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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Transposases (1):
Recent article
Transposases
Transgenic Research (17)2 2008
Abstract
We cloned the promoters of four zebrafish genes that likely are transcribed ubiquitously throughout development and into the adult. These four genes are the TATA box binding protein gene, the taube nuss-like gene, the eukaryotic elongation factor 1-gamma gene, and the beta-actin-1 gene. We PCR ampli...
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PMID: 17968670
PDF is available here.
PDF is available here.
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Genetika (Moskva) (44)3 2008
Abstract
The effect on P-element activity in somatic cells was studied for a repair mutation localized in the Drosophila genome in the region of the rad201 and Rad51C overlapping genes. When one of the parents carried nonautonomous P elements and the rad201 mutation and the other carried a P-transposase sour...
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PMID: 18664138
PDF is available here.
PDF is available here.
Molecular Cell (29)3 2008
Abstract
We have reconstituted the entire set of transposition cleavage and strand transfer reactions in vitro and find that, unlike any other known transposition system, they strictly require single-strand DNA. TnpA, the shortest identified transposase, uses a nucleophilic tyrosine for these reactions. It r...
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PMID: 18280236
PDF is available here.
PDF is available here.
Abstract
I characterized an insertion sequence, termed ISWpi1, for which the taxonomic distribution appears to be restricted to the obligate intracellular alpha-Proteobacterium Wolbachia pipientis. ISWpi1 exhibits approximately 46% identity at the amino acid level with members of the IS1031 group of insertio...
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PMID: 18155858
PDF is available here.
PDF is available here.
Abstract
We investigated the transcriptional activities of SB in order to assess its potential to alter host gene expression upon integration. The untranslated regions (UTRs) of the transposon direct convergent, inward-directed transcription. Transcription from the 5'-UTR of SB is upregulated by the host-enc...
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PMID: 18071335
PDF is available here.
PDF is available here.
Microbiology (154)Pt 2 2008
Abstract
Xanthomonas axonopodis pv. citri (Xac) causes citrus canker and the completion of the Xac genome sequence has opened up the possibility of investigating basic cellular mechanisms at the genomic level. Copper compounds have been extensively used in agriculture to control plant diseases. The copA and...
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PMID: 18227244
PDF is available here.
PDF is available here.
Abstract
Because of Burkholderia pseudomallei's classification as a select agent in the United States, genetic manipulation of this bacterium is strictly regulated. Only a few antibiotic selection markers, including gentamicin, kanamycin, and zeocin, are currently approved for use with this bacterium, but wi...
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PMID: 18156318
PDF is available here.
PDF is available here.
Molecular Microbiology (67)3 2008
Abstract
We describe the isolation and analysis of mutant transposases that have, for the first time, provided genetic and biochemical evidence for the stage-specific positioning of both donor and target DNAs within the synaptic complex. Furthermore, we have provided evidence that some of the amino acids tha...
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PMID: 18086215
PDF is available here.
PDF is available here.
Abstract
The subtractive hybridization technique was effective in screening the two strains for specific DNA sequences, some of which might be responsible for determining virulence. The results suggested that several genes specific to strain W83 were associated with its virulence. Further analysis of these D...
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PMID: 18173803
PDF is available here.
PDF is available here.
Insect Molecular Biology (17)1 2008
Abstract
Two piggyBac-like elements (PLEs) were identified in the cotton bollworm, Helicoverpa armigera, and were designated as HaPLE1 and HaPLE2. HaPLE1 is flanked by 16 bp inverted terminal repeats (ITRs) and the duplicated TTAA tetranucleotide, and contains an open reading frame (ORF) of 1794 bp with the...
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PMID: 18237280
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)4 2008
Abstract
A series of Tn5 transposases (Tnp's) with mutations at the conserved amino acid position W450, which was structurally predicted to be important for synapsis, have been generated and characterized. This study demonstrates that W450 is involved in hydrophobic (and possibly aromatic) contacts within th...
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PMID: 18083803
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)4 2008
Abstract
We demonstrated that Escherichia coli tolC mutations reduce the high-level resistance to tetracycline afforded by the transposon Tn10-encoded TetA pump from resistance at 200 microg/ml to resistance at 40 microg/ml. In this study, we found that the addition of an sbmA mutation to a tolC::Tn10 mutant...
