Find your PDFs fast.
Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Recombinases (5):
Recent article
Recombinases
Abstract
We deleted the cohesin locus Rad21 in mouse thymocytes at a time in development when these cells stop cycling and rearrange their T-cell receptor (TCR) α locus (Tcra). Rad21-deficient thymocytes had a normal lifespan and retained the ability to differentiate, albeit with reduced efficiency. Loss of...
|
PMID: 21832993
PDF is available here.
PDF is available here.
| < More recent | Older article > |
Kidney International (79)5 2011
Abstract
For nearly 100 years, developmental biologists have utilized fate mapping to understand the contributions of progenitor populations to organogenesis. More recently, Cre-Lox technology has allowed genetic fate mapping in adult mice, clarifying cell hierarchies in adult kidney disease...
|
PMID: 20861816
PDF is available here.
PDF is available here.
Kidney International (79)5 2011
Abstract
For nearly 100 years, developmental biologists have utilized fate mapping to understand the contributions of progenitor populations to organogenesis. More recently, Cre-Lox technology has allowed genetic fate mapping in adult mice, clarifying cell hierarchies in adult kidney disease...
|
PMID: 20861816
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)30 2010
Abstract
We showed that substitution of the scissile phosphate (P) by the charge neutral methylphosphonate (MeP) makes Arg-308 dispensable during the catalytic activation of the MeP diester bond. However, in the Flp(R308A) reaction, water out-competes the tyrosine nucleophile (Tyr-343) to cause direct hydrol...
|
PMID: 20448041
PDF is available here.
PDF is available here.
Journal of Bacteriology (192)12 2010
Abstract
We sought to identify the relative contributions of CcrA and CcrB in the excision and integration of SCCmec. Purified CcrB but not CcrA was shown to mediate the gel shift of chromosomal target integration sequences (attB) in electrophoretic mobility shift assays. However, preincubation of CcrB-DNA c...
|
PMID: 20382769
PDF is available here.
PDF is available here.
Biotechnology Letters (32)4 2010
Abstract
We obtained T7 RNA polymerase (RNAP) substitution mutants in Burkholderia cepacia. To test the expression abilities of the T7 mutants, four different lipase expression vectors were transformed and the lipase activity of these recombinants was evaluated. Our results suggest that 500 nt homology betwe...
|
PMID: 20033831
PDF is available here.
PDF is available here.
Abstract
We are using a combination of X-ray crystallography, biochemistry and genetics to model the full synaptic complex and to understand how the regulatory portion activates the crossover-site-bound recombinases....
|
PMID: 20298188
PDF is available here.
PDF is available here.
Abstract
The active-site interactions involved in the catalysis of DNA site-specific recombination by the serine recombinases are still incompletely understood. Recent crystal structures of synaptic gammadelta resolvase-DNA intermediates and biochemical analysis of Tn3 resolvase mutants have provided new ins...
|
PMID: 20298194
PDF is available here.
PDF is available here.
Abstract
Most temperate phages encode an integrase for integration and excision of the prophage. Integrases belong either to the lambda Int family of tyrosine recombinases or to a subgroup of the serine recombinases, the large serine recombinases. Integration by purified serine integrases occurs efficiently...
|
PMID: 20298189
PDF is available here.
PDF is available here.
Abstract
Various types of the staphylococcal cassette chromosome mec (SCCmec) are known to confer methicillin resistance on the human pathogen Staphylococcus aureus. Such cassettes are not always stably maintained. The present studies were aimed at identifying the mechanism underlying the in vivo conversion...
|
PMID: 19995931
PDF is available here.
PDF is available here.
Journal of Bacteriology (192)3 2010
Abstract
We have identified and functionally characterized the RDF involved in site-specific excision of the prophage genome. The mv4 RDF, (mv4)Xis, is encoded by the second gene of the early lytic operon. It is a basic protein of 56 amino acids. Electrophoretic mobility shift assays demonstrated that (mv4)X...
|
PMID: 19948798
PDF is available here.
PDF is available here.
Abstract
We report the identification of Slx4 orthologs in metazoa, including fly MUS312, essential for meiotic recombination, and human BTBD12, an ATM/ATR checkpoint kinase substrate. Human SLX1-SLX4 displays robust Holliday junction resolvase activity in addition to 5' flap endonuclease activity. Depletion...
|
PMID: 19596236
PDF is available here.
