposition to express a chimeric antigen receptor (CAR) in primary human T cells. We demonstrate that T cells electroporated to introduce the PB transposon and transposase stably express CD19-specific CAR and when cultured on CD19(+) artificial antigen-presenting cells, numerically ex...
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PMID: 19905893
PDF is available here.
PDF is available here.
Eukaryotic Cell (9)3 2010
Abstract
Genes that are essential for viability represent potential targets for the development of anti-infective agents. However, relatively few have been determined in the filamentous fungal pathogen Aspergillus fumigatus. A novel solution employing parasexual genetics coupled with transpos...
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PMID: 20097738
PDF is available here.
PDF is available here.
Abstract
We briefly review biochemical and mechanistic aspects of DNA transposition, and propose that integrating sequence information with structural information using bioinformatics tools such as secondary structure prediction and protein threading can lead not only to an additional level of understanding...
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PMID: 20067338
PDF is available here.
PDF is available here.
Abstract
We developed a novel mariner-based transposon system for in vivo random mutagenesis of C. difficile R20291, the BI/NAP1/027 epidemic strain at the center of the C. difficile outbreaks in Stoke Mandeville, United Kingdom, in 2003 to 2004 and 2004 to 2005. Transposition occurred at a frequency of 4.5...
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PMID: 20023081
PDF is available here.
PDF is available here.
Tsitologiia (52)6 2010
Abstract
The distribution patterns pf hobo transposon and Dm412 retrotransposon hybridization sites on the salivary gland polytene chromosomes from the larvae of Drosophila melanogaster isogenic strain 51, used for analyzing the effect of transposable element (TE) transposition on the selection for quantitat...
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PMID: 20737907
PDF is available here.
PDF is available here.
Abstract
We studied cassava (Manihot esculenta) En/Spm-like transposons (Meens) with regard to genomic distribution, sequence diversity and methylation status. Cassava transposase fragments characteristic of En/Spm-like transposons were isolated, cloned and characterized. Sequence analysis showed that cassav...
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PMID: 20449796
PDF is available here.
PDF is available here.
Abstract
We describe methodology for creating and characterizing functional chimeric ZF transposases and experimental approaches for testing their transpositional activity in human cells....
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PMID: 20680846
PDF is available here.
PDF is available here.
Genes & Development (24)1 2010
Abstract
Programmed DNA rearrangements are critical for the development of many organisms and, intriguingly, can be catalyzed by domesticated mobile genetic elements. In this issue of Genes & Development, Barsoum and colleagues (pp. 33-44) demonstrate that, in the budding yeast Kluyveromyces lactis, a DNA re...
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PMID: 20047997
PDF is available here.
PDF is available here.
Genes & Development (24)1 2010
Abstract
We demonstrate that a protein homologous to transposases, called alpha3, was essential for mating type switch in Kluyveromyces lactis. Mutational analysis showed that amino acids conserved among transposases were essential for its function. During switching, sequences in the 5' and 3' flanking regio...
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PMID: 20008928
PDF is available here.
PDF is available here.
Abstract
We describe an optimized mammalian germ line transposition system active during early mouse spermatogenesis using the Minos transposon. Transposon-positive progeny carry on average more than 2 new transpositions, and 45 to 100% of the progeny carry an insertion in a gene. The optimized Minos-based s...
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PMID: 19884347
PDF is available here.
PDF is available here.
Genetics Research (91)5 2009
Abstract
Strains carrying the X-linked telomeric P elements TP5 or TP6 varied in their ability to repress hybrid dysgenesis. The rank ordering of these strains was consistent across different genetic assays and was not related to the type of telomeric P element (TP5 or TP6) present. Strong repression of dysg...
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PMID: 19922696
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)30 2009
Abstract
We report the biochemical study of two copies of the Pacmmar element (Pacmmar1.1 and Pacmmar1.2), isolated from the coastal crab Pachygrapsus marmoratus. These two copies present an open reading frame encoding a putative active transposase. Using an in vitro transposition assay, we show that Pacmmar...
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PMID: 19530701
PDF is available here.
PDF is available here.