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PMID: 18083810
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)4 2008
Abstract
The mobile element IS256 causes phase variation of biofilm formation in Staphylococcus epidermidis by insertion and precise excision from the icaADBC operon. Precise excision, i.e., removal of the target site duplications (TSDs) and restoration of the original DNA sequence, occurs rarely but indepen...
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PMID: 18065530
PDF is available here.
PDF is available here.
Abstract
We show how the interplay of protein and DNA activates TnpA, the Helicobacter pylori IS608 transposase, for catalysis. First, transposon end binding causes a conformational change that aligns catalytically important protein residues within the active site. Subsequent precise cleavage at the left and...
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PMID: 18243097
PDF is available here.
PDF is available here.
Journal of Molecular Biology (375)2 2008
Abstract
We demonstrate that sub-terminal sequences adjacent to the inverted repeats of IS30 are also required for optimal transposition activity. We have developed a cell-free recombination system and showed that the transposase catalyses formation of a figure-of-eight transposition intermediate, where a 2...
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PMID: 18022196
PDF is available here.
PDF is available here.
Developmental Dynamics (237)1 2008
Abstract
We created transposase-expressing lines of Minos in Ciona. A Ciona gene encoding protamine (Ci-prm) is expressed in the testes and sperm. Transgenic lines expressing Minos transposase in the testes and sperm were created with a cis-element of Ci-prm, and used for enhancer detection. Double-transgeni...
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PMID: 17948255
PDF is available here.
PDF is available here.
Abstract
We provide an overview of some general issues related to use of Sleeping Beauty for cancer genetic studies and present here the polymerase chain reaction-based method for cloning transposon-tagged sequences from tumors....
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PMID: 18370070
PDF is available here.
PDF is available here.
Abstract
We provide experimental protocols for the construction, functional testing, and application of the Sleeping Beauty transposon for insertional mutagenesis of the mouse germline....
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PMID: 18370071
PDF is available here.
PDF is available here.
Abstract
We describe methods that we have developed for testing SB-mediated transposition, first in cultured mammalian cells, and then in vivo using a combination of rapid, high-volume tail vein injection for DNA delivery to the liver along with in vivo bioluminescence imaging to monitor sustained luciferase...
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PMID: 18470650
PDF is available here.
PDF is available here.
Abstract
We describe strategies for conditional expression systems that would facilitate further analysis of this class of genes. Finally, we outline a method based on the approach used in H. influenzae for identifying essential genes that can be applied to other bacteria that are not naturally transformable...
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PMID: 18392959
PDF is available here.
PDF is available here.
Abstract
The development of a technique to stably integrate exogenous DNA into the germline of Drosophila melanogaster marked a milestone in the ability to study gene function in the fly. On the molecular level germline transformation mainly relies on a particular transposable element, the D. melanogaster P-...
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PMID: 18641941
PDF is available here.
PDF is available here.
Abstract
The GFP gene controlled by a mini-promoter of the gene was cloned into a modified nonautonomous transposon Tol2 from the medaka genome. Such transposon cannot itself transpose, but external transposase allows it to integrate into the germ cell genome, where it remains after transposase is depleted....
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PMID: 18669290
PDF is available here.
PDF is available here.
Abstract
We took advantage of the vast amount of sequence information that has become recently available for a wide range of fungal and animal species to investigate the origin of the mammalian CENP-B and yeast CENP-B-like genes. A highly conserved ortholog of CENP-B was detected in 31 species of mammals, in...
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PMID: 17940212
PDF is available here.
PDF is available here.
Abstract
A total of 227 IS4-related sequences were collected among more than 500 sequenced bacterial and archaeal genomes, representing more than a three fold increase of the initial inventory. A clear division into seven coherent subgroups was discovered as well as three emerging families, which displayed d...
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PMID: 18215304
PDF is available here.
PDF is available here.
BMC Genomics (9)1 2008
Abstract
Here, we report the identification of two novel Entamoeba-specific repeats: ERE1 and ERE2; ERE1 is spread across the three genomes and associated with different repeats in a species-specific manner, while ERE2 is unique to E. histolytica. We also report the identification of two novel subfamilies of...
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PMID: 19077187
PDF is available here.
PDF is available here.