PDF is available here.
Molecular Cell (35)1 2009
Abstract
We determined that the Drosophila MEI-9-ERCC1 endonuclease interacts with the MUS312 protein to produce meiotic crossovers, and that MUS312 has a MEI-9-independent role in interstrand crosslink (ICL) repair. The importance of MUS312 to pathways crucial for maintaining genomic stability in Drosophila...
|
PMID: 19595722
PDF is available here.
PDF is available here.
Transgenic Research (18)3 2009
Abstract
We herein describe the successful excision of antibiotic resistance genes from transgenic plants via the use of an oxidative stress-inducible FLP gene. FLP encodes a recombinase that can eliminate FLP and hpt selection genes flanked by two FRT sites. During a transformation procedure in tobacco, tra...
|
PMID: 19160066
PDF is available here.
PDF is available here.
Journal of Biotechnology (142)2 2009
Abstract
Helper-dependent adenoviral vectors (HDVs) are the most promising adenoviral vectors for gene therapy treatments as well as vaccination strategies. However, the lack of a robust and efficient strategy to produce the HDV at high titers constitutes a major obstacle hindering the use of...
|
PMID: 19501266
PDF is available here.
PDF is available here.
EMBO Journal (28)6 2009
Abstract
We show that TRF2 greatly increases the rate of Holliday junction (HJ) formation and blocks the cleavage by various types of HJ resolving activities, including the newly identified human GEN1 protein. By using potassium permanganate probing and differential scanning calorimetry, we reveal that the b...
|
PMID: 19197240
PDF is available here.
PDF is available here.
Abstract
This study was undertaken to identify an unknown resolvase in an E. coli strain lacking RuvABC (N4237) by using transposon mutagenesis. One out of 10000 clones was retained for further study as it was resistant to UV light and mitomycin C. The result of transductional mapping and PCR sequencing show...
|
PMID: 19580006
PDF is available here.
PDF is available here.
Molecular Microbiology (71)5 2009
Abstract
We report that the relationship of ResT to the tyrosine recombinases extends to the ability to synapse-replicated telomeres and to catalyse the formation of a Holliday junction. We also report that ResT can use asymmetrized substrates that mimic the properties of a recombination site for a tyrosine...
|
PMID: 19170885
PDF is available here.
PDF is available here.
Plant Cell Reports (28)2 2009
Abstract
We obtained a stable transformation frequency of 9.8% with pIPT10 construct. The ipt-type MAT vector pEXM2 was then used to monitor the excision events mediated by the yeast Recombinase and the consequent production of ipt marker-free transgenic plants. Transgenic ipt-shooty lines were recovered at...
|
PMID: 19011862
PDF is available here.
PDF is available here.
Abstract
Staphylococcal cassette chromosome mec (SCCmec) type VII, found in community-acquired methicillin-resistant Staphylococcus aureus belonging to multilocus sequence type (ST) 59 from Taiwan, was 41,347bp in size and flanked by 19-bp attL and attR sequences. It was inserted into the att site at the 3'-...
|
PMID: 18926798
PDF is available here.
PDF is available here.
Abstract
We outline a method for identifying and characterizing from a total lysogen population, the subpopulation in which the prophage is induced. The prophage is genetically altered so that on induction it does not go through the lytic pathway, but does express a resolvase that acts at a reporter cassette...
|
PMID: 19066826
PDF is available here.
PDF is available here.
Molecular Cell (32)5 2008
Abstract
The Holliday junction (HJ) is a central intermediate in homologous recombination. Ip et al. (2008), in a recent issue of Nature, have identified a new subclass of the Rad2/XPG family of nucleases with the hallmark of symmetrical cleavage of HJ substrates to produce nicked duplex products.
|
PMID: 19061635
PDF is available here.
PDF is available here.
Cellular Signalling (20)11 2008
Abstract
We created isogenic embryonic stem cell (ESC) lines expressing various alleles of PDK1 within a PDK1-/- background. Disruption of the NES domain of PDK1 correlated with elevated PKB phosphorylation at both T308 and S473. In contrast, mutation of S396 to alanine reduced PDK1 nuclear localization and...
|
PMID: 18718528
PDF is available here.