Gene Therapy (16)6 2009
Abstract
We hypothesize that co-delivery of the genes for FVIII and IDO can attenuate inhibitor formation. Using transposon-based gene delivery, we observed long-term therapeutic FVIII expression and significantly reduced inhibitor titers when the genes were co-delivered. Co-expression of FVIII and IDO in th...
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PMID: 19262614
PDF is available here.
PDF is available here.
Genes & Development (23)9 2009
Abstract
A recent study by Zhang and colleagues published in the March 15, 2009, issue of Genes & Development (pp. 755-765) demonstrates that maize Ac/Ds transposons mediate translocations and other rearrangements through aberrant execution of the normal transposition process. Ac transposase uses one end fro...
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PMID: 19417101
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (294)2 2009
Abstract
Insertion sequences (ISs) are mobile genetic elements widely distributed among bacteria. Their impact on the bacterial genome is multifold, including transfer of genetic information, shuttle of adaptive traits and influence on the genomic content. As a result, ISs play an important role in the organ...
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PMID: 19416360
PDF is available here.
PDF is available here.
Nature Methods (6)5 2009
Abstract
We describe an efficient piggyBac transposon-based approach to generate integration-free iPSCs. Transposons carrying 2A peptide-linked reprogramming factors induced reprogramming of mouse embryonic fibroblasts with equivalent efficiencies to retroviral transduction. We removed transposons from these...
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PMID: 19337237
PDF is available here.
PDF is available here.
Abstract
We have investigated the properties of a suspected hotspot for the integration of the mariner Mos1 element, namely the Tn9 cat gene that encodes a chloramphenicol acetyl transferase. Using in vitro and bacterial transposition assays, we confirmed that the cat gene is a preferential target for MOS1 i...
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PMID: 19112581
PDF is available here.
PDF is available here.
Abstract
Mobile genetic segments, or transposons, are also referred to as "jumping genes" as they can shift from one position in the genome to another, thus inducing a chromosomal mutation. According to the target site-specificity of the transposon during a transposition event, the result is either the inser...
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PMID: 19349746
PDF is available here.
PDF is available here.
EMBO Reports (10)2 2009
Abstract
I present RISF proteins from a structural perspective, describing the individual members and the common and divergent elements of their structures, as well as the mechanistic insights gained from the structures of RNase H1 enzyme complexes with RNA/DNA hybrids....
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PMID: 19165139
PDF is available here.
PDF is available here.
Abstract
We describe efforts to generate a transposon-based mutant collection of soybean. The Ds transposon system was used to create activation-tagging, gene and enhancer trap elements. Currently, the repository houses approximately 900 soybean events, with flanking sequence data derived from 200 of these e...
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PMID: 18855007
PDF is available here.
PDF is available here.
BMC Microbiology (9)1 2009
Mobile genetic elements in the genome of the beneficial rhizobacterium Pseudomonas fluorescens Pf-5.
Abstract
Sequence analyses revealed that the genome of Pf-5 is devoid of transposons and IS elements and that mobile genetic elements (MGEs) are represented by prophages and genomic islands that collectively span over 260 kb. The prophages include an F-pyocin-like prophage 01, a chimeric prophage 03, a lambd...
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PMID: 19144133
PDF is available here.
PDF is available here.
BMC Genomics (10)1 2009
Abstract
The 1.57 Mbp genome of the hyperthermophilic crenarchaeote Staphylothermus marinus has been completely sequenced. The main energy generating pathways likely involve 2-oxoacid:ferredoxin oxidoreductases and ADP-forming acetyl-CoA synthases. S. marinus possesses several enzymes not present in other cr...
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PMID: 19341479
PDF is available here.
PDF is available here.
Genome Biology (10)4 2009
Abstract
We present a large-scale study of the distributions of transposases across phylogeny and habitat, and find a significant correlation between habitat and transposase connections. We observed cases where phylogenetic boundaries are traversed, especially when organisms share habitats; this suggests tha...
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PMID: 19393086
PDF is available here.
PDF is available here.
Journal of Heredity (100)4 2009
Abstract
We determine the distribution of Hermes in populations of M. domestica as well as whether Hermes transposase has undergone similar sequence expansions and contractions during its evolution in this species. Hermes transposons were found in all M. domestica individuals sampled from 14 populations coll...
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PMID: 19366812
PDF is available here.
PDF is available here.
