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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Integrases (1):
Recent article
Integrases
Abstract
We report the generation and molecular characterization of an EphA4 conditional allele along with a novel null allele with a knockin fluorescent reporter gene (mCFP). The conditional allele will be useful in ascertaining postdevelopmental and/or cell type-specific function of EphA4 in physiology, in...
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PMID: 20014422
PDF is available here.
PDF is available here.
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Abstract
We generated a mouse model that enables conditional ablation of PR function in a spatiotemporal specific manner. Exon 2 of the murine PR gene was floxed to generate a conditional PR allele (PR(flox)) in mice. Crossing the PR(flox/flox) mouse with the ZP3-cre transgenic demonstrated that the PR(flox)...
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PMID: 20029965
PDF is available here.
PDF is available here.
Abstract
We generated transgenic mouse line C57BL/6-Tg(Hspa2-cre)1Eddy/J (Hspa2-cre), which expresses cre-recombinase under the control of a 907-bp fragment of the heat shock protein 2 (Hspa2) gene promoter. Transgene expression was determined using Gt(ROSA)26Sor(tm1Sor)/J (ROSA26) and Tg(CAG-Bgeo/GFP)21Lbe/...
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PMID: 20027617
PDF is available here.
PDF is available here.
Abstract
I have examined the feasibility of the use of phiC31 integrase for intramolecular recombination strategies in zebrafish embryos. I report here that (1) phiC31 integrase is functional in zebrafish cells, (2) phiC31 integrase can excise a transgene cassette flanked by an attB and an attP site, analogo...
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PMID: 20094996
PDF is available here.
PDF is available here.
Abstract
We recently demonstrated that expression of (V600E)Braf in mature mouse melanocytes induces melanoma. Here, we show that expression of (V600E)Braf using the tyrosinase promoter leads to an unexpected embryonic lethality, with the animals dying before, at, or shortly after birth. The mice suffer from...
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PMID: 20002887
PDF is available here.
PDF is available here.
Abstract
We therefore conducted an extensive in silico study of class 1 integron sequences in order to assess the distribution of Pc variants. We also measured these promoters' strength by means of transcriptional reporter gene fusion experiments and estimated the excision and integration activities of the d...
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PMID: 20066027
PDF is available here.
PDF is available here.
Abstract
We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology i...
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PMID: 19966808
PDF is available here.
PDF is available here.
Abstract
We demonstrate the use of an adenovirus containing the Cre recombinase gene to infect primary mouse embryonic fibroblasts (MEFs) obtained from embryos containing a floxed Rac1 allele. Our rationale for selecting Rac1 MEFs for our experiments is that clear morphological changes can be seen upon delet...
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PMID: 20864926
PDF is available here.
PDF is available here.
Abstract
Lentiviral vectors are a powerful tool for gene transfer into target cells in vitro and in vivo. However, there are concerns about safety with regard to their use in gene transfer protocols because of insertional mutagenesis following viral infection. Once in the target cells, and in addition to the...
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PMID: 20225038
PDF is available here.
PDF is available here.
Abstract
Integrons gained great interest due to their participation in resistance gene recruitment and expression. Their basic structure includes a fragment that encodes an integrase (intI) followed by a recognition sequence (attI) into which they may incorporate gene cassettes (encoding resistance mechanism...
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PMID: 20461298
PDF is available here.
PDF is available here.
médecine/sciences (26)1 2010
Abstract
Nature Neuroscience (13)1 2010
Abstract
We have generated a set of Cre reporter mice with strong, ubiquitous expression of fluorescent proteins of different spectra. The robust native fluorescence of these reporters enables direct visualization of fine dendritic structures and axonal projections of the labeled neurons, which is useful in...
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PMID: 20023653
PDF is available here.
PDF is available here.
BMC Biotechnology (10) 2010
Abstract
We therefore evaluated the utility of Real-Time PCR to measure 1lox/2lox allele ratios.
We show that SYBR Green based Real-Time PCR analysis of 1lox/2lox allele ratios can generate erroneous peaks in the melting curve that are possibly caused by alternate hybridization products promoted by the palin...