PDF is available here.
Abstract
Nucleic Acids Research (36)13 2008
Abstract
We expressed the Oryza sativa DMC1A and DMC1B proteins in bacteria and purified them. The purified DMC1A and DMC1B proteins formed helical filaments along single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA), and promoted robust strand exchange between ssDNA and dsDNA over five thousand base...
|
PMID: 18583359
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (284)1 2008
Abstract
We report two strategies, a recombinase-based reporter system and an antisense technology, to study gene expression and essentiality in hypoxic nonreplicating mycobacteria. The recombinase-based reporter system relies on the resolution of an antibiotic marker flanked by the gammadelta-res sites. Thi...
|
PMID: 18544099
PDF is available here.
PDF is available here.
Nucleic Acids Research (36)12 2008
Abstract
We determined the crystal structure of the human DMC1-M200V variant in the octameric-ring form. Biochemical analyses revealed that the human DMC1-M200V variant had reduced stability, and was moderately defective in catalyzing in vitro recombination reactions. The corresponding M194V mutation introdu...
|
PMID: 18566005
PDF is available here.
PDF is available here.
Cloning and Stem Cells (10)2 2008
Abstract
We report multiple sequential modifications of an endogenous gene (hprt) in hESCs. A selectable marker flanked by heterospecific lox sites was first introduced by homologous recombination (HR) into the hprt gene. In a subsequent step, exchange of the selectable marker with another cassette was achie...
|
PMID: 18386992
PDF is available here.
PDF is available here.
Hepatology (47)6 2008
Abstract
We recently reported that mice with a T cell-restricted expression of a dominant negative form of transforming growth factor beta receptor type II (dnTGFbetaRII) spontaneously develop autoimmune cholangitis that resembles human primary biliary cirrhosis (PBC), including antimitochondrial antibodies...
|
PMID: 18452147
PDF is available here.
PDF is available here.
Abstract
We show that, analogously to the pTalpha chain, surface CNX is palmitoylated and that CD3 prominently accumulated in lipid rafts upon cross-linking. Mutant CNX isoforms devoid of ER retention determined pre-TCR-like signaling and simulated beta selection only when stably translocating CD3 to lipid r...
|
PMID: 18350545
PDF is available here.
PDF is available here.
Archives of Microbiology (189)4 2008
Abstract
We observed the ability of XisA to excise substrate plasmids that contained the flanking regions of the nifD element in an E. coli host. Using PCR directed mutagenesis, nucleotides in the nifD element flanking regions in substrate plasmids were altered and the effect on recombination was determined....
|
PMID: 18283436
PDF is available here.
PDF is available here.
Abstract
Molecular Microbiology (67)6 2008
Abstract
We demonstrate that all six Vpma proteins are expressed in the type strain PG2 and all undergo phase variation at an unusually high frequency. Furthermore, targeted gene disruption of the xer1 gene encoding a putative site-specific recombinase adjacent to the vpma locus was accomplished via homologo...
|
PMID: 18248580
PDF is available here.
PDF is available here.
Molecular Microbiology (67)5 2008
Abstract
We report here a powerful method for introducing single point mutations into mycobacterial genomes using oligonucleotide-derived single-stranded DNA recombineering and mycobacteriophage-encoded proteins. Phage Che9c gp61-mediated recombination is sufficiently efficient that single base changes can b...
|
PMID: 18221264
PDF is available here.
PDF is available here.
Molecular Microbiology (67)5 2008
Abstract
We show that, by two mechanisms, during natural transformation of Acinetobacter baylyi two IR events can integrate DNA segments. One mechanism is double illegitimate recombination (DIR) acting in the absence of any homology (frequency: 7 x 10(-13) per cell). It occurs about 10(10)-fold less frequent...
|
PMID: 18194157
PDF is available here.
PDF is available here.
Abstract
We report the identification and functional analysis of nine genes from Gram-positive and Gram-negative bacteria and their phages that are similar to lambda (lambda) bet or Escherichia coli recT. Beta and RecT are single-strand DNA annealing proteins, referred to here as recombinases. Each of the ni...
|
PMID: 18230724
PDF is available here.