Abstract
We describe here a DNA transposon-based platform that permits us to explore the oncogenic potential of genetic mutations in the mouse. Briefly, promoter-less human cancer gene cDNAs were first cloned into Sleeping Beauty (SB) transposons. DNA transposition in the mouse that carried both the transpos...
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PMID: 19064922
PDF is available here.
PDF is available here.
Abstract
We report the in vivo Tol2 transposition system in a model vertebrate, zebrafish. First, we constructed transgenic zebrafish that carried single-copy integrations of Tol2 on the genome and injected transposase mRNA into one-cell stage embryos. The Tol2 insertions were mobilized efficiently in the ge...
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PMID: 19060204
PDF is available here.
PDF is available here.
Veterinary Microbiology (132)3-4 2008
Abstract
An insertion sequence (IS), designated ISApl1, was identified in Actinobacillus pleuropneumoniae. It was 1072 bp in length, and contained a large open reading frame (ORF), which encoded a putative transposase whose sequence was similar to that of transposases of various IS elements of the IS30 famil...
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PMID: 18632228
PDF is available here.
PDF is available here.
Journal of Molecular Biology (382)3 2008
Abstract
We discovered a mariner-family transposon, Mboumar, in the satellite DNA of the ant Messor bouvieri. Several copies of the transposon contain a full-length open reading frame, including Mboumar-9, which has 64% nucleotide identity to Mos1 of Drosophila mauritiana. To determine whether Mboumar is cur...
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PMID: 18675277
PDF is available here.
PDF is available here.
Journal of Cell Biology (183)1 2008
Abstract
We identify Sln as a novel LKB1-interacting protein using Drosophila melanogaster genetic modifier screening. Sln expression does not affect cell cycle progression or cell size but specifically enhances LKB1-dependent apoptosis and tissue size reduction. Conversely, down-regulation of Sln suppresses...
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PMID: 18838551
PDF is available here.
PDF is available here.
Acta Microbiologica Sinica (48)10 2008
Abstract
We found that among 94 V. alginolyticus strains, only V. alginolyticus E0601 and E0612, from east coastal areas of Guangdong province, produced predicted positive amplification fragments in PCR detection. Sequencing indicated that amplification fragments from V. alginolyticus E0601 and E0612 had ide...
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PMID: 19160819
PDF is available here.
PDF is available here.
Abstract
We recently isolated from the same patient tdh+ strains and a tdh(-) strain (PCR examination) that were otherwise indistinguishable. The purpose of this study was to examine the hypothesis that the tdh(-) strain was derived from the tdh+ strain by a deletion of the tdh gene mediated by a functional...
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PMID: 18598741
PDF is available here.
PDF is available here.
Developmental Dynamics (237)9 2008
Abstract
We now demonstrate Tol1 germline transgenesis in zebrafish. A construct containing the green fluorescence protein (GFP) reporter gene inserted between the Tol1 arms was microinjected together with Tol1 transposase mRNA into fertilized eggs. Sustained GFP expression was observed in 88% of 1-month-old...
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PMID: 18729212
PDF is available here.
PDF is available here.
Abstract
We demonstrate that breaks are most prominently repaired by gene conversion from the homolog, but also rarely by nonhomologous end-joining. In some cases, gene conversion events are resolved by crossing over. Surprisingly, expression of the transposase using an intestine-specific promoter can induce...
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PMID: 18757928
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)18 2008
Abstract
We show here that the ends exhibit functional differences which, in vivo, may favor the use of one compared to the other during transposition. Electromobility shift assays showed that a truncated form of the transposase [OrfAB(1-149)] exhibits higher affinity for IRR than for IRL. While there was no...
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PMID: 18586933
PDF is available here.
PDF is available here.
Abstract
We generated a MuB variant that could select target DNA independently of ATP. This Arc-MuB fusion protein allowed us to test whether ATP binding and hydrolysis by MuB are necessary for stimulation of transposition into selected DNA, a process termed target delivery. We find that with the fusion prot...
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PMID: 18719126
PDF is available here.
PDF is available here.
Abstract
The telomeric P elements TP5 and TP6 are associated with the P cytotype, a maternally inherited condition that represses P-element-induced hybrid dysgenesis in the Drosophila germ line. To see if cytotype repression by TP5 and TP6 might be mediated by the polypeptides they could encode, hobo transge...