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PMID: 20950424
PDF is available here.
PDF is available here.
Abstract
In this study, we investigated the potential benefits of overall MEF2 inhibition in a mouse model of chronic pressure overloading, by subjecting transgenic mice expressing a dominant negative form of MEF2 (DN-MEF2 Tg) in the heart, to transverse aortic constriction (TAC). Histological analysis revea...
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PMID: 20023039
PDF is available here.
PDF is available here.
BMC Biotechnology (10) 2010
Abstract
Using ES cells, we compared six RE mutant lox sites, focusing on their recombination efficiency with lox71. All of the RE mutant lox sites showed similar recombination efficiency. We then analyzed the stability of the recombined product, i.e., the LE+RE double mutant lox site, under continuous and s...
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PMID: 20356367
PDF is available here.
PDF is available here.
Abstract
We developed a method for delivering RU486, the conditional activator of Cre recombinase progesterone receptor (CrePR) fusion proteins to the lung and then examined the activity of three keratin-driven CrePR constructs in the conducting airways. We also developed a technique for survival bronchioalv...
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PMID: 20140084
PDF is available here.
PDF is available here.
BMC Microbiology (10) 2010
Abstract
The system established in this study allows us to express an N-terminal epitope tagged gene from its own endogenous promoter in Tetrahymena....
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PMID: 20626890
PDF is available here.
PDF is available here.
Abstract
We describe recombination-induced tag exchange (RITE), a genetic method that induces a permanent epitope-tag switch in the coding sequence after a hormone-induced activation of Cre recombinase. The time-controlled tag switch provides a unique ability to detect and separate old and new proteins in ti...
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PMID: 20018668
PDF is available here.
PDF is available here.
Abstract
We have developed a fast and efficient site-specific system of integrative recombination into the P. falciparum genome, which is catalyzed by the mycobacteriophage Bxb1 serine integrase. This system has the advantage of providing greater genetic and phenotypic homogeneity within transgenic lines as...
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PMID: 20676977
PDF is available here.
PDF is available here.
Journal of Virology (84)1 2010
Abstract
We correlate infectivity defects in transportin 3 knockdown cells with in vitro protein binding affinities for an expanded set of retroviruses that include simian immunodeficiency virus (SIV), bovine immunodeficiency virus (BIV), equine infectious anemia virus (EIAV), feline immunodeficiency virus (...
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PMID: 19846519
PDF is available here.
PDF is available here.
Molecular Pain (6)1 2010
Abstract
We deleted ephrin-B2 in Nav1.8+ nociceptive sensory neurons with the Cre-loxP system. Sensory neuron numbers and terminals were examined using neuronal makers. Pain behavior in acute, inflammatory and neuropathic pain models was assessed in the ephrin-B2 conditional knockout (CKO) mice. We also inve...
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PMID: 21059214
PDF is available here.
PDF is available here.
Molecular Pain (6)1 2010
Abstract
We deleted ephrin-B2 in Nav1.8+ nociceptive sensory neurons with the Cre-loxP system. Sensory neuron numbers and terminals were examined using neuronal makers. Pain behavior in acute, inflammatory and neuropathic pain models was assessed in the ephrin-B2 conditional knockout (CKO) mice. We also inve...
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PMID: 21059214
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We therefore conducted an extensive in silico study of class 1 integron sequences in order to assess the distribution of Pc variants. We also measured these promoters' strength by means of transcriptional reporter gene fusion experiments and estimated the excision and integration activities of the d...
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PMID: 20066027
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We therefore conducted an extensive in silico study of class 1 integron sequences in order to assess the distribution of Pc variants. We also measured these promoters' strength by means of transcriptional reporter gene fusion experiments and estimated the excision and integration activities of the d...
|
PMID: 20066027
PDF is available here.
PDF is available here.
PLoS Genetics (6)1 2010
Abstract
We therefore conducted an extensive in silico study of class 1 integron sequences in order to assess the distribution of Pc variants. We also measured these promoters' strength by means of transcriptional reporter gene fusion experiments and estimated the excision and integration activities of the d...
|
PMID: 20066027
PDF is available here.
PDF is available here.