PDF is available here.
Abstract
We adapted TnpR recombinase-based in vivo expression technology (RIVET) to document gene regulation in Sinorhizobium meliloti. The substrate for TnpR, the res1-tet-res1 cassette, is stably inherited when cloned into a neutral site of the S. meliloti genome. Bicistronic promoterless tnpR-beta-glucuro...
|
PMID: 18184060
PDF is available here.
PDF is available here.
Development (135)4 2008
Abstract
We have established mice in which gene cassettes can be efficiently knocked-in into the Ednra locus using recombinase-mediated cassette exchange (RMCE) based on the Cre-lox system. The first homologous recombination introducing mutant lox-flanked Neo resulted in homeotic transformation of the lower...
|
PMID: 18199583
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)3 2008
Abstract
We show that this organism also produces a high-molecular-weight extracellular polysaccharide (EPS). Expression of the EPS results in the formation of a large polysaccharide layer around the bacteria which prevents them from forming a tight pellet upon centrifugation and from entering a Percoll dens...
|
PMID: 18039760
PDF is available here.
PDF is available here.
Abstract
We report the generation of Tre recombinase through directed, molecular evolution. Tre recombinase recognizes a pre-defined target sequence within the LTR sequences of the HIV-1 provirus, resulting in the excision and eradication of the provirus from infected human cells. We started with Cre, a 38-k...
|
PMID: 19066582
PDF is available here.
PDF is available here.
Abstract
We report that the engineered recombinase Tre (see Molecular evolution of the Tre recombinase, Buchholz, F., Max Planck Institute for Cell Biology and Genetics, Dresden) efficiently excises integrated HIV-1 proviral DNA from the genome of infected cells. We produced loxLTR containing viral pseudotyp...
|
PMID: 19066545
PDF is available here.
PDF is available here.
Abstract
In this work, we describe the design and synthesis of an inversion switch using the fim and hin inversion recombination systems to create a heritable sequential memory switch. We have integrated the two inversion systems in an overlapping manner, creating a switch that can have multiple states. The...
|
PMID: 18665232
PDF is available here.
PDF is available here.
Abstract
We have no intention to cover the entire field but will instead focus on most recent vectors and protocols, which have turned out to be most useful in order to promote the technology. Therefore, we apologize upfront to the many studies that could not be mentioned here solely owing to space limitatio...
|
PMID: 18370072
PDF is available here.
PDF is available here.
Abstract
We first showed that S. acidocaldarius can repair DNA damage induced by high doses of gamma rays, and we performed a time course analysis of the total levels and sub-cellular partitioning of Rad50, Mre11, HerA and NurA along with the RadA recombinase in both control and irradiated cells. We found th...
|
PMID: 18294364
PDF is available here.
PDF is available here.
Abstract
This system has been modified for use in Pseudomonas aeruginosa. Chromosomal DNA deletions of single genes were generated using 3-step PCR products containing flanking regions 400-600 nucleotides (nt) in length that are homologous to the target sequence. A 1-step PCR product with a homologous extens...
|
PMID: 18248677
PDF is available here.
PDF is available here.
BMC Genomics (9)1 2008
Abstract
H. akashiwo strain CCMP452 (West Atlantic) chloroplast DNA is 160,149 bp in size with a 21,822-bp inverted repeat, whereas NIES293 (West Pacific) chloroplast DNA is 159,370 bp in size and has an inverted repeat of 21,665 bp. The fosmid cloning technique reveals that both strains contain an isomeric...
|
PMID: 18462506
PDF is available here.
PDF is available here.
Molecular Microbiology (67)1 2008
Abstract
We examined the mechanisms by which a conserved PAI contributes to UPEC pathogenesis in acute cystitis. In the human UPEC strain UTI89, spontaneous excision of PAI II(UTI89) disrupts the adjacent leuX tRNA locus. Loss of wild-type leuX-encoded tRNA(5)(Leu) significantly delayed, but did not eliminat...
|
PMID: 18036139
PDF is available here.
PDF is available here.
Molecular Microbiology (67)1 2008
Abstract
We have found that FimB and FimE invert the switch through a common mechanism. A genetic investigation involving base-by-base substitution combined with a biochemical study shows that the same DNA cleavage and religation sites are used within the 9 bp inverted repeats, and that each recombination in...
|
PMID: 18034794
PDF is available here.