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PMID: 18579507
PDF is available here.
PDF is available here.
Molecular Cell (31)1 2008
Abstract
We identify a class of 21 nucleotide RNAs, previously named 21U-RNAs, as the piRNAs of C. elegans. Piwi and piRNA expression is restricted to the male and female germline and independent of many proteins in other small-RNA pathways, including DCR-1. We show that Piwi is specifically required to sile...
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PMID: 18571451
PDF is available here.
PDF is available here.
Abstract
We characterized the properties of the PB transposon in mouse embryonic stem (ES) cells. We first measured the transposition efficiencies of PB transposon in mouse embryonic stem cells. We next constructed a PB/SB hybrid transposon to compare PB and Sleeping Beauty (SB) transposon systems and demons...
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PMID: 18579772
PDF is available here.
PDF is available here.
Abstract
A novel insertion sequence (IS), ISPst9, from Pseudomonas stutzeri AN10 was cloned and characterized. ISPst9 is a typical bacterial IS, consisting of a 2472-bp element flanked by 24-bp perfect inverted repeats that generates 8-bp AT-rich target duplications upon insertion. The sequence also contains...
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PMID: 18645960
PDF is available here.
PDF is available here.
Nucleic Acids Research (36)11 2008
Abstract
Nonviral integration systems are widely used genetic tools in transgenesis and play increasingly important roles in strategies for therapeutic gene transfer. Methods to efficiently regulate the activity of transposases and site-specific recombinases have important implications for their spatiotempor...
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PMID: 18499713
PDF is available here.
PDF is available here.
Genetics Research (90)3 2008
Abstract
We found homologous sequences in every species of the Drosophila melanogaster species subgroup. Some evidence suggests that these non-autonomous sequences were kept mobilizable for at least 0.4 million years. Also, some very short sequences with miniature inverted-repeat transposable element (MITE)...
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PMID: 18593511
PDF is available here.
PDF is available here.
Genome Research (18)5 2008
Abstract
We recently reported evidence for the relatively recent activity of hAT and Helitron elements, two distinct groups of DNA transposons, in the lineage of the vespertilionid bat Myotis lucifugus. Here, we describe seven additional families that have also been recently active in the bat lineage. Early...
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PMID: 18340040
PDF is available here.
PDF is available here.
Cancer Research (68)8 2008
Abstract
We and others have introduced a chimeric antigen receptor (CAR) to enable T cells to recognize lineage-specific tumor antigen, such as CD19, and early-phase human trials are currently assessing safety and feasibility. However, a significant barrier to next-generation clinical studies is developing a...
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PMID: 18413766
PDF is available here.
PDF is available here.
Molecular Cell (30)1 2008
Abstract
We explore how substrate multimerization modulates recognition by the ClpX unfoldase using a natural substrate, MuA transposase. MuA is initially monomeric but forms a stable tetramer when bound to transposon DNA. Destabilizing this tetramer by ClpX promotes an essential transition in the phage Mu r...
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PMID: 18406325
PDF is available here.
PDF is available here.
EMBO Journal (27)7 2008
Abstract
We have found that DNA synthesis can be avoided during piggyBac transposition, both at the donor site following transposon excision and at the insertion site following transposon integration. We demonstrate that piggyBac transposon excision occurs through the formation of transient hairpins on the t...
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PMID: 18354502
PDF is available here.
PDF is available here.
Abstract
We tested two experimental procedures to induce mobility of OPHIO TEs: (1) an exogenous (cloned) copy of OPHIO1 was introduced into the O. novo-ulmi subsp. americana strain W2 (OPHIO1 free strain) to give mutant strain W2:OPHIO1. After exposure of W2:OPHIO1 to a 55 degrees C heat shock treatment, so...
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PMID: 18255325
PDF is available here.
PDF is available here.
Microbiology (154)Pt 4 2008
Abstract
We found that the promoter formed by the complete junction P(jun) was more active than that formed by the 25 bp deleted junction P(djun) or the indigenous promoter P(IRL). The corresponding transcription start sites for both promoter P(jun) and P(IRL) as well as P(djun) were subsequently determined...
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PMID: 18375798
PDF is available here.
PDF is available here.