PDF is available here.
Abstract
We have devised a recombinase-based promoter-trapping strategy to identify genes with elevated expression during pig infection with Salmonella enterica serovar Typhimurium. A total of 55 clones with in vivo-induced promoters were selected from a genomic library of approximately 10,000 random Salmone...
|
PMID: 17921269
PDF is available here.
PDF is available here.
Microbiology (153)Pt 12 2007
Abstract
FimB and FimE are site-specific recombinases, part of the lambda integrase family, and invert a 314 bp DNA switch that controls the expression of type 1 fimbriae in Escherichia coli. FimB and FimE differ in their activity towards the fim switch, with FimB catalysing inversion in both directions in c...
|
PMID: 18048927
PDF is available here.
PDF is available here.
Abstract
We developed a simple method of generating markerless deletions in the Escherichia coli chromosome. The method consists of two recombination events stimulated by lambda Red recombinase. The first recombination replaced a target region with a marker cassette and the second then eliminated the marker...
|
PMID: 18071259
PDF is available here.
PDF is available here.
Abstract
We have tested if reducing the efficiency of the packaging process of the helper adenovirus could impair its packaging without affecting helper-dependent adenovirus production. Interestingly, insertion of attB/attP-PhiC31 sequences flanking the packaging signal significantly lengthens adenovirus cyc...
|
PMID: 17560622
PDF is available here.
PDF is available here.
Abstract
In the past years, recombinase-based approaches for integrating transgenes into defined chromosomal loci of mammalian cells have gained increasing attention. This method is attractive since it enables to precisely integrate transgenes of interest into pre-defined integration sites, thereby allowing...
|
PMID: 17904350
PDF is available here.
PDF is available here.
Abstract
BmNPV bacmid constructed recently and Red recombinant system were used to rapidly disrupted Bombyx monri nucleopolyhedrovirus (BmNPV) orf60 in Escherichia coli (E. coli) BW25113. BmNPV bacmid isolated from E. coli BmDH10Bac was electroporated into E. coli BW25113, which harbors plasmid pKD46 encodin...
|
PMID: 18051855
PDF is available here.
PDF is available here.
Journal of Bacteriology (189)18 2007
Abstract
We found that changing three of the six conserved amino acids in the signature, one of which was the presumed catalytic tyrosine, resulted in a 1,000-fold decrease in integration frequency. Changes in the other amino acids had little or no effect. Thus, although the CTnBST integrase still seems to b...
|
PMID: 17616597
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)35 2007
Abstract
We examine the fate of the helicase and both DNA polymerases when the leading strand polymerase is blocked. We find that the helicase continues over 0.5 kb but less than 3 kb and that the lagging strand DNA polymerase remains active despite its connection to a stalled leading strand enzyme. Furtherm...
|
PMID: 17609212
PDF is available here.
PDF is available here.
Abstract
We show here that Streptococci and Lactococci carry an alternative Xer recombination machinery, organized in a single recombination module. This corresponds to an atypical 31-bp recombination site (dif(SL)) associated with a dedicated tyrosine recombinase (XerS). In contrast to the E. coli Xer syste...
|
PMID: 17630835
PDF is available here.
PDF is available here.
Abstract
We describe a novel approach for the rapid generation of reporter gene fusions in single copies at defined positions in bacterial genomes. This technique utilizes the Red recombinase for the homologous recombination of PCR-generated cassettes containing various currently used reporter genes, such as...
|
PMID: 17513596
PDF is available here.
PDF is available here.
Trends in Microbiology (15)7 2007
Abstract
We give an overview of the structural and phylogenetic diversity of integrons and describe evolutionary events that have contributed to the success of these genetic elements....
|
PMID: 17566739
PDF is available here.
PDF is available here.
Abstract
Recombineering, a new genetic engineering technology based on high efficiency in vivo homologous recombination, can be used in target DNA knock-in, knock-out and gene cloning. In the process of gene subcloning mediated by Recombineering technique, high-quality target DNA fragments were difficult to...
|
PMID: 17822029
PDF is available here.
PDF is available here.